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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/31353?offset=1230</link>
	<atom:link href="https://bioinformaticsonline.com/related/31353?offset=1230" rel="self" type="application/rss+xml" />
	<description><![CDATA[]]></description>
	
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/42275/frequent-parameters-for-bioinformatics-tools</guid>
	<pubDate>Tue, 27 Oct 2020 19:42:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/42275/frequent-parameters-for-bioinformatics-tools</link>
	<title><![CDATA[Frequent parameters for bioinformatics tools !]]></title>
	<description><![CDATA[<div><div>Third party executable parameters and options.</div><div>&nbsp;</div><div>Trimmomatic</div><div>&nbsp;</div><div>&ldquo;ILLUMINACLIP:...:2:30:10&rdquo;</div><div>&ldquo;LEADING:15&rdquo;</div><div>&ldquo;TRAILING:15&rdquo;</div><div>&ldquo;SLIDINGWINDOW:4:20&rdquo;</div><div>&ldquo;MINLEN:20&rdquo;</div><div>&ldquo;TOPHRED33&rdquo;</div><div>&nbsp;</div><div>Filtlong</div><div>--min_length 500</div><div>--min_mean_q 85</div><div>--min_window_q 65</div><div>&nbsp;</div><div>FastQ Screen</div><div>--aligner bowtie2' (bwa for PacBio)</div><div>--subset 1000 (for PacBio)</div><div>&nbsp;</div><div>SPAdes</div><div>--careful</div><div>--disable-gzip-output</div><div>--cov-cutoff auto</div><div>--phred-offset 33</div><div>&nbsp;</div><div>HGAP</div><div>Pbalign.task_options.min_accuracy: 70</div><div>Pbalign.task_options.no_split_subreads: false</div><div>Genomic_consensus.task_options.min_confidence: 40</div><div>falcon_ns.task_options.HGAP_GenomeLength_str:</div><div>6000000</div><div>Pbcoretools.task_options.read_length: 0</div><div>Genomic_consensus.task_options.use_score: 0</div><div>Pbalign.task_options.min_length: 50</div><div>Pbalign.task_options.algorithm_options: --minMatch 12</div><div>--bestn 10 --minPctSimilarity 70.0</div><div>Pbalign.task_options.hit_policy: randombest</div><div>Pbcoretools.task_options.other_filters: rq &gt;= 0.7</div><div>Pbalign.task_options.concordant: false</div><div>Genomic_consensus.task_options.min_coverage: 5</div><div>falcon_ns.task_options.HGAP_SeedCoverage_str: 30</div><div>falcon_ns.task_options.HGAP_AggressiveAsm_bool: false</div><div>Genomic_consensus.task_options.algorithm: best</div><div>falcon_ns.task_options.HGAP_SeedLengthCutoff_str: -1</div><div>Genomic_consensus.task_options.diploid: false</div><div>&nbsp;</div><div>MeDuSa</div><div>-random 100</div><div>&nbsp;</div><div>Prokka</div><div>--usegenus</div><div>--force</div><div>--addgenes</div><div>--rfam</div><div>--rawproduct</div><div>&nbsp;</div><div>cmsearch (taxonomy, 16S)</div><div>--rfam</div><div>--noali</div><div>&nbsp;</div><div>blastn (taxonomy, 16S)</div><div>-evalue 1E-10</div><div>&nbsp;</div><div>blastn (MLST)</div><div>-ungapped</div></div><div><div>-dust no</div><div>-evalue 1E-20</div><div>-word_size 32</div><div>-culling_limit 2</div><div>-perc_identity 95</div><div>&nbsp;</div><div>blastp (VF)</div><div>-culling_limit 2</div><div>&nbsp;</div><div>RGI (ABR)</div><div>--input_type contig</div><div>&nbsp;</div><div>bowtie2 (mapping)</div><div>--sensitive</div><div>&nbsp;</div><div>minimap2 (mapping)</div><div>-a</div><div>-x map-ont</div><div>&nbsp;</div><div>samtools mpileup (SNP&nbsp;detection)</div><div>-uRI</div><div>&nbsp;</div><div>bcftools call (SNP detection)</div><div>--variants-only</div><div>--skip-variants indels</div><div>--output-type v</div><div>--ploidy 1</div><div>-c</div><div>&nbsp;</div><div>SNPsift filter (SNP detection)</div><div>"( QUAL &gt;= 30 ) &amp; (( na FILTER ) | (FILTER = 'PASS')) &amp;</div><div>( DP &gt;= 20 ) &amp; ( MQ &gt;= 20 )"</div><div>&nbsp;</div><div>SNPeff ann (SNP detection)</div><div>-nodownload</div><div>-no-intron</div><div>-no-downstream</div><div>-no SPLICE_SITE_REGION</div><div>-upDownStreamLen 250</div><div>&nbsp;</div><div>bcftools consensus</div><div>(phylogenetic tree)</div><div>--haplotype 1</div><div>&nbsp;</div><div>fasttreemp</div><div>-nt</div><div>-boot 100</div><div>&nbsp;</div><div>roary</div><div>-e</div><div>-n</div><div>-cd 100</div><div>-g 100000</div></div>]]></description>
	<dc:creator>BioStar</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/2423/cancers-origins-revealed</guid>
	<pubDate>Thu, 15 Aug 2013 13:06:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/2423/cancers-origins-revealed</link>
	<title><![CDATA[Cancer's origins revealed]]></title>
	<description><![CDATA[<p>Researchers have provided the first comprehensive compendium of mutational processes that drive tumour development. Together, these mutational processes explain most mutations found in 30 of the most common cancer types. This new understanding of cancer development could help to treat and prevent a wide-range of cancers.<br /><br />More at &gt;&gt; http://www.sanger.ac.uk/about/press/2013/130814.html</p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/42664/common-bioinformatics-interview-questions</guid>
