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<channel>
	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/30976?offset=400</link>
	<atom:link href="https://bioinformaticsonline.com/related/30976?offset=400" rel="self" type="application/rss+xml" />
	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/39865/blast-nr-version-5-database-nr-v5</guid>
	<pubDate>Fri, 23 Aug 2019 11:35:35 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/39865/blast-nr-version-5-database-nr-v5</link>
	<title><![CDATA[BLAST nr version 5 database, (nr_v5)]]></title>
	<description><![CDATA[<p>NCBI have made changes the nr version 5 database, (nr_v5), to facilitate better search results and improved performance by reducing the number of redundant titles in the nr_v5 database used by webBLAST, which is also available for&nbsp;BLAST+ users.</p><p><span style="text-decoration: underline;"></span></p><p>The changes in nr preserve the taxonomic diversity of the entries in the database while reducing the number of titles for identical sequences. GenPept accessions are still accessible via&nbsp;<a href="http://www.ncbi.nlm.nih.gov/protein/$GENBANK_ACCESSION" target="_blank">www.ncbi.nlm.nih.gov/protein/$GENBANK_ACCESSION</a>&nbsp;or the IPG website&nbsp;<a href="https://www.ncbi.nlm.nih.gov/ipg/" target="_blank">https://www.ncbi.nlm.nih.gov/ipg/</a>.<span style="text-decoration: underline;"></span><span style="text-decoration: underline;"></span></p><p>The "Identical Proteins" link in the alignments section of the webBLAST results takes you to a full list of all accessions associated with a sequence.</p><p><span style="text-decoration: underline;"></span></p><p>For&nbsp;BLAST+ users downloading nr_v5: the database is now approximately 50% smaller, resulting in faster downloads and&nbsp;BLAST&nbsp;searches, and smaller disk space requirements. The database is downloadable at: &nbsp;<a href="ftp://ftp.ncbi.nlm.nih.gov/blast/db/v5/" target="_blank">ftp://ftp.ncbi.nlm.nih.gov/blast/db/v5/</a></p><p><span style="text-decoration: underline;"></span></p><p>For&nbsp;BLAST+ there is a cleanup script to help you manage the transition to this smaller database. The script removes unused database volumes:&nbsp;<a href="ftp://ftp.ncbi.nlm.nih.gov/blast/temp/cleanup-blastdb-volumes.py" target="_blank">ftp://ftp.ncbi.nlm.nih.gov/blast/temp/cleanup-blastdb-volumes.py</a></p><p><span style="text-decoration: underline;"></span></p><p>Here are the new rules on how we keep titles in nr_v5:</p><p><span style="text-decoration: underline;"></span></p><p>1.&nbsp;&nbsp;&nbsp; We keep all refseq, swissprot, pir and PDB titles.<span style="text-decoration: underline;"></span><span style="text-decoration: underline;"></span></p><p>2.&nbsp; &nbsp;&nbsp;We keep any GenPept titles with a TAXID that has not already been seen in the record.<span style="text-decoration: underline;"></span><span style="text-decoration: underline;"></span></p><p>3.&nbsp; &nbsp;&nbsp;We keep at least five GenPept titles regardless of whether the TAXIDS have been seen before or not in this record.</p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/4108/tijana-milenkovic-lab</guid>
  <pubDate>Fri, 30 Aug 2013 06:45:20 -0500</pubDate>
  <link></link>
  <title><![CDATA[Tijana Milenkovic Lab]]></title>
  <description><![CDATA[
<p>Complex networks and network mining: developing graph theoretic, mathematical, and computational algorithms for efficient extraction of function from topology of complex real-world networks, such as biological, social, and technological networks.</p>

<p>Computational and systems biology: studying the interplay between network topology and biological function, disease, and evolution in molecular (e.g., protein-protein interaction) networks.</p>

<p>Computational chemistry: protein folding; computational drug discovery and design.</p>

