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<channel>
	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/36945?offset=240</link>
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	<description><![CDATA[]]></description>
	
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/39821/gvolante-completeness-assessment-of-genometranscriptome-sequences</guid>
	<pubDate>Tue, 06 Aug 2019 21:37:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/39821/gvolante-completeness-assessment-of-genometranscriptome-sequences</link>
	<title><![CDATA[gVolante: Completeness Assessment of Genome/Transcriptome Sequences]]></title>
	<description><![CDATA[<p><strong>gVolante</strong><span>&nbsp;provides an online interface for completeness assessment of user&rsquo;s original or publicly available sequence datasets as well as for browsing results of completeness assessment performed on publicly available genome and transcriptome assemblies.</span></p>
<p><img src="https://gvolante.riken.jp/images/assessment.png" width="937" height="545" alt="image" style="border: 0px;"></p><p>Address of the bookmark: <a href="https://gvolante.riken.jp/" rel="nofollow">https://gvolante.riken.jp/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40613/genome-in-a-bottle-giab-consortium</guid>
	<pubDate>Sat, 25 Jan 2020 13:50:52 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40613/genome-in-a-bottle-giab-consortium</link>
	<title><![CDATA[Genome in a Bottle (GIAB) Consortium]]></title>
	<description><![CDATA[<p><span>The</span><a href="http://www.genomeinabottle.org/"> Genome in a Bottle (GIAB) Consortium</a><span> is a public-private-academic consortium hosted by </span><a href="http://www.nist.gov/" target="_blank">NIST</a><span> to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of whole human genome sequencing to clinical practice. </span></p>
<p><span><a href="https://www.nist.gov/news-events/news/2016/09/nist-releases-new-family-standardized-genomes">https://www.nist.gov/news-events/news/2016/09/nist-releases-new-family-standardized-genomes</a></span></p><p>Address of the bookmark: <a href="https://jimb.stanford.edu/giab/" rel="nofollow">https://jimb.stanford.edu/giab/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/42326/edanchin-lab</guid>
  <pubDate>Thu, 19 Nov 2020 08:00:07 -0600</pubDate>
  <link></link>
  <title><![CDATA[Edanchin Lab]]></title>
  <description><![CDATA[
<p>My main topics of interest are:</p>

<p>The impact of non tree-like evolution such as horizontal gene transfers and hybridization on species biology<br />Evolution and adaptation of animals in the absence of sexual reproduction and the underlying mechanisms<br />Genomic signatures of adaptation to a parasitic life-style</p>

<p>More at https://edanchin.org/</p>
]]></description>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44366/mitofinder</guid>
	<pubDate>Tue, 29 Aug 2023 02:13:01 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44366/mitofinder</link>
	<title><![CDATA[MitoFinder]]></title>
	<description><![CDATA[<p dir="auto">Allio, R., Schomaker-Bastos, A., Romiguier, J., Prosdocimi, F., Nabholz, B., &amp; Delsuc, F. (2020) Mol Ecol Resour. 20, 892-905. (<a href="https://doi.org/10.1111/1755-0998.13160">publication link</a>)</p>
<p dir="auto" style="text-align: center;"><a href="https://github.com/RemiAllio/MitoFinder/blob/master/image/logo.png" target="_blank"><img src="https://github.com/RemiAllio/MitoFinder/raw/master/image/logo.png" alt="Drawing" width="250" style="border: 0px;"></a></p>
<p dir="auto"><span>Mitofinder</span>&nbsp;is a pipeline to&nbsp;<span>assemble</span>&nbsp;mitochondrial genomes and&nbsp;<span>annotate</span>&nbsp;mitochondrial genes from trimmed read sequencing data.</p>
<p dir="auto"><span>MitoFinder</span>&nbsp;is also designed to&nbsp;<span>find</span>&nbsp;and&nbsp;<span>annotate</span>&nbsp;mitochondrial sequences in existing genomic assemblies (generated from Hifi/PacBio/Nanopore/Illumina sequencing data...)</p>
