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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/38304?offset=410</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38199/pacasus-correction-of-palindromes-in-long-reads-from-pacbio-and-nanopore</guid>
	<pubDate>Mon, 12 Nov 2018 05:26:48 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38199/pacasus-correction-of-palindromes-in-long-reads-from-pacbio-and-nanopore</link>
	<title><![CDATA[Pacasus: Correction of palindromes in long reads from PacBio and Nanopore]]></title>
	<description><![CDATA[<p><br>Tool for detecting and cleaning PacBio / Nanopore long reads after whole genome amplification. Check the poster from the Revolutionizing Next-Generation Sequencing (2nd edition) conference in the source folder:&nbsp;<a href="https://github.com/swarris/Pacasus/blob/master/vib2017.pdf">https://github.com/swarris/Pacasus/blob/master/vib2017.pdf</a>.</p>
<p>The prepint version is found on&nbsp;<a href="http://www.biorxiv.org/content/early/2017/08/09/173872">http://www.biorxiv.org/content/early/2017/08/09/173872</a></p>
<p>It uses the pyPaSWAS framework for sequence alignment (<a href="https://github.com/swarris/pyPaSWAS">https://github.com/swarris/pyPaSWAS</a>)</p><p>Address of the bookmark: <a href="https://github.com/swarris/Pacasus" rel="nofollow">https://github.com/swarris/Pacasus</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40516/nextdenovo-string-graph-based-de-novo-assembler-for-tgs-long-reads</guid>
	<pubDate>Sun, 05 Jan 2020 04:08:29 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40516/nextdenovo-string-graph-based-de-novo-assembler-for-tgs-long-reads</link>
	<title><![CDATA[NextDenovo: string graph-based de novo assembler for TGS long reads]]></title>
	<description><![CDATA[<p>NextDenovo is a string graph-based<span>&nbsp;</span><em>de novo</em><span>&nbsp;</span>assembler for TGS long reads. It uses a "correct-then-assemble" strategy similar to canu, but requires significantly less computing resources and storages. After assembly, the per-base error rate is about 97-98%, to further improve single base accuracy, please use<span>&nbsp;</span><a href="https://github.com/Nextomics/NextPolish">NextPolish</a>.</p>
<p>NextDenovo contains two core modules: NextCorrect and NextGraph. NextCorrect can be used to correct TGS long reads with approximately 15% sequencing errors, and NextGraph can be used to construct a string graph with corrected reads. It also contains a modified version of<span>&nbsp;</span><a href="https://github.com/lh3/minimap2">minimap2</a><span>&nbsp;</span>for adapting input and output and producing more sensitive and accurate dovetail overlaps, and some useful utilities (see<span>&nbsp;</span><a href="https://github.com/Nextomics/NextDenovo/blob/master/doc/UTILITY.md">here</a><span>&nbsp;</span>for more details).</p><p>Address of the bookmark: <a href="https://github.com/Nextomics/NextDenovo" rel="nofollow">https://github.com/Nextomics/NextDenovo</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41501/hicanu-accurate-assembly-of-segmental-duplications-satellites-and-allelic-variants-from-high-fidelity-long-reads</guid>
	<pubDate>Fri, 27 Mar 2020 22:49:31 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41501/hicanu-accurate-assembly-of-segmental-duplications-satellites-and-allelic-variants-from-high-fidelity-long-reads</link>
	<title><![CDATA[HiCanu: accurate assembly of segmental duplications, satellites, and allelic variants from high-fidelity long reads]]></title>
	<description><![CDATA[<p><span>HiCanu, a significant modification of the Canu assembler designed to leverage the full potential of HiFi reads via homopolymer compression, overlap-based error correction, and aggressive false overlap filtering.&nbsp;</span></p>
