More at sandbox.bio

Address of the bookmark: http://sandbox.bio

No.: SPU/CISST/Advt./2014-15/519

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Applications for the following Contractual Teaching Position are invited for Centre for Interdisciplinary Studies in Science and Technology (CISST), Sardar Patel University:

2. Assistant Professor (ONE) (Contractual)

For the subject of Bioinformatics

Qualifications:

(I) Good academic record as defined by the concerned university with at least 55 % marks (or an equivalent grade in a point scale wherever grading system is followed) at the Master’s level

(II) Ph.D. degree in the concerned subject or in a relevant interdisciplinary subject
from an Indian University or NET/SLET clearance Contractual appointment carries a total Fixed Emoluments of Rs. 30,000/- p.m without any assurance of permanent Positions and related benefits.

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Applicants who are in service should apply through their present employers. Candidates called for interview shall be required to attend at their own cost.

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The last date of receipt of application by the University is 30th April, 2014

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• In a GitHub or GitLab repository
• Free to use
• Written in markdown or similar

NOTE: This list of courses is selected only based on the above criteria.
There are no checks on quality.

https://glittr.org/?per_page=25&sort_by=stargazers&sort_direction=desc

Address of the bookmark: https://glittr.org/?per_page=25&sort_by=stargazers&sort_direction=desc

Basic Galaxy Tutorial

• RNA-Seq tutorial based on Trapnell et al. (2012) Nature Protocols

In this tutorial we cover the concepts of RNA-Seq differential gene expression (DGE) analysis using a very small synthetic dataset from a well studied organism.

Advanced Galaxy Tutorial

• RNA-Seq (Advanced) Tutorial

In this tutorial we compare the performance of three statistically-based differential expression tools:

* CuffDiff

* EdgeR

* DESeq2

Advanced Command Line Tutorial

• Graphical Output with CummeRbund introduces some basic commands using the cummeRbund package of the R programming language

You will need to install R, RStudio and cummeRbund on your PC (explained in the Tutorial). You will learn how to produce graphical output from RNA-Seq analysis previously done using a Cuffdiff analysis.

Variant Detection

Basic Galaxy Tutorial

• Variant Detection tutorial

In this tutorial we cover the concepts of detecting small variants (SNVs and indels) in human genomic DNA using a small set of reads from chromosome 22.

Advanced Galaxy Tutorial

• Variant Detection (Advanced) Tutorial

In this tutorial we compare the performance of three statistically-based variant detection tools:

* SAMtools: Mpileup

* GATK: Unified Genotyper

* FreeBayes

Each of these tools takes as its input a BAM file of aligned reads and generates a list of likely variants in VCF format

Pipelines are for those who are comfortable with using the UNIX command line; and often allow more control over branching and iteration logic.

• WGS/exome GATK-based variant calling pipeline

This is a basic variant-calling and annotation pipeline developed at the Victorian Life Sciences Computation Initiative (VLSCI), University of Melbourne. It is based around BWA, GATK and ENSEMBL and was originally designed for human (or similar) data. The master branch is configured for WGS data; there is an exome branch configured for variant calling in exome data.

To run the pipeline you will need Rubra: https://github.com/bjpop/rubra. Rubra uses the python Ruffus library: http://www.ruffus.org.uk/.

Protocols

• Familial Variant Calling

In this protocol we discuss and outline the process of calling familial related mutations.

• Somatic Variant Calling

In this protocol we discuss and outline the process of identifying somatic variants or mutations.

Assembly

Basic Galaxy Tutorial

• Genome assembly tutorial

In this tutorial we carry out de novo assembly of a microbial genome. We have also written a De novo Genome Assembly for Illumina Data Protocol for a more generic description of the method.

Protocol

• De novo Genome Assembly for Illumina Data

In this protocol we discuss and outline the process of de novo assembly for small to medium sized genomes. Use our Genome assembly tutorial to learn a specific case of using Galaxy to carry out de novo assembly of a microbial genome.

Small RNAs

Basic Galaxy Tutorial

• Quality control for small RNA

This tutorial covers initial steps of the workflow for analysis of short RNA expression such as a quality control of the raw reads, processing of the raw reads for the subsequent analysis and initial quality assessment of the library.