<p dir="auto"><span>MitoFinder</span>&nbsp;is distributed under the&nbsp;<a href="https://github.com/RemiAllio/MitoFinder/blob/master/License/LICENSE">license</a>.</p><p>Address of the bookmark: <a href="https://github.com/RemiAllio/MitoFinder" rel="nofollow">https://github.com/RemiAllio/MitoFinder</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43427/ogdraw-draw-organelle-genome-maps</guid>
	<pubDate>Tue, 05 Oct 2021 03:34:35 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43427/ogdraw-draw-organelle-genome-maps</link>
	<title><![CDATA[OGDRAW - Draw Organelle Genome Maps]]></title>
	<description><![CDATA[<p>OrganellarGenomeDRAW converts annotations in the&nbsp;<a href="https://www.ncbi.nlm.nih.gov/genbank/">GenBank</a>&nbsp;or&nbsp;<a href="https://www.ebi.ac.uk/ena">EMBL/ENA</a>&nbsp;format into graphical maps. The input has to be a&nbsp;<a href="https://www.ncbi.nlm.nih.gov/Sitemap/samplerecord.html">GenBank&nbsp;</a>or&nbsp;<a href="https://www.ebi.ac.uk/ena/submit/flat-file">EMBL/ENA flat file</a>&nbsp;wherase the output can vary among several types of files. The application is optimized to create detailed high-quality maps of organellar genomes (plastid and mitochondria). Nevertheless, you can upload most<span style="color: #0b0118;">&nbsp;database</span>&nbsp;entries.</p>
<p>&nbsp;</p>
<p>Please take a look at our&nbsp;<a href="https://chlorobox.mpimp-golm.mpg.de/OGDraw-FAQ.html">FAQ section</a>&nbsp;and do not hesitate to report bugs or suggestions for improvements by&nbsp;<a href="mailto:chlorobox@mpimp-golm.mpg.de?subject=OGDRAW">email</a>.</p><p>Address of the bookmark: <a href="https://chlorobox.mpimp-golm.mpg.de/OGDraw.html" rel="nofollow">https://chlorobox.mpimp-golm.mpg.de/OGDraw.html</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/43670/useful-bioinformatics-analysis-tools</guid>
	<pubDate>Thu, 23 Dec 2021 23:10:02 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/43670/useful-bioinformatics-analysis-tools</link>
	<title><![CDATA[Useful Bioinformatics Analysis Tools !]]></title>
	<description><![CDATA[<h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=cometa&amp;subpage=about">CoMeta</a></h3><p><strong>Classificier of reads from metagenomic sequencing experiments.</strong></p><p><span>&bull;&nbsp;&nbsp;Kawulok, J., Deorowicz, S.,&nbsp;</span><em>CoMeta: Classification of Metagenomes Using k-mers</em><span>,&nbsp;</span><a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121453">PLOS ONE,&nbsp;</a><span>2015; 10(4):1&ndash;23,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=CoMSA&amp;subpage=about">CoMSA</a></h3><p><strong>Compressor of multiple sequence alignments of proteins.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Walczyszyn, J., Debudaj-Grabysz, A.,&nbsp;</span><em>CoMSA: compression of protein multiple sequence alignment files</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty619">Bioinformatics,&nbsp;</a><span>2019; 35(2):22&ndash;234,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=dsrc&amp;subpage=about">DSRC</a></h3><p><strong>Compressor of sequencing reads.</strong></p><p><span>&bull;&nbsp;&nbsp;Roguski, L., Deorowicz, S.,&nbsp;</span><em>DSRC 2: Industry-oriented compression of FASTQ files</em><span>,&nbsp;</span><a href="http://bioinformatics.oxfordjournals.org/content/30/15/2213">Bioinformatics,&nbsp;</a><span>2014; 30(15):2213&ndash;2215,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Grabowski, Sz.,&nbsp;</span><em>Compression of DNA sequences in FASTQ format</em><span>,&nbsp;</span><a href="http://bioinformatics.oxfordjournals.org/">Bioinformatics,&nbsp;</a><span>2011; 27(6):860&ndash;862,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=famsa&amp;subpage=about">FAMSA</a></h3><p><strong>Multiple sequence alignment designed for huge families of proteins (even containing hundreds of thousands of sequences).