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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/35920?offset=170</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43447/rna-seq-workflow-gene-level-exploratory-analysis-and-differential-expression</guid>
	<pubDate>Sat, 09 Oct 2021 07:59:23 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43447/rna-seq-workflow-gene-level-exploratory-analysis-and-differential-expression</link>
	<title><![CDATA[RNA-seq workflow: gene-level exploratory analysis and differential expression]]></title>
	<description><![CDATA[<p><span>Here we walk through an end-to-end gene-level RNA-seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were quantified to the reference transcripts, and prepare gene-level count datasets for downstream analysis. We will perform exploratory data analysis (EDA) for quality assessment and to explore the relationship between samples, perform differential gene expression analysis, and visually explore the results.</span></p><p>Address of the bookmark: <a href="http://master.bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html" rel="nofollow">http://master.bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/43999/tools-for-differential-expression-analysis</guid>
	<pubDate>Tue, 08 Nov 2022 03:40:33 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/43999/tools-for-differential-expression-analysis</link>
	<title><![CDATA[Tools for Differential expression analysis]]></title>
	<description><![CDATA[<p><span>apeglm</span>&nbsp;-&nbsp;<a href="https://bioconductor.org/packages/release/bioc/html/apeglm.html" target="_blank">https://bioconductor.org/packages/release/bioc/html/apeglm.html</a></p><p><span>ashr</span>&nbsp;-&nbsp;<a href="https://github.com/stephens999/ashr" target="_blank">https://github.com/stephens999/ashr</a>,&nbsp;<a href="https://cran.r-project.org/web/packages/ashr/index.html" target="_blank">https://cran.r-project.org/web/packages/ashr/index.html</a></p><p><span>consensusDE</span>&nbsp;-&nbsp;<a href="https://bioconductor.org/packages/release/bioc/html/consensusDE.html" target="_blank">https://bioconductor.org/packages/release/bioc/html/consensusDE.html</a></p><p><span>DESeq2</span>&nbsp;-&nbsp;<a href="https://bioconductor.org/packages/release/bioc/html/DESeq2.html" target="_blank">https://bioconductor.org/packages/release/bioc/html/DESeq2.html</a></p><p><span>edgeR</span>&nbsp;-&nbsp;<a href="https://bioconductor.org/packages/release/bioc/html/edgeR.html" target="_blank">https://bioconductor.org/packages/release/bioc/html/edgeR.html</a></p><p><span>limma</span>&nbsp;-&nbsp;<a href="https://kasperdanielhansen.github.io/genbioconductor/html/limma.html" target="_blank">https://kasperdanielhansen.github.io/genbioconductor/html/limma.html</a>&nbsp;&nbsp;<a href="https://bioconductor.org/packages/release/bioc/html/limma.html" target="_blank">https://bioconductor.org/packages/release/bioc/html/limma.html</a></p><p><span>MetaCycle</span>&nbsp;-&nbsp;<a href="https://cran.r-project.org/web/packages/MetaCycle/index.html" target="_blank">https://cran.r-project.org/web/packages/MetaCycle/index.html</a>,&nbsp;<a href="https://github.com/gangwug/MetaCycle" target="_blank">https://github.com/gangwug/MetaCycle</a></p><p><span>RUVSeq</span>&nbsp;-&nbsp;<a href="https://bioconductor.org/packages/release/bioc/html/RUVSeq.html" target="_blank">https://bioconductor.org/packages/release/bioc/html/RUVSeq.html</a></p><p><span>SARTools</span>&nbsp;-&nbsp;<a href="https://github.com/PF2-pasteur-fr/SARTools" target="_blank">https://github.com/PF2-pasteur-fr/SARTools</a></p><p><span>tximport</span>&nbsp;-&nbsp;<a href="https://github.com/mikelove/tximport" target="_blank">https://github.com/mikelove/tximport</a></p><p>&nbsp;</p>]]></description>
	<dc:creator>Abhi</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44561/bactopia-a-flexible-pipeline-for-complete-analysis-of-bacterial-genomes</guid>
	<pubDate>Sat, 08 Jun 2024 16:25:08 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44561/bactopia-a-flexible-pipeline-for-complete-analysis-of-bacterial-genomes</link>
	<title><![CDATA[Bactopia: a flexible pipeline for complete analysis of bacterial genomes]]></title>
	<description><![CDATA[<p>Bactopia is a flexible pipeline for complete analysis of bacterial genomes. The goal of Bactopia is process your data with a broad set of tools, so that you can get to the fun part of analyses quicker!</p>
<p>Bactopia was inspired by&nbsp;<a href="https://staphopia.github.io/">Staphopia</a>, a workflow we (Tim Read and myself) released that is targeted towards&nbsp;<em>Staphylococcus aureus</em>&nbsp;genomes. Using what we learned from Staphopia and user feedback, Bactopia was developed from scratch with usability, portability, and speed in mind from the start.</p>
<p>Bactopia uses&nbsp;<a href="https://www.nextflow.io/">Nextflow</a>&nbsp;to manage the workflow, allowing for support of many types of environments (e.g. cluster or cloud). Bactopia allows for the usage of many public datasets as well as your own datasets to further enhance the analysis of your sequencing. Bactopia only uses software packages available from&nbsp;<a href="https://bioconda.github.io/">Bioconda</a>&nbsp;and&nbsp;<a href="https://conda-forge.org/">Conda-Forge</a>&nbsp;to make installation as simple as possible for&nbsp;<em>all</em>&nbsp;users.</p>
<p>To highlight the use of&nbsp;<a href="https://bactopia.github.io/latest/full-guide/">Bactopia</a>&nbsp;and&nbsp;<a href="https://bactopia.github.io/latest/bactopia-tools/">Bactopia Tools</a>, we performed an analysis of 1,664 public&nbsp;<em>Lactobacillus</em>&nbsp;genomes, focusing on&nbsp;<em>Lactobacillus crispatus</em>, a species that is a common part of the human vaginal microbiome. The results from this analysis are published in mSystems under the title:&nbsp;<em><a href="https://doi.org/10.1128/mSystems.00190-20">Bactopia: a flexible pipeline for complete analysis of bacterial genomes</a></em></p>
<p><a href="https://bactopia.github.io/latest/assets/bactopia-workflow.png"><img src="https://bactopia.github.io/latest/assets/bactopia-workflow.png" alt="Bactopia Workflow" style="border: 0px;"></a></p><p>Address of the bookmark: <a href="https://bactopia.github.io/latest/" rel="nofollow">https://bactopia.github.io/latest/</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/44632/lecturer-uessex-in-evolutionary-biology</guid>
  <pubDate>Tue, 06 Aug 2024 02:41:17 -0500</pubDate>
  <link></link>
  <title><![CDATA[Lecturer @ UEssex in Evolutionary Biology]]></title>
  <description><![CDATA[
<p>The University of Essex, UK, is seeking a Lecturer conducting research in<br />evolutionary biology or a related field. The new Lecturer will join the<br />collegial and supportive environment of the School of Life Sciences,<br />contributing to teaching and leading a research programme, ideally<br />relating to any aspect of evolutionary biology.</p>

