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<channel>
	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/36857?offset=150</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43263/jumbodb-tool-for-de-bruijn-graph-construction</guid>
	<pubDate>Tue, 17 Aug 2021 13:33:46 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43263/jumbodb-tool-for-de-bruijn-graph-construction</link>
	<title><![CDATA[JumboDB: tool for de Bruijn graph construction]]></title>
	<description><![CDATA[<p><span>jumboDB tool for fast de Bruijn graph construction from long sequences (reads or genomes) with very low error rate. JumboDB is not a genome assembler by itself but rather a subroutine that translates a set of reads into compressed de Bruijn graph.</span></p>
<p><span>More at&nbsp;https://github.com/AntonBankevich/jumboDB</span></p><p>Address of the bookmark: <a href="https://github.com/AntonBankevich/jumboDB" rel="nofollow">https://github.com/AntonBankevich/jumboDB</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44483/baclife-an-automated-genome-mining-tool-for-identification-of-lifestyle-associated-genes</guid>
	<pubDate>Fri, 15 Mar 2024 04:59:14 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44483/baclife-an-automated-genome-mining-tool-for-identification-of-lifestyle-associated-genes</link>
	<title><![CDATA[bacLIFE: an automated genome mining tool for identification of lifestyle associated genes]]></title>
	<description><![CDATA[<p style="margin-top: 0px; margin-bottom: 16px; color: #1f2328; font-size: 16px; font-style: normal; font-weight: 400; text-align: start; background-color: #ffffff;" dir="auto">bacLIFE is a streamlined computational workflow that annotates bacterial genomes and performs large-scale comparative genomics to predict bacterial lifestyles and to pinpoint candidate genes, denominated<span>&nbsp;</span><strong style="font-weight: var(--base-text-weight-semibold, 600);">lifestyle-associated genes (LAGs)</strong>, and biosynthetic gene clusters associated with each lifestyle detected. This whole process is divided into different modules:</p>
<ul style="margin-top: 0px; margin-bottom: 16px; color: #1f2328; font-size: 16px; font-style: normal; font-weight: 400; text-align: start; background-color: #ffffff;" dir="auto">
<li><strong style="font-weight: var(--base-text-weight-semibold, 600);">Clustering module</strong><span>&nbsp;</span>Predicts, clusters and annotates the genes of every input genome</li>
<li style="margin-top: 0.25em;"><strong style="font-weight: var(--base-text-weight-semibold, 600);">Lifestyle prediction</strong><span>&nbsp;</span>Employs a machine learning model to forecast bacterial lifestyle or other specified metadata</li>
<li style="margin-top: 0.25em;"><strong style="font-weight: var(--base-text-weight-semibold, 600);">Analitical module (Shiny app)</strong><span>&nbsp;</span>Results from the previous modules are embedded in a user-friendly interface for comprehensive and interactive comparative genomics.</li>
</ul>
<p style="margin-top: 0px; margin-bottom: 16px; color: #1f2328; font-size: 16px; font-style: normal; font-weight: 400; text-align: start; background-color: #ffffff;" dir="auto">You can find the complete wiki here [<a href="https://github.com/Carrion-lab/bacLIFE/wiki/bacLIFE-wiki">https://github.com/Carrion-lab/bacLIFE/wiki/bacLIFE-wiki</a>]</p><p>Address of the bookmark: <a href="https://github.com/Carrion-lab/bacLIFE" rel="nofollow">https://github.com/Carrion-lab/bacLIFE</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44508/a-web-based-tool-for-sequence-alignment-statistics-and-innovative-visualization</guid>
	<pubDate>Thu, 04 Apr 2024 01:44:50 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44508/a-web-based-tool-for-sequence-alignment-statistics-and-innovative-visualization</link>
	<title><![CDATA[A web-based tool for sequence alignment statistics and innovative visualization]]></title>