</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Debudaj-Grabysz, A., Gudys, A.,&nbsp;</span><em>FAMSA: Fast and accurate multiple sequence alignment of huge protein families</em><span>,&nbsp;</span><a href="http://www.nature.com/articles/srep33964">Scientific Reports,&nbsp;</a><span>2016; 6(33964):</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=fastore&amp;subpage=about">FaStore</a></h3><p><strong>Compressor of FASTQ files.</strong></p><p><span>&bull;&nbsp;&nbsp;Roguski, L., Ochoa, I., Hernaez, M., Deorowicz, S.,&nbsp;</span><em>FaStore - a space-saving solution for raw sequencing data</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty205">Bioinformatics,&nbsp;</a><span>2018; 34(16):2748&ndash;2756,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=fqsqueezer&amp;subpage=about">FQSqueezer</a></h3><p><strong>Experimental high-end compressor of FASTQ files.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S.,&nbsp;</span><em>FQSqueezer: k-mer-based compression of sequencing data</em><span>,&nbsp;</span><a href="https://www.nature.com/articles/s41598-020-57452-6">Scientific Reports,&nbsp;</a><span>2020; 10(578):</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=gdc&amp;subpage=about">GDC</a></h3><p><strong>Compressor of collections of genome sequences.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Danek, A., Niemiec, M.,&nbsp;</span><em>GDC 2: Compression of large collections of genomes</em><span>,&nbsp;</span><a href="http://www.nature.com/srep/2015/150625/srep11565/full/srep11565.html">Scientific Reports,&nbsp;</a><span>2015; 5(11565):1&ndash;12,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Grabowski, Sz.,&nbsp;</span><em>Robust relative compression of genomes with random access</em><span>,&nbsp;</span><a href="http://sun.aei.polsl.pl/REFRESH/bioinformatics.oxfordjournals.org/content/27/21/2979.abstract">Bioinformatics,&nbsp;</a><span>2011; 27(21):2979&ndash;2986,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=gtc&amp;subpage=about">GTC</a></h3><p><strong>Genotype databases compressor with support for fast queries.</strong></p><p><span>&bull;&nbsp;&nbsp;Danek, A., Deorowicz, S.,&nbsp;</span><em>GTC: how to maintain huge genotype collections in a compressed form</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty023">Bioinformatics,&nbsp;</a><span>2018; 34(11):1834&ndash;1840,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=gtshark&amp;subpage=about">GTShark</a></h3><p><strong>Genotypes compressor.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Danek, A.,&nbsp;</span><em>GTShark: Genotype compression in large projects</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/btz508">Bioinformatics,&nbsp;</a><span>2019; 35(22):4791&ndash;4793,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=kmc&amp;subpage=about">KMC</a></h3><p><strong>Memory frugal&nbsp;<em>k</em>-mer counter.</strong></p><p><span>&bull;&nbsp;&nbsp;Kokot, M., Długosz, M., Deorowicz, S.,&nbsp;</span><em>KMC 3: counting and manipulating k -mer statistics</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/btx304">Bioinformatics,&nbsp;</a><span>2017; 33(17):2759&ndash;2761,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Kokot, M., Grabowski, Sz., Debudaj-Grabysz, A.,&nbsp;</span><em>KMC 2: Fast and resource-frugal k-mer counting</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/btv022">Bioinformatics,&nbsp;</a><span>2015; 31(10):1569&ndash;1576,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Debudaj-Grabysz, A., Grabowski, Sz.,&nbsp;</span><em>Disk-based k-mer counting on a PC</em><span>,&nbsp;</span><a href="http://www.biomedcentral.com/1471-2105/14/160">BMC Bioinformatics,&nbsp;</a><span>2013; 14():Article no. 160,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=kmer-db&amp;subpage=about">Kmer-db</a></h3><p><strong>Tool for estimation of evolutionary distances in a collection of genomes.