ChIP Seq

Protocol

• ChIP-Seq

In this protocol we discuss ChIP-Seq: a method to analyze the interaction between proteins and DNA.

Amplicons

Protocol

• Amplicon Alignment

In this protocol we discuss and outline the process of aligning custom amplicons using primers for high precision.

Learn Galaxy

Introduction to Galaxy, for those who are very new to Galaxy.

Using Histories and Workflows, for those with some Galaxy knowledge.

The Galaxy project website has many tutorials and screencasts about using Galaxy and the tools, and developing new tools.

Address of the bookmark: https://genome.edu.au/wiki/Learn

1. NCBI (National Center for Biotechnology Information):

   • NCBI provides various databases related to genetic information, including 16S rRNA sequences.
   • You can access the 16S ribosomal RNA sequences from NCBI's Nucleotide database (https://www.ncbi.nlm.nih.gov/nucleotide/).
   • Perform a search using keywords like "16S rRNA" or specific bacterial names to find relevant sequences.
   • You can download sequences individually or in batches using the provided tools.

2. GreenGenes:

   • GreenGenes is a widely used 16S rRNA gene sequence database.
   • You can access it at http://greengenes.secondgenome.com/.
   • GreenGenes provides precompiled databases for various purposes, including classification, alignment, and phylogenetic analysis.

3. SILVA:

   • SILVA (https://www.arb-silva.de/) is another comprehensive database for ribosomal RNA (rRNA) sequences.
   • It covers not only 16S rRNA but also other ribosomal RNA sequences.
   • SILVA provides precompiled databases for various purposes, including taxonomic classification and alignment.

4. Ribosomal Database Project (RDP):

   • RDP (http://rdp.cme.msu.edu/) is a curated database that offers 16S rRNA sequences.
   • It provides tools for sequence analysis and classification.
   • You can download sequences and taxonomy information from their website.

5. QIIME (Quantitative Insights Into Microbial Ecology):

   • QIIME (https://qiime2.org/) is a widely used bioinformatics platform for microbiome analysis.
   • It provides tools for analyzing microbial communities, including processing 16S rRNA sequences.
   • QIIME often includes its own preprocessed 16S rRNA databases that can be used for analysis within the platform.

Before downloading any database, make sure to read the terms of use and citation requirements, as some databases may have specific usage policies. Additionally, consider the compatibility of the database with your analysis pipeline and software tools.

NCBI 16s RNA database location ftp://ftp.ncbi.nih.gov/blast/db/16SMicrobial.tar.gz

No of vacancies: 01

Pay scale:Rs. 15600 – 39100 + 6600/-

Essential Academic Qualifications / Experience : Good academic record as defined by the concerned university with at least 55% marks (or an equivalent grade in a point scale wherever grading system is followed) at the Master's Degree level in a relevant subject from an Indian University, or an equivalent degree from an accredited foreign university.

Besides fulfilling the above qualifications, the candidate must have cleared the National Eligibility Test (NET) conducted by the UGC, CSIR or similar test accredited by the UGC like SLET/ SET.

Notwithstanding anything contained in sub-clauses (a) and (b) to this Clause, candidates, who are, or have been awarded a Ph.D. Degree in accordance with the University Grants Commission (Minimum Standards and Procedure for Award of Ph.D. Degree) Regulations, 2009, shall be exempted from the requirement of the minimum eligibility condition of NET/SLET/SET for recruitment and appointment of Assistant Professor or equivalent positions in Universities/Colleges/Institutions.

NET/SLET/SET shall also not be required for such Masters Programmes in disciplines for which NET/SLET/SET is not conducted.

Apply online: http://www.psc.cg.gov.in/htm/OA_ME2014.html

Last Date for Online Registration: 22/05/2014

For more details: http://www.psc.cg.gov.in/pdf/Advertisement/ADV_ME2014.pdf

As Dennis Ritchie writes in his paper, "The Development of the C Language",
C is quirky, flawed, and an enormous success. While accidents of history surely helped, it evidently satisfied a need for a system implementation language efficient enough to displace assembly language, yet sufficiently abstract and fluent to describe algorithms and interactions in a wide variety of environments.

