BOL: Related items

Peregrine & SHIMMER Genome Assembly Toolkit

Abhi — Thu, 16 Dec 2021 02:50:19 -0600

Peregrine is a fast genome assembler for accurate long reads (length > 10kb, accuracy > 99%). It can assemble a human genome from 30x reads within 20 cpu hours from reads to polished consensus. It uses Sparse HIereachical MimiMizER (SHIMMER) for fast read-to-read overlaping without quadratic comparisions used in other OLC assemblers.

Address of the bookmark: https://github.com/cschin/Peregrine

Comparative Genomics Workshops !

Rahul Nayak — Tue, 25 Jan 2022 20:39:58 -0600

This meeting's objective was to obtain a big picture look at the current state of the field of comparative genomics with a focus on commonalities across genomic investigations into humans, model organisms (both traditional and non-traditional), agricultural species, wildlife species and microbes.

https://www.genome.gov/event-calendar/perspectives-in-comparative-genomics-and-evolution

Address of the bookmark: https://www.genome.gov/event-calendar/perspectives-in-comparative-genomics-and-evolution

NGS Online Training

Sat, 27 Sep 2014 07:42:29 -0500

ArrayGen Technologies announces to provide online NGS training through out the globe. Now analyze your own NGS datasets from anywhere.For more information contact us at training@arraygen.com

Please visit our site at www.arraygen.com

ALE: Assembly Likelihood Estimator

BioStar — Wed, 08 Mar 2023 01:39:33 -0600

Just import the assembly, bam and ALE scores. You can convert the .ale file to a set of .wig files with ale2wiggle.py and IGV can read those directly. Depending on your genome size you may want to convert the .wig files to the BigWig format.

Address of the bookmark: https://github.com/sc932/ALE

JRF in Bioinformatics @ INMAS, DRDO,Delhi

Wed, 01 Oct 2014 07:01:07 -0500

Institute of Nuclear Medicine and Allied Sciences (INMAS), Delhi under the aegis of Defence Research and Development Organisation (DRDO), is engaged in research and developmental work in radiation sciences, Neuro-Computing and Medical Image Processing. INMAS is looking for meritorious young researchers for pursuing research in the frontier areas at INMAS. The Institute invites applications from young and meritorious Indian nationals who are creative, have passion and desire to pursue R&D in frontier areas. INMAS possesses ambience of a research cum academic institute coupled with an advanced R&D infrastructure in a mission mode. It provides the best infrastructure, motivation and personality development prospects for talented students, dreaming of unparalleled success in their professional endeavors. INMAS provides state of the art research facilities for undertaking pioneering research with defence applications.

JRF (Maximum Tenure‐ Five Years: 2yrs as JRF and 3yrs as SRF)
A first class Master’s Degree in Bioinformatics (likely 2 posts)
Around Rs 16,000/ Plus 30% HRA (as per rules of funding agency)

Applications are invited from candidates possessing the above qualifications. The upper age limit is as on the last date for receipt of application. (5 years relaxation to SC/ST candidates, 3 years to OBC candidates, and other entitled categories as per Govt rules). Actual No. of vacancies may vary.

Application form can be download from the website www.drdo.gov.in and E Mailed to inmashrd@gmail.com.
Last date to apply by email is 1700 hrs on 15 Oct 2014
Incomplete applications are liable to be rejected.
Confirmation will be sent to short-listed candidates through email only
Antecedents of selected candidates will be verified.
Written Test will be conducted from 0930-1030 hrs. Latecomers will not be considered.
Candidates will be required to produce certificates/testimonials in original at the time of interview.
It may please be noted that offer of Fellowship does not confer on fellows any right for absorption in DRDO.
Candidates should carry photocopy of Application form sent by email with them.
No TA/DA will be paid for attending interview & on joining.
Last date to apply by email is 1700 hrs on 15 Oct 2014

More at http://drdo.gov.in/drdo/English/jrf29092014.pdf
http://drdo.gov.in/drdo/English/index.jsp?pg=inmas29092014.jsp

Steps to find all the repeats in the genome !

Neel — Thu, 31 Aug 2023 02:43:28 -0500

To find repeats in a genome from 2 to 9 length using a Perl script, you can use the RepeatMasker tool with the "--length" option[0]. Here's a step-by-step guide:

Install RepeatMasker: First, you need to install RepeatMasker on your system. You can download it from the RepeatMasker website[0].

Prepare the genome sequence: Make sure you have the genome sequence in a FASTA file format. Let's assume the file is named "genome.fasta".

./RepeatMasker -pa -nolow -norna -no_is -div -lib RepeatMaskerLib.embl -gff -xsmall -small -poly -species -dir -length - genome.fasta

Replace the following placeholders with appropriate values:

: The number of processors/threads you want to use for parallel processing.
: The divergence value for the species you are analyzing. You can find divergence values for different species in the RepeatMasker documentation[0].
: The name of the species you are analyzing.
: The directory where you want the output files to be saved.
and : The minimum and maximum lengths of the repeats you want to find (in this case, 2 and 9).

Analyze the output: RepeatMasker will generate several output files, including a .out file. You can parse this file to extract the information you need. There is a Perl tool called "one_code_to_find_them_all.pl" that can help you parse RepeatMasker output files[0]. You can download it from the source provided.

