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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/36945?offset=270</link>
	<atom:link href="https://bioinformaticsonline.com/related/36945?offset=270" rel="self" type="application/rss+xml" />
	<description><![CDATA[]]></description>
	
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38801/genome-assembly-forensics-finding-the-elusive-mis-assembly</guid>
	<pubDate>Sat, 26 Jan 2019 18:02:01 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38801/genome-assembly-forensics-finding-the-elusive-mis-assembly</link>
	<title><![CDATA[Genome assembly forensics: finding the elusive mis-assembly]]></title>
	<description><![CDATA[<p><span>We present the first collection of tools aimed at automated genome assembly validation. This work formalizes several mechanisms for detecting mis-assemblies, and describes their implementation in our automated validation pipeline, called&nbsp;</span><em>amosvalidate</em><span>. We demonstrate the application of our pipeline in both bacterial and eukaryotic genome assemblies, and highlight several assembly errors in both draft and finished genomes. The software described is compatible with common assembly formats and is released, open-source, at&nbsp;</span><a href="http://amos.sourceforge.net/" target="_blank">http://amos.sourceforge.net</a><span>.</span></p>
<p>https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2397507/&nbsp;</p>
<p>http://amos.sourceforge.net/wiki/index.php/AMOS</p><p>Address of the bookmark: <a href="http://amos.sourceforge.net/wiki/index.php/AMOS" rel="nofollow">http://amos.sourceforge.net/wiki/index.php/AMOS</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/39821/gvolante-completeness-assessment-of-genometranscriptome-sequences</guid>
	<pubDate>Tue, 06 Aug 2019 21:37:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/39821/gvolante-completeness-assessment-of-genometranscriptome-sequences</link>
	<title><![CDATA[gVolante: Completeness Assessment of Genome/Transcriptome Sequences]]></title>
	<description><![CDATA[<p><strong>gVolante</strong><span>&nbsp;provides an online interface for completeness assessment of user&rsquo;s original or publicly available sequence datasets as well as for browsing results of completeness assessment performed on publicly available genome and transcriptome assemblies.</span></p>
<p><img src="https://gvolante.riken.jp/images/assessment.png" width="937" height="545" alt="image" style="border: 0px;"></p><p>Address of the bookmark: <a href="https://gvolante.riken.jp/" rel="nofollow">https://gvolante.riken.jp/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40613/genome-in-a-bottle-giab-consortium</guid>
	<pubDate>Sat, 25 Jan 2020 13:50:52 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40613/genome-in-a-bottle-giab-consortium</link>
	<title><![CDATA[Genome in a Bottle (GIAB) Consortium]]></title>
	<description><![CDATA[<p><span>The</span><a href="http://www.genomeinabottle.org/"> Genome in a Bottle (GIAB) Consortium</a><span> is a public-private-academic consortium hosted by </span><a href="http://www.nist.gov/" target="_blank">NIST</a><span> to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of whole human genome sequencing to clinical practice. </span></p>
<p><span><a href="https://www.nist.gov/news-events/news/2016/09/nist-releases-new-family-standardized-genomes">https://www.nist.gov/news-events/news/2016/09/nist-releases-new-family-standardized-genomes</a></span></p><p>Address of the bookmark: <a href="https://jimb.stanford.edu/giab/" rel="nofollow">https://jimb.stanford.edu/giab/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/42326/edanchin-lab</guid>
  <pubDate>Thu, 19 Nov 2020 08:00:07 -0600</pubDate>
  <link></link>
  <title><![CDATA[Edanchin Lab]]></title>
  <description><![CDATA[
