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<channel>
	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/36456?offset=180</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/36723/hapsembler-an-assembler-for-highly-polymorphic-genomes</guid>
	<pubDate>Tue, 22 May 2018 04:09:53 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/36723/hapsembler-an-assembler-for-highly-polymorphic-genomes</link>
	<title><![CDATA[Hapsembler: An Assembler for Highly Polymorphic Genomes]]></title>
	<description><![CDATA[Hapsembler is a haplotype-specific genome assembly toolkit that is designed for genomes that are rich in SNPs and other types of polymorphism. Hapsembler can be used to assemble reads from a variety of platforms including Illumina and Roche/454. 

http://compbio.cs.toronto.edu/hapsembler/<p>Address of the bookmark: <a href="http://compbio.cs.toronto.edu/hapsembler/" rel="nofollow">http://compbio.cs.toronto.edu/hapsembler/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/37225/installing-trinity</guid>
	<pubDate>Tue, 03 Jul 2018 04:31:15 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/37225/installing-trinity</link>
	<title><![CDATA[Installing Trinity !]]></title>
	<description><![CDATA[
<p>Download this https://github.com/trinityrnaseq/trinityrnaseq/releases/tag/Trinity-v2.6.6</p>

<p>➜ cd trinityrnaseq-Trinity-v2.6.6</p>

<p>➜  trinityrnaseq-Trinity-v2.6.6 git:(master) ✗ make<br />Using gnu compiler for Inchworm and Chrysalis<br />cd Inchworm &amp;&amp; (test -e configure || autoreconf) \<br />                &amp;&amp; sh ./configure --prefix=`pwd`  &amp;&amp; make install<br />checking for a BSD-compatible install... /usr/bin/install -c<br />checking whether build environment is sane... yes<br />checking for a thread-safe mkdir -p... /bin/mkdir -p<br />checking for gawk... gawk<br />checking whether make sets $(MAKE)... yes<br />checking for g++... g++<br />checking whether the C++ compiler works... yes<br />checking for C++ compiler default output file name... a.out<br />checking for suffix of executables... <br />checking whether we are cross compiling... no<br />checking for suffix of object files... o<br />checking whether we are using the GNU C++ compiler... yes<br />checking whether g++ accepts -g... yes<br />checking for style of include used by make... GNU<br />checking dependency style of g++... gcc3<br />checking for library containing cos... none required<br />configure: creating ./config.status<br />config.status: creating Makefile<br />config.status: creating src/Makefile<br />config.status: creating config.h<br />config.status: config.h is unchanged<br />config.status: executing depfiles commands<br />make[1]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm'<br />Making install in src<br />make[2]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm/src'<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT Fasta_entry.o -MD -MP -MF .deps/Fasta_entry.Tpo -c -o Fasta_entry.o Fasta_entry.cpp<br />mv -f .deps/Fasta_entry.Tpo .deps/Fasta_entry.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT IRKE_run.o -MD -MP -MF .deps/IRKE_run.Tpo -c -o IRKE_run.o IRKE_run.cpp<br />mv -f .deps/IRKE_run.Tpo .deps/IRKE_run.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT sequenceUtil.o -MD -MP -MF .deps/sequenceUtil.Tpo -c -o sequenceUtil.o sequenceUtil.cpp<br />mv -f .deps/sequenceUtil.Tpo .deps/sequenceUtil.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT IRKE.o -MD -MP -MF .deps/IRKE.Tpo -c -o IRKE.o IRKE.cpp<br />mv -f .deps/IRKE.Tpo .deps/IRKE.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT KmerCounter.o -MD -MP -MF .deps/KmerCounter.Tpo -c -o KmerCounter.o KmerCounter.cpp<br />mv -f .deps/KmerCounter.Tpo .deps/KmerCounter.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT string_util.o -MD -MP -MF .deps/string_util.Tpo -c -o string_util.o string_util.cpp<br />mv -f .deps/string_util.Tpo .deps/string_util.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT Fasta_reader.o -MD -MP -MF .deps/Fasta_reader.Tpo -c -o Fasta_reader.o Fasta_reader.cpp<br />mv -f .deps/Fasta_reader.Tpo .deps/Fasta_reader.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT stacktrace.o -MD -MP -MF .deps/stacktrace.Tpo -c -o stacktrace.o stacktrace.cpp<br />mv -f .deps/stacktrace.Tpo .deps/stacktrace.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT argProcessor.o -MD -MP -MF .deps/argProcessor.Tpo -c -o argProcessor.o argProcessor.cpp<br />mv -f .deps/argProcessor.Tpo .deps/argProcessor.Po<br />g++ -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2   -o inchworm Fasta_entry.o IRKE_run.o sequenceUtil.o IRKE.o KmerCounter.o string_util.o Fasta_reader.o stacktrace.o argProcessor.o  <br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT cigar_tweaker.o -MD -MP -MF .deps/cigar_tweaker.Tpo -c -o cigar_tweaker.o cigar_tweaker.cpp<br />mv -f .deps/cigar_tweaker.Tpo .deps/cigar_tweaker.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT SAM_entry.o -MD -MP -MF .deps/SAM_entry.Tpo -c -o SAM_entry.o SAM_entry.cpp<br />mv -f .deps/SAM_entry.Tpo .deps/SAM_entry.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT SAM_reader.o -MD -MP -MF .deps/SAM_reader.Tpo -c -o SAM_reader.o SAM_reader.cpp<br />mv -f .deps/SAM_reader.Tpo .deps/SAM_reader.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT Cigar.o -MD -MP -MF .deps/Cigar.Tpo -c -o Cigar.o Cigar.cpp<br />mv -f .deps/Cigar.Tpo .deps/Cigar.Po<br />g++ -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2   -o cigar_tweaker cigar_tweaker.o SAM_entry.o sequenceUtil.o SAM_reader.o Cigar.o string_util.o stacktrace.o Fasta_reader.o Fasta_entry.o  <br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT pull_reads_with_kmers.o -MD -MP -MF .deps/pull_reads_with_kmers.Tpo -c -o pull_reads_with_kmers.o pull_reads_with_kmers.cpp<br />mv -f .deps/pull_reads_with_kmers.Tpo .deps/pull_reads_with_kmers.Po<br />g++ -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2   -o pull_reads_with_kmers pull_reads_with_kmers.o Fasta_reader.o Fasta_entry.o sequenceUtil.o KmerCounter.o string_util.o stacktrace.o argProcessor.o  <br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT FastaToDeBruijn.o -MD -MP -MF .deps/FastaToDeBruijn.Tpo -c -o FastaToDeBruijn.o FastaToDeBruijn.cpp<br />FastaToDeBruijn.cpp: In function ‘void createGraphPerRecord(std::vector &gt;, int, bool, ArgProcessor)’:<br />FastaToDeBruijn.cpp:199:39: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />                 if (seq_region.size() &lt; kmer_length) { continue; }  // can be e<br />                                       ^<br />mv -f .deps/FastaToDeBruijn.Tpo .deps/FastaToDeBruijn.