C++ has its basis in C - extending it by supporting features meant to encourage and support the development of large programs. Perhaps most importantly, it supports object-oriented programming in a familiar setting and framework (that of C). When C++ was created, one of the initial aims was to retain compatibility with C to as large an extent as possible, and retain its spirit and efficiency. It was possible to convert from C to C++ gradually, thus making use of C++ (initally, at least) as a "better C", and moving on to using other features. This allowed many C programmers to learn C++ quickly (though using C++ effectively requires a major mind-shift for many C programmers)
Are you really interested in C/C++ language for the biological programming? If yes there is good news for you. Bio++ 1.9.0 is available with amazing libraries that can help you to solve approximately all problems related with biology.

Some of the new feature has been added in the latest version, these are as follows:

Support for codon models (including non-homogenous models),
Tools for manipulating Hidden Markov Models,
Improved numerical tools (numerical derivatives, parameter transforms...),
A new library, Bio++ RAA (Remote Acnuc Access), allowing you to fetch public databases like GenBank, EMBL or SwissProt,
Algorithms for plotting trees, with support for vector formats like SVG, Fig or LaTeX-PGF.
So get relax and solve the HMM problems with an ease with Bio++. J
Now the time has been change, the biological programmers are ready to use the C++ libraries of biology. These library are designed in order to reduce the C++ long codes in a small and handy for the biological programmers. Basically, Bio++ is a set of C++ libraries for Bioinformatics, including sequence analysis,phylogenetics, molecular evolution and population genetics.
Bio++ is designed in an extensible object-oriented way, in the C++ language.

Some of the unique features of the libraries are as follows:
Sequence analysis

Sequence and Site objects, with various Alphabet support (DNA, RNA, Proteins, Codons, any 'Word' of a given size).
Several containers available for inner storage, with several implementations. Support for alignments.
Various I/O formats supported: Fasta, Mase, CLustal, Phylip, DCSE, GenBank (sequence only).
Sequence manipulation: truncation, concatenation, sub-sequences, etc.
In silico molecular biology: (reverse) transcription, translation, replication.
Several genetic codes availables: Standard and mitochondrial (vertebrates, echinoderms and other invertabrates)
Amino acids properties: volume, polarity and charge + physico-chemical distance (Miyata and Grantham) + import from any AAIndex entry.
Consensus sequences.
Pairwise alignment.
Similarity score computation.
Sequence bootstrap.
Homogeneity test (Bowker's test).
etc.

Phylogenetics and molecular evolution 
Data structure and IO

Phylogenetic trees.
IO from newick files, with support for multiple entries.

Phylogenetic reconstuction methods

Parsimony (NNI)
Distance matrices estimation and I/O to files in Phylip format.
Distance methods: (U/W)PGMA, NJ, BioNJ.
Maximum likelihood (NNI, including a PhyML-like algorithm).
Mixed distance/ML tree reconstruction (iterative approaches).
Tree consensus methods, bipartitions, bootstrap value computations.

Substitution models

JC, K80, T92, F84, HKY85, TN93, GTR and more for nucleotides,
JC, DSO78, JTT92 + any PAML-formated model description for proteins, with possibility to estimate equilibrium frequencies.
Various codon models: Muse & Gaut 1994, Yang & Nielsen 1998, Goldman & Yang 1994 + user-defined.
Support for rate-across sites models, with virtually any probability distribution, allowing for invariant classes.
Covarion models.
Model including gaps.
Global clock tree likelihood models.
Virtually any kind of non-homogeneous model is supported!
Mixed models (beta).

Molecular evolution tools

Parameter estimation under maximum likelihood.
Ancestral states reconstructions: Marginal likelihood.
(Weighted) substitution mapping.
Sequences simulation under any substitution model, homogeneous or not.

Population genetics

A new file format to deal with codominant markers and bio-sequence data for individuals.
Import and export methods with various population genetics software.
Specific containers for polymorphism data.
Diversity and polymorphism statistics for codominant and sequence data.
Estimation of Wright F-statistics and pairwise genetic distance on codominant markers.
Statistics on synonymous and non synonymous sites for coding sequences
Various 'Neutrality' statistics on sequence data (Tajima, Fu and Li, Rand and Kann ...).
Various measures of linkage disequilibrium.
etc.