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Gudys, A., Dlugosz, M., Kokot, M., Danek, A.,&nbsp;</span><em>Kmer-db: instant evolutionary distance estimation</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty610">Bioinformatics,&nbsp;</a><span>2019; 35(1):133&ndash;136,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=mugi&amp;subpage=about">MuGI</a></h3><p><strong>Index allowing queries for a collection of multiple genome sequences.</strong></p><p><span>&bull;&nbsp;&nbsp;Danek, A., Deorowicz, S., Grabowski, Sz.,&nbsp;</span><em>Indexes of Large Genome Collections on a PC</em><span>,&nbsp;</span><a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0109384">PLOS ONE,&nbsp;</a><span>2014; 9(10):e109384,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=orcom&amp;subpage=about">ORCOM</a></h3><p><strong>Experimental compressor of sequencing reads.</strong></p><p><span>&bull;&nbsp;&nbsp;Grabowski, Sz., Deorowicz, S., Roguski, L.,&nbsp;</span><em>Disk-based compression of data from genome sequencing</em><span>,&nbsp;</span><a href="http://bioinformatics.oxfordjournals.org/content/early/2014/12/22/bioinformatics.btu844.abstract">Bioinformatics,&nbsp;</a><span>2014; 31(9):1389&ndash;1395,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=pgsa&amp;subpage=about">PgSA</a></h3><p><strong>Index allowing queries for a collection of sequencing reads.</strong></p><p><span>&bull;&nbsp;&nbsp;Kowalski, T., Grabowski, Sz., Deorowicz, S.,&nbsp;</span><em>Indexing arbitrary-length k-mers in sequencing reads</em><span>,&nbsp;</span><a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133198">PLOS ONE,&nbsp;</a><span>2015; 10(7):1&ndash;16,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=quickprobs&amp;subpage=about">QuickProbs</a></h3><p><strong>Multiple sequence alignment designed especially for GPU.</strong></p><p><span>&bull;&nbsp;&nbsp;Gudys, A., Deorowicz, S.,&nbsp;</span><em>QuickProbs 2: towards rapid construction of high-quality alignments of large protein families</em><span>,&nbsp;</span><a href="http://www.nature.com/articles/srep41553">Scientific Reports,&nbsp;</a><span>2017; 7(41553):</span><br /><span>&bull;&nbsp;&nbsp;Gudys, A., Deorowicz, S.,&nbsp;</span><em>QuickProbs &ndash; A Fast Multiple Sequence Alignment Algorithm Designed for Graphics Processors</em><span>,&nbsp;</span><a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0088901">PLOS ONE,&nbsp;</a><span>2014; 9(2):e88901,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=reckoner&amp;subpage=about">RECKONER</a></h3><p><strong>Read error corrector.</strong></p><p><span>&bull;&nbsp;&nbsp;Maciej Długosz, M., Deorowicz, S.,&nbsp;</span><em>RECKONER: read error corrector based on KMC</em><span>,&nbsp;</span><a href="https://academic.oup.com/bioinformatics/article-abstract/33/7/1086/2843893/RECKONER-read-error-corrector-based-on-KMC">Bioinformatics,&nbsp;</a><span>2017; 33(7):1086&ndash;1089,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=tgc&amp;subpage=about">TGC</a></h3><p><strong>Compressor of collections of genomes given in Variant Call Format (VCF) files.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Danek, A., Grabowski, Sz.,&nbsp;</span><em>Genome compression: a novel approach for large collections</em><span>,&nbsp;</span><a href="http://bioinformatics.oxfordjournals.org/content/early/2013/08/29/bioinformatics.btt460">Bioinformatics,&nbsp;</a><span>2013; 29(20):2572&ndash;2578,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=vcfshark&amp;subpage=about">VCFShark</a></h3><p><strong>Compressor of VCF files.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Danek, A.,&nbsp;</span><em>GTShark: Genotype compression in large projects</em><span>,&nbsp;</span><a href="https://www.biorxiv.org/content/10.1101/2020.12.18.423437v1">biorxiv.org,&nbsp;</a><span>2020; ():</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=whisper&amp;subpage=about">Whisper</a></h3><p><strong>Experimental mapper of whole genome sequencing data.