<p>Application closing date 16/09/2024</p>

<p>Details of the post and application process can be found at the following<br />url:</p>

<p>https://vacancies.essex.ac.uk/tlive_webrecruitment/wrd/run/ETREC107GF.open?VACANCY_ID=202575WW5Z&amp;WVID=9918109NEm&amp;LANG=USA</p>

<p>Informal enquiries may be made by email to the Head of the School of<br />Life Sciences Prof Terence McGenity at tjmcgen@essex.ac.uk(all formal<br />applications for the post should be made online through the University<br />of Essex website).</p>
]]></description>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/43928/bioinformaticians-in-comparative-and-evolutionary-genomics</guid>
  <pubDate>Tue, 02 Aug 2022 01:22:48 -0500</pubDate>
  <link></link>
  <title><![CDATA[Bioinformaticians in comparative and evolutionary genomics]]></title>
  <description><![CDATA[
<p>NBIS is now looking for a new member to support Swedish research in evolutionary, comparative, and population genomics, with a particular focus on conifer genomics.</p>

<p>Your tasks will consist of:</p>

<p>Advanced bioinformatics analyses within research projects across Sweden, including key involvement in a major research effort in conifer genomics.<br />Development of bioinformatics tools and workflows.<br />Educating other scientists in bioinformatics through collaboration within supported projects, teaching at national courses, and through participating in various networks.<br />Taking part in the continuous development of NBIS/SciLifeLab at a national level</p>