<p>My main topics of interest are:</p>

<p>The impact of non tree-like evolution such as horizontal gene transfers and hybridization on species biology<br />Evolution and adaptation of animals in the absence of sexual reproduction and the underlying mechanisms<br />Genomic signatures of adaptation to a parasitic life-style</p>

<p>More at https://edanchin.org/</p>
]]></description>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44366/mitofinder</guid>
	<pubDate>Tue, 29 Aug 2023 02:13:01 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44366/mitofinder</link>
	<title><![CDATA[MitoFinder]]></title>
	<description><![CDATA[<p dir="auto">Allio, R., Schomaker-Bastos, A., Romiguier, J., Prosdocimi, F., Nabholz, B., &amp; Delsuc, F. (2020) Mol Ecol Resour. 20, 892-905. (<a href="https://doi.org/10.1111/1755-0998.13160">publication link</a>)</p>
<p dir="auto" style="text-align: center;"><a href="https://github.com/RemiAllio/MitoFinder/blob/master/image/logo.png" target="_blank"><img src="https://github.com/RemiAllio/MitoFinder/raw/master/image/logo.png" alt="Drawing" width="250" style="border: 0px;"></a></p>
<p dir="auto"><span>Mitofinder</span>&nbsp;is a pipeline to&nbsp;<span>assemble</span>&nbsp;mitochondrial genomes and&nbsp;<span>annotate</span>&nbsp;mitochondrial genes from trimmed read sequencing data.</p>
<p dir="auto"><span>MitoFinder</span>&nbsp;is also designed to&nbsp;<span>find</span>&nbsp;and&nbsp;<span>annotate</span>&nbsp;mitochondrial sequences in existing genomic assemblies (generated from Hifi/PacBio/Nanopore/Illumina sequencing data...)</p>
<p dir="auto"><span>MitoFinder</span>&nbsp;is distributed under the&nbsp;<a href="https://github.com/RemiAllio/MitoFinder/blob/master/License/LICENSE">license</a>.</p><p>Address of the bookmark: <a href="https://github.com/RemiAllio/MitoFinder" rel="nofollow">https://github.com/RemiAllio/MitoFinder</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43427/ogdraw-draw-organelle-genome-maps</guid>
	<pubDate>Tue, 05 Oct 2021 03:34:35 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43427/ogdraw-draw-organelle-genome-maps</link>
	<title><![CDATA[OGDRAW - Draw Organelle Genome Maps]]></title>
	<description><![CDATA[<p>OrganellarGenomeDRAW converts annotations in the&nbsp;<a href="https://www.ncbi.nlm.nih.gov/genbank/">GenBank</a>&nbsp;or&nbsp;<a href="https://www.ebi.ac.uk/ena">EMBL/ENA</a>&nbsp;format into graphical maps. The input has to be a&nbsp;<a href="https://www.ncbi.nlm.nih.gov/Sitemap/samplerecord.html">GenBank&nbsp;</a>or&nbsp;<a href="https://www.ebi.ac.uk/ena/submit/flat-file">EMBL/ENA flat file</a>&nbsp;wherase the output can vary among several types of files. The application is optimized to create detailed high-quality maps of organellar genomes (plastid and mitochondria). Nevertheless, you can upload most<span style="color: #0b0118;">&nbsp;database</span>&nbsp;entries.</p>
<p>&nbsp;</p>
<p>Please take a look at our&nbsp;<a href="https://chlorobox.mpimp-golm.mpg.de/OGDraw-FAQ.html">FAQ section</a>&nbsp;and do not hesitate to report bugs or suggestions for improvements by&nbsp;<a href="mailto:chlorobox@mpimp-golm.mpg.de?subject=OGDRAW">email</a>.</p><p>Address of the bookmark: <a href="https://chlorobox.mpimp-golm.mpg.de/OGDraw.html" rel="nofollow">https://chlorobox.mpimp-golm.mpg.de/OGDraw.html</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/43670/useful-bioinformatics-analysis-tools</guid>
	<pubDate>Thu, 23 Dec 2021 23:10:02 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/43670/useful-bioinformatics-analysis-tools</link>
	<title><![CDATA[Useful Bioinformatics Analysis Tools !]]></title>
