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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/37840?offset=580</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/21443/a-guide-for-complete-r-beginners-getting-data-into-r</guid>
	<pubDate>Tue, 24 Feb 2015 20:15:08 -0600</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/21443/a-guide-for-complete-r-beginners-getting-data-into-r</link>
	<title><![CDATA[A guide for complete R beginners :- Getting data into R]]></title>
	<description><![CDATA[<p>For a beginner this can be is the hardest part, it is also the most important to get right.</p><p>It is possible to create a vector by typing data directly into R using the combine function &lsquo;c&rsquo;</p><blockquote><p><strong>x </strong></p></blockquote><p>same as</p><blockquote><p><strong>x </strong></p></blockquote><p>creates the vector x with the numbers between 1 and 5.</p><p>You can see what is in an object at any time by typing its name;</p><blockquote><p><strong>x</strong></p></blockquote><p>will produce the output<strong> &lsquo;[1] 1 2 3 4 5&prime;</strong></p><p>Note that names need to be quoted</p><blockquote><p><strong>daysofweek </strong><strong>&larr; c(&lsquo;Monday&rsquo;, &lsquo;Tuesday&rsquo;, &lsquo;Wednesday&rsquo;, &lsquo;Thursday&rsquo;, &lsquo;Friday&rsquo;);</strong></p></blockquote><p>Usually however you want to input from a file. We have touched on the &lsquo;read.table&rsquo; function already.</p><blockquote><p><strong>mydata </strong></p></blockquote><p>Now <strong>mydata</strong> is a data frame with multiple vectors</p><p>each vector can be identified by the default syntax</p><p>#if any of these are typed it will print to screen</p><blockquote><p><strong>mydata$V1 mydata$V2 mydata$V3 </strong></p></blockquote><p>By default the function assumes certain things from the file</p><ul>
<li>The file is a plain text file (there are function to read excel files: <em>not covered here</em>)</li>
<li>columns are separated by any number of tabs or spaces</li>
<li>there is the same number of data points in each column</li>
<li>there is no header row (labels for the columns)</li>
<li>there is no column with names for the rows** [I&rsquo;ll explain].</li>
</ul><p><span style="text-decoration: underline;">If any of these are false, we need to tell that to the function</span></p><p>If it has a header column</p><blockquote><p><strong>mydata <em>header=T also works</em></strong></p></blockquote><p>Note that there is a comma between different parts of the functions arguments</p><p>If there is one less column in the header row, then R assumes that the 1<sup>st</sup> column of data after the header are the row names</p><p>Now the vectors (columns) are identified by their name</p><p>#if any of these are typed it will print to screen</p><blockquote><p><strong>mydata$A mydata$B mydata$C </strong></p></blockquote><p># Summary about the whole data frame</p><blockquote><p><strong>summary(mydata)</strong></p></blockquote><p># Summary information of column A</p><blockquote><p><strong>summary(mydata$A) </strong></p></blockquote><p>We can shortcut having to type the data frame each time by attaching it</p><blockquote><p><strong>attach(mydata)</strong></p></blockquote><p># summary of column B as &lsquo;mydata&rsquo; is attached</p><blockquote><p><strong>summary(B)</strong></p></blockquote><p><span style="text-decoration: underline;">Two other important options for </span><em><span style="text-decoration: underline;">read.table</span></em></p><p>If is is separated only by tabs and has a header</p><blockquote><p><strong>mydata </strong></p></blockquote><p>Really useful if you have spaces in the contents of some columns, so R does not mess up reading the columns . However if the columns or of an uneven length it will tell you.</p><p>If you know that the file has uneven columns</p><blockquote><p><strong>mydata </strong></p></blockquote><p>This causes R to fill empty spaces in a columns with &lsquo;NA&rsquo; .</p><p>The last two examples will still work with our file and give the same result as with only headers=T</p><p><span style="text-decoration: underline;">Graphs</span></p><p>to get an idea of what R is capable of type</p><blockquote><p><strong>demo(graphics)</strong></p></blockquote><p>steps through the examples, and the code is printed to the screen</p><p>We will work with simpler examples that have immediate use to biologists.</p><p>Remember to get more information about the options to a function type &lsquo;?function&rsquo;</p><p><span style="text-decoration: underline;">Histogram of A</span><span style="text-decoration: underline;"></span></p><blockquote><p><strong>hist(mydata$A)</strong></p></blockquote><p>If there was more data we could increase the number of vertical columns with the option, breaks=50 (or another relevant number).</p><blockquote><p><strong>boxplot(mydata)</strong></p></blockquote><p>We can get rid of the need to type the data frame each time by using the <strong>attach</strong> function</p><p># if not already done so</p><blockquote><p><strong>attach(mydata) </strong></p><p><strong>boxplot(mydata$A, mydata$B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p>same as</p><blockquote><p><strong>boxplot(A, B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p><span style="text-decoration: underline;">Scatter plot</span></p><p># if not already done so</p><blockquote><p><strong>attach(mydata) </strong></p><p><strong>plot(A,B) # or plot(mydata$A, mydata$B)</strong></p></blockquote><p><strong><span style="text-decoration: underline;">SAVING an image</span></strong></p><p>Windows users (Rgui) RIGHT click on image and select which you want.</p><p><span style="text-decoration: underline;">These instructions work for everyone.</span></p><p>You need to create a new device of the type of file you need, then send the data to that device</p><p>to save as a png file (easy to load into the likes of powerpoint, also great for web applications.</p><blockquote><p><strong>png(&lsquo;filename&rsquo;) </strong></p><p><strong>boxplot(A, B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p>or to save as a pdf</p><blockquote><p><strong>pdf(&lsquo;filename&rsquo;) </strong></p><p><strong>boxplot(A, B, name=c(&ldquo;Value A&rdquo;, &ldquo;Value B&rdquo;) , ylab=&ldquo;Count of Something&rdquo;)</strong></p></blockquote><p><span style="text-decoration: underline;">Note</span></p><ul>