	<description><![CDATA[<p>AlignStatPlot, a new R package and online tool that is well-documented and easy-to usefor MSA and post-MSA analysis. This tool performs both traditional and cutting-edge analy-ses on sequencing data and generates new visualisation methods for MSA results. Whencompared to currently available tools, AlignStatPlot provides a robust ability to handle andvisualise diversity data, while the online version will save time and encourage researchersto focus on explaining their findings. It is a simple tool that can be used in conjunction withpopulation genetics software (PDF) AlignStatPlot: An R package and online tool for robust sequence alignment statistics and innovative visualization of big data.</p><p>Address of the bookmark: <a href="https://bioinformatics.um6p.ma/AlignStatPlot/" rel="nofollow">https://bioinformatics.um6p.ma/AlignStatPlot/</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44896/jaeger-an-accurate-and-fast-deep-learning-tool-to-detect-bacteriophage-sequences</guid>
	<pubDate>Sun, 31 Aug 2025 06:30:16 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44896/jaeger-an-accurate-and-fast-deep-learning-tool-to-detect-bacteriophage-sequences</link>
	<title><![CDATA[Jaeger : an accurate and fast deep-learning tool to detect bacteriophage sequences]]></title>
	<description><![CDATA[<p><span>Jaeger is a tool that utilizes homology-free machine learning to identify phage genome sequences that are hidden within metagenomes. It is capable of detecting both phages and prophages within metagenomic assemblies.</span></p><p>Address of the bookmark: <a href="https://github.com/MGXlab/Jaeger" rel="nofollow">https://github.com/MGXlab/Jaeger</a></p>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/44801/magic-wormhole-the-easiest-way-to-send-files-securely</guid>
	<pubDate>Wed, 28 May 2025 06:37:17 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/44801/magic-wormhole-the-easiest-way-to-send-files-securely</link>
	<title><![CDATA[Magic Wormhole: The Easiest Way to Send Files Securely]]></title>
	<description><![CDATA[<p>In a world increasingly dependent on digital data exchange, secure and user-friendly file transfer solutions are more important than ever. Enter Magic Wormhole, a deceptively simple yet powerful tool that makes it trivial to send files and messages between computers&mdash;securely and without configuration. Whether you're transferring a PDF to a colleague or sending code snippets between your devices, Magic Wormhole has you covered.</p><p><strong>What is Magic Wormhole?</strong><br />Magic Wormhole is an open-source command-line tool that allows you to securely send files or text from one computer to another. Developed by Brian Warner, it aims to eliminate the usual hassle of file transfers: setting up SSH servers, dealing with firewall rules, cloud storage uploads, or even worrying about man-in-the-middle attacks.</p><p>Using a combination of PAKE (Password-Authenticated Key Exchange) protocols and end-to-end encryption, Magic Wormhole ensures that the only parties who can see your data are you and your recipient.</p><p>&ldquo;It uses PAKE to establish a secure channel between two computers that use the same one-time code.&rdquo;</p><p><strong>How Does It Work?</strong></p><p>One user runs a command like wormhole send file.txt.</p><p>The tool generates a human-readable, one-time code (like 7-horse-staple).</p><p>The other user types wormhole receive and enters the code.</p><p>The file is encrypted, transferred directly (or relayed if needed), and decrypted only on the recipient's side.</p><p>All of this happens over a secure channel, with no manual key exchange, configuration, or trust in a central authority.</p><blockquote><p><strong>Example Usage</strong><br /># Sender<br />wormhole send myfile.pdf<br />Sending 1.4 MB file named 'myfile.pdf'<br />Wormhole code is: 7-horse-staple</p><p># Receiver<br />wormhole receive<br />Please enter code: 7-horse-staple<br />Receiving file (1.4 MB) into: myfile.pdf</p><p><br />That&rsquo;s it! No email attachments, no cloud storage, no FTP setups.