	<description><![CDATA[<h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=cometa&amp;subpage=about">CoMeta</a></h3><p><strong>Classificier of reads from metagenomic sequencing experiments.</strong></p><p><span>&bull;&nbsp;&nbsp;Kawulok, J., Deorowicz, S.,&nbsp;</span><em>CoMeta: Classification of Metagenomes Using k-mers</em><span>,&nbsp;</span><a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121453">PLOS ONE,&nbsp;</a><span>2015; 10(4):1&ndash;23,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=CoMSA&amp;subpage=about">CoMSA</a></h3><p><strong>Compressor of multiple sequence alignments of proteins.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Walczyszyn, J., Debudaj-Grabysz, A.,&nbsp;</span><em>CoMSA: compression of protein multiple sequence alignment files</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty619">Bioinformatics,&nbsp;</a><span>2019; 35(2):22&ndash;234,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=dsrc&amp;subpage=about">DSRC</a></h3><p><strong>Compressor of sequencing reads.</strong></p><p><span>&bull;&nbsp;&nbsp;Roguski, L., Deorowicz, S.,&nbsp;</span><em>DSRC 2: Industry-oriented compression of FASTQ files</em><span>,&nbsp;</span><a href="http://bioinformatics.oxfordjournals.org/content/30/15/2213">Bioinformatics,&nbsp;</a><span>2014; 30(15):2213&ndash;2215,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Grabowski, Sz.,&nbsp;</span><em>Compression of DNA sequences in FASTQ format</em><span>,&nbsp;</span><a href="http://bioinformatics.oxfordjournals.org/">Bioinformatics,&nbsp;</a><span>2011; 27(6):860&ndash;862,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=famsa&amp;subpage=about">FAMSA</a></h3><p><strong>Multiple sequence alignment designed for huge families of proteins (even containing hundreds of thousands of sequences).</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Debudaj-Grabysz, A., Gudys, A.,&nbsp;</span><em>FAMSA: Fast and accurate multiple sequence alignment of huge protein families</em><span>,&nbsp;</span><a href="http://www.nature.com/articles/srep33964">Scientific Reports,&nbsp;</a><span>2016; 6(33964):</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=fastore&amp;subpage=about">FaStore</a></h3><p><strong>Compressor of FASTQ files.</strong></p><p><span>&bull;&nbsp;&nbsp;Roguski, L., Ochoa, I., Hernaez, M., Deorowicz, S.,&nbsp;</span><em>FaStore - a space-saving solution for raw sequencing data</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty205">Bioinformatics,&nbsp;</a><span>2018; 34(16):2748&ndash;2756,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=fqsqueezer&amp;subpage=about">FQSqueezer</a></h3><p><strong>Experimental high-end compressor of FASTQ files.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S.,&nbsp;</span><em>FQSqueezer: k-mer-based compression of sequencing data</em><span>,&nbsp;</span><a href="https://www.nature.com/articles/s41598-020-57452-6">Scientific Reports,&nbsp;</a><span>2020; 10(578):</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=gdc&amp;subpage=about">GDC</a></h3><p><strong>Compressor of collections of genome sequences.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Danek, A., Niemiec, M.,&nbsp;</span><em>GDC 2: Compression of large collections of genomes</em><span>,&nbsp;</span><a href="http://www.nature.com/srep/2015/150625/srep11565/full/srep11565.html">Scientific Reports,&nbsp;</a><span>2015; 5(11565):1&ndash;12,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Grabowski, Sz.,&nbsp;</span><em>Robust relative compression of genomes with random access</em><span>,&nbsp;</span><a href="http://sun.aei.polsl.pl/REFRESH/bioinformatics.oxfordjournals.org/content/27/21/2979.abstract">Bioinformatics,&nbsp;</a><span>2011; 27(21):2979&ndash;2986,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=gtc&amp;subpage=about">GTC</a></h3><p><strong>Genotype databases compressor with support for fast queries.