<li>Nothing will appear on screen, the output is going to the file</li>
<li>Also it may not be saved immediately but will once the device (or R) is turned quit.</li>
</ul><p>To quit R type</p><p><strong>q() # </strong>If you save your session, next time you start R, you will have your data preloaded.</p><p>Or if you want to remain in R</p><blockquote><pre><strong>dev.off() #</strong>turns of the png (or pdf etc) device, thus forces the data to save</pre></blockquote>]]></description>
	<dc:creator>Archana Malhotra</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/23160/opencpu</guid>
	<pubDate>Sun, 05 Jul 2015 18:34:46 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/23160/opencpu</link>
	<title><![CDATA[OpenCPU]]></title>
	<description><![CDATA[<p>OpenCPU is a system for embedded scientific computing and reproducible research. The OpenCPU server provides a reliable and interoperable <a href="https://www.opencpu.org/api.html">HTTP API</a> for data analysis based on R.</p><p>The OpenCPU <a href="https://www.opencpu.org/jslib.html">JavaScript client library</a> provides the most seamless integration of R and JavaScript available today.</p><p>OpenCPU uses standard R packaging to develop, ship and deploy web applications. Several open source <a href="https://www.opencpu.org/apps.html">example apps</a> are available from Github.</p><p>Installing your own OpenCPU server is <a href="https://www.opencpu.org/download.html">super easy</a> and only takes a few minutes.</p><p>More at https://www.opencpu.org/</p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27225/painless-package-development-for-r</guid>
	<pubDate>Tue, 03 May 2016 05:31:06 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27225/painless-package-development-for-r</link>
	<title><![CDATA[Painless package development for R]]></title>
	<description><![CDATA[<p>Devtools makes package development a breeze: it works with R&rsquo;s existing conventions for code structure, adding efficient tools to support the cycle of package development. With devtools, developing a package becomes so easy that it will be your default layout whenever you&rsquo;re writing a significant amount of code.</p>
<p>Before you get started be sure to check out:</p>
<ul>
<li><a href="https://groups.google.com/forum/#%21forum/rdevtools" title="Google devtools Group">devtools Google Group &ndash;&nbsp;https://groups.google.com/forum/#!forum/rdevtools</a></li>
<li><a href="http://adv-r.had.co.nz/" title="Hadley W Online Book">book on &ldquo;Advanced R programming&rdquo; &ndash;&nbsp;http://adv-r.had.co.nz/</a></li>
<li><a href="https://github.com/hadley/devtools" title="devtools GitHub">GitHub repository &ndash;&nbsp;https://github.com/hadley/devtools</a></li>
</ul>
<h3 id="getting_started">&nbsp;</h3><p>Address of the bookmark: <a href="https://www.rstudio.com/products/rpackages/devtools/" rel="nofollow">https://www.rstudio.com/products/rpackages/devtools/</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/33398/tiny-python36-notebook</guid>
	<pubDate>Sat, 03 Jun 2017 03:16:28 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/33398/tiny-python36-notebook</link>
	<title><![CDATA[Tiny Python3.6 Notebook]]></title>
	<description><![CDATA[<p><span>This is not so much an instructional manual, but rather notes, tables, and examples for Python syntax. It was created by the author as an additional resource during training, meant to be distributed as a physical notebook. Participants (who favor the physical characteristics of dead tree material) could add their own notes, thoughts, and have a valuable reference of curated examples.</span></p><p>Address of the bookmark: <a href="https://github.com/mattharrison/Tiny-Python-3.6-Notebook/blob/master/python.rst" rel="nofollow">https://github.com/mattharrison/Tiny-Python-3.6-Notebook/blob/master/python.rst</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43022/a-simple-tutorial-for-a-complex-complexheatmap</guid>
	<pubDate>Fri, 02 Apr 2021 06:18:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43022/a-simple-tutorial-for-a-complex-complexheatmap</link>
	<title><![CDATA[A simple tutorial for a complex ComplexHeatmap]]></title>
	<description><![CDATA[<p><em>ComplexHeatmap</em>&nbsp;(Gu, Eils, and Schlesner (2016)) is an R Programming Language (R Core Team (2020)) package that is currently listed in the&nbsp;<a href="https://bioconductor.org/">Bioconductor</a>&nbsp;package repository.</p>
<p><a href="https://github.com/kevinblighe/E-MTAB-6141#2-install-and-load-required-packages">install and load required packages</a></p>
<div>
<pre>  require(<span>RColorBrewer</span>)
  require(<span>ComplexHeatmap</span>)
  require(<span>circlize</span>)
  require(<span>digest</span>)
  require(<span>cluster</span>)</pre>
</div>
<p>If all load successfully, proceed to&nbsp;<span>Part 3</span>. Otherwise, go through the following code chunks in order to ensure that each package is installed and loaded properly.</p>
<p><em>BiocManager</em>&nbsp;(Morgan (2019))</p><p>Address of the bookmark: <a href="https://github.com/kevinblighe/E-MTAB-6141" rel="nofollow">https://github.com/kevinblighe/E-MTAB-6141</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/39307/awk-for-beginners</guid>
	<pubDate>Fri, 26 Apr 2019 16:19:41 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/39307/awk-for-beginners</link>
	<title><![CDATA[AWK for beginners !]]></title>
	<description><![CDATA[<p>AWK is a standard tool on every POSIX-compliant UNIX system. It&rsquo;s like flex/lex, from the command-line, perfect for text-processing tasks and other scripting needs. It has a C-like syntax, but without mandatory semicolons (although, you should use them anyway, because they are required when you&rsquo;re writing one-liners, something AWK excels at), manual memory management, or static typing. It excels at text processing. You can call to it from a shell script, or you can use it as a stand-alone scripting language.</p><p>Why use AWK instead of Perl? Readability. AWK is easier to read than Perl. For simple text-processing scripts, particularly ones that read files line by line and split on delimiters, AWK is probably the right tool for the job.</p><div><pre><span>#!/usr/bin/awk -f</span>

