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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/34488?offset=600</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/13842/swabs-to-genomes-a-comprehensive-workflow</guid>
	<pubDate>Sun, 10 Aug 2014 03:01:21 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/13842/swabs-to-genomes-a-comprehensive-workflow</link>
	<title><![CDATA[Swabs to Genomes: A Comprehensive Workflow]]></title>
	<description><![CDATA[<p>The sequencing, assembly, and basic analysis of microbial genomes, once a painstaking and expensive undertaking, has become almost trivial for research labs with access to standard molecular biology and computational tools. However, there are a wide variety of options available for DNA library preparation and sequencing, and inexperience with bioinformatics can pose a significant barrier to entry for many who may be interested in microbial genomics. The objective of the present study was to design, test, troubleshoot, and publish a simple, comprehensive workflow from the collection of an environmental sample (a swab) to a published microbial genome; empowering even a lab or classroom with limited resources and bioinformatics experience to perform it.</p><p>Address of the bookmark: <a href="https://peerj.com/preprints/453.pdf" rel="nofollow">https://peerj.com/preprints/453.pdf</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/19090/deeptools</guid>
	<pubDate>Sat, 08 Nov 2014 15:02:08 -0600</pubDate>
	<link>https://bioinformaticsonline.com/news/view/19090/deeptools</link>
	<title><![CDATA[deepTools]]></title>
	<description><![CDATA[<p>deepTools addresses the challenge of handling the large amounts of data that are now routinely generated from DNA sequencing centers. To do so, deepTools contains useful modules to process the mapped reads data to create coverage files in standard bedGraph and bigWig file formats. By doing so, deepTools allows the creation of normalized coverage files or the comparison between two files (for example, treatment and control). Finally, using such normalized and standardized files, multiple visualizations can be created to identify enrichments with functional annotations of the genome.<br /><br />Publicaton: http://nar.oxfordjournals.org/content/early/2014/05/05/nar.gku365.full<br /><br />Source Code and Wiki: https://github.com/fidelram/deepTools/wiki<br /><br />Galaxy Tool Shed repository: http://toolshed.g2.bx.psu.edu/view/bgruening/deeptools<br /><br />and example Galaxy workflows: http://toolshed.g2.bx.psu.edu/view/bgruening/deeptools_workflows</p>]]></description>
	<dc:creator>Martin Jones</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/19633/vital-it</guid>
	<pubDate>Thu, 18 Dec 2014 10:46:59 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/19633/vital-it</link>
	<title><![CDATA[Vital-IT]]></title>
	<description><![CDATA[<p>Vital-IT is a <strong>bioinformatics competence center</strong> that supports and collaborates with life scientists in Switzerland and beyond. The <a href="http://www.vital-it.ch/about/team.php">multi-disciplinary team</a> provides expertise, training and maintains a high-performance computing (HPC) and storage infrastructure, so as to help develop, maintain and extend life science and medical research (<a href="http://www.vital-it.ch/about/activities.php">activities</a>).</p><p>Address of the bookmark: <a href="http://www.vital-it.ch/" rel="nofollow">http://www.vital-it.ch/</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/26993/lastz</guid>
	<pubDate>Mon, 18 Apr 2016 04:41:55 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/26993/lastz</link>
	<title><![CDATA[LASTZ]]></title>
	<description><![CDATA[<p>LASTZ is a program for aligning DNA sequences, a pairwise aligner. Originally designed to handle sequences the size of human chromosomes and from different species, it is also useful for sequences produced by NGS sequencing technologies such as Roche 454.</p>
<p>More at http://www.bx.psu.edu/~rsharris/lastz/</p>
<p>Thesis: http://www.bx.psu.edu/~rsharris/rsharris_phd_thesis_2007.pdf</p><p>Address of the bookmark: <a href="http://www.bx.psu.edu/~rsharris/lastz/" rel="nofollow">http://www.bx.psu.edu/~rsharris/lastz/</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/24762/postdoctoral-fellowship-in-bioinformatics-at-pesolelab</guid>
  <pubDate>Thu, 01 Oct 2015 07:20:48 -0500</pubDate>
  <link></link>
  <title><![CDATA[Postdoctoral Fellowship in Bioinformatics at pesolelab]]></title>
  <description><![CDATA[
<p>Job Description: Bioinformatics postdoc positions are available in the area of genomics with main focus on exome and RNAseq technologies by ultra high-throughput sequencing platforms. Successful applicants should have the following qualities:</p>

