BOL: Related items

Next generation sequencing(NGS) books

Abhimanyu Singh — Fri, 30 May 2014 04:48:04 -0500

Employing different technologies, the purpose of NGS platform is to decode the identity or modification on the nucleotides. NGS platforms evolve quickly and capture the main stream.

This bookmark is created to provide NGS online books links.

Address of the bookmark: http://en.wikibooks.org/wiki/Next_Generation_Sequencing_%28NGS%29/Print_version

Scientist E-II or F in Bioinformatics & Computational Biology at Rajiv Gandhi Centre for Biotechnology

Fri, 13 Nov 2015 03:10:32 -0600

Advt. No.RGCB Advt./SCI 2015/1

Rajiv Gandhi Centre for Biotechnology (RGCB) invites applications for the following three faculty scientist positions:

Scientist G in any of the following disciplines of disease biology: Cancer Research/Cardiovascular Biology/Diabetes Biology/Pathogen Biology
Scientist E-II or F in Bioinformatics & Computational Biology
Scientist E-I or E-II in Cancer Research with special emphasis on Human Papillomavirus Research

More at http://rgcb.res.in/wp-content/uploads/2015/11/APPLICATION-FORMAT-FOR-SCIENTISTS.docx

Will MinION Nanopore sequencing increase the number of Next Generation Sequencing projects?

Strand — Tue, 04 Aug 2015 05:14:07 -0500

Will MinION Nanopore sequencing increase the number of Next Generation Sequencing projects?

Post-doctoral position / Scientist (m/f/d) in Bioinformatics

Mon, 15 Mar 2021 11:34:23 -0500

The Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. in Dortmund is looking for a Post-doctoral position / Scientist (m/f/d) in Bioinformatics

More at

https://www.isas.de/files/redaktion/jobs/2021/18.60.1_Postdoc_deNBI_06_21_AS.pdf

https://www.isas.de/en/news/062021-post-doctoral-position-scientist-mfd-in-bioinformatics

Finishing !!

Jit — Sat, 20 May 2017 15:50:20 -0500

The process of finishing a genome and moving it from a draft stage (the result of sequencing and initial assembly) to a complete genome is typically a time and resource intensive task. The advent of new sequencing technologies has come with its own set of opportunities and pitfalls in the finishing process. While genomes can now be sequenced to high redundancy in a cost-effective manner, the process of assembling the genomes is more challenging and often draft genomes are fragmented into hundreds of contigs. Correspondingly, the task of producing the complete genome can involve months of lab work and thousands of finishing experiments and is usually done in large genome centers.

The work in our lab has focussed on computational approaches to speed-up the finishing process. Specifically, we have explored the use of optical mapping and mate-pair data to augment assemblies and direct finishing experiments. The tools developed in our lab have been used in several finishing projects, producing complete genomes (and near-complete ones) with surprisingly little computational and experimental effort (Nagarajan et al., in submission). The executables (as well as source code) for these tools are freely available here:

Scaffolding using Optical Restriction Mapping
Optical Maps are global, ordered maps of restriction site locations in a genome. This information can be quite useful in scaffolding contigs from a shotgun assembly to guide the finishing process. A set of programs to exploit optical maps for assembly can be found here: SOMA v2.0 (63 MB tar.gz file). This version of SOMA contains several improvements to programs in v1.0 as well as new scripts for working with multiple maps, contig graphs and scaffolds.
Augmenting assemblies with mate-pair data
Mate-pair information can be valuable in augmenting short-read assemblies and reconstructing the genome as larger scaffolds. AMOS-Hybrid is a pipeline written in the AMOS framework (open-source assembly tools) to merge arbitrary mated reads into an existing assembly and merge contigs and create scaffolds where possible. Source code and executables for AMOS-Hybrid are available here: AMOS-Hybrid v1.0 (142 MB tar.gz file).
Assembly and sequence-composition guided finishing
Contigs from a shotgun assembly are typically linked together in a graph structure that can serve to guide finishing and in some case close gaps in-silico. Also, in many cases, sequence composition of contigs can provide clues to fill gaps in scaffolds. A set of scripts to automate some of these tasks can be found here: Finishing Scripts v1.0 (63 MB tar.gz file).

http://www.cbcb.umd.edu/finishing/

Address of the bookmark: http://www.cbcb.umd.edu/finishing/

PostDoc position in Bioinformatics - IRCCS - Milan - Italy

Wed, 25 Nov 2015 03:38:38 -0600

PostDoc position in Bioinformatics

A full-time post-doctoral position for a bioinformatician with experience in managing and analyzing NGS data is available in the Laboratory of Molecular Cardiology at Policlinico San Donato-IRCCS, Milan, Italy. For lab publications, see: http://scholar.google.it/citations?user=wAPKO9wAAAAJ&hl=it&oi=ao

https://www.researchgate.net/profile/Fabio_Martelli

The goal of the project is investigating the regulation and functional role of non coding RNAs in tissue response to hypoxia and ischemia.

