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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/34328?offset=300</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/file/view/36952/getoptspl-file</guid>
	<pubDate>Fri, 15 Jun 2018 04:43:03 -0500</pubDate>
	<link>https://bioinformaticsonline.com/file/view/36952/getoptspl-file</link>
	<title><![CDATA[getopts.pl file]]></title>
	<description><![CDATA[
<p>SSPACE_longread complain for getopts.pl file. </p>

<p>To resolve this, download and have in SSPACED-Longreads folder. </p>

<p>Cheers :)</p>
]]></description>
	<dc:creator>Jit</dc:creator>
	<enclosure url="https://bioinformaticsonline.com/file/download/36952" length="942" type="text/plain" />
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37239/kat-a-k-mer-analysis-toolkit-to-quality-control-ngs-datasets-and-genome-assemblies</guid>
	<pubDate>Fri, 06 Jul 2018 03:36:45 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37239/kat-a-k-mer-analysis-toolkit-to-quality-control-ngs-datasets-and-genome-assemblies</link>
	<title><![CDATA[KAT: a K-mer analysis toolkit to quality control NGS datasets and genome assemblies]]></title>
	<description><![CDATA[<p>KAT is a suite of tools that analyse jellyfish hashes or sequence files (fasta or fastq) using kmer counts. The following tools are currently available in KAT:</p>
<ul>
<li><span>hist</span>: Create an histogram of k-mer occurrences from a sequence file. Adds metadata in output for easy plotting.</li>
<li><span>gcp:</span>&nbsp;K-mer GC Processor. Creates a matrix of the number of K-mers found given a GC count and a K-mer count.</li>
<li><span>comp</span>: K-mer comparison tool. Creates a matrix of shared K-mers between two (or three) sequence files or hashes.</li>
<li><span>sect</span>: SEquence Coverage estimator Tool. Estimates the coverage of each sequence in a file using K-mers from another sequence file.</li>
<li><span>blob</span>: Given, reads and an assembly, calculates both the read and assembly K-mer coverage along with GC% for each sequence in the assembly.SEquence Coverage estimator Tool.</li>
<li><span>filter</span>: Filtering tools. Contains tools for filtering k-mer hashes and FastQ/A files:
<ul>
<li><span>kmer</span>: Produces a k-mer hash containing only k-mers within specified coverage and GC tolerances.</li>
<li><span>seq</span>: Filters a sequence file based on whether or not the sequences contain k-mers within a provided hash.</li>
</ul>
</li>
<li><span>plot</span>: Plotting tools. Contains several plotting tools to visualise K-mer and compare distributions. The following plot tools are available:
<ul>
<li><span>density</span>: Creates a density plot from a matrix created with the "comp" tool. Typically this is used to compare two K-mer hashes produced by different NGS reads.</li>
<li><span>profile</span>: Creates a K-mer coverage plot for a single sequence. Takes in fasta coverage output coverage from the "sect" tool</li>
<li><span>spectra-cn</span>: Creates a stacked histogram using a matrix created with the "comp" tool. Typically this is used to compare a jellyfish hash produced from a read set to a jellyfish hash produced from an assembly. The plot shows the amount of distinct K-mers absent, as well as the copy number variation present within the assembly.</li>
<li><span>spectra-hist</span>: Creates a K-mer spectra plot for a set of K-mer histograms produced either by jellyfish-histo or kat-histo.</li>
<li><span>spectra-mx</span>: Creates a K-mer spectra plot for a set of K-mer histograms that are derived from selected rows or columns in a matrix produced by the "comp".</li>
</ul>
</li>
</ul>
<p>In addition, KAT contains a python script for analysing the mathematical distributions present in the K-mer spectra in order to determine how much content is present in each peak.</p>
<p>This README only contains some brief details of how to install and use KAT. For more extensive documentation please visit:&nbsp;<a href="https://kat.readthedocs.org/en/latest/">https://kat.readthedocs.org/en/latest/</a></p>
<p><a href="https://academic.oup.com/bioinformatics/article/33/4/574/2664339">https://academic.oup.com/bioinformatics/article/33/4/574/2664339&nbsp;</a></p><p>Address of the bookmark: <a href="https://github.com/TGAC/KAT" rel="nofollow">https://github.com/TGAC/KAT</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/37411/my-commonly-used-commands-in-bioinformatics</guid>