<p>More at&nbsp;<a href="https://www.biorxiv.org/content/10.1101/2020.03.14.992248v3?fbclid=IwAR2PaN4GLjvAZpWmCE2q0EWk2dtwY7wiKxVlXn9PPG7OBSP06PP2gcCrv3A">https://www.biorxiv.org/content/10.1101/2020.03.14.992248v3</a></p><p>Address of the bookmark: <a href="https://github.com/marbl/canu" rel="nofollow">https://github.com/marbl/canu</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31137/finishersc-a-repeat-aware-and-scalable-tool-for-upgrading-de-novo-assembly-using-long-reads</guid>
	<pubDate>Mon, 27 Feb 2017 09:49:45 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31137/finishersc-a-repeat-aware-and-scalable-tool-for-upgrading-de-novo-assembly-using-long-reads</link>
	<title><![CDATA[FinisherSC: a repeat-aware and scalable tool for upgrading de novo assembly using long reads]]></title>
	<description><![CDATA[<p><span>FinisherSC, a repeat-aware and scalable tool for upgrading&nbsp;</span><em>de novo</em><span>&nbsp;assembly using long reads. Experiments with real data suggest that FinisherSC can provide longer and higher quality contigs than existing tools while maintaining high concordance.</span></p><p>Address of the bookmark: <a href="http://kakitone.github.io/finishingTool/" rel="nofollow">http://kakitone.github.io/finishingTool/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32190/dbg2olcefficient-assembly-of-large-genomes-using-long-erroneous-reads-of-the-third-generation-sequencing-technologies</guid>
	<pubDate>Wed, 19 Apr 2017 10:09:51 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32190/dbg2olcefficient-assembly-of-large-genomes-using-long-erroneous-reads-of-the-third-generation-sequencing-technologies</link>
	<title><![CDATA[DBG2OLC:Efficient Assembly of Large Genomes Using Long Erroneous Reads of the Third Generation Sequencing Technologies]]></title>
	<description><![CDATA[<p>DBG2OLC:Efficient Assembly of Large Genomes Using Long Erroneous Reads of the Third Generation Sequencing Technologies</p>
<p>Our work is published in Scientific Reports:</p>
<p>Ye, C. et al. DBG2OLC: Efficient Assembly of Large Genomes Using Long Erroneous Reads of the Third Generation Sequencing Technologies. Sci. Rep. 6, 31900; doi: 10.1038/srep31900 (2016).</p>
<p><a href="http://www.nature.com/articles/srep31900">http://www.nature.com/articles/srep31900</a></p>
<p>The manual can be downloaded from:</p>
<p><a href="https://github.com/yechengxi/DBG2OLC/raw/master/Manual.docx">https://github.com/yechengxi/DBG2OLC/raw/master/Manual.docx</a></p>
<p>To use precompiled versions,please go to:</p>
<p><a href="https://github.com/yechengxi/DBG2OLC/tree/master/compiled">https://github.com/yechengxi/DBG2OLC/tree/master/compiled</a></p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="https://github.com/yechengxi/DBG2OLC" rel="nofollow">https://github.com/yechengxi/DBG2OLC</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37563/colormap-correcting-long-reads-by-mapping-short-reads</guid>
	<pubDate>Mon, 20 Aug 2018 14:17:05 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37563/colormap-correcting-long-reads-by-mapping-short-reads</link>
	<title><![CDATA[CoLoRMap: Correcting Long Reads by Mapping short reads]]></title>
	<description><![CDATA[<p><span>Second generation sequencing technologies paved the way to an exceptional increase in the number of sequenced genomes, both prokaryotic and eukaryotic. However, short reads are difficult to assemble and often lead to highly fragmented assemblies. The recent developments in long reads sequencing methods offer a promising way to address this issue. However, so far long reads are characterized by a high error rate, and assembling from long reads require a high depth of coverage. This motivates the development of hybrid approaches that leverage the high quality of short reads to correct errors in long reads.We introduce CoLoRMap, a hybrid method for correcting noisy long reads, such as the ones produced by PacBio sequencing technology, using high-quality Illumina paired-end reads mapped onto the long reads. Our algorithm is based on two novel ideas: using a classical shortest path algorithm to find a sequence of overlapping short reads that minimizes the edit score to a long read and extending corrected regions by local assembly of unmapped mates of mapped short reads. Our results on bacterial, fungal and insect data sets show that CoLoRMap compares well with existing hybrid correction methods.The source code of CoLoRMap is freely available for non-commercial use at https://github.com/sfu-compbio/colormap</span></p>