<p>Synthetic biology.</p>

<p>More at http://www.cse.nd.edu/~tmilenko/index.html</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/41586/primer-blast</guid>
	<pubDate>Tue, 28 Apr 2020 00:28:49 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/41586/primer-blast</link>
	<title><![CDATA[Primer BLAST !]]></title>
	<description><![CDATA[<p>BLAST team added a new feature (Max 3' match), shown in Figure 1, to Primer-BLAST that limits the length of 3' exon matches when designing exon-exon spanning primers. This makes it less likely that primers specifically designed to amplify transcripts will also amplify genomic DNA contamination in expression assays. See the NCBI Insights post (<a href="https://go.usa.gov/xvUT4" target="_blank"><span>https://go.usa.gov/xvUT4</span></a>) for more details.</p><p>&nbsp;</p><p><span>If you have any questions or concerns, please contact&nbsp;<a href="mailto:blast-help@ncbi.nlm.nih.gov" target="_blank" title="Follow link">blast-help@ncbi.nlm.nih.gov<sup><span><img src="https://mail.google.com/mail/u/0?ui=2&amp;ik=024a8aa0b9&amp;attid=0.1&amp;permmsgid=msg-f:1665129030912557674&amp;th=171bba0808bbc26a&amp;view=fimg&amp;sz=s0-l75-ft&amp;attbid=ANGjdJ-yC7WlxAuBOITc1ND1AN0YIdrtaQ3utEJuH_vnvOTM3uh8Wwn652wjlqDQ6HJOKApVPRJNpBRVd3H_AisXJXRWtzl0Y9alARMC05_yINEwa2lkBGoA7Q93-GU&amp;disp=emb" width="13" height="12" alt="image" style="border: 0px;"></span></sup></a></span></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/44515/cleaner-blast-databases-for-more-accurate-results</guid>
	<pubDate>Tue, 23 Apr 2024 01:23:08 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/44515/cleaner-blast-databases-for-more-accurate-results</link>
	<title><![CDATA[Cleaner BLAST Databases for More Accurate Results]]></title>
	<description><![CDATA[<p>Do you use&nbsp;<a href="https://blast.ncbi.nlm.nih.gov/Blast.cgi?utm_source=ncbi_insights&amp;utm_medium=referral&amp;utm_campaign=blast-cleaner-20240422">BLAST</a><span style="font-size: 12.8px; font-weight: normal;">&nbsp;to identify a sequence or the evolutionary scope of a gene? That can be challenging if contaminated and misclassified sequences are in the BLAST databases and show up in your search results. To address</span><span style="font-size: 12.8px; font-weight: normal;">&nbsp;this problem</span><span style="font-size: 12.8px; font-weight: normal;">, we now use the NCBI quality assurance tools listed below to systematically remove these misleading sequences from the default nucleotide (nt) and protein (nr) BLAST databases.</span><span style="font-size: 12.8px; font-weight: normal;">&nbsp;</span></p><div><ul>
<li><a href="https://github.com/ncbi/fcs">Foreign Contamination Screen tool for genome cross-species screening (FCS-GX)</a>&nbsp;detects contamination from foreign organisms in genomes and other sequences using the genome cross-species aligner (GX)&nbsp;</li>
<li><a href="https://ncbiinsights.ncbi.nlm.nih.gov/2022/05/27/ani-for-assembly-validation?utm_source=ncbi_insights&amp;utm_medium=referral&amp;utm_campaign=blast-cleaner-20240422">Average Nucleotide Identity (ANI)</a>&nbsp;evaluates the taxonomic classification of prokaryotic genome assemblies. Sequences from genomes marked up as &lsquo;unverified source organism&rsquo; are considered suspect and removed.&nbsp;</li>
</ul><p>Ref&nbsp;https://ncbiinsights.ncbi.nlm.nih.gov/2024/04/22/cleaner-blast-databases-more-accurate-results/</p></div>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>

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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/4352/jrf-bharathidasan-university</guid>
  <pubDate>Sat, 07 Sep 2013 14:20:03 -0500</pubDate>
  <link></link>
  <title><![CDATA[JRF @ BHARATHIDASAN UNIVERSITY]]></title>
  <description><![CDATA[
<p>Department of Bioinformatics<br />School of Life Sciences<br />BHARATHIDASAN UNIVERSITY,<br />TIRUCHIRAPPALLI-620 024</p>

<p>WALK-IN-INTERVIEW FOR JUNIOR RESEARCH FELLOWSHIP</p>

<p>Project title: Structural and Functional Evolution of Bacterial ADP-ribosylation Superfamily–A Special Emphasis for Engineering Immunotoxins from Binary toxin A Funding Agency: Life Science Research Board, Defence Research and Development Organization, New Delhi</p>

<p>Tenure of the project: Three years or till the end of the project period.</p>

<p>Position: Junior Research Fellow (1 no.)</p>

<p>Essential qualification: First class in M.Sc. in Genomics/Biotechnology/ Microbiology/ Biochemistry/Life Sciences</p>