	<pubDate>Sat, 23 Jan 2021 06:07:50 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/42664/common-bioinformatics-interview-questions</link>
	<title><![CDATA[Common Bioinformatics Interview Questions !]]></title>
	<description><![CDATA[<p>The possibility of an interview for a bioinformatics position in the life sciences may be very disquieting, but the same concerns emerge time and again in my experience. So, it is exceedingly worthwhile to plan for future bioinformatics interview questions. Doing this will really give you the advantage in obtaining the position.</p><p>The following 5 questions are those that I have heard many times during the job-search process. There is no reason for not planning responses in such situations.</p><p><strong>1. Tell Us About Yourself</strong><br />This is a very typical opener in interviews. It's a perfect question to ask, and getting something planned will really help you concentrate and ease in the conversation. However, you need to make sure that your response is applicable to the job you're interviewing.<br />It's probably better to keep your answer professional. Try to include these in the answer as well: where did your love of science and bioinformatics come from? How the heck did you end up in this field? Why programming and scripting ?</p><p><strong>2. What is your plan for your bioinformatics career? / How do you look at yourself in five years? / How are your personal objectives to accomplish these goals / What are the plan for your research fundings ?</strong></p><p>Your CV/resume has already impressed the selection panel if you have been invited for an interview. The questions from the bioinformatics interview team provide an incentive for you to market yourself and illustrate the work in question with the most appropriate knowledge.</p><p><strong>3. What do you understand about the job description/What would your suggested research path be if you were a successful candidate?</strong><br />Summarize the specifics of the advertised bioinformatics position in your own words. Follow on with some suggestions of how you want to extend your research and create your own projects within the community.</p><p><strong>4. Will you work as a group or do you want to work on your own?</strong><br />This requirement can vary from jobs to job, so when addressing, be alert. A company/research PI may need a bioinformatician that is able to work on a single project autonomously, or they may need a person who can help direct and organize a team. In your response, refer to the job description.</p><p><strong>5. What particular methods have you used to date with your experiments?</strong><br />You might have experience with all the laboratory techniques described in the job description, but stress the ones you highly experienced with. Highlight your professional abilities and stress that you are extremely capable of mastering new techniques with others ...</p><p>At the end of the day, remember that you're questioning the jury as well as they're interviewing you. You will ought to think of any questions you would like the interview panel to pose. This indicates that you have done your homework and serious about the position.</p><p>All the best for your future job interview.</p>]]></description>
	<dc:creator>Jit</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/videolist/watch/2464/computer-theory-genetics-george-chao-at-tedxumnsalon</guid>
	<pubDate>Thu, 15 Aug 2013 22:08:10 -0500</pubDate>
	<link>https://bioinformaticsonline.com/videolist/watch/2464/computer-theory-genetics-george-chao-at-tedxumnsalon</link>
	<title><![CDATA[Computer Theory & Genetics: George Chao at TEDxUMNSalon]]></title>
	<description><![CDATA[<iframe width="" height="" src="https://www.youtube-nocookie.com/embed/7_GL17oiak8" frameborder="0" allowfullscreen></iframe>George Chao is an undergraduate senior studying Genetics and Computer Science at the University of Minnesota. Having started genetics research as soon as he entered the university, he has worked in labs spanning multiple disciplines as well as in Japan. Some of these researches include developmental genetics in Drosophila, computational techniques for analyzing protein interactions, and helping with the development of algorithms to analyze motion capture data of patients with neck pain. During this time, George steadily developed a fascination with the field of bioinformatics, the study of using computational techniques to learn from genetic data. He would like to go into a career of research into the application of bioinformatics in various fields.