Numerical calculus

Numerical tools: extended functions (log, factorial, etc.)
Vector tools: element-wise functions, statistics (mean, var, sd, correlation, information theory)
Classes for matrices implementation.
Linear algebra: eigen decomposition, LU decomposition, inversion, etc.
Random number generation: Quick & Dirty (32bits only), Wichmann and Hill, Knuth. Samplers from probability distributions (uniform, normal, gamma, etc.).
Function object implementation, with first and second order derivatives.
Numerical derivatives computation.
Optimization algorithms: Golden section search, Brent's algorithm, Powell's and Downhill simplex method, but also methods using derivatives like conjugate gradient and Newton's method. Object implementation of these methods, using the event-driven Optmizer interface (works with Function objects).
Statistics: DataTable object, with I/O from CSV files, probability distributions.
etc.

Utils

Files: working on file paths, getting file extensions and names, testing existence, open and store in string arrays, etc.
Text: convert text to any other type and vice versa, remove spaces, tokenize, switch between upper/lower case, etc.
Applications: read options from a file or command line
etc.

Some of the libraries are under development that will be updated by Bio++ developers on there websites.
C/C++ Tutorial
http://www.cbcb.umd.edu/~jeallen/bioinfo/
Tutorial on Bio++
http://162.38.181.25/BioPP/articles/tutorial/index.html
Download Links
http://162.38.181.25/BioPP/articles/download/index.html

Reference:
http://162.38.181.25/BioPP/index.html
Dutheil J, Boussau B. Non-homogeneous models of sequence evolution in the Bio++ suite of libraries and programs. BMC Evol Biol. 2008 Sep 22;8(1):255
Dutheil J, Gaillard S, Bazin E, Glémin S, Ranwez V, Galtier N, Belkhir K. Bio++: a set of C++ libraries for sequence analysis, phylogenetics, molecular evolution and population genetics. BMC Bioinformatics. 2006 Apr 4;7:188.
Dutheil JY, Ganapathy G, Hobolth A, Mailund T, Uyenoyama MK, Schierup MH. Ancestral population genomics: the coalescent hidden Markov model approach. Genetics. 2009 Sep;183(1):259-74.
Nabholz B, Mauffrey J-F, Bazin E, Galtier N, Glémin S. Determination of Mitochondrial Genetic Diversity in Mammals. Genetics. 2008 January; 178(1): 351-361.
Galtier N. A model of horizontal gene transfer and the bacterial phylogeny problem. Syst Biol. 2007 Aug;56(4):633-42.
Dutheil J, Galtier N. Detecting groups of coevolving positions in a molecule: a clustering approach. BMC Evol Biol. 2007; 7: 242.
Boussau B, Gouy M. Efficient likelihood computations with nonreversible models of evolution. Syst Biol. 2006 Oct;55(5):756-68.
Dutheil J, Pupko T, Jean-Marie A, Galtier N. A model-based approach for detecting coevolving positions in a molecule. Mol Biol Evol. 2005 Sep;22(9):1919-28.

Webinar on 'An integrated RNA and DNA approach to unravel genetic regulation in cancer'

Abstract

Whole exome DNA sequencing (WES) or whole genome DNA sequencing (WGS) allows detection of mutations and polymorphisms in all exonic and genomic regions, respectively, while messenger RNA sequencing (RNA-Seq) enables quantitative analysis of gene expression. Mutations in the genome result in diverse transcriptional aberrations that can be missed in a stand-alone WES/WGS analysis. An integration of DNA variant analysis and RNA-Seq analysis enables one to investigate the consequences of genomic changes in the RNA transcripts including germline and somatic changes, imprinting, RNA editing and allele specific expression (ASE). In this webinar, we will demonstrate this integrated approach using Strand NGS to identify high confidence mutations, RNA editing events and ASE in cancer.

Webinar Details

Register here: http://www.strand-ngs.com/webinar_registration

About Speaker:

Dr. Veena Hedatale, has a PhD in Plant Genetics from The Radboud University, Netherlands focused on meiosis and recombination. Her prior academic experience at Cornell University was on genetic mapping and gene transformation in Rice. She has worked with Monsanto, and contributed to data mining, database development as well as gene/promoter/pathway discovery for traits related to yield and stress in crop species. At Strand, Veena has worked on Pharmacogenomic analysis of targets and Gene family analysis projects. Currently, she is part of the Strand NGS Application Science team and is involved in the analysis of next generation sequencing data.

Please feel free to contact us 24/5, for availing free online training or if you have any questions.