</strong></p><p><span>&bull;&nbsp;&nbsp;Danek, A., Deorowicz, S.,&nbsp;</span><em>GTC: how to maintain huge genotype collections in a compressed form</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty023">Bioinformatics,&nbsp;</a><span>2018; 34(11):1834&ndash;1840,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=gtshark&amp;subpage=about">GTShark</a></h3><p><strong>Genotypes compressor.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Danek, A.,&nbsp;</span><em>GTShark: Genotype compression in large projects</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/btz508">Bioinformatics,&nbsp;</a><span>2019; 35(22):4791&ndash;4793,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=kmc&amp;subpage=about">KMC</a></h3><p><strong>Memory frugal&nbsp;<em>k</em>-mer counter.</strong></p><p><span>&bull;&nbsp;&nbsp;Kokot, M., Długosz, M., Deorowicz, S.,&nbsp;</span><em>KMC 3: counting and manipulating k -mer statistics</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/btx304">Bioinformatics,&nbsp;</a><span>2017; 33(17):2759&ndash;2761,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Kokot, M., Grabowski, Sz., Debudaj-Grabysz, A.,&nbsp;</span><em>KMC 2: Fast and resource-frugal k-mer counting</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/btv022">Bioinformatics,&nbsp;</a><span>2015; 31(10):1569&ndash;1576,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Debudaj-Grabysz, A., Grabowski, Sz.,&nbsp;</span><em>Disk-based k-mer counting on a PC</em><span>,&nbsp;</span><a href="http://www.biomedcentral.com/1471-2105/14/160">BMC Bioinformatics,&nbsp;</a><span>2013; 14():Article no. 160,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=kmer-db&amp;subpage=about">Kmer-db</a></h3><p><strong>Tool for estimation of evolutionary distances in a collection of genomes.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Gudys, A., Dlugosz, M., Kokot, M., Danek, A.,&nbsp;</span><em>Kmer-db: instant evolutionary distance estimation</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty610">Bioinformatics,&nbsp;</a><span>2019; 35(1):133&ndash;136,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=mugi&amp;subpage=about">MuGI</a></h3><p><strong>Index allowing queries for a collection of multiple genome sequences.</strong></p><p><span>&bull;&nbsp;&nbsp;Danek, A., Deorowicz, S., Grabowski, Sz.,&nbsp;</span><em>Indexes of Large Genome Collections on a PC</em><span>,&nbsp;</span><a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0109384">PLOS ONE,&nbsp;</a><span>2014; 9(10):e109384,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=orcom&amp;subpage=about">ORCOM</a></h3><p><strong>Experimental compressor of sequencing reads.</strong></p><p><span>&bull;&nbsp;&nbsp;Grabowski, Sz., Deorowicz, S., Roguski, L.,&nbsp;</span><em>Disk-based compression of data from genome sequencing</em><span>,&nbsp;</span><a href="http://bioinformatics.oxfordjournals.org/content/early/2014/12/22/bioinformatics.btu844.abstract">Bioinformatics,&nbsp;</a><span>2014; 31(9):1389&ndash;1395,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=pgsa&amp;subpage=about">PgSA</a></h3><p><strong>Index allowing queries for a collection of sequencing reads.</strong></p><p><span>&bull;&nbsp;&nbsp;Kowalski, T., Grabowski, Sz., Deorowicz, S.,&nbsp;</span><em>Indexing arbitrary-length k-mers in sequencing reads</em><span>,&nbsp;</span><a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133198">PLOS ONE,&nbsp;</a><span>2015; 10(7):1&ndash;16,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=quickprobs&amp;subpage=about">QuickProbs</a></h3><p><strong>Multiple sequence alignment designed especially for GPU.