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Gudys, A.,&nbsp;</span><em>Whisper 2: indel-sensitive short read mapping</em><span>,&nbsp;</span><a href="https://doi.org/10.1101/2019.12.18.881292">bioRxiv.org,&nbsp;</a><span>2019; :</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Debudaj-Grabysz, A., Gudys, A., Grabowski, Sz.,&nbsp;</span><em>Whisper: read sorting allows robust robust mapping of DNA sequencing data</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty927">Bioinformatics,&nbsp;</a><span>2019; 35(12):2043&ndash;2050,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Debudaj-Grabysz, A., Gudys, A., Grabowski, Sz.,&nbsp;</span><em>Robust mapping of whole genome sequencing data</em><span>,&nbsp;</span><a href="https://meetings.cshl.edu/abstracts.aspx?meet=GENOME&amp;year=17">Poster at The Biology of Genomes Conference,&nbsp;</a><span>2017;</span></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43799/kast</guid>
	<pubDate>Wed, 23 Feb 2022 08:28:36 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43799/kast</link>
	<title><![CDATA[KAST]]></title>
	<description><![CDATA[<p><span>Perform Alignment-free k-tuple frequency comparisons from sequences. This can be in the form of two input files (e.g. a reference and a query) or a single file for pairwise comparisons to be made.</span></p><p>Address of the bookmark: <a href="https://github.com/martinjvickers/KAST" rel="nofollow">https://github.com/martinjvickers/KAST</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44223/ale-assembly-likelihood-estimator</guid>
	<pubDate>Wed, 08 Mar 2023 01:39:33 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44223/ale-assembly-likelihood-estimator</link>
	<title><![CDATA[ALE: Assembly Likelihood Estimator]]></title>
	<description><![CDATA[<p>Just import the assembly, bam and ALE scores. You can convert the .ale file to a set of .wig files with ale2wiggle.py and IGV can read those directly.&nbsp; Depending on your genome size you may want to convert the .wig files to the BigWig format.</p><p>Address of the bookmark: <a href="https://github.com/sc932/ALE" rel="nofollow">https://github.com/sc932/ALE</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/44371/steps-to-find-all-the-repeats-in-the-genome</guid>
	<pubDate>Thu, 31 Aug 2023 02:43:28 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/44371/steps-to-find-all-the-repeats-in-the-genome</link>
	<title><![CDATA[Steps to find all the repeats in the genome !]]></title>
	<description><![CDATA[<div><p>To find repeats in a genome from 2 to 9 length using a Perl script, you can use the RepeatMasker tool with the "--length" option<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>. Here's a step-by-step guide:</p></div><div><ol>
<li>Install RepeatMasker: First, you need to install RepeatMasker on your system. You can download it from the RepeatMasker website<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>.</li>
</ol></div><div><ol>
<li>Prepare the genome sequence: Make sure you have the genome sequence in a FASTA file format. Let's assume the file is named "genome.fasta".</li>
</ol><blockquote><p>./RepeatMasker -pa &lt;number_of_processors&gt; -nolow -norna -no_is -div &lt;divergence_value&gt; -lib RepeatMaskerLib.embl -gff -xsmall -small -poly -species &lt;species_name&gt; -dir &lt;output_directory&gt; -length &lt;min_length&gt;-&lt;max_length&gt; genome.fasta</p></blockquote><div><p>Replace the following placeholders with appropriate values:</p><ul>
<li><code>&lt;number_of_processors&gt;</code>: The number of processors/threads you want to use for parallel processing.</li>
<li><code>&lt;divergence_value&gt;</code>: The divergence value for the species you are analyzing. You can find divergence values for different species in the RepeatMasker documentation<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>.</li>
<li><code>&lt;species_name&gt;</code>: The name of the species you are analyzing.</li>