----

The individuals involved with TEDxUMN have a passion for bringing together the great thinkers at the University of Minnesota and giving them the opportunity to share their ideas worth spreading and to discuss our shared future. We provide these great people the opportunity to share these ideas on a global stage and with an incredibly diverse audience. We believe in the power of ideas to change attitudes, lives and ultimately the world.

Check out TEDxUMN at http://www.TEDxUMN.com/

In the spirit of ideas worth spreading, TEDx is a program of local, self-organized events that bring people together to share a TED-like experience. At a TEDx event, TEDTalks video and live speakers combine to spark deep discussion and connection in a small group. These local, self-organized events are branded TEDx, where x = independently organized TED event. The TED Conference provides general guidance for the TEDx program, but individual TEDx events are self-organized.* (*Subject to certain rules and regulations)]]></description>
	
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/43044/kanthida-lab</guid>
  <pubDate>Wed, 28 Apr 2021 02:27:22 -0500</pubDate>
  <link></link>
  <title><![CDATA[Kanthida Lab !]]></title>
  <description><![CDATA[
<p>Research Interest: </p>

<p>Bioinformatics </p>

<p>High-throughput and high-dimensional data analysis</p>

<p>Microbiome data analysis (Main focus)</p>

<p>Next-generation and third-generation sequencing data analysis for genomics</p>

<p>Gene expression data analysis</p>

<p>Machine learning for biological data</p>

<p>Biomarkers identification </p>

<p>Database and web-application for biological data</p>

<p>More at <br />https://sites.google.com/mail.kmutt.ac.th/kanthida-k/home?authuser=0</p>
]]></description>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/2680/4-positions-in-high-throughput-computational-metagenomics-and-systems-biology-of-natural-products</guid>
  <pubDate>Tue, 20 Aug 2013 08:42:29 -0500</pubDate>
  <link></link>
  <title><![CDATA[4 positions in high throughput computational metagenomics and systems biology of natural products]]></title>
  <description><![CDATA[
<p>The Research and Innovation Centre at the Fondazione Edmund Mach (CRI-FEM) is a major international research institution with strong and expanding research interests in Fruit Genomics, Quality Health and Nutrition of Agricultural Products, Agro-ecosystems Sustainability, Biodiversity and Molecular Ecology.</p>

<p>CRI-FEM hosts GMPF, an International PhD Program in Genomics and Molecular Physiology of Fruit Crops and Fox-Lab, an international initiative in forest and wood research.<br />4 positions in high throughput computational metagenomics and systems biology of natural products - deadline September 30th, 2013</p>

<p>To support interdisciplinary research, CRI-FEM has established the Computational Biology Centre (CBC).</p>

<p>The mission of CBC is to develop systems-level integrative approaches connecting genotype to phenotype with a special focus on genome-wide analyses and next generation sequencing technologies. </p>

<p>CRI-FEM is seeking to attract 4 high calibre scientists in the areas of high throughput computational metagenomics and systems biology of natural products.</p>

<p>Here below the list of the 4 positions:</p>

<p>http://www.fmach.it/eng/Servizi-Generali/Lavora-con-noi/Annunci-lavoro-e-borse-di-studio/Details-of-the-5-positions-in-high-throughput-computational-metagenomics-and-systems-biology-of-natural-products-deadline-September-30th-2013/Post-doc-in-Metagenomics-screening-and-characterization-of-bioactive-microbial-compounds-130_CRI_MSC</p>

