BOL: Related items

Project Assistant BioInformatics

Mon, 02 Nov 2015 03:23:09 -0600

Project Assistant BioInformatics

Eligibility : ME/M.Tech(Bio-Informatics/Bio-Chemistry Engg), MSc(Bio-Informatics), BE/B.Tech

Location : Pune

Last Date : 16 Nov 2015

Hiring Process : Face to Face Interview

No. Bio/NCIM/3

Project Assistant II Jobs opportunity in National Chemical Laboratory (NCL) on temporary basis

Project Code No. : GAP312626

Title of the Project : Microbial ecology and distribution of geochemical cycling genes in an hot spring ecosystem

No. of Post : 01

Qualifications : M.Sc./B.Tech/M.Tech in Computational biology/ Bioinformatics from recognized university with minimum 60 % marks (aggregate)

Desirable : Good knowledge of computational skills, Linux (command line and GUI) and Unix; Perl / Python / R /C-programming. Practical knowledge of analysis of Next generation sequence datasets (amplicon sequencing, whole metagenome, and complete genome sequencing) with reference to microbes. Analysis and statistical validation of NGS data generated from different chemistry platforms. Some wet-lab experience in microbial system would be an added advantage as project involves some travel.

Emoluments : Rs. 16,000/-

Age Limit : 28 years

The application with the above information duly signed together with photo-copies of relevant certificates/testimonials should be addressed to : The Head, NCIM Resource Centre (Attn Dr. M.S. DHARNE), National Chemical Laboratory, Pune 411 008, so as to reach on or before 16th November 2015.

More at http://www.ncl-india.org/files/JoinUs/JobVacancies/TemporaryJobs.aspx?menuid=ql6&childmenustripid=divSubQL6

Pre- or postdoctoral research fellowship in Structural Bioinformatics at Padova

Thu, 05 Nov 2015 22:15:12 -0600

A research fellowship for a software developer is available at the BioComputing UP Laboratory, University of Padova (URL: http://protein.bio.unipd.it/).
A highly motivated and creative candidate is sought to work on structural bioinformatics. Specifically, the project entails the development of novel methods, tools and databases for the analysis of protein structures.

The BioComputing UP Laboratory, headed by Prof. Silvio Tosatto, is a dynamic group of a dozen people working on several aspects of prediction of protein structure & function employing techniques at the intersection between biology, medicine, chemistry, physics & computer science.
Our aim is to integrate the development of novel methods and their application to biologically relevant problems.

We are looking for candidates with a solid Bioinformatics background, programming experience (Python, C++ and/or Java) and good knowledge of molecular biology (protein structure/function). Good knowledge of statistics as well as experience in using database systems (MongoDB, MySQL and/or Postgres) is desirable. Candidates should have a degree with top marks, optionally hold a PhD, and be highly motivated to work on interdisciplinary research. Good knowledge of English, an open-minded spirit, being collaborative and creative are crucial.

The fellowship, which should start as soon as possible, is renewable and initially for one year. It will be commensurate to experience, can be extended depending on performance and may lead to a PhD degree. The successful candidate will be working full-time at the BioComputing UP Laboratory, University of Padova. Travel support for conferences and/or research visits abroad is provided.
To apply, please send your CV, with a motivation letter and brief description of your research background as well as the names of two (or more) references to: biocomp@bio.unipd.it.

Start date: As soon as possible

Duration: 1 year, renewable

Salary on grant: Commesurate to experience

Contact Person (Referent): Silvio Tosatto

Ref. E-Mail: biocomp@bio.unipd.it

Tel: +39 049 827 6269
Fax: +39 049 827 6260

Group Web Page: http://protein.bio.unipd.it/

shovill: Assemble bacterial isolate genomes from Illumina paired-end reads

BioStar — Sat, 02 Jan 2021 07:05:36 -0600

Shovill is a pipeline which uses SPAdes at its core, but alters the steps before and after the primary assembly step to get similar results in less time. Shovill also supports other assemblers like SKESA, Velvet and Megahit, so you can take advantage of the pre- and post-processing the Shovill provides with those too.

Address of the bookmark: https://github.com/tseemann/shovill

Run miniasm assembler on nanopore reads !

Jit — Mon, 18 Dec 2017 04:07:50 -0600

Miniasm is a very fast OLC-based de novo assembler for noisy long reads. It takes all-vs-all read self-mappings (typically by minimap) as input and outputs an assembly graph in the GFA format. Different from mainstream assemblers, miniasm does not have a consensus step. It simply concatenates pieces of read sequences to generate the final unitig sequences. Thus the per-base error rate is similar to the raw input reads.

