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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/30625?offset=1110</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27961/nearhgt</guid>
	<pubDate>Wed, 22 Jun 2016 05:41:57 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27961/nearhgt</link>
	<title><![CDATA[NearHGT]]></title>
	<description><![CDATA[<p>Horizontal gene transfer (HGT), the transfer of genetic material between organisms, is crucial for genetic innovation and the evolution of genome architecture. Existing HGT detection algorithms rely on a strong phylogenetic signal distinguishing the transferred sequence from ancestral (vertically derived) genes in its recipient genome. Detecting HGT between closely related species or strains is challenging, as the phylogenetic signal is usually weak and the nucleotide composition is normally nearly identical. Nevertheless, there is a great importance in detecting HGT between congeneric species or strains, especially in clinical microbiology, where understanding the emergence of new virulent and drug-resistant strains is crucial, and often time-sensitive.</p>
<p>We developed a novel, self-contained technique named&nbsp;<em>Near HGT</em>, based on the&nbsp;<em>synteny index</em>, to measure the divergence of a gene from its native genomic environment and used it to identify candidate HGT events between closely related strains. The method confirms candidate transferred genes based on the&nbsp;<em>constant relative mutability</em>&nbsp;(CRM). Using CRM, the algorithm assigns a confidence score based on &ldquo;unusual&rdquo; sequence divergence. A gene exhibiting exceptional deviations according to both synteny and mutability criteria, is considered a validated HGT product. We first employed the technique to a set of three&nbsp;<em>E. coli</em>&nbsp;strains and detected several highly probable horizontally acquired genes. We then compared the method to existing HGT detection tools using a larger strain data set.</p>
<p>When combined with additional approaches our new algorithm provides richer picture and brings us closer to the goal of detecting all newly acquired genes in a particular strain.</p>
<p><strong>Availability:</strong><span>&nbsp;The method is publicly available at</span><a href="http://research.haifa.ac.il/~ssagi/software/nearHGT.zip">http://research.haifa.ac.il/~ssagi/software/nearHGT.zip</a></p><p>Address of the bookmark: <a href="http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1004408" rel="nofollow">http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1004408</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/28425/advertisement-for-junior-research-fellowjrf-at-school-of-computational-and-integrative-sciences-jawaharlal-nehru-university</guid>
  <pubDate>Thu, 14 Jul 2016 07:24:53 -0500</pubDate>
  <link></link>
  <title><![CDATA[Advertisement for Junior Research Fellow(JRF)  at School of Computational and Integrative Sciences  Jawaharlal Nehru University]]></title>
  <description><![CDATA[
<p>Advertisement for Junior Research Fellow(JRF) - (1)</p>

<p>Applications are invited for a post in DST, India funded Project entitled: "Positive and negative impacts of macromolecular crowding agents during target site location by DNA binding proteins – origin of optimal search at physiological ionic concentration (Reference Number: ECR/2016/000188) ''. The selected candidate will be appointed purely on temporary basis, initially for two years as a JRF that may be extended to one year of SRF based on the performance.</p>

<p>Position: Junior Research Fellow (1)</p>

<p>Qualifications &amp; Experience: Candidate must have a consistently good academic record with at least 60% marks in all throughout and must have qualified NET/GATE.</p>

<p>Desirable: Basic knowledge in the field of biophysics, molecular simulations and computational biology are desirable.</p>

<p>Salary: Consolidated Rs. 25,000 per month.</p>

<p>Tenure: The project duration is for three years and the selected candidate would be appointed after an interview. Appointment will be purely on temporary basis as stipulated by the existing rules of the University.</p>

<p>Interested candidates need to send an application to the address mentioned below mentioning the name of the project and post applied for (on the cover of the envelope).</p>

<p>The applications along with CV should be mailed at the address given below. Name, address, contact number and e. mail address of two referees must be enclosed with the application. The last date for the application is July 31st 2016.</p>

