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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/31345?offset=910</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43254/quasr-quantification-and-annotation-of-short-reads-in-r</guid>
	<pubDate>Fri, 13 Aug 2021 07:44:05 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43254/quasr-quantification-and-annotation-of-short-reads-in-r</link>
	<title><![CDATA[QuasR: Quantification and annotation of short reads in R]]></title>
	<description><![CDATA[<p>The <em><a href="https://bioconductor.org/packages/3.14/QuasR">QuasR</a></em> package (short for <em>Qu</em>antify and <em>a</em>nnotate <em>s</em>hort reads in <em>R</em>) integrates the functionality of several <strong>R</strong> packages (such as <em><a href="https://bioconductor.org/packages/3.14/IRanges">IRanges</a></em> <span>(Lawrence et al. 2013)</span> and <em><a href="https://bioconductor.org/packages/3.14/Rsamtools">Rsamtools</a></em>) and external software (e.g.&nbsp;<code>bowtie</code>, through the <em><a href="https://bioconductor.org/packages/3.14/Rbowtie">Rbowtie</a></em> package, and <code>HISAT2</code>, through the <em><a href="https://bioconductor.org/packages/3.14/Rhisat2">Rhisat2</a></em> package). The package aims to cover the whole analysis workflow of typical high throughput sequencing experiments, starting from the raw sequence reads, over pre-processing and alignment, up to quantification. A single <strong>R</strong> script can contain all steps of a complete analysis, making it simple to document, reproduce or share the workflow containing all relevant details.</p><p>Address of the bookmark: <a href="https://www.bioconductor.org/packages/devel/bioc/vignettes/QuasR/inst/doc/QuasR.html" rel="nofollow">https://www.bioconductor.org/packages/devel/bioc/vignettes/QuasR/inst/doc/QuasR.html</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/30698/itol-interactive-tree-of-life</guid>
	<pubDate>Tue, 31 Jan 2017 05:56:30 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/30698/itol-interactive-tree-of-life</link>
	<title><![CDATA[iTOL: interactive Tree Of Life]]></title>
	<description><![CDATA[<p><strong>Interactive Tree Of Life</strong><span>&nbsp;is an online tool for the display and manipulation of phylogenetic trees. It provides most of the features available in other tree viewers, and offers a novel circular tree layout, which makes it easy to visualize mid-sized tree (up to several thousand leaves). Trees can be exported to several graphical formats, both bitmap and vector based.</span></p>
<p><img src="http://itol.embl.de/img/home/ex3.png" alt="image" style="border: 0px;"><br><span>There are several pre-computed trees available for display, including the main Tree Of Life, described in&nbsp;</span><a href="http://www.ncbi.nlm.nih.gov/pubmed/16513982">Ciccarelli, et al., 2006</a><span>. In addition to the precomputed trees, users can upload and display personal trees and data, using the 'Data upload' page or through a personal user account.</span></p><p>Address of the bookmark: <a href="http://itol.embl.de/" rel="nofollow">http://itol.embl.de/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/33219/dbcan-a-web-server-and-database-for-automated-carbohydrate-active-enzyme-annotation</guid>
	<pubDate>Mon, 29 May 2017 05:39:29 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/33219/dbcan-a-web-server-and-database-for-automated-carbohydrate-active-enzyme-annotation</link>
	<title><![CDATA[dbCAN: a web server and DataBase for automated Carbohydrate-active enzyme ANnotation]]></title>
	<description><![CDATA[<p><a href="http://csbl.bmb.uga.edu/dbCAN/index.php">dbCAN</a>&nbsp;is a web server and&nbsp;<span style="text-decoration: underline;">D</span>ata<span style="text-decoration: underline;">B</span>ase for&nbsp;<a href="http://csbl.bmb.uga.edu/dbCAN/annotate.php"><strong>automated&nbsp;<span style="text-decoration: underline;">C</span>arbohydrate-active enzyme&nbsp;<span style="text-decoration: underline;">AN</span>notation</strong></a>, funded by the&nbsp;<a href="http://bioenergycenter.org/">BioEnergy Science Center of the DOE</a>. Similar resources on the web include&nbsp;<a href="http://www.cazy.org/" target="_blank">CAZy database</a>&nbsp;and&nbsp;<a href="http://cricket.ornl.gov/cgi-bin/cat.cgi" target="_blank">CAT</a>. All data in dbCAN are generated based on the family classification from&nbsp;<a href="http://www.cazy.org/" target="_blank">CAZy database</a>&nbsp;while it has the following&nbsp;<strong><span style="text-decoration: underline;">unique features</span></strong>&nbsp;compared with CAZy database and CAT:</p>
<ul>
<li>dbCAN provides the capability of&nbsp;<a href="http://csbl.bmb.uga.edu/dbCAN/annotate.php">automated and comprehensive CAZyme annotation</a>&nbsp;of a given genome submitted by the user;</li>
<li>dbCAN provides an explicitly defined&nbsp;<span style="text-decoration: underline;">signature domain</span>&nbsp;for each and every CAZyme family along with its location in all the relevant full-length CAZyme proteins in all sequenced&nbsp;<a href="http://csbl.bmb.uga.edu/dbCAN/genome.php">genomes</a>;</li>