<span># Comments are like this</span>


<span># AWK programs consist of a collection of patterns and actions.</span>
<span>pattern1</span> <span>{</span> <span>action</span><span>;</span> <span>}</span> <span># just like lex</span>
<span>pattern2</span> <span>{</span> <span>action</span><span>;</span> <span>}</span>

<span># There is an implied loop and AWK automatically reads and parses each</span>
<span># record of each file supplied. Each record is split by the FS delimiter,</span>
<span># which defaults to white-space (multiple spaces,tabs count as one)</span>
<span># You can assign FS either on the command line (-F C) or in your BEGIN</span>
<span># pattern</span>

<span># One of the special patterns is BEGIN. The BEGIN pattern is true</span>
<span># BEFORE any of the files are read. The END pattern is true after</span>
<span># an End-of-file from the last file (or standard-in if no files specified)</span>
<span># There is also an output field separator (OFS) that you can assign, which</span>
<span># defaults to a single space</span>

<span>BEGIN</span> <span>{</span>

    <span># BEGIN will run at the beginning of the program. It's where you put all</span>
    <span># the preliminary set-up code, before you process any text files. If you</span>
    <span># have no text files, then think of BEGIN as the main entry point.</span>

    <span># Variables are global. Just set them or use them, no need to declare..</span>
    <span>count</span> <span>=</span> <span>0</span><span>;</span>

    <span># Operators just like in C and friends</span>
    <span>a</span> <span>=</span> <span>count</span> <span>+</span> <span>1</span><span>;</span>
    <span>b</span> <span>=</span> <span>count</span> <span>-</span> <span>1</span><span>;</span>
    <span>c</span> <span>=</span> <span>count</span> <span>*</span> <span>1</span><span>;</span>
    <span>d</span> <span>=</span> <span>count</span> <span>/</span> <span>1</span><span>;</span> <span># integer division</span>
    <span>e</span> <span>=</span> <span>count</span> <span>%</span> <span>1</span><span>;</span> <span># modulus</span>
    <span>f</span> <span>=</span> <span>count</span> <span>^</span> <span>1</span><span>;</span> <span># exponentiation</span>

    <span>a</span> <span>+=</span> <span>1</span><span>;</span>
    <span>b</span> <span>-=</span> <span>1</span><span>;</span>
    <span>c</span> <span>*=</span> <span>1</span><span>;</span>
    <span>d</span> <span>/=</span> <span>1</span><span>;</span>
    <span>e</span> <span>%=</span> <span>1</span><span>;</span>
    <span>f</span> <span>^=</span> <span>1</span><span>;</span>