<p>Desirable qualification: Experience in an area relevant (Molecular Microbiology, Protein engineering and Structural Bioinformatics) to the project.</p>

<p>Fellowship: Rs. 16, 000 per month plus HRA as per University rule.</p>

<p>Upper age limit: 28 years</p>

<p>Date of interview: 16-09-2013</p>

<p>Venue of interview: Department of Bioinformatics, Bharathidasan University, Tiruchirappalli -620 024, Tamil Nadu</p>

<p>The above post is purely temporary and will be terminated with three month notice. The Terms and the condition of the appointment shall be governed according to DRDO, Govt. of India. The eligible candidates will bring their original certificates and documents at the time of interview. No TA/DA will be paid for attending the interview.</p>

<p>Dr. P. CHELLAPANDI<br />Principal Investigator,<br />Department of Bioinformatics,<br />Bharathidasan University,<br />Tiruchirappalli -620 024, Tamil Nadu</p>

<p>Advertisement: http://www.bdu.ac.in/tender_list.php</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/37198/understanding-blastn-output-format-6</guid>
	<pubDate>Wed, 27 Jun 2018 18:38:21 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/37198/understanding-blastn-output-format-6</link>
	<title><![CDATA[Understanding BLASTn output format 6 !]]></title>
	<description><![CDATA[<h3 id="sites-page-title-header" style="text-align: left;"><span>BLASTn output format 6</span></h3><div id="sites-canvas-main"><div id="sites-canvas-main-content"><div dir="ltr"><div><div><em>BLASTn</em> maps DNA against DNA, for example gene sequences against a reference genome<br /><br /><code><strong>blastn</strong>  -query <span>genes.ffn</span>  -subject <span>genome.fna</span>  -outfmt <strong>6</strong></code></div><h2>BLASTn tabular output format 6</h2>
<p><strong>Column headers:</strong><br /><code>qseqid sseqid pident length mismatch gapopen qstart qend sstart send evalue bitscore</code><br /></p>
<table border="1" cellspacing="0">
<tbody>
<tr>
<td> 1.</td>
<td> qseqid</td>
<td> query (e.g., gene) sequence id</td>
</tr>
<tr>
<td> 2.</td>
<td> sseqid</td>
<td> subject (e.g., reference genome) sequence id</td>
</tr>
<tr>
<td> 3.</td>
<td> pident</td>
<td> percentage of identical matches</td>
</tr>
<tr>
<td> 4.</td>
<td> length</td>
<td> alignment length</td>
</tr>
<tr>
<td> 5.</td>
<td> mismatch</td>
<td> number of mismatches</td>
</tr>
<tr>
<td> 6.</td>
<td> gapopen</td>
<td> number of gap openings</td>
</tr>
<tr>
<td> 7.</td>
<td> qstart</td>
<td> start of alignment in query</td>
</tr>
<tr>
<td> 8.</td>
<td> qend</td>
<td> end of alignment in query</td>
</tr>
<tr>
<td> 9.</td>
<td> sstart</td>
<td> start of alignment in subject</td>
</tr>
<tr>
<td> 10.</td>
<td> send</td>
<td> end of alignment in subject</td>
</tr>
<tr>
<td> 11.</td>
<td> evalue</td>
<td> <a href="http://www.metagenomics.wiki/tools/blast/evalue">expect value</a></td>
</tr>
<tr>
<td> 12.</td>
<td> bitscore</td>
<td> <a href="http://www.metagenomics.wiki/tools/blast/evalue"><strong>bit score</strong></a></td>
</tr>
</tbody>
</table>
<p><strong><br /></strong></p>
</div><h2><a name="TOC-Define-your-own-output-format" id="TOC-Define-your-own-output-format"></a>Define your own output format</h2><div><em>by adding the option -outfmt, as for example: </em><strong><br /></strong></div>
<p><code><strong>-outfmt</strong> <strong>"6</strong> <span>qseqid sseqid pident qlen length mismatch gapope evalue bitscore</span><strong>"</strong></code><br /><br /><em><strong>supported format specifiers are:</strong></em><br /><code>qseqid    </code>Query Seq-id<br /><code>qgi       </code>Query GI<br /><code>qacc      </code>Query accesion<br /><code>qaccver   </code>Query accesion.version<br /><code>qlen      </code>Query sequence length<br /><code>sseqid    </code>Subject Seq-id<br /><code>sallseqid </code>All subject Seq-id(s), separated by a ';'<br /><code>sgi       </code>Subject GI<br /><code>sallgi    </code>All subject GIs<br /><code>sacc      </code>Subject accession<br /><code>saccver   </code>Subject accession.version<br /><code>sallacc   </code>All subject accessions<br /><code>slen      </code>Subject sequence length<br /><code>qstart    </code>Start of alignment in query<br /><code>qend      </code>End of alignment in query<br /><code>sstart    </code>Start of alignment in subject<br /><code>send      </code>End of alignment in subject<br /><code>qseq      </code>Aligned part of query sequence<br /><code>sseq      </code>Aligned part of subject sequence<br /><code>evalue    </code>Expect value<br /><code>bitscore  </code>Bit score<br /><code>score     </code>Raw score<br /><code>length    </code>Alignment length<br /><code>pident    </code>Percentage of identical matches<br /><code>nident    </code>Number of identical matches<br /><code>mismatch  </code>Number of mismatches<br /><code>positive  </code>Number of positive-scoring matches<br /><code>gapopen   </code>Number of gap openings<br /><code>gaps      </code>Total number of gaps<br /><code>ppos      </code>Percentage of positive-scoring matches<br /><code>frames    </code>Query and subject frames separated by a '/'<br /><code>qframe    </code>Query frame<br /><code>sframe    </code>Subject frame<br /><code>btop      </code>Blast traceback operations (BTOP)<br /><code>staxids   </code>Subject Taxonomy ID(s), separated by a ';'<br /><code>sscinames </code>Subject Scientific Name(s), separated by a ';'<br /><code>scomnames </code>Subject Common Name(s), separated by a ';'<br /><code>sblastnames </code>Subject Blast Name(s), separated by a ';'   (in alphabetical order)<br /><code>sskingdoms  </code>Subject Super Kingdom(s), separated by a ';'     (in alphabetical order) <br /><code>stitle      </code>Subject Title<br /><code>salltitles  </code>All Subject Title(s), separated by a '&lt;&gt;'<br /><code>sstrand   </code>Subject Strand<br /><code>qcovs     </code>Query Coverage Per Subject<br /><code>qcovhsp   </code>Query Coverage Per HSP<br /><strong><br /><em>default values are:</em></strong><br /><code><code>-outfmt "</code>6 qseqid sseqid pident length mismatch gapopen qstart qend sstart send evalue bitscore"</code></p>
</div></div></div>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/file/view/4482/bioinformatics-definitions-and-applications</guid>
	<pubDate>Thu, 12 Sep 2013 15:04:36 -0500</pubDate>
	<link>https://bioinformaticsonline.com/file/view/4482/bioinformatics-definitions-and-applications</link>
	<title><![CDATA[Bioinformatics definitions and applications !!!]]></title>
	<description><![CDATA[<p>There have been long discussion amongst several specialized/expert educator regarding bioinformatics arena, but everyone explain bioinformatics with their own view. I tried to explain it with a cartoon. Hope you all will like it.</p>]]></description>
	<dc:creator>Jit</dc:creator>
	<enclosure url="https://bioinformaticsonline.com/file/download/4482" length="49464" type="image/gif" />
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/43424/rest-api</guid>
	<pubDate>Mon, 04 Oct 2021 12:46:40 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/43424/rest-api</link>
	<title><![CDATA[REST API]]></title>
	<description><![CDATA[<h3 id="PSIBLASTHelpandDocumentation-RESTAPI">REST API</h3><p>The&nbsp;<a href="https://www.ebi.ac.uk/seqdb/confluence/pages/viewpage.action?pageId=68165098">Representational State Transfer (REST)</a>&nbsp;sample clients are provided for a number of programming languages. For details of how to use these clients,&nbsp;<a href="https://github.com/ebi-wp/webservice-clients">download</a>&nbsp;the client and run the program without any arguments.</p><div><table><colgroup><col><col><col></colgroup>
<thead>
<tr><th scope="col">
<div>Language</div>
</th><th scope="col">
<div>Download</div>
</th><th scope="col">
<div>Requirements</div>
</th></tr>
</thead>
<tbody>
<tr><th>Perl</th>
<td><a href="https://raw.githubusercontent.com/ebi-wp/webservice-clients/master/perl/psiblast.pl">psiblast.pl</a></td>
<td><a href="http://search.cpan.org/perldoc?LWP">LWP</a>&nbsp;and&nbsp;<a href="http://search.cpan.org/perldoc?XML::Simple">XML::Simple</a></td>
</tr>
<tr><th colspan="1">
<h4 id="PSIBLASTHelpandDocumentation-Python">Python</h4>
</th>
<td colspan="1">
<p><a href="https://raw.githubusercontent.com/ebi-wp/webservice-clients/master/python/psiblast.py">psiblast.py</a></p>
</td>
<td colspan="1"><a href="https://pypi.python.org/pypi/xmltramp2/3.0.10" title="https://pypi.python.org/pypi/xmltramp2/3.0.10">xmltramp2</a></td>
</tr>
</tbody>
</table></div><p>For details see&nbsp;<a href="https://www.ebi.ac.uk/seqdb/confluence/display/JDSAT/Environment+setup+for+REST+Web+Services">Environment setup for REST Web Services</a>&nbsp;and&nbsp;<a href="https://www.ebi.ac.uk/seqdb/confluence/display/JDSAT/Examples+for+Perl+REST+Web+Services+Clients">Examples for Perl REST Web Services Clients</a>&nbsp;pages.</p>]]></description>
	<dc:creator>Neel</dc:creator>
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<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/4575/hiv-phd-and-msc-research-positions</guid>
  <pubDate>Mon, 16 Sep 2013 18:55:44 -0500</pubDate>
  <link></link>
  <title><![CDATA[HIV PhD and MSc Research Positions]]></title>
  <description><![CDATA[
<p>SANBI are looking to recruit two MSc students and a PhD student who are interested in implementing a computational biology approach to explore HIV’s glycan shield. Successful candidates for the MSc should hold an honours degree in physics, computer science or biological sciences while PhD applicants should hold an honours degree and a MSc in one or more of physics, computer science or biological sciences.</p>

