BOL: Related items

pWhatsHap: a parallel, high-performance version of WhatsHap

Jit — Wed, 14 Nov 2018 08:20:27 -0600

Given the potential relevance of efficient haplotyping in several analysis pipelines, we have designed and engineered pWhatsHap, a parallel, high-performance version of WhatsHap. pWhatsHap is embedded in a toolkit developed in Python and supports genomics datasets in standard file formats. Building on WhatsHap, pWhatsHap exhibits the same complexity exploring a number of possible solutions which is exponential in the coverage of the dataset. The parallel implementation on multi-core architectures allows for a relevant reduction of the execution time for haplotyping, while the provided results enjoy the same high accuracy as that provided by WhatsHap, which increases with coverage.

https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-016-1170-y

Address of the bookmark: https://bitbucket.org/whatshap/whatshap

Linux operating system aimed at scientists

Pranjali Yadav — Mon, 19 Jan 2015 08:30:49 -0600

The Bio-Linux operating system is based on Ubuntu 14.04 LTS (Trusty Tahr), and the previous version was using Ubuntu 12.04 LTS. The developers only use LTS releases and that means that upgrades for this distro don't come along all that often.

This Linux distribution is aimed at scientists and it comes with more than 250 bioinformatics packages, 50 graphical applications and several hundred command line tools. And this is just skimming the surface of what the OS can do. Users have access to even more apps from the official repositories.

Bio-Linux is using an Ubuntu LTS version as its base

The fact that it uses Ubuntu LTS versions for the base is a good thing because it means its users won't have to worry about the support. Ubuntu 14.04 LTS is supported until 2019, so people who are using Bio-Linux shouldn't have a problem.

"An updated Bio-Linux 8 version is now on the website in ISO and OVA versions. As usual, there is no need to download this version if you are an existing user. All updates to existing packages will be applied to your system through the update manager and new packages are all available via apt-get or Synaptic," reads the announcement.

The changelog also states that a problem that was preventing the desktop to not start on VirtualBox has been fixed, the QIIME and Bowtie-Bio tools have been upgraded, the pandaseq paired end assembler has been added, and the beginners tutorial specific to Bio-Linux 8 has been improved.

Check out the official announcement for a complete list of changes and updates. You can download Bio-Linux 8.0.5 right now from Softpedia and give it a spin. It has the Unity desktop and now it runs very well in virtual environments.

Reference @ http://news.softpedia.com/news/Bioinformatics-Distro-Bio-Linux-8-0-5-Now-Available-for-Download-469867.shtml

Genome Annotation using MAKER tutorial !

Shruti Paniwala — Thu, 20 Dec 2018 17:39:23 -0600

MAKER is a great tool for annotating a reference genome using empirical and ab initiogene predictions. GMOD, the umbrella organization that includes MAKER, has some nice tutorials online for running MAKER. However, these were quite simplified examples and it took a bit of effort to wrap my head completely around everything. Here I will describe a de novo genome annotation for Boa constrictor in detail, so that there is a record and that it is easy to use this as a guide to annotate any genome.

Address of the bookmark: https://www.biostars.org/p/261203/

DNA Transcription (Advanced)

Thu, 29 Jan 2015 05:31:42 -0600

Transcription is the process by which the information in DNA is copied into messenger RNA (mRNA) for protein production. Originally created for DNA Interactive ( http://www.dnai.org ). TRANSCRIPT: The Central Dogma of Molecular Biology: "DNA makes RNA makes protein" Here the process begins. Transcription factors assemble at a specific promoter region along the DNA. The length of DNA following the promoter is a gene and it contains the recipe for a protein. A mediator protein complex arrives carrying the enzyme RNA polymerase. It manoeuvres the RNA polymerase into place... inserting it with the help of other factors between the strands of the DNA double helix. The assembled collection of all these factors is referred to as the transcription initiation complex... and now it is ready to be activated. The initiation complex requires contact with activator proteins, which bind to specific sequences of DNA known as enhancer regions. These regions may be thousands of base pairs distant from the start of the gene. Contact between the activator proteins and the initiation-complex releases the copying mechanism. The RNA polymerase unzips a small portion of the DNA helix exposing the bases on each strand. Only one of the strands is copied. It acts as a template for the synthesis of an RNA molecule which is assembled one sub-unit at a time by matching the DNA letter code on the template strand. The sub-units can be seen here entering the enzyme through its intake hole and they are joined together to form the long messenger RNA chain snaking out of the top.

Genome assembly forensics: finding the elusive mis-assembly

Jit — Sat, 26 Jan 2019 18:02:01 -0600

We present the first collection of tools aimed at automated genome assembly validation. This work formalizes several mechanisms for detecting mis-assemblies, and describes their implementation in our automated validation pipeline, called amosvalidate. We demonstrate the application of our pipeline in both bacterial and eukaryotic genome assemblies, and highlight several assembly errors in both draft and finished genomes. The software described is compatible with common assembly formats and is released, open-source, at http://amos.sourceforge.net.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2397507/

http://amos.sourceforge.net/wiki/index.php/AMOS

Address of the bookmark: http://amos.sourceforge.net/wiki/index.php/AMOS

RA Bioinformatics at CIBA

Mon, 02 Feb 2015 22:52:50 -0600

The following posts are to be filled purely on temporary basis under CIBA Component of “Centre for Agricultural Bioinformatics (CABin)” project at this Institute.

Research Associate – one post

Date & Time of Interview 18th Feb 2015 at 10.00 a.m.