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT DeBruijnGraph.o -MD -MP -MF .deps/DeBruijnGraph.Tpo -c -o DeBruijnGraph.o DeBruijnGraph.cpp<br />mv -f .deps/DeBruijnGraph.Tpo .deps/DeBruijnGraph.Po<br />g++ -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2   -o FastaToDeBruijn FastaToDeBruijn.o argProcessor.o Fasta_reader.o Fasta_entry.o sequenceUtil.o string_util.o stacktrace.o DeBruijnGraph.o  <br />g++ -DHAVE_CONFIG_H -I. -I..    -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2 -MT fastaToKmerCoverageStats.o -MD -MP -MF .deps/fastaToKmerCoverageStats.Tpo -c -o fastaToKmerCoverageStats.o fastaToKmerCoverageStats.cpp<br />fastaToKmerCoverageStats.cpp: In function ‘void populate_kmer_counter_from_reads(KmerCounter&amp;, std::__cxx11::string&amp;)’:<br />fastaToKmerCoverageStats.cpp:226:18: warning: unused variable ‘kmer_length’ [-Wunused-variable]<br />     unsigned int kmer_length = kcounter.get_kmer_length();<br />                  ^<br />mv -f .deps/fastaToKmerCoverageStats.Tpo .deps/fastaToKmerCoverageStats.Po<br />g++ -std=c++0x -pedantic -fopenmp -Wall -Wextra -Wno-deprecated -m64 -g -O2   -o fastaToKmerCoverageStats fastaToKmerCoverageStats.o argProcessor.o Fasta_reader.o Fasta_entry.o sequenceUtil.o string_util.o stacktrace.o KmerCounter.o  <br />make[3]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm/src'<br />test -z "/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm/bin" || /bin/mkdir -p "/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm/bin"<br />  /usr/bin/install -c inchworm cigar_tweaker pull_reads_with_kmers FastaToDeBruijn fastaToKmerCoverageStats '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm/bin'<br />make[3]: Nothing to be done for 'install-data-am'.<br />make[3]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm/src'<br />make[2]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm/src'<br />make[2]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm'<br />make[3]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm'<br />make[3]: Nothing to be done for 'install-exec-am'.<br />make[3]: Nothing to be done for 'install-data-am'.<br />make[3]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm'<br />make[2]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm'<br />make[1]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm'<br />cd Chrysalis &amp;&amp; make UNSUPPORTED=yes <br />make[1]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis'<br />./MakeDepend.cc: In member function ‘bool makefile_builder::dependency::operator&lt;(const makefile_builder::dependency&amp;) const’:<br />./MakeDepend.cc:93:47: warning: suggest parentheses around ‘&amp;&amp;’ within ‘||’ [-Wparentheses]<br />                dependent_ == other.dependent_ &amp;&amp;<br />                                               ^<br />./MakeDepend.cc: In member function ‘bool makefile_builder::subdir_and_target::operator&lt;(const makefile_builder::subdir_and_target&amp;) const’:<br />./MakeDepend.cc:130:41: warning: suggest parentheses around ‘&amp;&amp;’ within ‘||’ [-Wparentheses]<br />                subdir_ == other.subdir_ &amp;&amp;<br />                                         ^<br />./MakeDepend.cc: In function ‘int main(int, char**)’:<br />./MakeDepend.cc:223:20: warning: deprecated conversion from string constant to ‘char*’ [-Wwrite-strings]<br />   char * options = "Ad:g:u:fh";<br />                    ^<br />./MakeDepend.cc: In member function ‘void makefile_builder::DumpDependencies(const string&amp;)’:<br />./MakeDepend.cc:491:46: warning: suggest parentheses around ‘&amp;&amp;’ within ‘||’ [-Wparentheses]<br />       if ( ! this-&gt;FileIsSource( dependent ) &amp;&amp; ! this-&gt;FileIsHeader( dependent<br />                                              ^<br />./MakeDepend.cc:493:44: warning: suggest parentheses around ‘&amp;&amp;’ within ‘||’ [-Wparentheses]<br />            this-&gt;FileIsHeader( dependent ) &amp;&amp; this-&gt;FileIsSource( provider ) ) <br />                                            ^<br />./MakeDepend.cc: In member function ‘bool makefile_builder::FileIsHeader(const string&amp;) const’:<br />./MakeDepend.cc:1339:32: warning: suggest parentheses around ‘&amp;&amp;’ within ‘||’ [-Wparentheses]<br />   return ( filename.size() &gt; 2 &amp;&amp;<br />                                ^<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:04 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./aligns/KmerAlignCore.cc -o obj/aligns/KmerAlignCore.o<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:06 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/AACodons.cc -o obj/analysis/AACodons.o<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:09 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/BreakTransByPairs.cc -o obj/analysis/BreakTransByPairs.o<br />./analysis/BreakTransByPairs.cc: In function ‘int main(int, char**)’:<br />./analysis/BreakTransByPairs.cc:105:14: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />   for (i=0; i &gt; DeBruijnGraph::get_candidate_weldmers(kmer_int_type_t, int)’:<br />./analysis/DeBruijnGraph.cc:473:23: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />     for (int i = 0; i &lt; left_extensions.size(); i++) {<br />                       ^<br />./analysis/DeBruijnGraph.cc:476:27: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />         for (int j = 0; j &lt; right_extensions.size(); j++) {<br />                           ^<br />./analysis/DeBruijnGraph.cc: In member function ‘void DeBruijnGraph::recursively_construct_kmer_extensions(kmer_int_type_t, std::vector&amp;, std::vector &gt;&amp;, char, std::map&amp;, int)’:<br />./analysis/DeBruijnGraph.cc:513:23: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />     for (int i = 0; i &lt; adjacent_kmers.size(); i++) {<br />                       ^<br />./analysis/DeBruijnGraph.cc:527:41: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />         if (kmer_extension_chars.size() == flank_extension_length) {<br />                                         ^<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:30 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/sequenceUtil.cc -o obj/analysis/sequenceUtil.o<br />./analysis/sequenceUtil.cc: In function ‘bool contains_non_gatc(std::__cxx11::string)’:<br />./analysis/sequenceUtil.cc:33:20: warning: array subscript has type ‘char’ [-Wchar-subscripts]<br />  if (_base_to_int[c] &gt; 3)<br />                    ^<br />./analysis/sequenceUtil.cc: In function ‘kmer_int_type_t kmer_to_intval(std::__cxx11::string)’:<br />./analysis/sequenceUtil.cc:264:26: warning: array subscript has type ‘char’ [-Wchar-subscripts]<br />  int val = _base_to_int[c];<br />                          ^<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:31 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/stacktrace.cc -o obj/analysis/stacktrace.o<br />/bin/rm -f lib_BubbleUpClustering_temp.a<br />ar -qc  lib_BubbleUpClustering_temp.a obj/aligns/KmerAlignCore.o obj/analysis/AACodons.o obj/analysis/BubbleUpClustering.o obj/analysis/DNAVector.o obj/analysis/DeBruijnGraph.o obj/analysis/KmerTable.o obj/analysis/NonRedKmerTable.o obj/analysis/sequenceUtil.o obj/analysis/stacktrace.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./BubbleUpClustering<br />g++ -ggdb3    -fopenmp  -o ./BubbleUpClustering  obj/analysis/BubbleUpClustering.o -L. -lm -pthread     -l_BubbleUpClustering_temp<br />/bin/rm lib_BubbleUpClustering_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:32 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/Chrysalis.cc -o obj/analysis/Chrysalis.o<br />./analysis/Chrysalis.cc: In function ‘int main(int, char**)’:<br />./analysis/Chrysalis.cc:384:28: warning: ignoring return value of ‘int system(const char*)’, declared with attribute warn_unused_result [-Wunused-result]<br />     system(command.c_str());<br />                            ^<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:35 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/TranscriptomeGraph.cc -o obj/analysis/TranscriptomeGraph.o<br />./analysis/TranscriptomeGraph.cc: In function ‘int TranscriptomeGraph(vecDNAVector&amp;, FILE*, int, bool)’:<br />./analysis/TranscriptomeGraph.cc:668:20: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />         for (i=0; i&lt;=d.isize()-k; i++) {<br />                    ^<br />/bin/rm -f lib_Chrysalis_temp.a<br />ar -qc  lib_Chrysalis_temp.a obj/aligns/KmerAlignCore.o obj/analysis/AACodons.o obj/analysis/Chrysalis.o obj/analysis/DNAVector.o obj/analysis/TranscriptomeGraph.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./Chrysalis<br />g++ -ggdb3    -fopenmp  -o ./Chrysalis  obj/analysis/Chrysalis.o -L. -lm -pthread     -l_Chrysalis_temp<br />/bin/rm lib_Chrysalis_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:39 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/CreateIwormFastaBundle.cc -o obj/analysis/CreateIwormFastaBundle.o<br />/bin/rm -f lib_CreateIwormFastaBundle_temp.a<br />ar -qc  lib_CreateIwormFastaBundle_temp.a obj/analysis/AACodons.o obj/analysis/CreateIwormFastaBundle.o obj/analysis/DNAVector.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./CreateIwormFastaBundle<br />g++ -ggdb3    -fopenmp  -o ./CreateIwormFastaBundle  obj/analysis/CreateIwormFastaBundle.o -L. -lm -pthread     -l_CreateIwormFastaBundle_temp<br />/bin/rm lib_CreateIwormFastaBundle_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:42 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/GraphFromFasta.cc -o obj/analysis/GraphFromFasta.o<br />./analysis/GraphFromFasta.cc: In function ‘bool SimpleHalves(const DNAVector&amp;)’:<br />./analysis/GraphFromFasta.cc:255:15: warning: suggest parentheses around ‘&amp;&amp;’ within ‘||’ [-Wparentheses]<br />               &amp;&amp; <br />               ^<br />./analysis/GraphFromFasta.cc: In function ‘void report_iworm_graph(std::map&lt;int, Pool&gt;&amp;, std::map&amp;)’:<br />./analysis/GraphFromFasta.cc:631:31: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />             for (int j = 0; j &lt; adjacent_nodes.size(); j++) {<br />                               ^<br />./analysis/GraphFromFasta.cc: In function ‘int main(int, char**)’:<br />./analysis/GraphFromFasta.cc:1164:30: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />         for (i=0; i bubble_up_cluster_growth(std::map&amp;, std::map&amp;)’:<br />./GraphFromFasta_MPI.cc:706:23: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />     for (int i = 0; i &lt; pool_vec.size(); i++) {<br />                       ^<br />./GraphFromFasta_MPI.cc:727:27: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />         for (int i = 0; i &lt; pool_vec.size(); i++) {<br />                           ^<br />./GraphFromFasta_MPI.cc:834:23: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />     for (int i = 0; i &lt; pool_vec.size(); i++) {<br />                       ^<br />./GraphFromFasta_MPI.cc: In function ‘void add_unclustered_iworm_contigs(svec&amp;, vecDNAVector&amp;)’:<br />./GraphFromFasta_MPI.cc:1144:30: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />         for (size_t i = 0; i &lt; p.size(); i++) {<br />                              ^<br />./GraphFromFasta_MPI.cc: In function ‘int main(int, char**)’:<br />./GraphFromFasta_MPI.cc:1453:60: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />             if (iworm_counter % 1000 == 0 || iworm_counter == dna.size()-1) {<br />                                                            ^<br />./GraphFromFasta_MPI.cc:1458:24: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />             for (j=0; j&lt;=d.isize()-k; j++) {<br />                        ^<br />./GraphFromFasta_MPI.cc:1479:27: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />                     if (c == i)<br />                           ^<br />./GraphFromFasta_MPI.cc:1503:27: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />                     if (c == i)<br />                           ^<br />./GraphFromFasta_MPI.cc:2235:24: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />             for (j=0; j&lt;=d.isize()-k; j++) {<br />                        ^<br />./GraphFromFasta_MPI.cc:2264:27: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />                     if (c == i) {<br />                           ^<br />./GraphFromFasta_MPI.cc:2359:27: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />                     if (c == i) {<br />                           ^<br />./GraphFromFasta_MPI.cc:2475:16: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />     for (i=0; i&lt;clustered_pools.isize(); i++) {<br />                ^<br />./GraphFromFasta_MPI.cc:2483:30: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />         for (size_t j = 0; j &lt; p.size(); j++) {<br />                              ^<br />./GraphFromFasta_MPI.cc:2496:30: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />         for (size_t j = 0; j &lt; p.size(); j++) {<br />                              ^<br />/bin/rm -f lib_GraphFromFasta_MPI_temp.a<br />ar -qc  lib_GraphFromFasta_MPI_temp.a obj/GraphFromFasta_MPI.o obj/aligns/KmerAlignCore.o obj/analysis/AACodons.o obj/analysis/DNAVector.o obj/analysis/DeBruijnGraph.o obj/analysis/KmerTable.o obj/analysis/NonRedKmerTable.o obj/analysis/sequenceUtil.o obj/analysis/stacktrace.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./GraphFromFasta_MPI<br />g++ -ggdb3    -fopenmp  -o ./GraphFromFasta_MPI  obj/GraphFromFasta_MPI.o -L. -lm -pthread     -l_GraphFromFasta_MPI_temp<br />/bin/rm lib_GraphFromFasta_MPI_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:53 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/IsoformAugment.cc -o obj/analysis/IsoformAugment.o<br />./analysis/IsoformAugment.cc: In function ‘int main(int, char**)’:<br />./analysis/IsoformAugment.cc:87:16: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />     for (j=0; j&lt;matchesLeft.isize(); j++) {<br />                ^<br />./analysis/IsoformAugment.cc:122:16: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />     for (j=0; j&lt;matches.isize(); j++) {<br />                ^<br />/bin/rm -f lib_IsoformAugment_temp.a<br />ar -qc  lib_IsoformAugment_temp.a obj/aligns/KmerAlignCore.o obj/analysis/AACodons.o obj/analysis/DNAVector.o obj/analysis/IsoformAugment.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./IsoformAugment<br />g++ -ggdb3    -fopenmp  -o ./IsoformAugment  obj/analysis/IsoformAugment.o -L. -lm -pthread     -l_IsoformAugment_temp<br />/bin/rm lib_IsoformAugment_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:27:56 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/JoinTransByPairs.cc -o obj/analysis/JoinTransByPairs.o<br />./analysis/JoinTransByPairs.cc: In function ‘int main(int, char**)’:<br />./analysis/JoinTransByPairs.cc:293:14: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />   for (i=0; i&lt;dna.size(); i++) {<br />              ^<br />./analysis/JoinTransByPairs.cc:341:14: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />   for (i=0; i&lt;dna.size(); i++) {<br />              ^<br />/bin/rm -f lib_JoinTransByPairs_temp.a<br />ar -qc  lib_JoinTransByPairs_temp.a obj/aligns/KmerAlignCore.o obj/analysis/AACodons.o obj/analysis/DNAVector.o obj/analysis/JoinTransByPairs.o obj/analysis/KmerTable.o obj/analysis/NonRedKmerTable.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./JoinTransByPairs<br />g++ -ggdb3    -fopenmp  -o ./JoinTransByPairs  obj/analysis/JoinTransByPairs.o -L. -lm -pthread     -l_JoinTransByPairs_temp<br />/bin/rm lib_JoinTransByPairs_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:28:00 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/QuantifyGraph.cc -o obj/analysis/QuantifyGraph.o<br />/bin/rm -f lib_QuantifyGraph_temp.a<br />ar -qc  lib_QuantifyGraph_temp.a obj/aligns/KmerAlignCore.o obj/analysis/AACodons.o obj/analysis/DNAVector.o obj/analysis/KmerTable.o obj/analysis/NonRedKmerTable.o obj/analysis/QuantifyGraph.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./QuantifyGraph<br />g++ -ggdb3    -fopenmp  -o ./QuantifyGraph  obj/analysis/QuantifyGraph.o -L. -lm -pthread     -l_QuantifyGraph_temp<br />/bin/rm lib_QuantifyGraph_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:28:03 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/ReadsToTranscripts.cc -o obj/analysis/ReadsToTranscripts.o<br />/bin/rm -f lib_ReadsToTranscripts_temp.a<br />ar -qc  lib_ReadsToTranscripts_temp.a obj/analysis/AACodons.o obj/analysis/DNAVector.o obj/analysis/NonRedKmerTable.o obj/analysis/ReadsToTranscripts.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./ReadsToTranscripts<br />g++ -ggdb3    -fopenmp  -o ./ReadsToTranscripts  obj/analysis/ReadsToTranscripts.o -L. -lm -pthread     -l_ReadsToTranscripts_temp<br />/bin/rm lib_ReadsToTranscripts_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:28:09 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./ReadsToTranscripts_MPI.cc -o obj/ReadsToTranscripts_MPI.o<br />/bin/rm -f lib_ReadsToTranscripts_MPI_temp.a<br />ar -qc  lib_ReadsToTranscripts_MPI_temp.a obj/ReadsToTranscripts_MPI.o obj/analysis/AACodons.o obj/analysis/DNAVector.o obj/analysis/NonRedKmerTable.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./ReadsToTranscripts_MPI<br />g++ -ggdb3    -fopenmp  -o ./ReadsToTranscripts_MPI  obj/ReadsToTranscripts_MPI.o -L. -lm -pthread     -l_ReadsToTranscripts_MPI_temp<br />/bin/rm lib_ReadsToTranscripts_MPI_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:28:14 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./ReadsToTranscripts_MPI_chang.cc -o obj/ReadsToTranscripts_MPI_chang.o<br />/bin/rm -f lib_ReadsToTranscripts_MPI_chang_temp.a<br />ar -qc  lib_ReadsToTranscripts_MPI_chang_temp.a obj/ReadsToTranscripts_MPI_chang.o obj/analysis/AACodons.o obj/analysis/DNAVector.o obj/analysis/NonRedKmerTable.