Use the provided Perl script: Once you have the "one_code_to_find_them_all.pl" script, you can run it to conveniently parse the RepeatMasker output files. Here's an example of how to use it:

perl one_code_to_find_them_all.pl --rm --length

Replace with the path to your RepeatMasker .out file, and with the path to a file containing the lengths of the reference elements.

This script will generate several output files, including .log.txt and .copynumber.csv, which contain quantitative information about the identified repeat elements.

Remember to adjust the parameters and options according to your specific needs and the characteristics of your genome.

Internship Program for Bioinformatics / Biotechnology Professionals (No. Of Vacancy: 2)

Wed, 08 Oct 2014 01:10:08 -0500

ArrayGen is offering an Internship Program for Post graduate Bioinformatics / Biotechnology students and professionals. ArrayGen Technologies provide an excellent opportunity to gain research experience and explore if a scientific career is right for you. Currently we offer positions to outstanding students interested in Next Generation Sequencing (NGS) data analysis. Applications are accepted throughout the year. Accepted students will be listed on web with their schedules. Accepted students can attend our future workshops and trainings freely at the specified venue.

UnCoVar: Workflow for Transparent and Robust Virus Variant Calling, Genome Reconstruction and Lineage Assignment

BioStar — Mon, 05 Aug 2024 23:01:29 -0500

UnCoVar: Workflow for Transparent and Robust Virus Variant Calling, Genome Reconstruction and Lineage Assignment

Using state of the art tools, easily extended for other viruses
Tool and database updates for critical components via Conda
Built using modern design patterns with Conda and Snakemake
Extensible and easy to customize
Submission Ready Genomes
Customizable reporting with comprehensive visualization

https://ikim-essen.github.io/uncovar/

Github https://github.com/IKIM-Essen/uncovar

Address of the bookmark: https://ikim-essen.github.io/uncovar/

How far can bioinformatics go creating organisms used for testing?

Fri, 17 Oct 2014 02:08:16 -0500

"I think you can get very far on a technical level. The problem is that a human body is more complex than just one cell." ... "At some point we still need clinical tests on animals and humans before we use it for real treatment. But we will likely be able to remove 99 % of animal tests in the future." Erik Lindahl, Professor of Theoretical and Computational Biophysics at KTH Royal Institute of Technology is telling us about his work. From the episode "Science for life – mapping the building blocks of the human body". Watch the rest of the talk, and other talks at www.crosstalks.tv Crosstalks is an academic talkshow produced by KTH Royal Institute of Technology and Stockholm University.

NVIDIA and Arc Institute Unveil Evo 2: A Breakthrough AI for DNA Design

BioStar — Fri, 21 Feb 2025 10:39:47 -0600

NVIDIA and the Arc Institute have introduced Evo 2, a groundbreaking AI model designed to understand, predict, and generate DNA sequences. This marks a major advancement in computational biology, offering scientists an unprecedented tool to decode the genetic blueprint of life and even design entirely new biological systems.

The Power of Evo 2: AI Meets DNA

Evo 2 is the largest AI model for biology ever created, trained on an astonishing 9.3 trillion DNA "letters" (nucleotides) carefully selected from genomes spanning the entire tree of life. This massive dataset ensures that Evo 2 can recognize patterns and relationships in genetic sequences at an unparalleled scale.

For the first time, scientists can design DNA with AI, moving beyond simple sequence analysis to active DNA generation. Evo 2 enables researchers to predict, modify, and even create entire genetic sequences, opening new possibilities in medicine, agriculture, and synthetic biology.

Decoding the Dark Genome

One of the biggest challenges in genetics is understanding the non-coding regions of DNA—vast stretches of the genome that do not code for proteins but play crucial roles in regulating gene expression. These regions control when and how genes are activated, influencing everything from development to disease.

Evo 2 is designed to decode these non-coding elements, helping researchers uncover their functions and use this knowledge to develop gene-based therapies, synthetic life forms, and precision agriculture solutions.

From Reading DNA to Writing It

To put Evo 2’s impact into perspective:

Previous AI models could "read" DNA like a book, analyzing genetic sequences and identifying patterns.
Evo 2 can "write" entirely new DNA, designing functional genes, chromosomes, and even full genomes from scratch.

This means scientists can now engineer biological systems with AI, designing new proteins, metabolic pathways, and genetic circuits to address real-world challenges.

A Step Toward Generative Biology

The Arc Institute describes Evo 2 as a major step toward "generative biology"—a revolutionary approach where AI is used to create novel biological structures rather than just analyzing existing ones. This could lead to breakthroughs such as:

New medicines: AI-generated enzymes and proteins tailored for targeted therapies.
Disease-resistant crops: Genetically optimized plants for higher yield and climate resilience.
Synthetic organisms: Custom-designed microbes for bioremediation, biofuel production, and industrial applications.

An Open-Source Revolution

Unlike many proprietary AI models, Evo 2 is open source, making its capabilities accessible to researchers worldwide. This democratization of AI-driven biology means that scientists from different disciplines can collaborate, experiment, and innovate, accelerating discoveries in genetic engineering and synthetic biology.

With Evo 2, the boundaries of what’s possible in DNA design, genetic engineering, and biological innovation are being redrawn. The future of life sciences is no longer just about understanding life’s code—it’s about writing it.