Essential Qualification Ph.d / M.Sc./ M.Phil (Bioinformatics) With 1st division or 60% marks or equivalent overall grade point average with at least two years of research experience in the relevant subject.

Desirable qualification: Experience in Java/ C++/ PHP/ PERL/ Python etc. based application development using Linux, Apache and MySQL/Oracle.

Emoluments Rs.24000/- p.m. + 30% HRA for Ph.D holders / Rs.23000/- p.m. + 30% HRA for Master Degree holders A consolidated pay Rs.25000/- per month.

Age Limit Maximum 40 years for men and 45 years for women as on date of interview. Age limits are relaxable for SC / ST / OBC candidates as per rules. Maximum 40 years for men and 45 years for women as on date of interview. Age limits are relaxable for SC / ST / OBC candidates as per rules.

Eligible Candidates may appear for the Walk-in-interview with original Certificate of Ph.D. / Master’s / relevant degree, passport size photograph and bio-data enclosing attested copies of educational qualification & experience certificates. TA / DA will not be paid for attending the interview.

Advertisement: www.ciba.res.in/attachments/jobs/CABin-3006.pdf

gEVAL: Genome Evaluation Browser

Jit — Tue, 18 Jun 2019 05:39:08 -0500

The gEVAL Browser allows the evaluation of genome assemblies through its tools and pre-computed analyses.

The strength of this browser is the ability to navigate an up to date assembly and identify problematic regions and assist in strategizing potential solutions for these issues.

This facilitates the improvement of overall assemblies to a “gold” standard for release as reference genomes

Address of the bookmark: https://geval.sanger.ac.uk/index.html

JRF in Bioinformatics @ Tezpur University

Mon, 02 Feb 2015 22:50:14 -0600

Applications are invited for one temporary position of Junior Research Fellow (JRF) in the DBT Twinning Project entitled ‘In-Silico design and evaluation of sequences for γD crystalline protein’ under the Principal Investigator Dr. Anupam Nath Jha, Assistant Professor, Department of Molecular Biology and Biotechnology, Tezpur University.

Educational Qualification: Candidate must possess a M.Sc. in Bioinformatics/ Biotechnology/ Computer Science/ Physics/ Chemistry or B.Tech. or M.Tech. in Bioinformatics/ Biotechnology/ Computer Science from a recognized University/ Institute with minimum 60% marks or equivalent CGPA for general category and for SC/ST/OBC relaxation will be given as per Govt. of India rules.

Fellowship: Rs 16,000/- (Rupees sixteen thousand) only + 10% HRA per month for NET/GATE/BET/BINC qualified candidates. Rs. 12,000/- (Rupees twelve thousand) only + 10% HRA per month for other candidates.

Duration: Initially for a period of six (06) months which may be extended depending upon status of the project or until further order, whichever is earlier.

Age: Candidate should not be more than 28 years of age on the date of interview. Upper age limit may be relaxed up to 5 years in the case of candidate belonging to SC/ ST /OBC /Women / Physically Challenged.

Interested candidates may send their application on plain paper by post along with his/her educational qualifications, recent passport/stamp size photograph and contact phone number to Dr. Anupam Nath Jha, Principal Investigator, Department of Molecular Biology and Biotechnology, Tezpur University, Napaam – 784028 or mail it to anjha@tezu.ernet.in within 20 days of publication of this advertisement.

Only shortlisted candidates will be informed by e-mail or phone for an interview. N.B. No TA/DA will be paid for attending the interview. For further details contact: Dr. Anupam Nath Jha, Assistant Professor, Principal Investigator Department of Molecular Biology & Biotechnology Tezpur University, Napaam, Tezpur-784028 (Assam)

Advertisement: www.tezu.ernet.in/ProjectWalkin/Advt-ANJ-5323-A.pdf

De novo Genome Assembly for Illumina Data

Rahul Nayak — Mon, 20 Jan 2020 05:13:29 -0600

Written and maintained by Simon Gladman - Melbourne Bioinformatics (formerly VLSCI)

Protocol Overview / Introduction

In this protocol we discuss and outline the process of de novo assembly for small to medium sized genomes.

https://www.melbournebioinformatics.org.au/tutorials/tutorials/assembly/assembly-protocol/

Address of the bookmark: https://www.melbournebioinformatics.org.au/tutorials/tutorials/assembly/assembly-protocol/

RA / Research Associate Bioinformatics/Computational Biologist

Thu, 26 Feb 2015 03:44:24 -0600

The following posts are urgently required to be filled for the Department of Biotechnology, Government of India funded project entitled "Computational Core for Plant Metabolomics" administrated by Prof Indira Ghosh, School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi-110 067

RA / Research Associate (Bioinformatics/Computational Biologist)
Salary: Rs. 23000/- + HRA
Vacancy: 1
Essential Qualifications: PhD, preferably in plant biotechnology/biochemistry/Bioinformatics with experience in analyzing high throughput omics-based data. Desired Skills: Prior experience in handling and guiding bioinformatics, metabolomics data, managing the project portal, preparing and filing reports etc. Will be expected to communicate with user groups and coordinate with LIMS group in Hyderabad and the Cheminformatics group in Delhi.

Address: Prof Indira Ghosh, room #12 Project Investigator, (Core Metabolomics) School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi-110 067.

Apply Details
Last Date: March 3rd 2015
Web/Notification URL http://www.jnu.ac.in/Career/currentjobs. htm