BOL: Related items

J-Circos

Shruti Paniwala — Fri, 17 Feb 2017 09:06:54 -0600

Circos plot tool (J-Circos) that is an interactive visualization tool that can plot Circos figures, as well as being able to dynamically add data to the figure, and providing information for specific data points using mouse hover display and zoom in/out functions. J-Circos uses the Java computer language to enable it to be used on most operating systems (Windows, MacOS, Linux). Users can input data into J-Circos using flat data formats, as well as from the GUI. J-Circos will enable biologists to better study more complex chromosomal interactions and fusion transcripts that are otherwise difficult to visualize from next-generation sequencing data.

Address of the bookmark: http://www.australianprostatecentre.org/research/software/jcircos

HaploMerger2: rebuilding both haploid sub-assemblies from high-heterozygosity diploid genome assembly

BioStar — Thu, 27 Sep 2018 07:08:47 -0500

HM2 can process any diploid assemblies, but it is especially suitable for diploid assemblies with high heterozygosity (≥3%), which can be difficult for other tools. This pipeline also implements flexible and sensitive assembly error detection, a hierarchical scaffolding procedure and a reliable gap-closing method for haploid sub-assemblies.

Source code, executables and the testing dataset are freely available at https://github.com/mapleforest/HaploMerger2/releases/.

Address of the bookmark: https://github.com/mapleforest/HaploMerger2/releases/

DIAL

Abhimanyu Singh — Wed, 01 Mar 2017 08:42:28 -0600

A computational pipeline for identifying single-base substitutions between two closely related genomes without the help of a reference genome. DIAL works even when the depth of coverage is insufficient for de novo assembly, and it can be extended to determine small insertions/deletions. Our main motivation is to use this tool to survey the genetic diversity of endangered species as the identified sequence differences can be used to design genotyping arrays to assist in the species' management.

http://www.bx.psu.edu/~ratan/

Address of the bookmark: http://www.bx.psu.edu/miller_lab/

Referee: Genome assembly quality scores

Jit — Sun, 04 Nov 2018 16:44:30 -0600

Modern genome sequencing technologies provide a succint measure of quality at each position in every read, however all of this information is lost in the assembly process. Referee summarizes the quality information from the reads that map to a site in an assembled genome to calculate a quality score for each position in the genome assembly.

We accomplish this by first calculating genotype likelihoods for every site. For a given site in a diploid genome, there are 10 possible genotypes (AA, AC, AG, AT, CC, CG, CT, GG, GT, TT). Referee takes as input the genotype likelihoods calculated for all 10 genotypes given the called reference base at each position.

Referee is a program to calculate a quality score for every position in a genome assembly. This allows for easy filtering of low quality sites for any downstream analysis.

https://github.com/gwct/referee

Address of the bookmark: https://gwct.github.io/referee/#

Evaluation of genome assembly software based on long reads

BioStar — Fri, 01 Feb 2019 11:55:54 -0600

TGS technologies have been used to produce highly accurate de novo assemblies of hundreds of microbial genomes and highly contiguous reconstructions of many dozens of plant and animal genomes, enabling new insights into evolution and sequence diversity. They have also been applied to resequencing analyses, to create detailed maps of structural variations in many species. Also, these new technologies have been used to fill in many of the gaps in the human reference genome.

In this report, we compare and evaluate several genome assembly software based on TSG technology. The experimentation has been performed on 4 reference genomes and the results evaluated with the QUAST software. The 11 software that have been evaluated are: Celera Assembler , Falcon , Miniasm, Newbler , SGA Assembler, Smartdenovo, Abruijn, Ra, DBG2OLC, Spades and Cerulean. The first 8 software use only long reads, while the 3 last software can merge long and short reads

GMASS: a novel measure for genomeassembly structural similarity

Abhimanyu Singh — Sun, 14 Apr 2019 20:35:40 -0500

The GMASS score is a novel measure for representing structural similarity between two assemblies. It will contribute to the understanding of assembly output and developing de novo assemblers.

https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-019-2710-z

Address of the bookmark: http://bioinfo.konkuk.ac.kr/GMASS/htdocs/syncircos.php

RefKA: A fast and efficient long-read genome assembly approach for large and complex genomes

Rahul Nayak — Fri, 01 May 2020 03:00:40 -0500

RefKA, a reference-based approach for long read genome assembly. This approach relies on breaking up a closely related reference genome into bins, aligning k-mers unique to each bin with PacBio reads, and then assembling each bin in parallel followed by a final bin-stitching step.