    <span># Incrementing and decrementing by one</span>
    <span>a</span><span>++</span><span>;</span>
    <span>b</span><span>--</span><span>;</span>

    <span># As a prefix operator, it returns the incremented value</span>
    <span>++</span><span>a</span><span>;</span>
    <span>--</span><span>b</span><span>;</span>

    <span># Notice, also, no punctuation such as semicolons to terminate statements</span>

    <span># Control statements</span>
    <span>if</span> <span>(</span><span>count</span> <span>==</span> <span>0</span><span>)</span>
        <span>print</span> <span>"Starting with count of 0"</span><span>;</span>
    <span>else</span>
        <span>print</span> <span>"Huh?"</span><span>;</span>

    <span># Or you could use the ternary operator</span>
    <span>print</span> <span>(</span><span>count</span> <span>==</span> <span>0</span><span>)</span> <span>?</span> <span>"Starting with count of 0"</span> <span>:</span> <span>"Huh?"</span><span>;</span>

    <span># Blocks consisting of multiple lines use braces</span>
    <span>while</span> <span>(</span><span>a</span> <span>&lt;</span> <span>10</span><span>)</span> <span>{</span>
        <span>print</span> <span>"String concatenation is done"</span> <span>" with a series"</span> <span>" of"</span>
            <span>" space-separated strings"</span><span>;</span>
        <span>print</span> <span>a</span><span>;</span>

        <span>a</span><span>++</span><span>;</span>
    <span>}</span>

    <span>for</span> <span>(</span><span>i</span> <span>=</span> <span>0</span><span>;</span> <span>i</span> <span>&lt;</span> <span>10</span><span>;</span> <span>i</span><span>++</span><span>)</span>
        <span>print</span> <span>"Good ol' for loop"</span><span>;</span>

    <span># As for comparisons, they're the standards:</span>
    <span># a &lt; b   # Less than</span>
    <span># a &lt;= b  # Less than or equal</span>
    <span># a != b  # Not equal</span>
    <span># a == b  # Equal</span>
    <span># a &gt; b   # Greater than</span>
    <span># a &gt;= b  # Greater than or equal</span>

    <span># Logical operators as well</span>
    <span># a &amp;&amp; b  # AND</span>
    <span># a || b  # OR</span>

    <span># In addition, there's the super useful regular expression match</span>
    <span>if</span> <span>(</span><span>"foo"</span> <span>~</span> <span>"^fo+$"</span><span>)</span>
        <span>print</span> <span>"Fooey!"</span><span>;</span>
    <span>if</span> <span>(</span><span>"boo"</span> <span>!~</span> <span>"^fo+$"</span><span>)</span>
        <span>print</span> <span>"Boo!"</span><span>;</span>

    <span># Arrays</span>
    <span>arr</span><span>[</span><span>0</span><span>]</span> <span>=</span> <span>"foo"</span><span>;</span>
    <span>arr</span><span>[</span><span>1</span><span>]</span> <span>=</span> <span>"bar"</span><span>;</span>

    <span># You can also initialize an array with the built-in function split()</span>

    <span>n</span> <span>=</span> <span>split</span><span>(</span><span>"foo:bar:baz"</span><span>,</span> <span>arr</span><span>,</span> <span>":"</span><span>);</span>

    <span># You also have associative arrays (actually, they're all associative arrays)</span>
    <span>assoc</span><span>[</span><span>"foo"</span><span>]</span> <span>=</span> <span>"bar"</span><span>;</span>
    <span>assoc</span><span>[</span><span>"bar"</span><span>]</span> <span>=</span> <span>"baz"</span><span>;</span>

    <span># And multi-dimensional arrays, with some limitations I won't mention here</span>
    <span>multidim</span><span>[</span><span>0</span><span>,</span><span>0</span><span>]</span> <span>=</span> <span>"foo"</span><span>;</span>
    <span>multidim</span><span>[</span><span>0</span><span>,</span><span>1</span><span>]</span> <span>=</span> <span>"bar"</span><span>;</span>
    <span>multidim</span><span>[</span><span>1</span><span>,</span><span>0</span><span>]</span> <span>=</span> <span>"baz"</span><span>;</span>
    <span>multidim</span><span>[</span><span>1</span><span>,</span><span>1</span><span>]</span> <span>=</span> <span>"boo"</span><span>;</span>

    <span># You can test for array membership</span>
    <span>if</span> <span>(</span><span>"foo"</span> <span>in</span> <span>assoc</span><span>)</span>
        <span>print</span> <span>"Fooey!"</span><span>;</span>