	<pubDate>Thu, 26 Jul 2018 04:58:45 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/37411/my-commonly-used-commands-in-bioinformatics</link>
	<title><![CDATA[My commonly used commands in Bioinformatics]]></title>
	<description><![CDATA[<p>FYI, I've found it useful to use MUMmer to extract the specific changes that Racon makes, so I can evaluate them individually:</p><pre><code>minimap -t 24 assembly.fasta long_reads.fastq.gz | racon -t 24 long_reads.fastq.gz - assembly.fasta racon_assembly.fasta
nucmer -p nucmer assembly.fasta racon_assembly.fasta
show-snps -C -T -r nucmer.delta
</code></pre><p>This reports Racon's changes in a table. You can exclude indels with the&nbsp;<code>-I</code>&nbsp;option in&nbsp;<code>show-snps</code>.&nbsp;</p><p>This process (Racon -&gt; MUMmer -&gt; SNP table) solves the problem I originally raised in this issue. So as far as I'm concerned, you can close this issue (or keep it open if you still want to implement some kind of variant table).</p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37785/haplomerger2-rebuilding-both-haploid-sub-assemblies-from-high-heterozygosity-diploid-genome-assembly</guid>
	<pubDate>Thu, 27 Sep 2018 07:08:47 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37785/haplomerger2-rebuilding-both-haploid-sub-assemblies-from-high-heterozygosity-diploid-genome-assembly</link>
	<title><![CDATA[HaploMerger2: rebuilding both haploid sub-assemblies from high-heterozygosity diploid genome assembly]]></title>
	<description><![CDATA[<p><span><span>HM2 can process any diploid assemblies, but it is especially suitable for diploid assemblies with high heterozygosity (&ge;3%), which can be difficult for other tools. This pipeline also implements flexible and sensitive assembly error detection, a hierarchical scaffolding procedure and a reliable gap-closing method for haploid sub-assemblies.</span></span></p>
<p><span>Source code, executables and the testing dataset are freely available at&nbsp;</span><a href="https://github.com/mapleforest/HaploMerger2/releases/" target="">https://github.com/mapleforest/HaploMerger2/releases/</a><span>.</span></p><p>Address of the bookmark: <a href="https://github.com/mapleforest/HaploMerger2/releases/" rel="nofollow">https://github.com/mapleforest/HaploMerger2/releases/</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38008/quast-lg-versatile-genome-assembly-evaluation</guid>
	<pubDate>Thu, 25 Oct 2018 10:46:55 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38008/quast-lg-versatile-genome-assembly-evaluation</link>
	<title><![CDATA[QUAST-LG: Versatile genome assembly evaluation]]></title>
	<description><![CDATA[<p>QUAST-LG-a tool that compares large genomic de novo assemblies against reference sequences and computes relevant quality metrics. Since genomes generally cannot be reconstructed completely due to complex repeat patterns and low coverage regions, we introduce a concept of upper bound assembly for a given genome and set of reads, and compute theoretical limits on assembly correctness and completeness. Using QUAST-LG, we show how close the assemblies are to the theoretical optimum, and how far this optimum is from the finished reference.</p>
<h4>AVAILABILITY AND IMPLEMENTATION:</h4>
<p>http://cab.spbu.ru/software/quast-lg</p><p>Address of the bookmark: <a href="http://cab.spbu.ru/software/quast-lg/" rel="nofollow">http://cab.spbu.ru/software/quast-lg/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38176/asciigenome-genome-browser-based-on-command-line-interface-and-designed-for-running-from-console-terminals</guid>
	<pubDate>Fri, 09 Nov 2018 13:50:04 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38176/asciigenome-genome-browser-based-on-command-line-interface-and-designed-for-running-from-console-terminals</link>
	<title><![CDATA[ASCIIGenome: genome browser based on command line interface and designed for running from console terminals.]]></title>
	<description><![CDATA[<p><code>ASCIIGenome</code>&nbsp;is a genome browser based on command line interface and designed for running from console terminals.</p>
<p>Since&nbsp;<code>ASCIIGenome</code>&nbsp;does not require a graphical interface it is particularly useful for quickly visualizing genomic data on remote servers while offering flexibility similar to popular GUI viewers like&nbsp;<a href="https://www.broadinstitute.org/igv/">IGV</a>.</p>