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./ReadsToTranscripts_MPI_chang<br />g++ -ggdb3    -fopenmp  -o ./ReadsToTranscripts_MPI_chang  obj/ReadsToTranscripts_MPI_chang.o -L. -lm -pthread     -l_ReadsToTranscripts_MPI_chang_temp<br />/bin/rm lib_ReadsToTranscripts_MPI_chang_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:28:18 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/RunButterfly.cc -o obj/analysis/RunButterfly.o<br />./analysis/RunButterfly.cc: In member function ‘void JobControl::ClearFiles()’:<br />./analysis/RunButterfly.cc:107:25: warning: ignoring return value of ‘int system(const char*)’, declared with attribute warn_unused_result [-Wunused-result]<br />       system(rm.c_str());<br />                         ^<br />./analysis/RunButterfly.cc: In member function ‘void JobControl::Submit()’:<br />./analysis/RunButterfly.cc:77:27: warning: ignoring return value of ‘int system(const char*)’, declared with attribute warn_unused_result [-Wunused-result]<br />    system(theCmmd.c_str());<br />                           ^<br />./analysis/RunButterfly.cc:85:27: warning: ignoring return value of ‘int system(const char*)’, declared with attribute warn_unused_result [-Wunused-result]<br />    system(theCmmd.c_str());   <br />                           ^<br />/bin/rm -f lib_RunButterfly_temp.a<br />ar -qc  lib_RunButterfly_temp.a obj/analysis/RunButterfly.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./RunButterfly<br />g++ -ggdb3    -fopenmp  -o ./RunButterfly  obj/analysis/RunButterfly.o -L. -lm -pthread     -l_RunButterfly_temp<br />/bin/rm lib_RunButterfly_temp.a<br />g++  -W -Wall -Wno-unused -Wno-deprecated -ansi -pedantic -Wno-long-long -fno-nonansi-builtins -Wctor-dtor-privacy -Wsign-promo -Woverloaded-virtual -Wendif-labels  -O3  -ggdb3 -DMAKE_DATE='"Die Jul  3 11:28:23 CEST 2018  "' -DMAKE_OS_RELEASE='"4.4.0-128-generic"' -DMAKE_RELEASE='"3.0"' -DNEW_MAKEFILE -imacros system/BigFileDefines.h -pthread  -ftemplate-depth-30 -fno-strict-aliasing -mieee-fp -iquote . -ISpines   -fopenmp        -c ./analysis/TranscriptomeFromVaryK.cc -o obj/analysis/TranscriptomeFromVaryK.o<br />./analysis/TranscriptomeFromVaryK.cc: In function ‘int main(int, char**)’:<br />./analysis/TranscriptomeFromVaryK.cc:216:16: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />     for (j=0; j&lt;=d.lsize()-k; j++) {<br />                ^<br />./analysis/TranscriptomeFromVaryK.cc:218:22: warning: comparison between signed and unsigned integer expressions [-Wsign-compare]<br />       for (int x=j; x&lt;j+k; x++) {<br />                      ^<br />/bin/rm -f lib_TranscriptomeFromVaryK_temp.a<br />ar -qc  lib_TranscriptomeFromVaryK_temp.a obj/aligns/KmerAlignCore.o obj/analysis/AACodons.o obj/analysis/DNAVector.o obj/analysis/KmerTable.o obj/analysis/NonRedKmerTable.o obj/analysis/TranscriptomeFromVaryK.o obj/base/ErrorHandling.o obj/base/FileParser.o obj/base/StringUtil.o obj/util/mutil.o<br />./checkLock ./TranscriptomeFromVaryK<br />g++ -ggdb3    -fopenmp  -o ./TranscriptomeFromVaryK  obj/analysis/TranscriptomeFromVaryK.o -L. -lm -pthread     -l_TranscriptomeFromVaryK_temp<br />/bin/rm lib_TranscriptomeFromVaryK_temp.a<br />make[1]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis'<br />cd trinity-plugins &amp;&amp; make trinity_essentials<br />make[1]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins'<br />tar xvf seqtk-trinity.v0.0.2.tar.gz<br />seqtk-trinity-0.0.2/<br />seqtk-trinity-0.0.2/.gitignore<br />seqtk-trinity-0.0.2/LICENSE<br />seqtk-trinity-0.0.2/Makefile<br />seqtk-trinity-0.0.2/README.md<br />seqtk-trinity-0.0.2/khash.h<br />seqtk-trinity-0.0.2/kseq.h<br />seqtk-trinity-0.0.2/seqtk.c<br />seqtk-trinity-0.0.2/testing/<br />seqtk-trinity-0.0.2/testing/newformat_1.fq<br />seqtk-trinity-0.0.2/testing/newformat_2.fq<br />seqtk-trinity-0.0.2/testing/oldformat_1.fq<br />seqtk-trinity-0.0.2/testing/oldformat_2.fq<br />cd seqtk-trinity-0.0.2 &amp;&amp; make<br />make[2]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/seqtk-trinity-0.0.2'<br />gcc -g -Wall -O2 -Wno-unused-function seqtk.c -o seqtk-trinity -lz -lm<br />seqtk.c: In function ‘stk_comp’:<br />seqtk.c:444:16: warning: variable ‘lc’ set but not used [-Wunused-but-set-variable]<br />    int la, lb, lc, na, nb, nc, cnt[11];<br />                ^<br />make[2]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/seqtk-trinity-0.0.2'<br />mv seqtk-trinity-0.0.2/seqtk-trinity ./BIN/.<br />tar -zxvf "ParaFly-0.1.0".tar.gz &amp;&amp; \<br />cd "ParaFly-0.1.0" &amp;&amp; sh ./configure --prefix=`pwd` &amp;&amp; make install &amp;&amp; \<br />cp bin/ParaFly ../BIN/<br />ParaFly-0.1.0/<br />ParaFly-0.1.0/LICENSE<br />ParaFly-0.1.0/Makefile<br />ParaFly-0.1.0/Makefile.am<br />ParaFly-0.1.0/Makefile.in<br />ParaFly-0.1.0/README.md<br />ParaFly-0.1.0/aclocal.m4<br />ParaFly-0.1.0/bin/<br />ParaFly-0.1.0/bin/ParaFly<br />ParaFly-0.1.0/config.h<br />ParaFly-0.1.0/config.h.in<br />ParaFly-0.1.0/config.log<br />ParaFly-0.1.0/config.status<br />ParaFly-0.1.0/configure<br />ParaFly-0.1.0/configure.ac<br />ParaFly-0.1.0/depcomp<br />ParaFly-0.1.0/install-sh<br />ParaFly-0.1.0/missing<br />ParaFly-0.1.0/src/<br />ParaFly-0.1.0/src/.deps/<br />ParaFly-0.1.0/src/.deps/ParaFly.Po<br />ParaFly-0.1.0/src/.deps/argProcessor.Po<br />ParaFly-0.1.0/src/Makefile<br />ParaFly-0.1.0/src/Makefile.am<br />ParaFly-0.1.0/src/Makefile.in<br />ParaFly-0.1.0/src/ParaFly.cpp<br />ParaFly-0.1.0/src/argProcessor.cpp<br />ParaFly-0.1.0/src/argProcessor.hpp<br />ParaFly-0.1.0/stamp-h1<br />ParaFly-0.1.0/test/<br />ParaFly-0.1.0/test/Makefile<br />ParaFly-0.1.0/test/cmds.txt<br />ParaFly-0.1.0/test/rand_exit.pl<br />checking for a BSD-compatible install... /usr/bin/install -c<br />checking whether build environment is sane... yes<br />checking for a thread-safe mkdir -p... /bin/mkdir -p<br />checking for gawk... gawk<br />checking whether make sets $(MAKE)... yes<br />checking for g++... g++<br />checking for C++ compiler default output file name... a.out<br />checking whether the C++ compiler works... yes<br />checking whether we are cross compiling... no<br />checking for suffix of executables... <br />checking for suffix of object files... o<br />checking whether we are using the GNU C++ compiler... yes<br />checking whether g++ accepts -g... yes<br />checking for style of include used by make... GNU<br />checking dependency style of g++... gcc3<br />checking for library containing cos... none required<br />configure: creating ./config.status<br />config.status: creating Makefile<br />config.status: creating src/Makefile<br />config.status: creating config.h<br />config.status: config.h is unchanged<br />config.status: executing depfiles commands<br />make[2]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0'<br />Making install in src<br />make[3]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0/src'<br />g++ -DHAVE_CONFIG_H -I. -I..    -pedantic -fopenmp -Wall -Wextra -Wno-long-long -Wno-deprecated -m64 -g -O2 -MT ParaFly.o -MD -MP -MF .deps/ParaFly.Tpo -c -o ParaFly.o ParaFly.cpp<br />mv -f .deps/ParaFly.Tpo .deps/ParaFly.Po<br />g++ -DHAVE_CONFIG_H -I. -I..    -pedantic -fopenmp -Wall -Wextra -Wno-long-long -Wno-deprecated -m64 -g -O2 -MT argProcessor.o -MD -MP -MF .deps/argProcessor.Tpo -c -o argProcessor.o argProcessor.cpp<br />mv -f .deps/argProcessor.Tpo .deps/argProcessor.Po<br />g++ -pedantic -fopenmp -Wall -Wextra -Wno-long-long -Wno-deprecated -m64 -g -O2   -o ParaFly ParaFly.o argProcessor.o  <br />make[4]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0/src'<br />test -z "/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0/bin" || /bin/mkdir -p "/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0/bin"<br />  /usr/bin/install -c ParaFly '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0/bin'<br />make[4]: Nothing to be done for 'install-data-am'.<br />make[4]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0/src'<br />make[3]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0/src'<br />make[3]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0'<br />make[4]: Entering directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0'<br />make[4]: Nothing to be done for 'install-exec-am'.<br />make[4]: Nothing to be done for 'install-data-am'.<br />make[4]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0'<br />make[3]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0'<br />make[2]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/ParaFly-0.1.0'<br />ln -sf Trimmomatic-0.36 Trimmomatic<br />make[1]: Leaving directory '/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins'<br />sh ./util/support_scripts/trinity_install_tests.sh<br />~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</p>

<p>Performing Unit Tests of Build<br /> <br />~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br />Inchworm:                has been Installed Properly<br />Chrysalis:               has been Installed Properly<br />QuantifyGraph:           has been Installed Properly<br />GraphFromFasta:          has been Installed Properly<br />ReadsToTranscripts:      has been Installed Properly<br />parafly:                 has been Installed Properly<br />➜  trinityrnaseq-Trinity-v2.6.6 git:(master) ✗<br />➜  trinityrnaseq-Trinity-v2.6.6 git:(master) ✗ ./Trinity</p>

<p>###############################################################################<br />#</p>

<p>     ______  ____   ____  ____   ____  ______  __ __<br />    |      ||    \ |    ||    \ |    ||      ||  |  |<br />    |      ||  D  ) |  | |  _  | |  | |      ||  |  |<br />    |_|  |_||    /  |  | |  |  | |  | |_|  |_||  ~  |<br />      |  |  |    \  |  | |  |  | |  |   |  |  |___, |<br />      |  |  |  .  \ |  | |  |  | |  |   |  |  |     |<br />      |__|  |__|\_||____||__|__||____|  |__|  |____/</p>

<p>#<br />#<br /># Required:<br />#<br />#  --seqType &lt;string&gt;      :type of reads: ('fa' or 'fq')<br />#<br />#  --max_memory       :suggested max memory to use by Trinity where limiting can be enabled. (jellyfish, sorting, etc)<br />#                            provided in Gb of RAM, ie.  '--max_memory 10G'<br />#<br />#  If paired reads:<br />#      --left      :left reads, one or more file names (separated by commas, no spaces)<br />#      --right     :right reads, one or more file names (separated by commas, no spaces)<br />#<br />#  Or, if unpaired reads:<br />#      --single    :single reads, one or more file names, comma-delimited (note, if single file contains pairs, can use flag: --run_as_paired )<br />#<br />#  Or,<br />#      --samples_file          tab-delimited text file indicating biological replicate relationships.<br />#                                   ex.<br />#                                        cond_A    cond_A_rep1    A_rep1_left.fq    A_rep1_right.fq<br />#                                        cond_A    cond_A_rep2    A_rep2_left.fq    A_rep2_right.fq<br />#                                        cond_B    cond_B_rep1    B_rep1_left.fq    B_rep1_right.fq<br />#                                        cond_B    cond_B_rep2    B_rep2_left.fq    B_rep2_right.fq<br />#<br />#                      # if single-end instead of paired-end, then leave the 4th column above empty.<br />#<br />####################################<br />##  Misc:  #########################<br />#<br />#  --SS_lib_type           :Strand-specific RNA-Seq read orientation.<br />#                                   if paired: RF or FR,<br />#                                   if single: F or R.   (dUTP method = RF)<br />#                                   See web documentation.<br />#<br />#  --CPU                      :number of CPUs to use, default: 2<br />#  --min_contig_length        :minimum assembled contig length to report<br />#                                   (def=200)<br />#<br />#  --long_reads            :fasta file containing error-corrected or circular consensus (CCS) pac bio reads<br />#                                   (** note: experimental parameter **, this functionality continues to be under development)<br />#<br />#  --genome_guided_bam     :genome guided mode, provide path to coordinate-sorted bam file.<br />#                                   (see genome-guided param section under --show_full_usage_info)<br />#<br />#  --jaccard_clip                  :option, set if you have paired reads and<br />#                                   you expect high gene density with UTR<br />#                                   overlap (use FASTQ input file format<br />#                                   for reads).<br />#                                   (note: jaccard_clip is an expensive<br />#                                   operation, so avoid using it unless<br />#                                   necessary due to finding excessive fusion<br />#                                   transcripts w/o it.)<br />#<br />#  --trimmomatic                   :run Trimmomatic to quality trim reads<br />#                                        see '--quality_trimming_params' under full usage info for tailored settings.