</p></blockquote><p>Why Use Magic Wormhole?<br />End-to-end encrypted transfers using modern cryptography.</p><p>Easy to use even for non-technical users.</p><p>Cross-platform: Works on Linux, macOS, and Windows.</p><p>No servers needed (except for a lightweight transit relay).</p><p>Works even behind NAT/firewalls.</p><p><strong>It&rsquo;s particularly ideal for:</strong></p><p>Quickly sharing secrets or passwords.</p><p>Distributing software packages securely.</p><p>Moving files between servers or VMs.</p><p><strong>Under the Hood</strong><br />Magic Wormhole is written in Python and uses:</p><p>SPAKE2 for key exchange.</p><p>Transit relay and Mailbox server for message delivery.</p><p>Twisted framework for asynchronous networking.</p><p>The communication process is decentralized and designed to minimize the trust placed in the relay infrastructure. Even if an attacker intercepts the transit server, they cannot decrypt your data.</p><p><strong>Installation</strong></p><p>You can install it easily with pip:</p><p>pip install magic-wormhole</p><p><br /><strong>There&rsquo;s also a Homebrew package for macOS users</strong>:</p><p>brew install magic-wormhole<br />Community and Ecosystem<br />Magic Wormhole is more than just a file transfer tool. It's part of a growing ecosystem that values user-centric cryptography. There are community-maintained libraries for other languages (e.g., Go, Rust), GUI frontends like wormhole-gui, and integration projects for mobile and web use.</p><p><strong>Limitations</strong></p><p>While Magic Wormhole is elegant and secure, it&rsquo;s primarily a command-line utility and not designed for high-volume or persistent file sharing. Transfers require both sender and receiver to be online at the same time. And since it&rsquo;s peer-to-peer, very large files may suffer performance issues.</p><p><strong>Conclusion</strong><br />Magic Wormhole is a breath of fresh air in the complex world of secure communication. It proves that cryptographic security doesn&rsquo;t need to come with a heavy user experience cost. If you&rsquo;re looking for a simple, secure, and delightful way to send files or messages, give Magic Wormhole a try.</p><p>Explore the documentation: https://magic-wormhole.readthedocs.io</p>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43094/pandoc-a-universal-document-converter</guid>
	<pubDate>Thu, 24 Jun 2021 01:33:47 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43094/pandoc-a-universal-document-converter</link>
	<title><![CDATA[Pandoc: a universal document converter]]></title>
	<description><![CDATA[<p>If you need to convert files from one markup format into another, pandoc is your swiss-army knife. Pandoc can convert almost all formats</p>
<p>https://pandoc.org/index.html</p><p>Address of the bookmark: <a href="https://pandoc.org/" rel="nofollow">https://pandoc.org/</a></p>]]></description>
	<dc:creator>Surabhi Chaudhary</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/37198/understanding-blastn-output-format-6</guid>
	<pubDate>Wed, 27 Jun 2018 18:38:21 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/37198/understanding-blastn-output-format-6</link>
	<title><![CDATA[Understanding BLASTn output format 6 !]]></title>
	<description><![CDATA[<h3 id="sites-page-title-header" style="text-align: left;"><span>BLASTn output format 6</span></h3><div id="sites-canvas-main"><div id="sites-canvas-main-content"><div dir="ltr"><div><div><em>BLASTn</em> maps DNA against DNA, for example gene sequences against a reference genome<br /><br /><code><strong>blastn</strong>  -query <span>genes.ffn</span>  -subject <span>genome.fna</span>  -outfmt <strong>6</strong></code></div><h2>BLASTn tabular output format 6</h2>