<li>dbCAN provides the most complete set of&nbsp;<span style="text-decoration: underline;">metagenomic CAZyme</span>&nbsp;genes published so far and represents the first step towards discovering novel CAZyme catalysts in metagenomes;</li>
<li>dbCAN provides a&nbsp;<span style="text-decoration: underline;">subfamily classification</span>&nbsp;of the existing CAZyme families based on sequence similarities;</li>
<li>dbCAN make all pre-computed data freely available to the public, including sequence alignments,&nbsp;<a href="http://csbl.bmb.uga.edu/dbCAN/download/">hidden markov models (HMMs)</a>&nbsp;and phylogenies of the signature domain regions in each and every CAZyme family and subfamily.</li>
</ul>
<p><a href="http://csbl.bmb.uga.edu/dbCAN/help.php">dbCAN</a>&nbsp;is updated regularly when&nbsp;<a href="http://www.cazy.org/" target="_blank">CAZy database</a>&nbsp;created new families based on latest literature.</p><p>Address of the bookmark: <a href="http://csbl.bmb.uga.edu/dbCAN/index.php" rel="nofollow">http://csbl.bmb.uga.edu/dbCAN/index.php</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/30831/fsa-fast-statistical-alignment</guid>
	<pubDate>Mon, 06 Feb 2017 04:26:01 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/30831/fsa-fast-statistical-alignment</link>
	<title><![CDATA[FSA: Fast Statistical Alignment]]></title>
	<description><![CDATA[<p><span>FSA is a probabilistic multiple sequence alignment algorithm which uses a "distance-based" approach to aligning homologous protein, RNA or DNA sequences. Much as distance-based phylogenetic reconstruction methods like Neighbor-Joining build a phylogeny using only pairwise divergence estimates, FSA builds a multiple alignment using only pairwise estimations of homology. This is made possible by the sequence annealing technique for constructing a multiple alignment from pairwise comparisons, developed by Ariel Schwartz in&nbsp;</span><a href="http://www.eecs.berkeley.edu/Pubs/TechRpts/2007/EECS-2007-39.html">"Posterior Decoding Methods for Optimization and Control of Multiple Alignments</a><span>."</span></p>
<p>FSA brings the high accuracies previously available only for small-scale analyses of proteins or RNAs to large-scale problems such as aligning thousands of sequences or megabase-long sequences. FSA introduces several novel methods for constructing better alignments:</p>
<ul>
<li>FSA uses machine-learning techniques to estimate gap and substitution parameters on the fly for each set of input sequences. This "query-specific learning" alignment method makes FSA very robust: it can produce superior alignments of sets of homologous sequences which are subject to very different evolutionary constraints.</li>
<li>FSA is capable of aligning hundreds or even thousands of sequences using a randomized inference algorithm to reduce the computational cost of multiple alignment. This randomized inference can be over ten times faster than a direct approach with little loss of accuracy.</li>
<li>FSA can quickly align very long sequences using the "anchor annealing" technique for resolving anchors and projecting them with transitive anchoring. It then stitches together the alignment between the anchors using the methods described above.</li>
<li>The included GUI, MAD (Multiple Alignment Display), can display the intermediate alignments produced by FSA, where each character is colored according to the probability that it is correctly aligned (see the picture and&nbsp;<a href="http://fsa.sourceforge.net/images/Suchard_SIV.fsa.mov">movie</a>&nbsp;at the top of the page).</li>
</ul>
<p><span>You can see more information on the&nbsp;</span><a href="http://fsa.sourceforge.net/FAQ.html">FAQ</a><span>.&nbsp;</span></p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="http://fsa.sourceforge.net/" rel="nofollow">http://fsa.sourceforge.net/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34398/ont-assembly-and-illumina-polishing-pipeline</guid>
	<pubDate>Thu, 23 Nov 2017 10:13:42 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34398/ont-assembly-and-illumina-polishing-pipeline</link>
	<title><![CDATA[ONT assembly and Illumina polishing pipeline]]></title>
	<description><![CDATA[<p>This pipeline performs the following steps:</p>
<ul>
<li>Assembly of nanopore reads using&nbsp;<a href="http://canu.readthedocs.io/">Canu</a>.</li>
<li>Polish canu contigs using&nbsp;<a href="https://github.com/isovic/racon">racon</a>&nbsp;(<em>optional</em>).</li>
<li>Map a paired-end Illumina dataset onto the contigs obtained in the previous steps using&nbsp;<a href="http://bio-bwa.sourceforge.net/">BWA</a>&nbsp;mem.</li>
<li>Perform correction of contigs using&nbsp;<a href="https://github.com/broadinstitute/pilon/wiki">pilon</a>&nbsp;and the Illumina dataset.</li>
</ul><p>Address of the bookmark: <a href="https://github.com/nanoporetech/ont-assembly-polish" rel="nofollow">https://github.com/nanoporetech/ont-assembly-polish</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/30901/ideoplot</guid>
	<pubDate>Mon, 13 Feb 2017 09:47:32 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/30901/ideoplot</link>
	<title><![CDATA[Ideoplot]]></title>
	<description><![CDATA[<p>Simple ideogram plotting and annotation in R.</p>
<p>Basic usage:</p>
<p>Rscript Ideoplot.R --heatmap hm.bed --annotate annotations.bed --out ideogram.pdf<br> -or-<br> Rscript Ideoplot.R --annotate annotations.bed</p>