<p>More at https://www.uu.se/en/about-uu/join-us/details/?positionId=518909</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/35432/mummer4-a-fast-and-versatile-genome-alignment-system</guid>
	<pubDate>Sat, 03 Feb 2018 04:59:17 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/35432/mummer4-a-fast-and-versatile-genome-alignment-system</link>
	<title><![CDATA[MUMmer4: A fast and versatile genome alignment system]]></title>
	<description><![CDATA[<p><span>MUMmer4, a substantially improved version of MUMmer that addresses genome size constraints by changing the 32-bit suffix tree data structure at the core of MUMmer to a 48-bit suffix array, and that offers improved speed through parallel processing of input query sequences. With a theoretical limit on the input size of 141Tbp, MUMmer4 can now work with input sequences of any biologically realistic length. We show that as a result of these enhancements, the&nbsp;</span><span>nucmer</span><span>&nbsp;program in MUMmer4 is easily able to handle alignments of large genomes;&nbsp;</span></p><p>Address of the bookmark: <a href="https://mummer4.github.io/" rel="nofollow">https://mummer4.github.io/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41192/kubernetes-open-source-system-for-automating-deployment-scaling-and-management-of-containerized-applications</guid>
	<pubDate>Thu, 20 Feb 2020 00:58:34 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41192/kubernetes-open-source-system-for-automating-deployment-scaling-and-management-of-containerized-applications</link>
	<title><![CDATA[Kubernetes: open-source system for automating deployment, scaling, and management of containerized applications.]]></title>
	<description><![CDATA[<p><span>It groups containers that make up an application into logical units for easy management and discovery. Kubernetes builds upon&nbsp;</span><a href="http://queue.acm.org/detail.cfm?id=2898444" target="_blank">15 years of experience of running production workloads at Google</a><span>, combined with best-of-breed ideas and practices from the community.</span></p><p>Address of the bookmark: <a href="https://kubernetes.io/" rel="nofollow">https://kubernetes.io/</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38745/osprey-network-visualization-system</guid>
	<pubDate>Sun, 20 Jan 2019 05:34:24 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38745/osprey-network-visualization-system</link>
	<title><![CDATA[Osprey: Network Visualization System]]></title>
	<description><![CDATA[<p>Osprey is a software platform for the visualization of complex biological interaction networks. Osprey builds data-rich graphical representations from&nbsp;<a href="http://geneontology.org/" title="GENE ONTOLOGY CONSORTIUM">Gene Ontology (GO)</a>&nbsp;annotated interaction data maintained by the&nbsp;<a href="https://thebiogrid.org/" title="The BioGRID">BioGRID</a>.</p>
<p>Osprey is developed by the&nbsp;<a href="http://www.tyerslab.com/">TyersLab</a>&nbsp;and is a part of the&nbsp;<a href="https://thebiogrid.org/" title="The BioGRID">BioGRID</a>&nbsp;family of software. It utilizes both&nbsp;<a href="https://www.mysql.com/" title="MySQL Database">MySQL</a>&nbsp;and&nbsp;<a href="http://openjdk.java.net/" title="OpenJDK">Java</a>&nbsp;to operate and is compatible with&nbsp;<a href="https://www.microsoft.com/en-us/windows/" title="Microsoft Windows">Windows</a>,&nbsp;<a href="http://www.ubuntu.com/">Linux</a>, and&nbsp;<a href="http://www.apple.com/" title="Apple">Apple</a>&nbsp;operating systems.</p>
<p>These works were published in&nbsp;<strong>Breitkreutz, BJ., Stark, C., Tyers M. "Osprey: A Network Visualization System." Genome Biology 2003 4(3):R22</strong>&nbsp;<a href="http://genomebiology.com/2003/4/3/R22" title="Genome Biology">[Genome Biology]</a>&nbsp;<a href="http://genomebiology.com/content/pdf/gb-2003-4-3-r22.pdf" title="Osprey PDF">[PDF]</a>&nbsp;<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=12620107&amp;dopt=Abstract" title="Pubmed">[PubMed]</a>&nbsp;and supported by the&nbsp;<a href="http://www.nih.gov/" title="NIH">National Institutes of Health</a>,&nbsp;<a href="http://www.cihr-irsc.gc.ca/" title="CIHR">Canadian Institutes of Health Research</a>, and&nbsp;<a href="http://www.genomecanada.ca/en/" title="Genome Canada">Genome Canada</a>.</p><p>Address of the bookmark: <a href="https://osprey.thebiogrid.org/" rel="nofollow">https://osprey.thebiogrid.org/</a></p>]]></description>
	<dc:creator>BioJoker</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/26380/hicdat</guid>
	<pubDate>Fri, 12 Feb 2016 05:23:44 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/26380/hicdat</link>
	<title><![CDATA[HiCdat]]></title>
	<description><![CDATA[<p>HiCdat: a fast and easy-to-use Hi-C data analysis tool</p>
<p>HiCdat is easy-to-use and provides solutions starting from aligned reads up to in-depth analyses. Importantly, HiCdat is focussed on the analysis of larger structural features of chromosomes, their correlation to genomic and epigenomic features, and on comparative studies. It uses simple input and output formats and can therefore easily be integrated into existing workflows or combined with alternative tools.</p>
<p>More at http://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-015-0678-x</p><p>Address of the bookmark: <a href="https://github.com/MWSchmid/HiCdat" rel="nofollow">https://github.com/MWSchmid/HiCdat</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/28809/kissplice</guid>
	<pubDate>Tue, 16 Aug 2016 08:34:19 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/28809/kissplice</link>
	<title><![CDATA[KisSplice]]></title>
	<description><![CDATA[<p>KisSplice is a software that enables to analyse RNA-seq data with or without a reference genome. It is an exact local transcriptome assembler that allows to identify SNPs, indels and alternative splicing events. It can deal with an arbitrary number of biological conditions, and will quantify each variant in each condition. It has been tested on Illumina datasets of up to 1G reads. Its memory consumption is around 5Gb for 100M reads.</p>
<p>KisSplice is not a full-length transcriptome assembler. This means that it will output the variable regions of the transcripts, not reconstruct them entirely.</p>
<p>KisSplice comes as a workflow, with several possible post-treatments meant to facilitate the analysis of the results. The choice of the post-treatment depends on the availability of a reference genome/transcriptome and on the need to perform a differential analysis, as summarised in the following table.</p><p>Address of the bookmark: <a href="http://kissplice.prabi.fr/" rel="nofollow">http://kissplice.prabi.fr/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

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