<p><span>Documentation</span>&nbsp;is at&nbsp;<a href="http://asciigenome.readthedocs.io/en/latest/">readthedocs/asciigenome</a>.</p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="https://github.com/dariober/ASCIIGenome" rel="nofollow">https://github.com/dariober/ASCIIGenome</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41831/merqury-reference-free-quality-and-phasing-assessment-for-genome-assemblies</guid>
	<pubDate>Sat, 06 Jun 2020 05:38:34 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41831/merqury-reference-free-quality-and-phasing-assessment-for-genome-assemblies</link>
	<title><![CDATA[Merqury: reference-free quality and phasing assessment for genome assemblies]]></title>
	<description><![CDATA[<p><span>Often, genome assembly projects have illumina whole genome sequencing reads available for the assembled individual. The k-mer spectrum of this read set can be used for independently evaluating assembly quality without the need of a high quality reference. Merqury provides a set of tools for this purpose.</span></p>
<p><span><a href="https://github.com/marbl/meryl">https://github.com/marbl/meryl</a></span></p><p>Address of the bookmark: <a href="https://github.com/marbl/merqury" rel="nofollow">https://github.com/marbl/merqury</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38481/arcs-scaffolding-genome-drafts-with-linked-reads</guid>
	<pubDate>Mon, 17 Dec 2018 17:40:28 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38481/arcs-scaffolding-genome-drafts-with-linked-reads</link>
	<title><![CDATA[ARCS: scaffolding genome drafts with linked reads]]></title>
	<description><![CDATA[<p>ARCS requires two input files:</p>
<ul>
<li>Draft assembly fasta file</li>
<li>Interleaved linked reads file (Barcode sequence expected in the BX tag of the read header or in the form "@readname_barcode" ; Run&nbsp;<a href="https://support.10xgenomics.com/genome-exome/software/pipelines/latest/what-is-long-ranger">Long Ranger basic</a>&nbsp;on raw chromium reads to produce this interleaved file)</li>
<li></li>
</ul><p>Address of the bookmark: <a href="https://github.com/bcgsc/ARCS/" rel="nofollow">https://github.com/bcgsc/ARCS/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38670/ltr-finder-an-efficient-program-for-finding-full-length-ltr-retrotranspsons-in-genome-sequences</guid>
	<pubDate>Sun, 13 Jan 2019 07:05:53 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38670/ltr-finder-an-efficient-program-for-finding-full-length-ltr-retrotranspsons-in-genome-sequences</link>
	<title><![CDATA[LTR_Finder: an efficient program for finding full-length LTR retrotranspsons in genome sequences.]]></title>
	<description><![CDATA[<p>LTR_Finder is an efficient program for finding full-length LTR retrotranspsons in genome sequences.</p>
<p>The Program first constructs all exact match pairs by a suffix-array based algorithm and extends them to long highly similar pairs. Then Smith-Waterman algorithm is used to adjust the ends of LTR pair candidates to get alignment boundaries. These boundaries are subject to re-adjustment using supporting information of TG..CA box and TSRs and reliable LTRs are selected. Next, LTR_FINDER tries to identify PBS, PPT and RT inside LTR pairs by build-in aligning and counting modules. RT identification includes a dynamic programming to process frame shift. For other protein domains, LTR_FINDER calls ps_scan (from PROSITE,&nbsp;<a href="http://www.expasy.org/prosite/">http://www.expasy.org/prosite/</a>) to locate cores of important enzymes if they occur.</p><p>Address of the bookmark: <a href="https://github.com/xzhub/LTR_Finder" rel="nofollow">https://github.com/xzhub/LTR_Finder</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40792/haslr-a-tool-for-rapid-genome-assembly-of-long-sequencing-reads</guid>
	<pubDate>Fri, 31 Jan 2020 05:50:15 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40792/haslr-a-tool-for-rapid-genome-assembly-of-long-sequencing-reads</link>
	<title><![CDATA[HASLR: a tool for rapid genome assembly of long sequencing reads]]></title>
	<description><![CDATA[<p><span>HASLR is a tool for rapid genome assembly of long sequencing reads. HASLR is a hybrid tool which means it requires long reads generated by Third Generation Sequencing technologies (such as PacBio or Oxford Nanopore) together with Next Generation Sequencing reads (such as Illumina) from the same sample.&nbsp;</span></p><p>Address of the bookmark: <a href="https://github.com/vpc-ccg/haslr" rel="nofollow">https://github.com/vpc-ccg/haslr</a></p>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>

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