<pre>Options
  --ideobed, i      A bed file of reference contig lengths/chromosome names
  --heatmap, -h     Fill chromosomes with normalized heatmap
                   (described below)
  --annotate, -a    Add character annotations.
  --out, -o         PDF output name.
  --stripes, -s     Specify a file containing the layout of the
                    annotations (description below)
  --bars, -b        Add track annotations
  --reference, -f   Either hg19, or hg38
  --topdown, r      Flag, when set, flips the orientation (P arms
                    drawn on top).
</pre><p>Address of the bookmark: <a href="https://github.com/mchaisso/Ideoplot" rel="nofollow">https://github.com/mchaisso/Ideoplot</a></p>]]></description>
	<dc:creator>Shruti Paniwala</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34931/3d-dna-3d-de-novo-assembly-3d-dna-pipeline</guid>
	<pubDate>Thu, 28 Dec 2017 10:09:37 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34931/3d-dna-3d-de-novo-assembly-3d-dna-pipeline</link>
	<title><![CDATA[3d-dna: 3D de novo assembly (3D DNA) pipeline]]></title>
	<description><![CDATA[<p>This code is designed to enable anyone to reproduce the Hs2-HiC and the AaegL4 genomes reported in:&nbsp;<a href="http://science.sciencemag.org/content/early/2017/03/22/science.aal3327.full">Dudchenko et al., De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science, 2017.</a></p>
<p>Unless otherwise noted, all terminology below is consistent with this paper, and all references to figures and tables in this readme refer to this paper. Specifically, some of the terminology used below is outlined in&nbsp;<code>Figure S2</code>. The assembly procedure is described in detail in the&nbsp;<a href="http://science.sciencemag.org/content/suppl/2017/03/22/science.aal3327.DC1?_ga=1.9816115.760837492.1490574064">Supporting Online Materials</a>, specifically in the section labelled &ldquo;Pipeline description&rdquo;.</p>
<p>In addition, the pipeline uses tools and methods from&nbsp;<a href="http://www.cell.com/cell-systems/abstract/S2405-4712(16)30219-8">Juicer (Durand &amp; Shamim et al., Cell Systems, 2016)</a>&nbsp;and&nbsp;<a href="http://www.cell.com/cell-systems/abstract/S2405-4712(15)00054-X">Juicebox (Durand &amp; Robinson et al., Cell Systems, 2016)</a>, as well as additional dependencies noted below.</p>
<p>Feel free to post your questions and comments at:&nbsp;<a href="http://www.aidenlab.org/forum.html">http://www.aidenlab.org/forum.html</a></p>
<p>http://aidenlab.org/documentation.html</p><p>Address of the bookmark: <a href="https://github.com/theaidenlab/3d-dna" rel="nofollow">https://github.com/theaidenlab/3d-dna</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31207/laj-viewing-and-manipulating-the-output-from-pairwise-alignment-programs</guid>
	<pubDate>Wed, 01 Mar 2017 08:35:40 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31207/laj-viewing-and-manipulating-the-output-from-pairwise-alignment-programs</link>
	<title><![CDATA[Laj: viewing and manipulating the output from pairwise alignment programs]]></title>
	<description><![CDATA[<p>Laj is a tool for viewing and manipulating the output from pairwise alignment programs such as <a href="http://bio.cse.psu.edu/">blastz</a>. It can display interactive dotplot, pip, and text representations of the alignments, a diagram showing the locations of exons and repeats, and annotation links to other web sites containing additional information about particular regions.</p>
<p>The program is written in Java in order to provide a graphical user interface that is portable across a variety of computer platforms; indeed its name stands for "Local Alignments with Java". Currently it exists in two forms, a stand-alone application and a web-based applet, with slightly different capabilities.</p><p>Address of the bookmark: <a href="http://www.bx.psu.edu/~ratan/" rel="nofollow">http://www.bx.psu.edu/~ratan/</a></p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/36456/alpaca-a-hybrid-strategy-for-assembly-of-genomic-dna-shotgun-sequencing-reads</guid>
	<pubDate>Mon, 30 Apr 2018 04:38:40 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/36456/alpaca-a-hybrid-strategy-for-assembly-of-genomic-dna-shotgun-sequencing-reads</link>
	<title><![CDATA[ALPACA: A hybrid strategy for assembly of genomic DNA shotgun sequencing reads.]]></title>
	<description><![CDATA[<p><span>ALPACA requires Celera Assembler 8.3 or later. It is recommended to build Celera Assembler from source. (Why? The pre-built binaries CA_8.3rc1 and CA8.3rc2 will work for any large data set.&nbsp;</span></p>
<p><span>Detail paper at&nbsp;https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3927-8</span></p><p>Address of the bookmark: <a href="https://github.com/VicugnaPacos/ALPACA" rel="nofollow">https://github.com/VicugnaPacos/ALPACA</a></p>]]></description>
	<dc:creator>Seema Singh</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/31251/bioinformatics-opening-at-icgeb-new-delhi</guid>
  <pubDate>Thu, 02 Mar 2017 04:16:36 -0600</pubDate>
  <link></link>
  <title><![CDATA[Bioinformatics opening at ICGEB NEW DELHI]]></title>
  <description><![CDATA[
<p>ICGEB NEW DELHI</p>