<p><strong>Column headers:</strong><br /><code>qseqid sseqid pident length mismatch gapopen qstart qend sstart send evalue bitscore</code><br /></p>
<table border="1" cellspacing="0">
<tbody>
<tr>
<td> 1.</td>
<td> qseqid</td>
<td> query (e.g., gene) sequence id</td>
</tr>
<tr>
<td> 2.</td>
<td> sseqid</td>
<td> subject (e.g., reference genome) sequence id</td>
</tr>
<tr>
<td> 3.</td>
<td> pident</td>
<td> percentage of identical matches</td>
</tr>
<tr>
<td> 4.</td>
<td> length</td>
<td> alignment length</td>
</tr>
<tr>
<td> 5.</td>
<td> mismatch</td>
<td> number of mismatches</td>
</tr>
<tr>
<td> 6.</td>
<td> gapopen</td>
<td> number of gap openings</td>
</tr>
<tr>
<td> 7.</td>
<td> qstart</td>
<td> start of alignment in query</td>
</tr>
<tr>
<td> 8.</td>
<td> qend</td>
<td> end of alignment in query</td>
</tr>
<tr>
<td> 9.</td>
<td> sstart</td>
<td> start of alignment in subject</td>
</tr>
<tr>
<td> 10.</td>
<td> send</td>
<td> end of alignment in subject</td>
</tr>
<tr>
<td> 11.</td>
<td> evalue</td>
<td> <a href="http://www.metagenomics.wiki/tools/blast/evalue">expect value</a></td>
</tr>
<tr>
<td> 12.</td>
<td> bitscore</td>
<td> <a href="http://www.metagenomics.wiki/tools/blast/evalue"><strong>bit score</strong></a></td>
</tr>
</tbody>
</table>
<p><strong><br /></strong></p>
</div><h2><a name="TOC-Define-your-own-output-format" id="TOC-Define-your-own-output-format"></a>Define your own output format</h2><div><em>by adding the option -outfmt, as for example: </em><strong><br /></strong></div>
<p><code><strong>-outfmt</strong> <strong>"6</strong> <span>qseqid sseqid pident qlen length mismatch gapope evalue bitscore</span><strong>"</strong></code><br /><br /><em><strong>supported format specifiers are:</strong></em><br /><code>qseqid    </code>Query Seq-id<br /><code>qgi       </code>Query GI<br /><code>qacc      </code>Query accesion<br /><code>qaccver   </code>Query accesion.version<br /><code>qlen      </code>Query sequence length<br /><code>sseqid    </code>Subject Seq-id<br /><code>sallseqid </code>All subject Seq-id(s), separated by a ';'<br /><code>sgi       </code>Subject GI<br /><code>sallgi    </code>All subject GIs<br /><code>sacc      </code>Subject accession<br /><code>saccver   </code>Subject accession.version<br /><code>sallacc   </code>All subject accessions<br /><code>slen      </code>Subject sequence length<br /><code>qstart    </code>Start of alignment in query<br /><code>qend      </code>End of alignment in query<br /><code>sstart    </code>Start of alignment in subject<br /><code>send      </code>End of alignment in subject<br /><code>qseq      </code>Aligned part of query sequence<br /><code>sseq      </code>Aligned part of subject sequence<br /><code>evalue    </code>Expect value<br /><code>bitscore  </code>Bit score<br /><code>score     </code>Raw score<br /><code>length    </code>Alignment length<br /><code>pident    </code>Percentage of identical matches<br /><code>nident    </code>Number of identical matches<br /><code>mismatch  </code>Number of mismatches<br /><code>positive  </code>Number of positive-scoring matches<br /><code>gapopen   </code>Number of gap openings<br /><code>gaps      </code>Total number of gaps<br /><code>ppos      </code>Percentage of positive-scoring matches<br /><code>frames    </code>Query and subject frames separated by a '/'<br /><code>qframe    </code>Query frame<br /><code>sframe    </code>Subject frame<br /><code>btop      </code>Blast traceback operations (BTOP)<br /><code>staxids   </code>Subject Taxonomy ID(s), separated by a ';'<br /><code>sscinames </code>Subject Scientific Name(s), separated by a ';'<br /><code>scomnames </code>Subject Common Name(s), separated by a ';'<br /><code>sblastnames </code>Subject Blast Name(s), separated by a ';'   (in alphabetical order)<br /><code>sskingdoms  </code>Subject Super Kingdom(s), separated by a ';'     (in alphabetical order) <br /><code>stitle      </code>Subject Title<br /><code>salltitles  </code>All Subject Title(s), separated by a '&lt;&gt;'<br /><code>sstrand   </code>Subject Strand<br /><code>qcovs     </code>Query Coverage Per Subject<br /><code>qcovhsp   </code>Query Coverage Per HSP<br /><strong><br /><em>default values are:</em></strong><br /><code><code>-outfmt "</code>6 qseqid sseqid pident length mismatch gapopen qstart qend sstart send evalue bitscore"</code></p>