    <span># You can also use the 'in' operator to traverse the keys of an array</span>
    <span>for</span> <span>(</span><span>key</span> <span>in</span> <span>assoc</span><span>)</span>
        <span>print</span> <span>assoc</span><span>[</span><span>key</span><span>];</span>

    <span># The command line is in a special array called ARGV</span>
    <span>for</span> <span>(</span><span>argnum</span> <span>in</span> <span>ARGV</span><span>)</span>
        <span>print</span> <span>ARGV</span><span>[</span><span>argnum</span><span>];</span>

    <span># You can remove elements of an array</span>
    <span># This is particularly useful to prevent AWK from assuming the arguments</span>
    <span># are files for it to process</span>
    <span>delete</span> <span>ARGV</span><span>[</span><span>1</span><span>];</span>

    <span># The number of command line arguments is in a variable called ARGC</span>
    <span>print</span> <span>ARGC</span><span>;</span>

    <span># AWK has several built-in functions. They fall into three categories. I'll</span>
    <span># demonstrate each of them in their own functions, defined later.</span>

    <span>return_value</span> <span>=</span> <span>arithmetic_functions</span><span>(</span><span>a</span><span>,</span> <span>b</span><span>,</span> <span>c</span><span>);</span>
    <span>string_functions</span><span>();</span>
    <span>io_functions</span><span>();</span>
<span>}</span>

<span># Here's how you define a function</span>
<span>function</span> <span>arithmetic_functions</span><span>(</span><span>a</span><span>,</span> <span>b</span><span>,</span> <span>c</span><span>,</span>     <span>d</span><span>)</span> <span>{</span>

    <span># Probably the most annoying part of AWK is that there are no local</span>
    <span># variables. Everything is global. For short scripts, this is fine, even</span>
    <span># useful, but for longer scripts, this can be a problem.</span>

    <span># There is a work-around (ahem, hack). Function arguments are local to the</span>
    <span># function, and AWK allows you to define more function arguments than it</span>
    <span># needs. So just stick local variable in the function declaration, like I</span>
    <span># did above. As a convention, stick in some extra whitespace to distinguish</span>
    <span># between actual function parameters and local variables. In this example,</span>
    <span># a, b, and c are actual parameters, while d is merely a local variable.</span>

    <span># Now, to demonstrate the arithmetic functions</span>

    <span># Most AWK implementations have some standard trig functions</span>
    <span>localvar</span> <span>=</span> <span>sin</span><span>(</span><span>a</span><span>);</span>
    <span>localvar</span> <span>=</span> <span>cos</span><span>(</span><span>a</span><span>);</span>
    <span>localvar</span> <span>=</span> <span>atan2</span><span>(</span><span>b</span><span>,</span> <span>a</span><span>);</span> <span># arc tangent of b / a</span>

    <span># And logarithmic stuff</span>
    <span>localvar</span> <span>=</span> <span>exp</span><span>(</span><span>a</span><span>);</span>
    <span>localvar</span> <span>=</span> <span>log</span><span>(</span><span>a</span><span>);</span>

    <span># Square root</span>
    <span>localvar</span> <span>=</span> <span>sqrt</span><span>(</span><span>a</span><span>);</span>

    <span># Truncate floating point to integer</span>
    <span>localvar</span> <span>=</span> <span>int</span><span>(</span><span>5.34</span><span>);</span> <span># localvar =&gt; 5</span>

    <span># Random numbers</span>
    <span>srand</span><span>();</span> <span># Supply a seed as an argument. By default, it uses the time of day</span>
    <span>localvar</span> <span>=</span> <span>rand</span><span>();</span> <span># Random number between 0 and 1.</span>

    <span># Here's how to return a value</span>
    <span>return</span> <span>localvar</span><span>;</span>
<span>}</span>

<span>function</span> <span>string_functions</span><span>(</span>    <span>localvar</span><span>,</span> <span>arr</span><span>)</span> <span>{</span>

    <span># AWK, being a string-processing language, has several string-related</span>
    <span># functions, many of which rely heavily on regular expressions.</span>

    <span># Search and replace, first instance (sub) or all instances (gsub)</span>
    <span># Both return number of matches replaced</span>
    <span>localvar</span> <span>=</span> <span>"fooooobar"</span><span>;</span>
    <span>sub</span><span>(</span><span>"fo+"</span><span>,</span> <span>"Meet me at the "</span><span>,</span> <span>localvar</span><span>);</span> <span># localvar =&gt; "Meet me at the bar"</span>
    <span>gsub</span><span>(</span><span>"e+"</span><span>,</span> <span>"."</span><span>,</span> <span>localvar</span><span>);</span> <span># localvar =&gt; "m..t m. at th. bar"</span>

    <span># Search for a string that matches a regular expression</span>
    <span># index() does the same thing, but doesn't allow a regular expression</span>
    <span>match</span><span>(</span><span>localvar</span><span>,</span> <span>"t"</span><span>);</span> <span># =&gt; 4, since the 't' is the fourth character</span>

