BOL: Related items

MashMap: a fast and approximate software for mapping long reads (PacBio/ONT) or assembly to reference genome(s)

Jit — Tue, 12 Dec 2017 17:23:31 -0600

MashMap is a fast and approximate software for mapping long reads (PacBio/ONT) or assembly to reference genome(s). It maps a query sequence against a reference region if and only if its estimated alignment identity is above a specified threshold. It does not compute the alignments explicitly, but rather estimates a k-mer based Jaccard similarity using a combination of Winnowing and MinHash. This is then converted to an estimate of sequence identity using the Mash distance. An appropriate k-mer sampling rate is automatically determined given minimum local alignment length and identity thresholds. The efficiency of the algorithm improves as both of these thresholds are increased.

Address of the bookmark: https://github.com/marbl/MashMap

Bioinformatics Faculty at University of Pune

Fri, 13 Nov 2015 03:06:00 -0600

SAVITRIBAI PHULE PUNE UNIVERSITY
(Formerly University of Pune)
(APPOINTMENT OF TEACHING POSTS)

Accompaniment to the Advertisement No. 52 dated 10.11.2015 for various Teaching Positions purely on contract basis for a maximum period of five years in the various Departments/Centre of the University.

Last date for receipt of application in the University Office is 04.12.2015.

Associate Professor

Environmental Science

Institute of Bioinformatics & Biotechnology (IBB)

Microbiology

Assistant Professor

Biotechnology

Institute of Bioinformatics & Biotechnology (IBB)

Microbiology

More Info : http://collegecirculars.unipune.ac.in/sites/documents/Job%20Openings/advt-no-52-dt-10-11-2015-contract-positions.pdf

Flye: Fast and accurate de novo assembler for single molecule sequencing reads

Jit — Fri, 04 May 2018 19:16:22 -0500

Flye is a de novo assembler for long and noisy reads, such as those produced by PacBio and Oxford Nanopore Technologies. The algorithm uses an A-Bruijn graph to find the overlaps between reads and does not require them to be error-corrected. After the initial assembly, Flye performs an extra repeat classification and analysis step to improve the structural accuracy of the resulting sequence. The package also includes a polisher module, which produces the final assembly of high nucleotide-level quality.

Address of the bookmark: https://github.com/fenderglass/Flye

Postdoctoral positions are available at the Faryabi Lab

Sun, 11 Oct 2015 03:36:18 -0500

Postdoctoral positions are available at the Faryabi Lab located in the Department of Pathology and the Center for Personalized Diagnostics at the University of Pennsylvania.

A qualified candidate will be strongly motivated individual with following qualifications: a Ph.D. in computational sciences, engineering, physics, bioinformatics, or related field. Ability to program in Python/Perl and R in Unix environment, knowledge of C++, Java, and SQL is a plus. Demonstrated applied bioinformatics/computational proficiency as evidenced by relevant publications in peer-reviewed journals. Experience with cancer genomics is a plus.

The primary research focus of the Faryabi Lab is on developing novel computational methods to study mechanisms involved in genome instability and elucidate their roles in tumorigenesis. In addition the position provides collaborative opportunities with both basic and translational scientists across the Perelman School of Medicine, one of the top 5 medical research universities in the nation.
. a Ph.D. degree in either physical or computational sciences
. solid programming skills ( Python/Perl, Java, C/C++, R, SQL)
. computational genomics experience ( high throughput sequence analysis)
. excellent spoken and written communication and interaction skills
. publications in English in relevant areas of research in peer-reviewed journals.
. Previous experience or interest in data visualization design is a plus.

More at
University of Pennsylvania
Pathology and Laboratory Medicine
Robert B. Faryabi
faryabiPenn@gmail.com
http://faryabib.github.io/
How To Apply: email CV and contacts of 3 references to faryabiPenn@gmail.com

BlasR Mapping single molecule sequencing reads using Basic Local Alignment with Successive Refinement (BLASR): Theory and Application,

Jit — Wed, 23 May 2018 06:54:32 -0500

BLASR (Basic Local Alignment with Successive Refinement) for mapping Single Molecule Sequencing (SMS) reads that are thousands to tens of thousands of bases long with divergence between the read and genome dominated by insertion and deletion error.

Here is how I use the blasr to align PacBio reads to the contigs (target.fasta). The “target.fasta.sa” is the suffix array from “target.fasta” generated by sawriter.

blasr query.fa ./target.fasta -sa ./target.fasta.sa -bestn 40 -maxScore -500 -m 4 -nproc 24 -out target.m4 -maxLCPLength 15

the output format option “-m 4″ generate the alignment coordinate. Not fully documented, but I can explain that to you.

I use a 24 cores / 48G ram server for the alignment. It took about 2 to 3 hours aligning 3G PacBio Reads to 10^6 sequences of short read contigs with a mean 3.5kbp length.