<p>http://www.fmach.it/eng/Servizi-Generali/Lavora-con-noi/Annunci-lavoro-e-borse-di-studio/Details-of-the-5-positions-in-high-throughput-computational-metagenomics-and-systems-biology-of-natural-products-deadline-September-30th-2013/Post-doc-in-Modeling-transcriptional-control-programs-at-a-genome-wide-scale-131_CRI_TCP</p>

<p>http://www.fmach.it/eng/Servizi-Generali/Lavora-con-noi/Annunci-lavoro-e-borse-di-studio/Details-of-the-5-positions-in-high-throughput-computational-metagenomics-and-systems-biology-of-natural-products-deadline-September-30th-2013/Technologist-in-Purification-of-plant-bioactive-molecules-from-complex-matrixes-132_CRI_PBM</p>

<p>http://www.fmach.it/eng/Servizi-Generali/Lavora-con-noi/Annunci-lavoro-e-borse-di-studio/Details-of-the-5-positions-in-high-throughput-computational-metagenomics-and-systems-biology-of-natural-products-deadline-September-30th-2013/Researcher-in-Methods-for-algorithmic-and-integrative-genomics-for-metagenomics-134_CRI_AIG</p>

<p>For more information on the CBC or informal inquiries on the advertised positions please contact Dr Duccio Cavalieri (e-mail duccio.cavalieri@fmach.it).</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43323/biostarhandbook</guid>
	<pubDate>Fri, 27 Aug 2021 01:31:01 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43323/biostarhandbook</link>
	<title><![CDATA[biostarhandbook]]></title>
	<description><![CDATA[<p>Nice book collection for bioinformatician ... highly recommended.</p><p>Address of the bookmark: <a href="https://www.biostarhandbook.com/" rel="nofollow">https://www.biostarhandbook.com/</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/videolist/watch/2759/dynamic-programming-alignment</guid>
	<pubDate>Thu, 22 Aug 2013 09:38:28 -0500</pubDate>
	<link>https://bioinformaticsonline.com/videolist/watch/2759/dynamic-programming-alignment</link>
	<title><![CDATA[Dynamic Programming Alignment]]></title>
	<description><![CDATA[<iframe width="" height="" src="https://www.youtube-nocookie.com/embed/EWJnDMKBEv0" frameborder="0" allowfullscreen></iframe>lecture 9, Chem. C100, Spring 2013, UCLA]]></description>
	