We are looking for a candidate that is going to interact with biologists and bioinformaticians to manage and analyze NGS data (mostly RNA-seq) and microarray data.

The successful candidate must have a minimum of 3 years experience in dealing with NGS data, a good command of English and written communication and at least 2 relevant publications as first author in peer reviewed international journals. The candidate is expected to interact with a small international group and to be able also to work independently. Knowledge of programming is a definitive plus, as well as a good statistical background and/or wet molecular biology skills.

Salary is proportional to experience and to the publication record (up to 38 000euro/year before taxes and retirement contribution i.e. IRPEF and INPS).

Please, send your CV by e-mail to Fabio Martelli:

fabio.martelli@grupposandonato.it

Location: Laboratory of Molecular Cardiology at Policlinico San Donato-IRCCS, Milan, Italy.
Link: https://www.researchgate.net/profile/Fabio_Martelli
Contact person: Fabio Martelli
Contact email: fabio.martelli@grupposandonato.it

JRF Bioinformatics at CUK

Sat, 28 Nov 2015 03:26:21 -0600

JRF job opportunity in Central University of Kerala (CUK) on temporary basis

Project Title : "Targeting TAL effector mediated susceptibility for durable and broad-spectrum resistance to bacterial blight in Rice"

No. of Post : 01

Qualification : MSc in any subject under Life Science or Bioinformatics/ B.Tech in Bioinformatics + 1 yr experience

Stipend : Rs. 14,000/-

How to apply

Interested candidates are requested to send their applications explaining their interest in the position with an updated CV to Dr. Ginny Antony, Assistant Professor, Department of Plant Science, School of Biological Sciences, Central University of Kerala, Padannakkad, Kasaragod, Kerala - 671 314 email: ginnycuk2013@gmail.com on or before 20th December, 2015.

More at http://cukerala.ac.in/index.php?option=com_content&view=article&id=1022:applications-invited-for-the-post-of-jrf-department-of-plant-science&catid=106&Itemid=593&lang=en

Ranbow: a haplotype assembler for polyploid genomes

Jit — Fri, 01 Jun 2018 07:21:54 -0500

Ranbow is a haplotype assembler for polyploid genomes. It has been developed for the haplotype assembly of the hexaploid sweet potato genome, which is highly heterozygous. Ranbow can also be applied to other polyploid genomes. After a first phasing, Ranbow utilizes the assembled haplotypes to improve the accuracy of variant calling results and to infer the evolutionary history of the organism´s genome. Ranbow has three main modes of function: ranbow hap: for haplotyping ranbow eval: for evaluating of the assemble haplotypes by gold standard (long) reads ranbow phylo: for the phylogenetic analysis

Address of the bookmark: https://www.molgen.mpg.de/ranbow

Assistant Professor - Computational Biology and Bioinformatics in Navi Mumbai

Fri, 04 Dec 2015 20:40:59 -0600

No. ACTREC / ADVT-A/2/2015
Pay in Pay band and Grade Pay : PB-3 (Rs 15,600-39,100) Pay in pay band Rs 21,900+ G.P. of Rs 7,600
Total emoluments = 82,000/- p.m. & nbsp
Educational Qualification : Ph.D. or MD/Ph.D.
Experience : Post MD / Ph.D. Research experience of 5 years The last date of application submission is January 15th, 2016.
Interested candidates shall send the applications through email: office.sao(at)actrec.gov.in.
For More Details : www.actrec.gov.in/data%20files/Vacancies/2015/Faculty-Positions-SOE-24-11-15.pdf

Convert EnsEMBL GTF to Annotation table (Geneid, GeneSymbol, GeneWiseChrLocation, GeneClass, Strand) Raw

EagleEye — Fri, 24 Jun 2016 18:08:49 -0500

Bash Script source:

https://gist.github.com/santhilalsubhash/367befcf5216be4b1fd9

Information:

This script converts EnsEMBL GTF (Ex: https://gist.githubusercontent.com/santhilalsubhash/1e7cca357e52a181dc25/raw/cfb803e07900a2baefbb6534f1299fd30cb57a29/sample.GTF) file to annotation table format. It generated two files
1) Transcript wise chromosome location with information about transcripts (Ex: https://gist.githubusercontent.com/santhilalsubhash/c7dec516e0338503a4b6/raw/de0af1a39f0005c4ce7321c5ae57fc8b4a14c7f4/sample.GTF_enst_annotation.txt)
2) Gene wise chromosome location with information about genes (Ex: https://gist.githubusercontent.com/santhilalsubhash/c92006c5080f0333bec2/raw/d16e0b2440d73b09b486d3c9751cdb248a73fa0b/sample.GTF_ensg_annotation.txt)

Note: You can download GTF files from http://www.ensembl.org/info/data/ftp/index.html