<p>Dr. Arnab Bhattacharjee (Principal Investigator) <br />Assistant Professor <br />School of Computational and Integrative Sciences <br />Jawaharlal Nehru University <br />New Delhi-110067 <br />E-mail: arnab@jnu.ac.in</p>

<p>Note: 1. Only shortlisted candidates will be communicated to appear in the interview at SCIS, JNU and no other communications in this regard will be entertained.</p>

<p>2. No TA/DA will be paid for appearing in interview.</p>
]]></description>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/28547/jrf-bioinformatics-at-iit-delhi</guid>
  <pubDate>Mon, 25 Jul 2016 03:26:20 -0500</pubDate>
  <link></link>
  <title><![CDATA[JRF Bioinformatics at IIT, Delhi]]></title>
  <description><![CDATA[
<p>No. IITD/IRD/RP03017/4254/Advertisement No.: IITD/IRD/093/2016<br />JRF Bioinformatics  job vacancies in Indian Institute of Technology Delhi (IIT Delhi)<br />Title : Elucidation of Pathologically Relevant miRNAs Responsible for Disease Progression and Resistance to Chemotherapy in Chronic Lymphocytic Leukemia (CLL) (RP03017)<br />Qualification : Candidates having first class B. Tech. / M.Sc. Degree or equivalent in Bioinformatics or Biotechnology with NET qualification. Desirable: Candidates having computer programming skills (C++, Python, Java, Web designing using Materialize frameworks, database management, offline software GUI development) with knowledge of Linux server environment and / or experience in next generation sequencing (NGS) data analysis, MD simulations will be preferred.<br />No. of Post : 01<br />Pay Scale : Rs.25,000/-<br />How to apply<br />Walk-in test / interview will be held on 04/08/2016, 03.00 p.m. at Committee Room No. 230, Block-I, Department of Biochemical Engineering &amp; Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi-110016.</p>

<p>More at http://ird.iitd.ac.in/sites/default/files/jobs/project/IITD-IRD-093-2016.pdf</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/28566/emboss-apps</guid>
	<pubDate>Wed, 27 Jul 2016 06:00:41 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/28566/emboss-apps</link>
	<title><![CDATA[EMBOSS Apps]]></title>
	<description><![CDATA[<p>The programs are listed in alphabetical order, Look at the individual applications or go to the&nbsp;<a href="http://emboss.sourceforge.net/apps/release/6.6/emboss/apps/groups.html">GROUPS</a>&nbsp;page to search by category.</p>
<p><a href="http://emboss.sourceforge.net/apps/release/6.6/embassy/index.html">EMBASSY applications</a>&nbsp;are described in separate documentation for each package.</p>
<h3><a name="current" id="current"></a>Applications&nbsp;in the&nbsp;<a href="ftp://emboss.open-bio.org/pub/EMBOSS/">current release</a></h3><p>Address of the bookmark: <a href="http://emboss.sourceforge.net/apps/release/6.6/emboss/apps/" rel="nofollow">http://emboss.sourceforge.net/apps/release/6.6/emboss/apps/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/28602/srf-and-jrf-bioinformatics-at-tezpur-university-napaam</guid>
  <pubDate>Wed, 03 Aug 2016 03:47:38 -0500</pubDate>
  <link></link>
  <title><![CDATA[SRF and JRF Bioinformatics at Tezpur University, Napaam]]></title>
  <description><![CDATA[
<p>Applications are invited for the following temporary positions unde MHRD sponsored Centre of Excellence<br />in the Department of Computer Science and Engineering (CSE), Tezpur University<br /> <br />Qualification<br />and Experience : Senior Research Fellow (SRF) and JRF : First Class in M.E/M.Tech in CSE/IT/ECE with research<br />experience in relevant fields of research (Candidates having valid GATE/NET Score would be preferred).</p>