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	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/44516/16srna-database-download</guid>
	<pubDate>Wed, 24 Apr 2024 04:33:15 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/44516/16srna-database-download</link>
	<title><![CDATA[16sRNA Database Download]]></title>
	<description><![CDATA[<p>Downloading 16S rRNA databases can be crucial for various bioinformatics analyses, especially in microbiome research. However, it's important to note that databases can vary based on your specific needs, such as the taxonomic coverage you require or the type of analysis you're performing. Here's a general guideline on how you can obtain 16S rRNA databases:</p><ol>
<li>
<p><span>NCBI (National Center for Biotechnology Information)</span>:</p>
<ul>
<li>NCBI provides various databases related to genetic information, including 16S rRNA sequences.</li>
<li>You can access the 16S ribosomal RNA sequences from NCBI's Nucleotide database (<a href="https://www.ncbi.nlm.nih.gov/nucleotide/" target="_new">https://www.ncbi.nlm.nih.gov/nucleotide/</a>).</li>
<li>Perform a search using keywords like "16S rRNA" or specific bacterial names to find relevant sequences.</li>
<li>You can download sequences individually or in batches using the provided tools.</li>
</ul>
</li>
<li>
<p><span>GreenGenes</span>:</p>
<ul>
<li>GreenGenes is a widely used 16S rRNA gene sequence database.</li>
<li>You can access it at <a target="_new">http://greengenes.secondgenome.com/</a>.</li>
<li>GreenGenes provides precompiled databases for various purposes, including classification, alignment, and phylogenetic analysis.</li>
</ul>
</li>
<li>
<p><span>SILVA</span>:</p>
<ul>
<li>SILVA (<a href="https://www.arb-silva.de/" target="_new">https://www.arb-silva.de/</a>) is another comprehensive database for ribosomal RNA (rRNA) sequences.</li>
<li>It covers not only 16S rRNA but also other ribosomal RNA sequences.</li>
<li>SILVA provides precompiled databases for various purposes, including taxonomic classification and alignment.</li>
</ul>
</li>
<li>
<p><span>Ribosomal Database Project (RDP)</span>:</p>
<ul>
<li>RDP (<a target="_new">http://rdp.cme.msu.edu/</a>) is a curated database that offers 16S rRNA sequences.</li>
<li>It provides tools for sequence analysis and classification.</li>
<li>You can download sequences and taxonomy information from their website.</li>
</ul>
</li>
<li>
<p><span>QIIME (Quantitative Insights Into Microbial Ecology)</span>:</p>
<ul>
<li>QIIME (<a href="https://qiime2.org/" target="_new">https://qiime2.org/</a>) is a widely used bioinformatics platform for microbiome analysis.</li>
<li>It provides tools for analyzing microbial communities, including processing 16S rRNA sequences.</li>
<li>QIIME often includes its own preprocessed 16S rRNA databases that can be used for analysis within the platform.</li>
</ul>
</li>
</ol><p>Before downloading any database, make sure to read the terms of use and citation requirements, as some databases may have specific usage policies. Additionally, consider the compatibility of the database with your analysis pipeline and software tools.</p><p>&nbsp;</p><p>NCBI 16s RNA database location&nbsp;ftp://ftp.ncbi.nih.gov/blast/db/16SMicrobial.tar.gz</p>]]></description>
	<dc:creator>LEGE</dc:creator>
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  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/4178/phd-position-in-biochemistry-towards-bioinformatics-at-the-department-of-biochemistry-and-biophysics</guid>
  <pubDate>Tue, 03 Sep 2013 06:09:03 -0500</pubDate>
  <link></link>
  <title><![CDATA[PhD position in biochemistry towards bioinformatics at the Department of Biochemistry and Biophysics.]]></title>
  <description><![CDATA[
<p>PhD position in biochemistry towards bioinformatics at the Department of Biochemistry and Biophysics. Reference number: SU FV-2293-13. Deadline for application: September 10, 2013.</p>

<p>Project title: Functional Inference from Domain Architecture and Orthology</p>

<p>Requirements</p>

<p>To be accepted as a PhD student, credits corresponding to four years of full-time studies at the undergraduate level are required, including credits corresponding to at least two years of fulltime studies in chemistry, life sciences or physics, depending on the program. The credits should include courses at the advanced level (second cycle) corresponding to one year and of these one semester should be a degree thesis. In order to facilitate the evaluation of merits and suitability for the PhD studies the curriculum vitae (CV) should contain information about the extent and focus of the academic studies. The quantity (as part of an academic year) and the quality mark of courses in chemistry and physics are of particular interest. The title, number of credits and the length in full-time months of undergraduate thesis and project work, should be specified.</p>

<p>Information</p>

<p>More information about the project can be provided by the project leader. General information about the PhD training program may be requested from the director of graduate studies, Stefan Nordlund, stefan@dbb.su.se or from Lena Mäler, Head of Department (prefekt), lenam@dbb.su.se</p>

<p>Further information on the web:</p>

<p>The Department of Biochemistry and Biophysics: www.dbb.su.se</p>

<p>Stockholm University: www.su.se/english</p>

<p>Faculty of Science: www.science.su.se/english</p>

<p>The handbook for postgraduate students: www.doktorandhandboken.nu/english</p>

<p>Application The application should contain a personal letter (a letter of intent explaining why you are interested in the specific project, why you are interested in studying for a PhD, what you hope to accomplish during your PhD studies, and what skills you can bring to this project), a curriculum vitae, a list of two persons who may act as referees (with telephone numbers and e-mail addresses), copies of degree certificates and transcripts of academic records, and a copy of your undergraduate thesis and articles, if any.</p>

<p>In order to apply for this position, please use the Stockholm University web-based application form (where it is possible to select language):</p>

<p>To the application form for this position.</p>

<p>Welcome with your application no later than September 10, 2013.</p>

<p>Project leader: Erik Sonnhammer, Erik.Sonnhammer@sbc.su.se,<br />www.sonnhammer.sbc.su.se</p>

<p>Advertisement: http://www.su.se/english/about/vacancies/phd-studies/phd-position-in-biochemistry-towards-bioinformatics-1.143446</p>
]]></description>
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