Address of the bookmark: https://github.com/AppliedBioinformatics/RefKA

The 10th North East Bioinformatics Network (NEBINet) Annual Coordinators' Meet

Jit — Sat, 18 Nov 2017 15:02:44 -0600

The 10th North East Bioinformatics Network (NEBINet) Annual Coordinators' Meet organised by the Bioinformatics Centre, St Edmund's College, Shillong and sponsored by the Department of Biotechnology, Government of India, was held at St Edmund's College Auditorium here on Thursday. Meghalaya Governor Ganga Prasad graced the inaugural programme as chief guest.
In his inaugural address, the Governor said the panorama of scientific scenario has greatly changed over the years, the thrust areas have undergone a metamorphosis but the conceptual underpinning of the basic sciences still continues.
"Of late, the activity of basic research has been intricately intertwined with technology. And we are determined to carry forward this change, for it is through technology that science can actually reach the masses in our country and afar, and the changing times have also inculcated a culture of cross-departmental and interdisciplinary research. Science and technology has always played a pivotal role in taking a nation towards greater heights by ways of innovations and inventions," he added.
Prasad also hoped that discussions, suggestions and sharing of innovative ideas during the two-day 10th NEBINet Annual Coordinators' Meet will open up new avenues to make substantial advancement in Biological Sciences which will provide a platform for proper and effective delivery mechanism for the common man.
During the inaugural function, Advisor of Department of Biotechnology Dr T Madhan Mohan gave an overview of the NEBINet and Bioinformatics programme.
President of Epygen Biotech FZ LLC, Dubai, UAE, Dr Debayan Ghosh, delivered the keynote address.
St Edmund's College governing body secretary Brother Simon Coelho and St Edmund's College Principal Dr Sylvanus Lamare also spoke during the function.

Bioinformatics postdoctoral position at inStem

Tue, 13 Feb 2018 03:18:54 -0600

One postdoctoral position is available in the area of Bioinformatics. This position is available through a highly collaborative project involving multiple labs.

The primary focus here would be to analyse and integrate high throughput data from various aspects of translation regulation including non-coding RNAs, mRNAs and modification of ribosomal RNA. We request the interested candidates to approach either

Dasaradhi Palakodeti (dasaradhip@instem.res.in)
Or
Ravi Muddashetty (ravism@instem.res.in)

Bioinformatics postdoctoral position at CSIR - Institute of Himalayan Bioresource Technology, Palampur (H.P.)

Thu, 05 Apr 2018 06:57:17 -0500

Walk-in-Interview is scheduled to be held on the date as mentioned below for selection of Suitable candidates in the following areas under the DBT sponsored projecT on purely temporary basis for the duration of the project(s) or till completion of projects whichever is earlier:

Project Title:
"Exploration of RBP-RNA interactions to reveal the post-transcriptional regulatory impact, and development of related tools and resource server".

Position: Research Associate (1 position)
Age : 35 years as on 02.05.2018
Salary : Rs.36,000/- P.M. + H.R.A.

as per the funds provisions in the respective projects.

Eligibility Criteria :
Essential Qualifications: Ph.D. in any area of Bioinformatics/ Computational Biology/ Life Sciences.
Desirable: Candidate having experience in Bioinformatics will be preferred.

Essential Qualifications: M.Tech. in Bioinformatics/ Computation Biology/ Computer Science or any related area with 3 years of research experience.

Selection Procedure : Walk In Interview

Date : 02 May, 2018
Time : 9:30 A.M.
Venue : CSIR-IHBT Palampur (H.P.)

For more info refer to following doc:

http://ihbt.res.in/components/com_chronoforms5/chronoforms/uploads/Recruitment/20180404125233_AdvtNo11_2018.pdf