Candidates interested in the position should contact me in advance by email to: g.stracquadanio@essex.ac.uk

The deadline for the application is 28/02/2017; info about the application can be found on the Essex CSEE website.

With advances in Cancer Genomics, Mutation Annotation Format (MAF) is being widley accepted and used to store variants detected. The Cancer Genome Atlas Project has seqenced over 30 different cancers with sample size of each cancer type being over 200. The resulting data consisting of genetic variants is stored in the form of Mutation Annotation Format. This package attempts to summarize, analyze, annotate and visualize MAF files in an efficient manner either from TCGA sources or any in-house studies as long as the data is in MAF format. Maftools can also handle ICGC Simple Somatic Mutation format.

maftools is on bioRxiv

Please cite the below if you find this tool useful for you.

Mayakonda, A. and H.P. Koeffler, Maftools: Efficient analysis, visualization and summarization of MAF files from large-scale cohort based cancer studies. bioRxiv, 2016. doi: http://dx.doi.org/10.1101/052662

Address of the bookmark: https://github.com/PoisonAlien/maftools

• optimized to work with vast amount of data and very large file sets (~ 10,000 - 100,000).
• allows the selective retrieval of only the required files (file masks, ls-lR parsing, recursive search, updates)
• has a built-in repository containing the settings for the most common bioinformatics download needs
• built-in wizard for batch post-processing of downloaded files (archive extraction, file conversion, etc.)
• capable of performing multiple download or update tasks simultaneously

BioDownloader has a built-in repository containing the settings for common bioinformatics file-synchronization needs, including the Protein Data Bank (PDB) and National Center for Biotechnology Information (NCBI) databases. It can post-process downloaded files, including archive extraction and file conversions.

http://dunbrack.fccc.edu/BioDownloader/

Address of the bookmark: http://dunbrack.fccc.edu/BioDownloader/

This software distribution includes both the DAGchainer utility and a Java-based graphical interface that allows the inputs and outputs to be navigated and interrogated dynamically.

Address of the bookmark: http://dagchainer.sourceforge.net/

https://github.com/microsoftgenomics

Address of the bookmark: https://github.com/microsoftgenomics

More at https://bioinformaticsonline.com/snippets/view/42919/perl-ipcopen2-module

2. exec””

 syntax: exec "command";

3. Backticks “ or qx//

syntax: `command`;

syntax: qx/command/;

4. IPC::Open3

syntax: $output = open3(\*CHLD_IN, \*CHLD_OUT, \*CHLD_ERR, 'command arg1 arg2', 'optarg',...);

It is very similar to IPC::Open2 with the capability to capture all three file handles of the process, i.e., STDIN, STDOUT, and STDERR. It can also be used with or without the shell. You can read about it more in the documentation: IPC::Open3.

$output = open3(my $o ut, my $in, 'command arg1 arg2');

OR without using the shell

$output = open3(my $out, my $in, 'command', 'arg1', 'arg2');

5.a2p

syntax: a2p [options] [awkscript]

There is a Perl utility known as a2p which translates awk command to Perl. It takes awk script as input and generates a comparable Perl script as the output. Suppose, you want to execute the following awk statement

awk '{$1 = ""; $2 = ""; print}' f1.txt

This statement gives error sometimes even after escaping the variables (\$1, \$2) but by using a2p it can be easily converted to Perl script:

For further information, you can read it’s documentation: a2p

More help at https://bioinformaticsonline.com/snippets/view/42920/perl-script-to-run-awk-inside-perl

6. system()

syntax: system( "command" );

It is also a Perl function (perlfunc) that executes a command and waits for it to get finished first and then resume the Perl script. The return value is the exit status of the command. It can be called in two ways:

system( "command arg1 arg2" );

OR

system( "command", "arg1", "arg2" );

HELP

Here are some useful Perl cheat sheets that can be used as a quick reference guide.-- https://www.pcwdld.com/perl-cheat-sheet

Address of the bookmark: https://sourceforge.net/projects/pb-jelly/

More at http://www.nature.com/articles/srep24175

Address of the bookmark: https://sourceforge.net/projects/sb2nhri/files/MyCC/

Master's degree in bioinformatics, computer science, molecular biology, or related field
One year of experience in analyzing RNA-Seq data
In lieu of a degree, consideration will be given to an equivalent combination of related education and required work experience.
Understanding of molecular biology, biochemistry, and genetics
Proficiency in writing scripts using Perl, Python, Java, or equivalent languages
Proficiency in R and UNIX/LINUX
Knowledge of genomics, alignment, annotation, bioinformatics, concepts of sequence assembly
Highly motivated and detail-oriented
Ability, interest, and curiosity to learn new skills
Must possess strong communication skills to work effectively with users across disciplines
Ability to work independently and as part of a multi-disciplinary team
Strong visual, verbal, and written communication skills
Excellent time organizational skills
Preferred:

Experience writing software or building software pipelines
Experience with oncology-specific public databases including TCGA
Experience with deploying and/or running software on high-performance computational systems
Statistical and experimental design knowledge
Additional Information:

This position is contingent on the availability of funding
Purdue will not sponsor employment authorization for this position
A background check will be required for employment in this position
FLSA: Exempt (Not Eligible For Overtime)
Retirement Eligibility: Defined Contribution Waiting Period
Purdue University is an EOE/AA employer. All individuals, including minorities, women, individuals with disabilities, and veterans are encouraged to apply

More at https://careers.purdue.edu/job/West-Lafayette-Bioinformatician-Purdue-Cancer-Center-IN-47906/686617600/