<br />#                                  <br />#<br />#  --no_normalize_reads            :Do *not* run in silico normalization of reads. Defaults to max. read coverage of 50.<br />#                                       see '--normalize_max_read_cov' under full usage info for tailored settings.<br />#                                       (note, as of Sept 21, 2016, normalization is on by default)<br />#     <br />#  --no_distributed_trinity_exec   :do not run Trinity phase 2 (assembly of partitioned reads), and stop after generating command list.<br />#<br />#<br />#  --output                :name of directory for output (will be<br />#                                   created if it doesn't already exist)<br />#                                   default( your current working directory: "/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir" <br />#                                    note: must include 'trinity' in the name as a safety precaution! )<br />#             <br />#  --workdir               :where Trinity phase-2 assembly computation takes place (defaults to --output setting).<br />#                                  (can set this to a node-local drive or RAM disk)     <br />#  <br />#  --full_cleanup                  :only retain the Trinity fasta file, rename as ${output_dir}.Trinity.fasta<br />#<br />#  --cite                          :show the Trinity literature citation<br />#<br />#  --verbose                       :provide additional job status info during the run.<br />#<br />#  --version                       :reports Trinity version (Trinity-v2.6.6) and exits.<br />#<br />#  --show_full_usage_info          :show the many many more options available for running Trinity (expert usage).<br />#<br />#<br />###############################################################################<br />#<br />#  *Note, a typical Trinity command might be:<br />#<br />#        Trinity --seqType fq --max_memory 50G --left reads_1.fq  --right reads_2.fq --CPU 6<br />#<br />#<br />#    and for Genome-guided Trinity:<br />#<br />#        Trinity --genome_guided_bam rnaseq_alignments.csorted.bam --max_memory 50G<br />#                --genome_guided_max_intron 10000 --CPU 6<br />#<br />#     see: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/sample_data/test_Trinity_Assembly/<br />#          for sample data and 'runMe.sh' for example Trinity execution<br />#<br />#     For more details, visit: http://trinityrnaseq.github.io<br />#<br />###############################################################################</p>
]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43652/peregrine-shimmer-genome-assembly-toolkit</guid>
	<pubDate>Thu, 16 Dec 2021 02:50:19 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43652/peregrine-shimmer-genome-assembly-toolkit</link>
	<title><![CDATA[Peregrine &amp; SHIMMER Genome Assembly Toolkit]]></title>
	<description><![CDATA[<p><span>Peregrine is a fast genome assembler for accurate long reads (length &gt; 10kb, accuracy &gt; 99%). It can assemble a human genome from 30x reads within 20 cpu hours from reads to polished consensus. It uses Sparse HIereachical MimiMizER (SHIMMER) for fast read-to-read overlaping without quadratic comparisions used in other OLC assemblers.</span></p><p>Address of the bookmark: <a href="https://github.com/cschin/Peregrine" rel="nofollow">https://github.com/cschin/Peregrine</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44474/claw-chloroplast-long-read-assembly-workflow</guid>
	<pubDate>Wed, 21 Feb 2024 12:37:46 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44474/claw-chloroplast-long-read-assembly-workflow</link>
	<title><![CDATA[CLAW: Chloroplast Long-read Assembly Workflow]]></title>
	<description><![CDATA[<p dir="auto">CLAW (Chloroplast Long-read Assembly Workflow) is an mostly-automated Snakemake-based workflow for the assembly of chloroplast genomes. CLAW uses chloroplast long-reads, which are baited out of larger read libraries (e.g., an Oxford Nanopore Technologies MinION read library derived from photosynthetic tissue), for assembly with Flye and/or Unicycler. CLAW was designed with the novice bioinformatician in mind - it is easy to install and easy to use, requiring only minimal user input.</p><p>Address of the bookmark: <a href="https://github.com/aaronphillips7493/CLAW" rel="nofollow">https://github.com/aaronphillips7493/CLAW</a></p>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/2423/cancers-origins-revealed</guid>
	<pubDate>Thu, 15 Aug 2013 13:06:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/2423/cancers-origins-revealed</link>
	<title><![CDATA[Cancer's origins revealed]]></title>
	<description><![CDATA[<p>Researchers have provided the first comprehensive compendium of mutational processes that drive tumour development. Together, these mutational processes explain most mutations found in 30 of the most common cancer types. This new understanding of cancer development could help to treat and prevent a wide-range of cancers.<br /><br />More at &gt;&gt; http://www.sanger.ac.uk/about/press/2013/130814.html</p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/videolist/watch/3918/the-human-genome-project-video-3d-animation-introduction-low</guid>
	<pubDate>Sat, 24 Aug 2013 19:01:19 -0500</pubDate>
	<link>https://bioinformaticsonline.com/videolist/watch/3918/the-human-genome-project-video-3d-animation-introduction-low</link>
	<title><![CDATA[The Human Genome Project Video   3D Animation Introduction Low)]]></title>
	<description><![CDATA[<iframe width="" height="" src="https://www.youtube-nocookie.com/embed/YxoQFSBwyms" frameborder="0" allowfullscreen></iframe>]]></description>
	
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/videolist/watch/4762/how-dna-is-packaged-advanced</guid>
	<pubDate>Mon, 23 Sep 2013 18:08:34 -0500</pubDate>
	<link>https://bioinformaticsonline.com/videolist/watch/4762/how-dna-is-packaged-advanced</link>
	<title><![CDATA[How DNA is Packaged (Advanced)]]></title>
	<description><![CDATA[<iframe width="" height="" src="https://www.youtube-nocookie.com/embed/gbSIBhFwQ4s" frameborder="0" allowfullscreen></iframe>Each chromosome consists of one continuous thread-like molecule of DNA coiled tightly around proteins, and contains a portion of the 6,400,000,000 basepairs (DNA building blocks) that make up your DNA. 