<p> <br />Fellowship: Rs. 18,000/- per month (fixed)<br /> <br />Duration : 2 (Two) years and may be extended<br />depending on status of the project<br /> <br />Age Limit: Candidates should not be more than 32 years of<br />age in case of SRF and 28 years of age in case of JRF and TA. Upper age limit may be relaxed up to 5<br />years in the case of candidate belonging to SC/ ST/ OBC/ Women/ Differently abled.<br /> <br />How to Apply:<br />Interested candidates may send their application on plain paper by post along with his/her educational<br />qualifications, research experience certificates (for SRF), 02 copies of recent passport/stamp size photographs<br />and contact phone number to Professor D.K Bhattacharyya, Principal Investigator, Department of Computer<br />Science &amp; Engineering, Tezpur University, Napaam – 784 028, or mail it to dkb@tezu.ernet.in<br />(or to smh@tezu.ernet.in) within 15 days of publication of this advertisement.<br /> <br />No TA/DA shall<br />be paid for attending the interview.<br /> <br />For more details: http://www.tezu.ernet.in/ProjectWalkin/Advt-DoRD-CSE-DKB-20-225-6779-A.pdf</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34246/unicycler-hybrid-assembly-pipeline-for-bacterial-genomes</guid>
	<pubDate>Fri, 10 Nov 2017 03:58:27 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34246/unicycler-hybrid-assembly-pipeline-for-bacterial-genomes</link>
	<title><![CDATA[Unicycler: Hybrid assembly pipeline for bacterial genomes]]></title>
	<description><![CDATA[<p><span>Unicycler is an assembly pipeline for bacterial genomes. It can assemble&nbsp;</span><a href="http://www.illumina.com/">Illumina</a><span>-only read sets where it functions as a&nbsp;</span><a href="http://cab.spbu.ru/software/spades/">SPAdes</a><span>-optimiser. It can also assembly long-read-only sets (</span><a href="http://www.pacb.com/">PacBio</a><span>&nbsp;or&nbsp;</span><a href="https://nanoporetech.com/">Nanopore</a><span>) where it runs a&nbsp;</span><a href="https://github.com/lh3/miniasm">miniasm</a><span>+</span><a href="https://github.com/isovic/racon">Racon</a><span>&nbsp;pipeline. For the best possible assemblies, give it both Illumina reads&nbsp;</span><em>and</em><span>&nbsp;long reads, and it will conduct a hybrid assembly.</span></p><p>Address of the bookmark: <a href="https://github.com/rrwick/Unicycler" rel="nofollow">https://github.com/rrwick/Unicycler</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/42490/bioinformatics-scientist-%E2%80%93-icmr-computational-genomics-centre</guid>
  <pubDate>Sat, 26 Dec 2020 10:18:29 -0600</pubDate>
  <link></link>
  <title><![CDATA[Bioinformatics Scientist – ICMR Computational Genomics Centre]]></title>
  <description><![CDATA[
<p>ICMR invites online applications, from Indian Citizens, up to 8th January 2020 till 5:30 PM to fill up the following post to be filled purely on a temporary basis under “ICMR Computational Genomics Centre” under Dr. Harpreet Singh, Head, Division of Biomedical Informatics (BMI), ICMR HQRS, New Delhi 110029.<br />The Terms &amp; Conditions for the post are as follows:</p>

<p>a) Scientist-B – UR (2 posts-Bioinformatics) on consolidated salary of Rs.48,000/- pm + HRA</p>

<p>b) Scientist C – UR (1 post -Bioinformatics) on consolidated salary of Rs. 51,000 pm+ HRA</p>

<p>c) Scientist B- UR (2 post-Statistics) on a consolidated salary of Rs.48,000/- pm +HRA</p>

<p>d) Computer Programmer 1 post UR &amp; 1 post SC on a consolidated salary of Rs. 32,500/- pm</p>

<p>e) Research Assistant -UR 1 post on a consolidated salary of Rs. 31,000/- pm</p>

