Rahul Nayak
A Bioinformatician
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Common steps for reads mapping !
By BioStar 625 days agoMapping reads to a reference genome is an essential step in many types of genomic analysis, such as variant calling and gene expression analysis.
My commonly used commands in Bioinformatics
Last updated 2312 days ago by Rahul NayakFYI, I've found it useful to use MUMmer to extract the specific changes that Racon makes, so I can evaluate them individually: minimap -t 24 assembly.fasta long_reads.fastq.gz | racon -t 24 long_reads.fastq.gz - assembly.fasta...NanoPack: visualizing and processing long-read sequencing data
By Jit 2160 days agogithub.com - The NanoPack tools are written in Python3 and released under the GNU GPL3.0 License. The source code can be found at https://github.com/wdecoster/nanopack, together with links to separate scripts and their documentation. The scripts are compatible...Kevler: Reference-free variant discovery in large eukaryotic genomes
By Jit 1761 days agogithub.com - Welcome to kevlar, software for predicting de novo genetic variants without mapping reads to a reference genome! kevlar's k-mer abundance based method calls single nucleotide variants (SNVs), multinucleotide variants (MNVs),...RNA-Seq Data Pathway and Gene-set Analysis Workflows
By Jit 4047 days agoDescribe the GAGE workflows on RNA-Seq data pathway analysis and gene-set analysis.BioJupies: Automatically Generates RNA-seq Data Analysis Notebooks
By Rahul Nayak 1434 days agoamp.pharm.mssm.edu - With BioJupies you can produce in seconds a customized, reusable, and interactive report from your own raw or processed RNA-seq data through a simple user interface BioJupies now supports user accounts! Sign in from the top right corner of the page...RNA-seq workflow: gene-level exploratory analysis and differential expression
By Jit 1141 days agomaster.bioconductor.org - Here we walk through an end-to-end gene-level RNA-seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were quantified to the reference transcripts, and prepare gene-level count...-
Pathway Analysis
Last updated 3698 days ago by Rahul Agarwal Comments (2)Pathway Analysis is usually performed with aim to enrich the genes with their functional information and reveal the underlying biological mechanisms pursue by genes. Pathway Analysis is not only limited to what biological pathways a particular set... Webinar on Unique Molecular Identifier (UMI)-powered Ultra-sensitive Variant Calling using Strand...
By Strand 2573 days ago Comments (1)Webinar on Unique Molecular Identifier-powered Ultra-sensitive Variant Calling using Strand NGS - Case Study by Dr. Pandurang Kolekar, Bioinformatics Engineer, Strand Life Sciences Abstract: Unique Molecular Identifiers (UMIs) are short...GeneSpring webinar- Uncovering mechanisms of hepatotoxicity on 14 June at 8AM PST
By Yeshodari 2741 days agoUncovering Mechanisms of Hepatotoxicity for High Affinity Antisense Oligonucleotides – 3’ end RNA-seq Profiling Using GeneSpring GX High affinity antisense oligonucleotides (ASOs) containing bicylic modifications (BNA) such as locked...