Find the detail of the reads repeats:

fq2fa ONT_A.fastq ONT_A.fasta

minimap2 -xava-ont ONT_A.fasta ONT_A.fasta -t10 -X > AONT.paf

awk '{if($1==$6){print}}' AONT.paf > AONTself.paf

awk '$5=="-"' AONTself.paf | awk '{print $1}'| sort|uniq > invertedrepeat.list

Generated a few palindrome and repeats plots (highlighting only repeats largest than 10, 20 and 30 kb)

minidot -f 5 -m 30000 AONTself.paf > AONTself30000.eps
sed 's/_template_pass_FAH31515//' AONTself30000.eps > AONTself30000final.eps

minidot -f 5 -m 20000 AONTself.paf > AONTself20000.eps
sed 's/_template_pass_FAH31515//' AONTself20000.eps > AONTself20000final.eps

minidot -f 5 -m 10000 AONTself.paf > AONTself10000.eps
sed 's/_template_pass_FAH31515//' AONTself10000.eps > AONTself10000final.eps

Assemble with miniasm:

miniasm -f ONT_A.fasta AONT.paf > AONT.gfa
grep '^S' AONT.gfa |awk '{print ">"$2"\n"$3}' > AONT_miniasm.fasta

minimap2 -xasm10 AONT_miniasm.fasta AONT_miniasm.fasta -t1 -X > AONT_miniasm.paf

awk '{if($1==$6){print}}' AONT_miniasm.paf > AONT_miniasm_self.paf

minidot -f 5 -m 10000 AONT_miniasm_self.paf > AONT_miniasm_self10000.eps

Njoy the assembly !

SRF/JRF Bioinformatics at National Bureau of Animal Genetic Resources

Thu, 26 Nov 2015 01:54:07 -0600

National Bureau of Animal Genetic Resources

SRF Job vacancies in National Bureau of Animal Genetic Resources (NBAGR) on contractual basis

No. of Posts : 03

Qualifications : Master degree in any one of discipline/ Subjects Biotechnology/Bioinformatics/Life Sciences/ Computer Applications with 4 years/5 years of Bachelor’s degree. Candidates having post graduate degree in above mentioned subjects with 3 years Bachelor’s degree should be NET qualified.

Note : i. The Ph.D. Degree holders are eligible for the posts. ii. The Master’s degree holders with the integrated degrees like B.Tech. & M. Tech. (5 years) are eligible. iii. Graduates with three years Bachelor’s degree, who have acquired professional Master’s degree (3 years) like M.Sc. (Ag.), Masters in Computer Applications, M. Tech., etc., are eligible.

Desirable : The candidates should have zeal to work in R environment and NGS data analysis

Emolument : Rs. 28,000/-

Age Limit : 35 year for Men and 40 years for Women in case of SRF on the date of Interview.
How to apply

Walk-in-Interview is proposed to be held at National Bureau of Animal Genetic Resources, Karnal (Haryana)-132 001 at 10:30 AM on 02.12.2015. Interested candidates may attend Walk-in-Interview at 10:30 AM on 02.12.2015 alongwith original certificates and typed bio-data with one set of attested copies of each their certificates with passport size photograph.

TAREAN: A computational tool for identification and characterization of satellite DNA from unassembled short reads

Surabhi Chaudhary — Tue, 15 May 2018 02:53:11 -0500

TAndem REpeat ANalyzer -TAREAN – is a computational pipeline for unsupervised identification of satellite repeats from unassembled sequence reads. The pipeline uses low-pass whole genome sequence reads and performs their graph-based clustering. Resulting clusters, representing all types of repeats, are then examined for the presence of circular structures and putative satellite repeats are reported.

How to use TAREAN:

Install a local instance of the pipeline using its source code available from bitbucket repository.
Use public Galaxy-based server at https://repeatexplorer-elixir.cerit-sc.cz/. The server is provided in frame of the Elixir CZ project and is maintained by CESNET and CERIT-SC. Simple registration is required to use this service.

Development of TAREAN was supported by ELIXIR CZ research infrastructure project (MEYS Grant No: LM2015047).