    <span># Split on a delimiter</span>
    <span>n</span> <span>=</span> <span>split</span><span>(</span><span>"foo-bar-baz"</span><span>,</span> <span>arr</span><span>,</span> <span>"-"</span><span>);</span> <span># a[1] = "foo"; a[2] = "bar"; a[3] = "baz"; n = 3</span>

    <span># Other useful stuff</span>
    <span>sprintf</span><span>(</span><span>"%s %d %d %d"</span><span>,</span> <span>"Testing"</span><span>,</span> <span>1</span><span>,</span> <span>2</span><span>,</span> <span>3</span><span>);</span> <span># =&gt; "Testing 1 2 3"</span>
    <span>substr</span><span>(</span><span>"foobar"</span><span>,</span> <span>2</span><span>,</span> <span>3</span><span>);</span> <span># =&gt; "oob"</span>
    <span>substr</span><span>(</span><span>"foobar"</span><span>,</span> <span>4</span><span>);</span> <span># =&gt; "bar"</span>
    <span>length</span><span>(</span><span>"foo"</span><span>);</span> <span># =&gt; 3</span>
    <span>tolower</span><span>(</span><span>"FOO"</span><span>);</span> <span># =&gt; "foo"</span>
    <span>toupper</span><span>(</span><span>"foo"</span><span>);</span> <span># =&gt; "FOO"</span>
<span>}</span>

<span>function</span> <span>io_functions</span><span>(</span>    <span>localvar</span><span>)</span> <span>{</span>

    <span># You've already seen print</span>
    <span>print</span> <span>"Hello world"</span><span>;</span>

    <span># There's also printf</span>
    <span>printf</span><span>(</span><span>"%s %d %d %d\n"</span><span>,</span> <span>"Testing"</span><span>,</span> <span>1</span><span>,</span> <span>2</span><span>,</span> <span>3</span><span>);</span>

    <span># AWK doesn't have file handles, per se. It will automatically open a file</span>
    <span># handle for you when you use something that needs one. The string you used</span>
    <span># for this can be treated as a file handle, for purposes of I/O. This makes</span>
    <span># it feel sort of like shell scripting, but to get the same output, the string</span>
    <span># must match exactly, so use a variable:</span>

    <span>outfile</span> <span>=</span> <span>"/tmp/foobar.txt"</span><span>;</span>

    <span>print</span> <span>"foobar"</span> <span>&gt;</span> <span>outfile</span><span>;</span>

    <span># Now the string outfile is a file handle. You can close it:</span>
    <span>close</span><span>(</span><span>outfile</span><span>);</span>

    <span># Here's how you run something in the shell</span>
    <span>system</span><span>(</span><span>"echo foobar"</span><span>);</span> <span># =&gt; prints foobar</span>

    <span># Reads a line from standard input and stores in localvar</span>
    <span>getline</span> <span>localvar</span><span>;</span>

    <span># Reads a line from a pipe (again, use a string so you close it properly)</span>
    <span>cmd</span> <span>=</span> <span>"echo foobar"</span><span>;</span>
    <span>cmd</span> <span>|</span> <span>getline</span> <span>localvar</span><span>;</span> <span># localvar =&gt; "foobar"</span>
    <span>close</span><span>(</span><span>cmd</span><span>);</span>

    <span># Reads a line from a file and stores in localvar</span>
    <span>infile</span> <span>=</span> <span>"/tmp/foobar.txt"</span><span>;</span>
    <span>getline</span> <span>localvar</span> <span>&lt;</span> <span>infile</span><span>;</span> 
    <span>close</span><span>(</span><span>infile</span><span>);</span>
<span>}</span>

<span># As I said at the beginning, AWK programs consist of a collection of patterns</span>
<span># and actions. You've already seen the BEGIN pattern. Other</span>
<span># patterns are used only if you're processing lines from files or standard</span>
<span># input.</span>
<span>#</span>
<span># When you pass arguments to AWK, they are treated as file names to process.</span>
<span># It will process them all, in order. Think of it like an implicit for loop,</span>
<span># iterating over the lines in these files. these patterns and actions are like</span>
<span># switch statements inside the loop. </span>

<span>/^fo+bar$/</span> <span>{</span>

    <span># This action will execute for every line that matches the regular</span>
    <span># expression, /^fo+bar$/, and will be skipped for any line that fails to</span>
    <span># match it. Let's just print the line:</span>

    <span>print</span><span>;</span>

    <span># Whoa, no argument! That's because print has a default argument: $0.</span>
    <span># $0 is the name of the current line being processed. It is created</span>
    <span># automatically for you.</span>