<p>As these positions are funded by the South African National Research Foundation (NRF) priority will be given to South African citizens and permanent residents however exceptional applicants who do not fulfil these criteria may be considered.</p>

<p>Applications including a CV outlining your experience together with a cover letter detailing why you are a suitable candidate should be sent to simon_at_sanbi.ac.za by 30th September 2013.</p>

<p>More @ http://www.sanbi.ac.za/hiv-phd-and-msc-research-positions/</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38449/koala-keggs-internal-annotation-tool-for-k-number-assignment-of-kegg-genes-using-ssearch-computation</guid>
	<pubDate>Wed, 12 Dec 2018 09:16:55 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38449/koala-keggs-internal-annotation-tool-for-k-number-assignment-of-kegg-genes-using-ssearch-computation</link>
	<title><![CDATA[KOALA: KEGG&#039;s internal annotation tool for K number assignment of KEGG GENES using SSEARCH computation]]></title>
	<description><![CDATA[<p>KOALA (KEGG Orthology And Links Annotation) is KEGG's internal annotation tool for&nbsp;<a href="https://www.kegg.jp/kegg/ko.html">K number</a>&nbsp;assignment of KEGG GENES using SSEARCH computation. BlastKOALA and GhostKOALA assign K numbers to the user's sequence data by&nbsp;<a href="http://www.ncbi.nlm.nih.gov/blast/">BLAST</a>&nbsp;and&nbsp;<a href="http://www.bi.cs.titech.ac.jp/ghostx/">GHOSTX</a>&nbsp;searches, respectively, against a nonredundant set of KEGG GENES. Annotate Sequence in KEGG Mapper and Pathogen Checker in KEGG Pathogen are special interfaces to the BlastKOALA server and can be executed in an interactive mode. &nbsp;&nbsp; See&nbsp;<a href="https://www.kegg.jp/blastkoala/help_blastkoala.html" target="_blastkoala">Step-by-step Instructions</a>.</p>
<div>Reference: Kanehisa, M., Sato, Y., and Morishima, K. (2016) BlastKOALA and GhostKOALA: KEGG tools for functional characterization of genome and metagenome sequences. J. Mol. Biol. 428, 726-731. [<a href="http://www.ncbi.nlm.nih.gov/pubmed/26585406">pubmed</a>] [<a href="https://doi.org/10.1016/j.jmb.2015.11.006">pdf</a>]</div><p>Address of the bookmark: <a href="https://www.kegg.jp/blastkoala/" rel="nofollow">https://www.kegg.jp/blastkoala/</a></p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
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