Originally created for DNA Interactive ( http://www.dnai.org ).
TRANSCRIPT: In this animation we'll see the remarkable way our DNA is tightly packed up to fit into the nucleus of every cell. The process starts with assembly of a nucleosome, which is formed when eight separate histone protein subunits attach to the DNA molecule. The combined tight loop of DNA and protein is the nucleosome. Six nucleosomes are coiled together and these then stack on top of each other. The end result is a fiber of packed nucleosomes known as chromatin. This structure, is then looped and further packaged using other proteins (which are not shown here) to give the final "chromosomal" shapes. It is this remarkable multiple folding which allows six feet of DNA to fit into the nucleus of each cell in our body. And a typical cell nucleus is so small that ten thousand could fit on the tip of a needle. It is important to realize that chromosomes are not always present, they form only when cells are dividing. At other times, as we can see here at the end of cell division, our DNA becomes less highly organized.)]]></description>
	
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/42965/nucl2vec-local-alignment-of-dna-sequences-using-distributed-vector-representation</guid>
	<pubDate>Tue, 16 Mar 2021 05:45:44 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/42965/nucl2vec-local-alignment-of-dna-sequences-using-distributed-vector-representation</link>
	<title><![CDATA[Nucl2Vec: Local alignment of DNA sequences using Distributed Vector Representation]]></title>
	<description><![CDATA[<p><span>We demonstrate a novel approach for</span><span>local alignment of DNA reads with respect to reference genome.</span><span>For this process we have used Skip-gram model for creating</span><span>encoding(Nucl2Vec) and k-nearest neighbor for the alignment.</span><span>With our new approach we have reduced computation cost for</span><span>local alignment , while achieving accuracy comparable to existing</span><span>defacto standard BWA-MEM tool.</span> </p>
<p><em>https://prakharg24.github.io/papers/401851.full.pdf</em></p><p>Address of the bookmark: <a href="https://prakharg24.github.io/papers/401851.full.pdf" rel="nofollow">https://prakharg24.github.io/papers/401851.full.pdf</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/videolist/watch/20585/dna-transcription-advanced</guid>
	<pubDate>Thu, 29 Jan 2015 05:31:42 -0600</pubDate>
	<link>https://bioinformaticsonline.com/videolist/watch/20585/dna-transcription-advanced</link>
	<title><![CDATA[DNA Transcription (Advanced)]]></title>
	<description><![CDATA[<iframe width="" height="" src="https://www.youtube-nocookie.com/embed/SMtWvDbfHLo" frameborder="0" allowfullscreen></iframe><p>Transcription is the process by which the information in DNA is copied into messenger RNA (mRNA) for protein production. Originally created for DNA Interactive ( http://www.dnai.org ). TRANSCRIPT: The Central Dogma of Molecular Biology: "DNA makes RNA makes protein" Here the process begins. Transcription factors assemble at a specific promoter region along the DNA. The length of DNA following the promoter is a gene and it contains the recipe for a protein. A mediator protein complex arrives carrying the enzyme RNA polymerase. It manoeuvres the RNA polymerase into place... inserting it with the help of other factors between the strands of the DNA double helix. The assembled collection of all these factors is referred to as the transcription initiation complex... and now it is ready to be activated. The initiation complex requires contact with activator proteins, which bind to specific sequences of DNA known as enhancer regions. These regions may be thousands of base pairs distant from the start of the gene. Contact between the activator proteins and the initiation-complex releases the copying mechanism. The RNA polymerase unzips a small portion of the DNA helix exposing the bases on each strand. Only one of the strands is copied. It acts as a template for the synthesis of an RNA molecule which is assembled one sub-unit at a time by matching the DNA letter code on the template strand. The sub-units can be seen here entering the enzyme through its intake hole and they are joined together to form the long messenger RNA chain snaking out of the top.</p>]]></description>
	
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/30831/fsa-fast-statistical-alignment</guid>
	<pubDate>Mon, 06 Feb 2017 04:26:01 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/30831/fsa-fast-statistical-alignment</link>
	<title><![CDATA[FSA: Fast Statistical Alignment]]></title>
	<description><![CDATA[<p><span>FSA is a probabilistic multiple sequence alignment algorithm which uses a "distance-based" approach to aligning homologous protein, RNA or DNA sequences. Much as distance-based phylogenetic reconstruction methods like Neighbor-Joining build a phylogeny using only pairwise divergence estimates, FSA builds a multiple alignment using only pairwise estimations of homology. This is made possible by the sequence annealing technique for constructing a multiple alignment from pairwise comparisons, developed by Ariel Schwartz in&nbsp;</span><a href="http://www.eecs.berkeley.edu/Pubs/TechRpts/2007/EECS-2007-39.html">"Posterior Decoding Methods for Optimization and Control of Multiple Alignments</a><span>."</span></p>
<p>FSA brings the high accuracies previously available only for small-scale analyses of proteins or RNAs to large-scale problems such as aligning thousands of sequences or megabase-long sequences. FSA introduces several novel methods for constructing better alignments:</p>
<ul>
<li>FSA uses machine-learning techniques to estimate gap and substitution parameters on the fly for each set of input sequences. This "query-specific learning" alignment method makes FSA very robust: it can produce superior alignments of sets of homologous sequences which are subject to very different evolutionary constraints.</li>
<li>FSA is capable of aligning hundreds or even thousands of sequences using a randomized inference algorithm to reduce the computational cost of multiple alignment. This randomized inference can be over ten times faster than a direct approach with little loss of accuracy.</li>
<li>FSA can quickly align very long sequences using the "anchor annealing" technique for resolving anchors and projecting them with transitive anchoring. It then stitches together the alignment between the anchors using the methods described above.</li>
<li>The included GUI, MAD (Multiple Alignment Display), can display the intermediate alignments produced by FSA, where each character is colored according to the probability that it is correctly aligned (see the picture and&nbsp;<a href="http://fsa.sourceforge.net/images/Suchard_SIV.fsa.mov">movie</a>&nbsp;at the top of the page).</li>
</ul>
<p><span>You can see more information on the&nbsp;</span><a href="http://fsa.sourceforge.net/FAQ.html">FAQ</a><span>.&nbsp;</span></p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="http://fsa.sourceforge.net/" rel="nofollow">http://fsa.sourceforge.net/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

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