</strong></p><p><span>&bull;&nbsp;&nbsp;Gudys, A., Deorowicz, S.,&nbsp;</span><em>QuickProbs 2: towards rapid construction of high-quality alignments of large protein families</em><span>,&nbsp;</span><a href="http://www.nature.com/articles/srep41553">Scientific Reports,&nbsp;</a><span>2017; 7(41553):</span><br /><span>&bull;&nbsp;&nbsp;Gudys, A., Deorowicz, S.,&nbsp;</span><em>QuickProbs &ndash; A Fast Multiple Sequence Alignment Algorithm Designed for Graphics Processors</em><span>,&nbsp;</span><a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0088901">PLOS ONE,&nbsp;</a><span>2014; 9(2):e88901,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=reckoner&amp;subpage=about">RECKONER</a></h3><p><strong>Read error corrector.</strong></p><p><span>&bull;&nbsp;&nbsp;Maciej Długosz, M., Deorowicz, S.,&nbsp;</span><em>RECKONER: read error corrector based on KMC</em><span>,&nbsp;</span><a href="https://academic.oup.com/bioinformatics/article-abstract/33/7/1086/2843893/RECKONER-read-error-corrector-based-on-KMC">Bioinformatics,&nbsp;</a><span>2017; 33(7):1086&ndash;1089,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=tgc&amp;subpage=about">TGC</a></h3><p><strong>Compressor of collections of genomes given in Variant Call Format (VCF) files.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Danek, A., Grabowski, Sz.,&nbsp;</span><em>Genome compression: a novel approach for large collections</em><span>,&nbsp;</span><a href="http://bioinformatics.oxfordjournals.org/content/early/2013/08/29/bioinformatics.btt460">Bioinformatics,&nbsp;</a><span>2013; 29(20):2572&ndash;2578,</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=vcfshark&amp;subpage=about">VCFShark</a></h3><p><strong>Compressor of VCF files.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Danek, A.,&nbsp;</span><em>GTShark: Genotype compression in large projects</em><span>,&nbsp;</span><a href="https://www.biorxiv.org/content/10.1101/2020.12.18.423437v1">biorxiv.org,&nbsp;</a><span>2020; ():</span></p><h3><a href="http://sun.aei.polsl.pl/REFRESH/index.php?page=projects&amp;project=whisper&amp;subpage=about">Whisper</a></h3><p><strong>Experimental mapper of whole genome sequencing data.</strong></p><p><span>&bull;&nbsp;&nbsp;Deorowicz, S., Gudys, A.,&nbsp;</span><em>Whisper 2: indel-sensitive short read mapping</em><span>,&nbsp;</span><a href="https://doi.org/10.1101/2019.12.18.881292">bioRxiv.org,&nbsp;</a><span>2019; :</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Debudaj-Grabysz, A., Gudys, A., Grabowski, Sz.,&nbsp;</span><em>Whisper: read sorting allows robust robust mapping of DNA sequencing data</em><span>,&nbsp;</span><a href="https://doi.org/10.1093/bioinformatics/bty927">Bioinformatics,&nbsp;</a><span>2019; 35(12):2043&ndash;2050,</span><br /><span>&bull;&nbsp;&nbsp;Deorowicz, S., Debudaj-Grabysz, A., Gudys, A., Grabowski, Sz.,&nbsp;</span><em>Robust mapping of whole genome sequencing data</em><span>,&nbsp;</span><a href="https://meetings.cshl.edu/abstracts.aspx?meet=GENOME&amp;year=17">Poster at The Biology of Genomes Conference,&nbsp;</a><span>2017;</span></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43799/kast</guid>
	<pubDate>Wed, 23 Feb 2022 08:28:36 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43799/kast</link>
	<title><![CDATA[KAST]]></title>
	<description><![CDATA[<p><span>Perform Alignment-free k-tuple frequency comparisons from sequences. This can be in the form of two input files (e.g. a reference and a query) or a single file for pairwise comparisons to be made.</span></p><p>Address of the bookmark: <a href="https://github.com/martinjvickers/KAST" rel="nofollow">https://github.com/martinjvickers/KAST</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44223/ale-assembly-likelihood-estimator</guid>
	<pubDate>Wed, 08 Mar 2023 01:39:33 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44223/ale-assembly-likelihood-estimator</link>
	<title><![CDATA[ALE: Assembly Likelihood Estimator]]></title>