<p>Applications are invited for:</p>

<p>Junior Research Fellow, in a DBT funded project, is available in Translational Health Group, ICGEB, New Delhi</p>

<p>Qualifications:</p>

<p>Education: M.Sc. (preferably in Biotechnology, Life Sciences or Zoology, Chemistry, Bioinformatics). Candidates with hands on experience on GC-MS data acquisition and analysis will be given preference. Bioinformatics expertise required.</p>

<p>Fellowship: As per DBT guidelines.</p>

<p>Tenure: The position is purely on temporary basis with an initial tenure of six months and based on satisfactory performance may continue until the completion of the project.</p>

<p>Closing date for applications: 04/03/2017</p>

<p>Please send a "TWO PAGE" CV by email to:  th.icgeb@gmail.com on or before the last date.</p>

<p>Research Associate, in a DBT funded project, is available in Translational Health Group, ICGEB, New Delhi</p>

<p>Qualifications:</p>

<p>Education: Ph.D. (in Biology, Biotechnology, Chemistry, Bioinformatics). Candidates with hands on experience on GC-MS data acquisition and analysis will be given preference. </p>

<p>Fellowship: As per DBT guidelines.</p>

<p>Tenure: The position is purely on temporary basis with an initial tenure of six months and  based on satisfactory performance may continue until the completion of the project.</p>

<p>Closing date for applications: 04/03/2017</p>

<p>Please send a "TWO PAGE" CV by email to: th.icgeb@gmail.com on or before the last date.</p>
]]></description>
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