<p>More at https://projectjobs.icmr.org.in/sccbioinformatics/uploads/recruitment/Adv_BMI_24122020.pdf</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/36621/hapcut2-robust-and-accurate-haplotype-assembly-for-diverse-sequencing-technologies</guid>
	<pubDate>Tue, 15 May 2018 07:35:26 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/36621/hapcut2-robust-and-accurate-haplotype-assembly-for-diverse-sequencing-technologies</link>
	<title><![CDATA[HapCUT2: robust and accurate haplotype assembly for diverse sequencing technologies]]></title>
	<description><![CDATA[HapCUT2 is a maximum-likelihood-based tool for assembling haplotypes from DNA sequence reads, designed to "just work" with excellent speed and accuracy. We found that previously described haplotype assembly methods are specialized for specific read technologies or protocols, with slow or inaccurate performance on others. With this in mind, HapCUT2 is designed for speed and accuracy across diverse sequencing technologies, including but not limited to:

NGS short reads (Illumina HiSeq)
clone-based sequencing (Fosmid or BAC clones)
SMRT reads (PacBio)
Oxford Nanopore reads
10X Genomics Linked-Reads
proximity-ligation (Hi-C) reads
high-coverage sequencing (&gt;40x coverage-per-SNP) using above technologies
combinations of the above technologies (e.g. scaffold long reads with Hi-C reads)
See below for specific examples of command line options and best practices for some of these technologies.

NOTE: At this time HapCUT2 is for diploid organisms only. VCF input should contain diploid variants.

If you use HapCUT2 in your research, please cite:

Edge, P., Bafna, V. &amp; Bansal, V. HapCUT2: robust and accurate haplotype assembly for diverse sequencing technologies. Genome Res. gr.213462.116 (2016). doi:10.1101/gr.213462.116<p>Address of the bookmark: <a href="https://github.com/vibansal/HapCUT2" rel="nofollow">https://github.com/vibansal/HapCUT2</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/28926/scientist-at-advanced-centre-for-treatment-research-and-education-in-cancer-navi-mumbai-maharashtra</guid>
  <pubDate>Tue, 30 Aug 2016 04:16:15 -0500</pubDate>
  <link></link>
  <title><![CDATA[Scientist at Advanced Centre for Treatment, Research and Education in Cancer - Navi Mumbai, Maharashtra]]></title>
  <description><![CDATA[
<p>Scientist <br />Advanced Centre for Treatment, Research and Education in Cancer - Navi Mumbai, Maharashtra<br />Scientist (One position) <br />Project: Bioinformatics centre DBT- Sub-DIC at ACTREC <br />Funding agency: DBT Grant No.232 </p>

<p>Duration of the Project: Six Months from the date of appointment can be extended further for six months <br />Essential Qualification and Experience: 1st Class Masters Degree in Bioinformatics or Life Sciences equivalent degree from a recognized University with 4 years R&amp;D experience in Bioinformatics or relevant subjects from recognized institutes. <br />OR <br />Ph.D. degree in Bioinformatics or Life Sciences from recognized University. <br />M.Sc. degree obtained after a one year course will not be considered. <br />Experience: Research/teaching experience in Bioinformatics or relevant subjects form recognized Institute(s). </p>

<p>More at http://www.actrec.gov.in/data%20files/Vacancies/2016/AV-scin-stud-trainee-6-Sept-16.docx</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37291/transrate-understanding-your-transcriptome-assembly</guid>
	<pubDate>Fri, 13 Jul 2018 07:49:26 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37291/transrate-understanding-your-transcriptome-assembly</link>
	<title><![CDATA[transrate: Understanding your transcriptome assembly]]></title>
	<description><![CDATA[<p><span>Transrate is software for&nbsp;</span><em>de-novo</em><span>&nbsp;transcriptome assembly quality analysis. It examines your assembly in detail and compares it to experimental evidence such as the sequencing reads, reporting quality scores for contigs and assemblies. This allows you to choose between assemblers and parameters, filter out the bad contigs from an assembly, and help decide when to stop trying to improve the assembly.</span></p><p>Address of the bookmark: <a href="http://hibberdlab.com/transrate/index.html" rel="nofollow">http://hibberdlab.com/transrate/index.html</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
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