	<description><![CDATA[<p>Just import the assembly, bam and ALE scores. You can convert the .ale file to a set of .wig files with ale2wiggle.py and IGV can read those directly.&nbsp; Depending on your genome size you may want to convert the .wig files to the BigWig format.</p><p>Address of the bookmark: <a href="https://github.com/sc932/ALE" rel="nofollow">https://github.com/sc932/ALE</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/44371/steps-to-find-all-the-repeats-in-the-genome</guid>
	<pubDate>Thu, 31 Aug 2023 02:43:28 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/44371/steps-to-find-all-the-repeats-in-the-genome</link>
	<title><![CDATA[Steps to find all the repeats in the genome !]]></title>
	<description><![CDATA[<div><p>To find repeats in a genome from 2 to 9 length using a Perl script, you can use the RepeatMasker tool with the "--length" option<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>. Here's a step-by-step guide:</p></div><div><ol>
<li>Install RepeatMasker: First, you need to install RepeatMasker on your system. You can download it from the RepeatMasker website<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>.</li>
</ol></div><div><ol>
<li>Prepare the genome sequence: Make sure you have the genome sequence in a FASTA file format. Let's assume the file is named "genome.fasta".</li>
</ol><blockquote><p>./RepeatMasker -pa &lt;number_of_processors&gt; -nolow -norna -no_is -div &lt;divergence_value&gt; -lib RepeatMaskerLib.embl -gff -xsmall -small -poly -species &lt;species_name&gt; -dir &lt;output_directory&gt; -length &lt;min_length&gt;-&lt;max_length&gt; genome.fasta</p></blockquote><div><p>Replace the following placeholders with appropriate values:</p><ul>
<li><code>&lt;number_of_processors&gt;</code>: The number of processors/threads you want to use for parallel processing.</li>
<li><code>&lt;divergence_value&gt;</code>: The divergence value for the species you are analyzing. You can find divergence values for different species in the RepeatMasker documentation<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>.</li>
<li><code>&lt;species_name&gt;</code>: The name of the species you are analyzing.</li>
<li><code>&lt;output_directory&gt;</code>: The directory where you want the output files to be saved.</li>
<li><code>&lt;min_length&gt;</code>&nbsp;and&nbsp;<code>&lt;max_length&gt;</code>: The minimum and maximum lengths of the repeats you want to find (in this case, 2 and 9).</li>
</ul></div><div><ol>
<li>Analyze the output: RepeatMasker will generate several output files, including a .out file. You can parse this file to extract the information you need. There is a Perl tool called "one_code_to_find_them_all.pl" that can help you parse RepeatMasker output files<a href="https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-5-13" target="_blank">[0]</a>. You can download it from the source provided.</li>
</ol></div><div><ol>
<li>Use the provided Perl script: Once you have the "one_code_to_find_them_all.pl" script, you can run it to conveniently parse the RepeatMasker output files. Here's an example of how to use it:</li>
</ol><blockquote><p>perl one_code_to_find_them_all.pl --rm &lt;RepeatMasker_out_file&gt; --length &lt;length_file&gt;</p></blockquote></div><p>&nbsp;</p></div><div><div><p>Replace&nbsp;<code>&lt;RepeatMasker_out_file&gt;</code>&nbsp;with the path to your RepeatMasker .out file, and&nbsp;<code>&lt;length_file&gt;</code>&nbsp;with the path to a file containing the lengths of the reference elements.</p></div><div><p>This script will generate several output files, including .log.txt and .copynumber.csv, which contain quantitative information about the identified repeat elements.</p></div><div><p>Remember to adjust the parameters and options according to your specific needs and the characteristics of your genome.</p></div></div>]]></description>
	<dc:creator>Neel</dc:creator>
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