Address of the bookmark: http://bix.ucsd.edu/projects/blasr/

Research Associates/Research Fellow - School of Agricultural Biotechnology (Last Date 17-11-2015 and Interview on 26-11-2015 at 11.00 AM)

Fri, 13 Nov 2015 02:46:59 -0600

PAU Recruitment November 2015, Punjab

Job notification in Punjab Agricultural University (PAU) for research Associate/ Research Fellow posts for different projects. Eligible candidates may apply in prescribed application format on or before 17-11-2015 from 09:00 am to 05:00 pm.

Vacancies:
Research Associate/ Research Fellow-03
Qualification:
Candidates should possess B.Sc. with minimum 50% marks, M.Sc. in Bioinformatics/ Biotechnology/ Genetics/ Plant Breeding/ Plant Breeding & Genetics/ Plant Breeding, Genetics & Biotechnology/ Microbiology/ Biochemistry/ Botany with 65% marks.
Selection Process:
Selection will be based on interview.
How to Apply:
Eligible candidates may send their applications along with attested copies of certificates, fee to the Director, School of Agricultural Biotechnology, PAU, Ludhiana on or before 17-11-2015 from 09:00 am to 05:00 pm
Last Date:
17-11-2015

More at http://web.pau.edu/content/banner/1349.pdf

npScarf: real-time scaffolder using SPAdes contigs and Nanopore sequencing reads

Shruti Paniwala — Mon, 11 Jun 2018 05:14:57 -0500

npScarf (jsa.np.npscarf) is a program that connect contigs from a draft genomes to generate sequences that are closer to finish. These pipelines can run on a single laptop for microbial datasets. In real-time mode, it can be integrated with simple structural analyses such as gene ordering, plasmid forming.

Address of the bookmark: http://japsa.readthedocs.io/en/latest/tools/jsa.np.npscarf.html

JRF Bioinformatics

Mon, 02 Nov 2015 03:24:50 -0600

JRF Bioinformatics

Eligibility : ME/M.Tech, MSc(Bio-Chemistry, Bio-Informatics, Bio-Tech, Microbiology), BE/B.Tech

Location : Gandhinagar

Last Date : 05 Nov 2015

Hiring Process : Face to Face Interview

Junior Research Fellow (JRF) Job vacancies in Gujarat State Biotechnology Mission (GSBTM)

Project : “Establishing a network in computational and system biology”

No. of Post : 01

Qualification : MSc. /M.Tech. /B.E. /B.Tech. in Biotechnology/Bioinformatics /Life Sciences /Microbiology /Biochemistry /Genetics from recognized University. Preference would be given to the candidates having NET/GATE.

Fellowship : Rs. 12,000 + 20% HRA
How to apply

Applications should be filled Online. The last date for submission of application is 5th November, 2015.

More at http://btm.gujarat.gov.in/btm/recruitment2.htm

Hercules: a profile HMM-based hybrid error correction algorithm for long reads

Jit — Mon, 20 Aug 2018 14:14:11 -0500

Choosing whether to use second or third generation sequencing platforms can lead to trade-offs between accuracy and read length. Several studies require long and accurate reads including de novo assembly, fusion and structural variation detection. In such cases researchers often combine both technologies and the more erroneous long reads are corrected using the short reads. Current approaches rely on various graph based alignment techniques and do not take the error profile of the underlying technology into account. Memory- and time- efficient machine learning algorithms that address these shortcomings have the potential to achieve better and more accurate integration of these two technologies. Results: We designed and developed Hercules, the first machine learning-based long read error correction algorithm. The algorithm models every long read as a profile Hidden Markov Model with respect to the underlying platformtextquoterights error profile. The algorithm learns a posterior transition/emission probability distribution for each long read and uses this to correct errors in these reads. Using datasets from two DNA-seq BAC clones (CH17-157L1 and CH17-227A2), and human brain cerebellum polyA RNA-seq, we show that Hercules-corrected reads have the highest mapping rate among all competing algorithms and highest accuracy when most of the basepairs of a long read are covered with short reads. Availability:

Hercules source code is available at https://github.com/BilkentCompGen/Hercules

Address of the bookmark: https://github.com/BilkentCompGen/Hercules

Project Assistant-II job vacancies in National Institute of Oceanography (NIO Goa)

Fri, 06 Nov 2015 01:33:22 -0600

Vacancy no : GAP0423/1509-15

Project Assistant-II job vacancies in National Institute of Oceanography (NIO Goa)

Project Title and Code : Biotechnology Information System. -- GAP0423

Qualification : M.Sc Bioinformatics / Zoology / Botany / Environmental Science. Desirable : Basic understanding of biodiversity concept is desirable

No. of Post : 01

Age : 28 Years

Place of Posting : Goa

Consolidated Stipend Per Month : Rs.16000/-

Job Requirement : Collection and handling of biodiversity data as assigned by the scientists and working with in-house biodiversity data management applications and software
How to apply

Candidates satisfying requirements may attend the Walk-in-interview on 12.11.2015, 10.00 AM at NIO Dona Paula, Goa-403004 along with their bio-data, attested copies of certificates & mark lists in proof of qualification (SSC onwards), age, experience and testimonials. Candidates should bring a photo identity proof at the time of registration

More at http://www.nio.org/index/option/com_vacgoa/task/show/title/Goa/tid/6/sid/28/thid/64