    <span># You can probably guess there are other $ variables. Every line is</span>
    <span># implicitly split before every action is called, much like the shell</span>
    <span># does. And, like the shell, each field can be access with a dollar sign</span>

    <span># This will print the second and fourth fields in the line</span>
    <span>print</span> <span>$</span><span>2</span><span>,</span> <span>$</span><span>4</span><span>;</span>

    <span># AWK automatically defines many other variables to help you inspect and</span>
    <span># process each line. The most important one is NF</span>

    <span># Prints the number of fields on this line</span>
    <span>print</span> <span>NF</span><span>;</span>

    <span># Print the last field on this line</span>
    <span>print</span> <span>$</span><span>NF</span><span>;</span>
<span>}</span>

<span># Every pattern is actually a true/false test. The regular expression in the</span>
<span># last pattern is also a true/false test, but part of it was hidden. If you</span>
<span># don't give it a string to test, it will assume $0, the line that it's</span>
<span># currently processing. Thus, the complete version of it is this:</span>

<span>$</span><span>0</span> <span>~</span> <span>/^fo+bar$/</span> <span>{</span>
    <span>print</span> <span>"Equivalent to the last pattern"</span><span>;</span>
<span>}</span>

<span>a</span> <span>&gt;</span> <span>0</span> <span>{</span>
    <span># This will execute once for each line, as long as a is positive</span>
<span>}</span>

<span># You get the idea. Processing text files, reading in a line at a time, and</span>
<span># doing something with it, particularly splitting on a delimiter, is so common</span>
<span># in UNIX that AWK is a scripting language that does all of it for you, without</span>
<span># you needing to ask. All you have to do is write the patterns and actions</span>
<span># based on what you expect of the input, and what you want to do with it.</span>

<span># Here's a quick example of a simple script, the sort of thing AWK is perfect</span>
<span># for. It will read a name from standard input and then will print the average</span>
<span># age of everyone with that first name. Let's say you supply as an argument the</span>
<span># name of a this data file:</span>
<span>#</span>
<span># Bob Jones 32</span>
<span># Jane Doe 22</span>
<span># Steve Stevens 83</span>
<span># Bob Smith 29</span>
<span># Bob Barker 72</span>
<span>#</span>
<span># Here's the script:</span>

<span>BEGIN</span> <span>{</span>

    <span># First, ask the user for the name</span>
    <span>print</span> <span>"What name would you like the average age for?"</span><span>;</span>

    <span># Get a line from standard input, not from files on the command line</span>
    <span>getline</span> <span>name</span> <span>&lt;</span> <span>"/dev/stdin"</span><span>;</span>
<span>}</span>

<span># Now, match every line whose first field is the given name</span>
<span>$</span><span>1</span> <span>==</span> <span>name</span> <span>{</span>

    <span># Inside here, we have access to a number of useful variables, already</span>
    <span># pre-loaded for us:</span>
    <span># $0 is the entire line</span>
    <span># $3 is the third field, the age, which is what we're interested in here</span>
    <span># NF is the number of fields, which should be 3</span>
    <span># NR is the number of records (lines) seen so far</span>
    <span># FILENAME is the name of the file being processed</span>
    <span># FS is the field separator being used, which is " " here</span>
    <span># ...etc. There are plenty more, documented in the man page.</span>

    <span># Keep track of a running total and how many lines matched</span>
    <span>sum</span> <span>+=</span> <span>$</span><span>3</span><span>;</span>
    <span>nlines</span><span>++</span><span>;</span>
<span>}</span>

<span># Another special pattern is called END. It will run after processing all the</span>
<span># text files. Unlike BEGIN, it will only run if you've given it input to</span>
<span># process. It will run after all the files have been read and processed</span>
<span># according to the rules and actions you've provided. The purpose of it is</span>
<span># usually to output some kind of final report, or do something with the</span>
<span># aggregate of the data you've accumulated over the course of the script.</span>