<p>1) demonstrated experience in Bioinformatics research,<br />2) programing experience (python and/or R, C and C++ are very welcome),<br />3) knowledge of Linux/Unix environment,<br />4) experience in handling deep-seq data,<br />5) highly motivated and hard working, and<br />6) interested to work with a multi-disciplinary team combining bioinformatics, genomics, computational biology approaches with experimental biology.</p>

<p>Our research interest covers different areas of bioinformatics and genomics in order to achieve a deeper understanding of gene and genome structure and function (please look at our PubMed publications for more details about our research http://www.ncbi.nlm.nih.gov/pubmed/?term=pesole+g).</p>

<p>Interested applicants should email the curriculum vitae to Prof. Graziano Pesole at graziano.pesole@uniba.it or Dr. Ernesto Picardi at Ernesto.picardi@uniba.it.</p>

<p>Start date: immediate</p>

<p>Duration: up to 24 months<br />Contact Person (Referent): Ernesto Picardi<br />Ref. E-Mail: ernesto.picardi@uniba.it<br />Tel: +390805443308<br />Fax: +390805443317</p>

<p>Group Web Page: http://www.pesolelab.it/</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/26179/alignment-of-closely-related-whole-genomesscaffolds</guid>
	<pubDate>Fri, 29 Jan 2016 10:37:27 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/26179/alignment-of-closely-related-whole-genomesscaffolds</link>
	<title><![CDATA[Alignment of closely related whole genomes/scaffolds]]></title>
	<description><![CDATA[<p>With the relative ease and low cost of current generation sequencing technologies has led to a dramatic increase in the number of sequenced genomes for species across the tree of life. This increasing volume of data requires tools that can quickly compare multiple whole-genome sequences, millions of base pairs in length, to aid in the study of populations, pan-genomes, and genome evolution.This bookmaks have been created to report new tools for whole genome alignments.</p>
<p>Please report new whole genome alignment tools under comment sections.</p><p>Address of the bookmark: <a href="http://www.cs.utoronto.ca/~brudno/721.full.pdf" rel="nofollow">http://www.cs.utoronto.ca/~brudno/721.full.pdf</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/26409/ucsc-genome-browser-and-blat-software</guid>
	<pubDate>Thu, 18 Feb 2016 03:18:57 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/26409/ucsc-genome-browser-and-blat-software</link>
	<title><![CDATA[UCSC Genome Browser and Blat software !]]></title>
	<description><![CDATA[<p>This directory contains Genome Browser and Blat application binaries built for standalone <br>command-line use on various supported Linux and UNIX platforms. To determine which set of binaries <br>to download, type "uname -a" on the command line to display your machine type. In most cases the <br>usage statement for the application can be viewed by running the binary with no arguments. <br><br>The UCSC Genome Browser and Blat software are free for academic, nonprofit, and personal use. A <br>license is required for commercial download and installation of these binaries, with the exception <br>of items built from the following source code directories, which are freely available for all uses:<br><br>&nbsp;- kent/src/utils (includes big* tools)<br>&nbsp;- kent/src/lib<br>&nbsp;- kent/src/hg/autoSql<br>&nbsp;- kent/src/hg/autoXml<br><br>For information about commercial licensing of the Genome Browser software, see <br>http://genome.ucsc.edu/license/. The Blat and In-Silico PCR software may be commercially<br>licensed through Kent Informatics (http://www.kentinformatics.com).</p>
<p>More at http://hgdownload.cse.ucsc.edu/admin/exe/</p><p>Address of the bookmark: <a href="http://hgdownload.cse.ucsc.edu/admin/exe/" rel="nofollow">http://hgdownload.cse.ucsc.edu/admin/exe/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27090/canu-assembling-large-genomes-with-single-molecule-sequencing-and-locality-sensitive-hashing</guid>
	<pubDate>Tue, 26 Apr 2016 11:38:10 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27090/canu-assembling-large-genomes-with-single-molecule-sequencing-and-locality-sensitive-hashing</link>
	<title><![CDATA[CANU: Assembling Large Genomes with Single-Molecule Sequencing and Locality Sensitive Hashing.]]></title>
	<description><![CDATA[<p>Canu is a fork of the&nbsp;<a href="http://wgs-assembler.sourceforge.net/wiki/index.php?title=Main_Page" title="Celera Assembler">Celera Assembler</a>&nbsp;designed for high-noise single-molecule sequencing (such as the PacBio RSII or Oxford Nanopore MinION). The software is currently alpha level, feel free to use and report issues encountered.</p>
<p>Canu is a hierachical assembly pipeline which runs in four steps:</p>
<ul>
<li>Detect overlaps in high-noise sequences using&nbsp;<a href="https://github.com/marbl/MHAP" title="MHAP">MHAP</a></li>
<li>Generate corrected sequence consensus</li>
<li>Trim corrected sequences</li>
<li>Assemble trimmed corrected sequences</li>
</ul>
<p>Read the&nbsp;<a href="http://canu.readthedocs.org/" title="docs">documentation</a></p>
<p>New release https://github.com/marbl/canu/releases</p><p>Address of the bookmark: <a href="https://github.com/marbl/canu" rel="nofollow">https://github.com/marbl/canu</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27967/linux-command-line-exercises-for-ngs-data-processing</guid>
	<pubDate>Wed, 22 Jun 2016 07:59:39 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27967/linux-command-line-exercises-for-ngs-data-processing</link>
	<title><![CDATA[Linux command line exercises for NGS data processing]]></title>
	<description><![CDATA[<p>The purpose of this tutorial is to introduce students to the frequently used tools for NGS analysis as well as giving experience in writing one-liners. Copy the required files to your current directory, change directory (<code>cd</code>) to the <code>linuxTutorial</code> folder, and do all the processing inside:</p>
<pre><span>[uzi@quince-srv2 ~/]$</span> cp -r /home/opt/MScBioinformatics/linuxTutorial .
<span>[uzi@quince-srv2 ~/]$</span> cd linuxTutorial
<span>[uzi@quince-srv2 ~/linuxTutorial]$</span>
</pre>
<p>I have deliberately chosen <code>Awk</code> in the exercises as it is a language in itself and is used more often to manipulate NGS data as compared to the other command line tools such as <code>grep</code>, <code>sed</code>, <code>perl</code> etc. Furthermore, having a command on <code>awk</code> will make it easier to understand advanced tutorials such as <a href="http://userweb.eng.gla.ac.uk/umer.ijaz/bioinformatics/Illumina_workflow.html">Illumina Amplicons Processing Workflow</a>. <br><br> In <code>Linux</code>, we use a shell that is a program that takes your commands from the keyboard and gives them to the operating system. Most Linux systems utilize Bourne Again SHell (<code>bash</code>), but there are several additional shell programs on a typical Linux system such as <code>ksh</code>, <code>tcsh</code>, and <code>zsh</code>. To see which shell you are using, type</p>
<pre><span>[uzi@quince-srv2 ~/linuxTutorial]$</span> echo $SHELL