<li><code>&lt;output_directory&gt;</code>: The directory where you want the output files to be saved.</li>
<li><code>&lt;min_length&gt;</code>&nbsp;and&nbsp;<code>&lt;max_length&gt;</code>: The minimum and maximum lengths of the repeats you want to find (in this case, 2 and 9).</li>
</ul></div><div><ol>
<li>Analyze the output: RepeatMasker will generate several output files, including a .out file. You can parse this file to extract the information you need. There is a Perl tool called "one_code_to_find_them_all.pl" that can help you parse RepeatMasker output files<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>. You can download it from the source provided.</li>
</ol></div><div><ol>
<li>Use the provided Perl script: Once you have the "one_code_to_find_them_all.pl" script, you can run it to conveniently parse the RepeatMasker output files. Here's an example of how to use it:</li>
</ol><blockquote><p>perl one_code_to_find_them_all.pl --rm &lt;RepeatMasker_out_file&gt; --length &lt;length_file&gt;</p></blockquote></div><p>&nbsp;</p></div><div><div><p>Replace&nbsp;<code>&lt;RepeatMasker_out_file&gt;</code>&nbsp;with the path to your RepeatMasker .out file, and&nbsp;<code>&lt;length_file&gt;</code>&nbsp;with the path to a file containing the lengths of the reference elements.</p></div><div><p>This script will generate several output files, including .log.txt and .copynumber.csv, which contain quantitative information about the identified repeat elements.</p></div><div><p>Remember to adjust the parameters and options according to your specific needs and the characteristics of your genome.</p></div></div>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/44637/tools-to-access-the-quality-of-your-assembled-genome</guid>
	<pubDate>Thu, 08 Aug 2024 23:31:18 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/44637/tools-to-access-the-quality-of-your-assembled-genome</link>
	<title><![CDATA[Tools to access the quality of your assembled genome !]]></title>
	<description><![CDATA[<ul dir="auto">
<li><a href="https://github.com/linsalrob/fasta_validator">FASTA VALIDATOR</a>&nbsp;+&nbsp;<a href="https://github.com/shenwei356/seqkit">SEQKIT RMDUP</a>: FASTA validation</li>
<li><a href="https://genometools.org/tools/gt_gff3validator.html">GENOMETOOLS GT GFF3VALIDATOR</a>: GFF3 validation</li>
<li><a href="https://github.com/PlantandFoodResearch/assemblathon2-analysis/blob/a93cba25d847434f7eadc04e63b58c567c46a56d/assemblathon_stats.pl">ASSEMBLATHON STATS</a>: Assembly statistics</li>
<li><a href="https://genometools.org/tools/gt_stat.html">GENOMETOOLS GT STAT</a>: Annotation statistics</li>
<li><a href="https://github.com/ncbi/fcs">NCBI FCS ADAPTOR</a>: Adaptor contamination pass/fail</li>
<li><a href="https://github.com/ncbi/fcs">NCBI FCS GX</a>: Foreign organism contamination pass/fail</li>
<li><a href="https://gitlab.com/ezlab/busco">BUSCO</a>: Gene-space completeness estimation</li>
<li><a href="https://github.com/tolkit/telomeric-identifier">TIDK</a>: Telomere repeat identification</li>
<li><a href="https://github.com/oushujun/LTR_retriever/blob/master/LAI">LAI</a>: Continuity of repetitive sequences</li>
<li><a href="https://github.com/DerrickWood/kraken2">KRAKEN2</a>: Taxonomy classification</li>
<li><a href="https://github.com/igvteam/juicebox.js">HIC CONTACT MAP</a>: Alignment and visualisation of HiC data</li>
<li><a href="https://github.com/mummer4/mummer">MUMMER</a>&nbsp;&rarr;&nbsp;<a href="http://circos.ca/documentation/">CIRCOS</a>&nbsp;+&nbsp;<a href="https://plotly.com/">DOTPLOT</a>&nbsp;&amp;&nbsp;<a href="https://github.com/lh3/minimap2">MINIMAP2</a>&nbsp;&rarr;&nbsp;<a href="https://github.com/schneebergerlab/plotsr">PLOTSR</a>: Synteny analysis</li>
<li><a href="https://github.com/marbl/merqury">MERQURY</a>: K-mer completeness, consensus quality and phasing assessment</li>
</ul>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>

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