<span>END</span> <span>{</span>
    <span>if</span> <span>(</span><span>nlines</span><span>)</span>
        <span>print</span> <span>"The average age for "</span> <span>name</span> <span>" is "</span> <span>sum</span> <span>/</span> <span>nlines</span><span>;</span>
<span>}</span>
</pre><p><span>&nbsp;</span></p></div>]]></description>
	<dc:creator>BioJoker</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43863/snakemake-tutorials</guid>
	<pubDate>Mon, 09 May 2022 05:20:41 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43863/snakemake-tutorials</link>
	<title><![CDATA[Snakemake Tutorials !]]></title>
	<description><![CDATA[<p>A lesson introducing the Snakemake workflow system for bioinformatics analysis.</p>
<blockquote>
<h2 id="prerequisites">Prerequisites<a href="https://carpentries-incubator.github.io/snakemake-novice-bioinformatics/index.html#prerequisites"></a></h2>
<p>This is an intermediate lesson and assumes learners have already done some bioinformatics:</p>
<ul>
<li>Familiarity with the BASH command shell, including concepts like pipes, variables and loops.</li>
<li>Knowledge of bioinformatics fundamentals like the FASTQ file format and transcriptome sequencing, in order to understand the example workflow.</li>
</ul>
<p>No previous knowledge of Snakemake or workflow systems is required.</p>
<p>https://carpentries-incubator.github.io/snakemake-novice-bioinformatics/index.html</p>
</blockquote><p>Address of the bookmark: <a href="https://carpentries-incubator.github.io/snakemake-novice-bioinformatics/aio/index.html" rel="nofollow">https://carpentries-incubator.github.io/snakemake-novice-bioinformatics/aio/index.html</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34246/unicycler-hybrid-assembly-pipeline-for-bacterial-genomes</guid>
	<pubDate>Fri, 10 Nov 2017 03:58:27 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34246/unicycler-hybrid-assembly-pipeline-for-bacterial-genomes</link>
	<title><![CDATA[Unicycler: Hybrid assembly pipeline for bacterial genomes]]></title>
	<description><![CDATA[<p><span>Unicycler is an assembly pipeline for bacterial genomes. It can assemble&nbsp;</span><a href="http://www.illumina.com/">Illumina</a><span>-only read sets where it functions as a&nbsp;</span><a href="http://cab.spbu.ru/software/spades/">SPAdes</a><span>-optimiser. It can also assembly long-read-only sets (</span><a href="http://www.pacb.com/">PacBio</a><span>&nbsp;or&nbsp;</span><a href="https://nanoporetech.com/">Nanopore</a><span>) where it runs a&nbsp;</span><a href="https://github.com/lh3/miniasm">miniasm</a><span>+</span><a href="https://github.com/isovic/racon">Racon</a><span>&nbsp;pipeline. For the best possible assemblies, give it both Illumina reads&nbsp;</span><em>and</em><span>&nbsp;long reads, and it will conduct a hybrid assembly.</span></p><p>Address of the bookmark: <a href="https://github.com/rrwick/Unicycler" rel="nofollow">https://github.com/rrwick/Unicycler</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/36621/hapcut2-robust-and-accurate-haplotype-assembly-for-diverse-sequencing-technologies</guid>
	<pubDate>Tue, 15 May 2018 07:35:26 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/36621/hapcut2-robust-and-accurate-haplotype-assembly-for-diverse-sequencing-technologies</link>
	<title><![CDATA[HapCUT2: robust and accurate haplotype assembly for diverse sequencing technologies]]></title>
	<description><![CDATA[HapCUT2 is a maximum-likelihood-based tool for assembling haplotypes from DNA sequence reads, designed to "just work" with excellent speed and accuracy. We found that previously described haplotype assembly methods are specialized for specific read technologies or protocols, with slow or inaccurate performance on others. With this in mind, HapCUT2 is designed for speed and accuracy across diverse sequencing technologies, including but not limited to:

NGS short reads (Illumina HiSeq)
clone-based sequencing (Fosmid or BAC clones)
SMRT reads (PacBio)
Oxford Nanopore reads
10X Genomics Linked-Reads
proximity-ligation (Hi-C) reads
high-coverage sequencing (&gt;40x coverage-per-SNP) using above technologies
combinations of the above technologies (e.g. scaffold long reads with Hi-C reads)
See below for specific examples of command line options and best practices for some of these technologies.

NOTE: At this time HapCUT2 is for diploid organisms only. VCF input should contain diploid variants.

If you use HapCUT2 in your research, please cite:

Edge, P., Bafna, V. &amp; Bansal, V. HapCUT2: robust and accurate haplotype assembly for diverse sequencing technologies. Genome Res. gr.213462.116 (2016). doi:10.1101/gr.213462.116<p>Address of the bookmark: <a href="https://github.com/vibansal/HapCUT2" rel="nofollow">https://github.com/vibansal/HapCUT2</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37291/transrate-understanding-your-transcriptome-assembly</guid>
	<pubDate>Fri, 13 Jul 2018 07:49:26 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37291/transrate-understanding-your-transcriptome-assembly</link>
	<title><![CDATA[transrate: Understanding your transcriptome assembly]]></title>
	<description><![CDATA[<p><span>Transrate is software for&nbsp;</span><em>de-novo</em><span>&nbsp;transcriptome assembly quality analysis. It examines your assembly in detail and compares it to experimental evidence such as the sequencing reads, reporting quality scores for contigs and assemblies. This allows you to choose between assemblers and parameters, filter out the bad contigs from an assembly, and help decide when to stop trying to improve the assembly.</span></p><p>Address of the bookmark: <a href="http://hibberdlab.com/transrate/index.html" rel="nofollow">http://hibberdlab.com/transrate/index.html</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>

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