References

Novak, P., Avila Robledillo, L., Koblizkova, A., Vrbova, I., Neumann, P., Macas, J. (2017) – TAREAN: a computational tool for identification and characterization of satellite DNA from unassembled short reads. Nucleic Acids Res., doi:10.1093/nar/gkx257

Address of the bookmark: https://bitbucket.org/petrnovak/repex_tarean

CIAB Recruits Scientist, Engineer, Informatics Officer

Sat, 28 Nov 2015 03:40:28 -0600

Center of Innovative and Applied Bioprocessing (CIAB) - Punjab

Center of Innovative and Applied Bioprocessing (CIAB) invites applications to recruit on vacant posts of Scientist, Associate Plant Manager/ Plant Engineer, Informatics Officer. Applications against these Government Jobs can be submitted on or before 7th January 2016.
CIAB Vacancy 2015 Details

9. Research & Development – Informatics Officer
Qualification: M. Tech or M.E. in computer science or information technology or bioinformatics with 4 years of R&D experience (after Master’s Degree) in an R&D organization with work profile of large data assembly, analysis.
Age Limit: 45 years
How to Apply : Duly filled-in applications in prescribed application format along with copies of required documents should be reach to: Chief Exec

npScarf: real-time scaffolder using SPAdes contigs and Nanopore sequencing reads

Shruti Paniwala — Mon, 11 Jun 2018 05:14:57 -0500

npScarf (jsa.np.npscarf) is a program that connect contigs from a draft genomes to generate sequences that are closer to finish. These pipelines can run on a single laptop for microbial datasets. In real-time mode, it can be integrated with simple structural analyses such as gene ordering, plasmid forming.

Address of the bookmark: http://japsa.readthedocs.io/en/latest/tools/jsa.np.npscarf.html

SimLoRD: A read simulator for third generation sequencing reads

Aaryan Lokwani — Wed, 22 Aug 2018 10:40:27 -0500

SimLoRD is a read simulator for third generation sequencing reads and is currently focused on the Pacific Biosciences SMRT error model.

Reads are simulated from both strands of a provided or randomly generated reference sequence.

The reference can be read from a FASTA file or randomly generated with a given GC content. It can consist of several chromosomes, whose structure is respected when drawing reads. (Simulation of genome rearrangements may be incorporated at a later stage.)
The read lengths can be determined in four ways: drawing from a log-normal distribution (typical for genomic DNA), sampling from an existing FASTQ file (typical for RNA), sampling from a a text file with integers (RNA), or using a fixed length
Quality values and number of passes depend on fragment length.
Provided subread error probabilities are modified according to number of passes
Outputs reads in FASTQ format and alignments in SAM format

Address of the bookmark: https://bitbucket.org/genomeinformatics/simlord/

ASSISTANT PROFESSOR Bioinformatics at ALAGAPPA UNIVERSITY

Thu, 03 Dec 2015 23:30:43 -0600

ALAGAPPA UNIVERSITY
Recruitment and Sarkari Naukri for the Post of ASSISTANT PROFESSOR Bioinformatics
Job Description UGC scale of pay is applicable. For eligibility qualifications and other norms, please refer to the ?Instructions to the Candidates? available with the application forms which can be had on payment of Rs.520/- inclusive of Rs.20/- for postage. For SC/ST, Rs.320/- inclusive of Rs.20/- for postage on enclosure of a copy of the community certificate. Payment is to be made by means of Demand Draft drawn on any nationalized bank in favour of ?The Registrar, Alagappa University? payable at Karaikudi. Candidates can also download the application form and instructions to the candidates from httpwww.alagappauniversity.ac Filled-in application should reach on or before 19.10.2015
Salary for Job : Rs.15600-39100+AGP Rs.6000
Education : Good academic record with at least 55 marks (or an equivalent grade in a point scale wherever grading system is followed) at the Masters Degree level in a relevant subject from an Indian University, or an equivalent degree from an accredited foreign university. Besides fulfilling the above qualifications, the candidate must have cleared the National Eligibility Test (NET) conducted by the UGC, CSIR or similar test accredited by the UGC like SLET/SET.Notwithstanding anything contained in sub-clauses (i) and (ii) above, the candidates, who are, or have been awarded a Ph.D. degree in accordance with the University Grants Commission (Minimum Standards and Procedure for Award of Ph.D. Degree) Regulations 2009, shall be exempted from the requirement of the minimum eligibility condition of NET/SLET/SET for recruitment and appointment of Assistant Professor or equivalent positions in Universities/Colleges/Institutions.
Number of Vacancies : 02
Naukri Location : Other City(s) in Tamil Nadu
Address : KARAIKUDI ? 630 003
Last Date to Apply : 2015-12-04
Apply Process : written test/interview