</div></div></div>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/file/view/88/regular-expression-cheat-sheet</guid>
	<pubDate>Tue, 09 Jul 2013 17:38:42 -0500</pubDate>
	<link>https://bioinformaticsonline.com/file/view/88/regular-expression-cheat-sheet</link>
	<title><![CDATA[Regular Expression Cheat Sheet]]></title>
	<description><![CDATA[<p><span>The Regular Expression are the sole of Perl language, and for bioinformatician it is just a magical stick to resolve gingatic string data. We did not find any good and user friendly regular expression cheat sheet, hence write our own cheat sheet.&nbsp;</span><span>The Regular Expressions Cheat Sheet, a quick reference guide for regular expressions, including symbols, ranges, grouping, assertions and some sample patterns to get you started.</span></p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
	<enclosure url="https://bioinformaticsonline.com/file/download/88" length="14944" type="application/pdf" />
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/783/perl-module-installation</guid>
	<pubDate>Fri, 12 Jul 2013 11:19:41 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/783/perl-module-installation</link>
	<title><![CDATA[Perl Module Installation]]></title>
	<description><![CDATA[<p>Nice step wide information on perl module installation.</p><p>Address of the bookmark: <a href="http://bioinformaticsonline.com/blog/view/710/how-to-install-perl-modules-manually-using-cpan-command-and-other-quick-ways" rel="nofollow">http://bioinformaticsonline.com/blog/view/710/how-to-install-perl-modules-manually-using-cpan-command-and-other-quick-ways</a></p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/fun/view/2053/perl-poem-parse-it-in-both-perl-and-english</guid>
	<pubDate>Mon, 12 Aug 2013 19:00:55 -0500</pubDate>
	<link>https://bioinformaticsonline.com/fun/view/2053/perl-poem-parse-it-in-both-perl-and-english</link>
	<title><![CDATA[Perl Poem: Parse it in both Perl and English!]]></title>
	<description><![CDATA[<p>Larry's, er, corpus has fortunately been overshadowed by that of the reigning Perl Poet, Sharon Hopkins. She has written quite a few Perl poems, as well as a paper on Perl poetry that she presented at the Usenix Winter 1992 Technical Conference, entitled "Camels and Needles: Computer Poetry Meets the Perl Programming Language". (The paper is available as misc/<a href="http://poetry.ps/" target="_blank">poetry.ps</a>&nbsp;on CPAN.) Besides being the most prolific Perl poet, Sharon is also the most widely published, having had the following poem published in both the Economist and the Guardian:</p><p>#!/usr/bin/perl</p><p>APPEAL:</p><p>listen (please, please);</p><p>open yourself, wide;<br />join (you, me),<br />connect (us,together),</p><p>tell me.</p><p>do something if distressed;</p><p>@dawn, dance;<br />@evening, sing;<br />read (books,$poems,stories) until peaceful;<br />study if able;</p><p>write me if-you-please;</p><p>sort your feelings, reset goals, seek (friends, family, anyone);</p><p>do*not*die (like this)<br />if sin abounds;</p><p>keys (hidden), open (locks, doors), tell secrets;<br />do not, I-beg-you, close them, yet.</p><p>accept (yourself, changes),<br />bind (grief, despair);</p><p>require truth, goodness if-you-will, each moment;</p><p>select (always), length(of-days)</p><p># listen (a perl poem)<br /># Sharon Hopkins<br /># rev. June 19, 1995</p>]]></description>
	<dc:creator>Rahul Agarwal</dc:creator>
</item>

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