<span>/bin/bash
</span></pre><p>Address of the bookmark: <a href="http://userweb.eng.gla.ac.uk/umer.ijaz/bioinformatics/linux.html" rel="nofollow">http://userweb.eng.gla.ac.uk/umer.ijaz/bioinformatics/linux.html</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/29270/blast-ring-image-generator-brig</guid>
	<pubDate>Fri, 30 Sep 2016 09:18:50 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/29270/blast-ring-image-generator-brig</link>
	<title><![CDATA[BLAST Ring Image Generator (BRIG)]]></title>
	<description><![CDATA[<p>BRIG is a free cross-platform (Windows/Mac/Unix) application that can display circular comparisons between a large number of genomes, with a focus on handling genome assembly data. The application is available at: <a href="http://sourceforge.net/projects/brig">http://sourceforge.net/projects/brig</a></p>
<p>If you have any questions or comments, post them on <a href="http://sourceforge.net/tracker/?group_id=328245">one of the trackers</a> on BRIG&rsquo;s SourceForge page: <a href="http://sourceforge.net/tracker/?group_id=328245">http://sourceforge.net/tracker/?group_id=328245</a>.</p>
<p>Features:</p>
<ul>
<li>Images show similarity between a central reference sequence and other sequences as concentric rings.</li>
<li>BRIG will perform all BLAST comparisons and file parsing automatically via a simple GUI.</li>
<li>Contig boundaries and read coverage can be displayed for draft genomes; customized graphs and annotations can be displayed.</li>
<li>Using a user-defined set of genes as input, BRIG can display gene presence, absence, truncation or sequence variation in a set of complete genomes, draft genomes or even raw, unassembled sequence data.</li>
<li>BRIG also accepts SAM-formatted read-mapping files enabling genomic regions present in unassembled sequence data from multiple samples to be compared simultaneously</li>
</ul><p>Address of the bookmark: <a href="http://brig.sourceforge.net/" rel="nofollow">http://brig.sourceforge.net/</a></p>]]></description>
	<dc:creator>Anjana</dc:creator>
</item>

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