<p><span>ehaghshe@sfu.ca or cedric.chauve@sfu.ca</span></p><p>Address of the bookmark: <a href="https://github.com/sfu-compbio/colormap" rel="nofollow">https://github.com/sfu-compbio/colormap</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43048/coverm-read-coverage-calculator-for-metagenomics</guid>
	<pubDate>Thu, 29 Apr 2021 23:39:14 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43048/coverm-read-coverage-calculator-for-metagenomics</link>
	<title><![CDATA[CoverM: Read coverage calculator for metagenomics]]></title>
	<description><![CDATA[<p>CoverM aims to be a configurable, easy to use and fast DNA read coverage and relative abundance calculator focused on metagenomics applications.</p>
<p>CoverM calculates coverage of genomes/MAGs&nbsp;<code>coverm genome</code>&nbsp;(<a href="https://wwood.github.io/CoverM/coverm-genome.html">help</a>) or individual contigs&nbsp;<code>coverm contig</code>&nbsp;(<a href="https://wwood.github.io/CoverM/coverm-contig.html">help</a>). Calculating coverage by read mapping, its input can either be BAM files sorted by reference, or raw reads and reference genomes in various formats.</p><p>Address of the bookmark: <a href="https://github.com/wwood/CoverM" rel="nofollow">https://github.com/wwood/CoverM</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/35061/proovread-large-scale-high-accuracy-pacbio-correction-through-iterative-short-read-consensus</guid>
	<pubDate>Fri, 05 Jan 2018 04:12:20 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/35061/proovread-large-scale-high-accuracy-pacbio-correction-through-iterative-short-read-consensus</link>
	<title><![CDATA[proovread : large-scale high-accuracy PacBio correction through iterative short read consensus]]></title>
	<description><![CDATA[<p>proovread : large-scale high-accuracy PacBio correction through iterative short read consensus</p>
<ul>
<li>outperforms PacBioToCA/LSC in terms of accuracy and contiguity/sensitivity (<a href="http://dx.doi.org/10.1093/bioinformatics/btu392">http://dx.doi.org/10.1093/bioinformatics/btu392</a>)</li>
<li>is easy to install/run/configure</li>
<li>supports various types of dat
<ul>
<li><strong>HiSeq/MiSeq&nbsp;</strong>(100-500bp)</li>
<li><strong>Unitigs</strong></li>
<li>454, ...</li>
</ul>
</li>
</ul>
<p>proovread maps high coverage data to pacbio reads (bwa mem, blasr, daligner) in multiple iterations.</p><p>Address of the bookmark: <a href="https://github.com/BioInf-Wuerzburg/proovread" rel="nofollow">https://github.com/BioInf-Wuerzburg/proovread</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/42280/urmap-an-ultra-fast-read-mapper</guid>
	<pubDate>Thu, 29 Oct 2020 23:03:54 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/42280/urmap-an-ultra-fast-read-mapper</link>
	<title><![CDATA[URMAP, an ultra-fast read mapper]]></title>
	<description><![CDATA[<p><span>URMAP, a new read mapping algorithm. URMAP is an order of magnitude faster than BWA with comparable accuracy on several validation tests. On a Genome in a Bottle (GIAB) variant calling test with 30&times; coverage 2&times;150 reads, URMAP achieves high accuracy (precision 0.998, sensitivity 0.982 and F-measure 0.990) with the strelka2 caller. However, GIAB reference variants are shown to be biased against repetitive regions which are difficult to map and may therefore pose an unrealistically easy challenge to read mappers and variant callers.</span></p>
<p><span>More at&nbsp;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320720/</span></p><p>Address of the bookmark: <a href="https://github.com/rcedgar/urmap" rel="nofollow">https://github.com/rcedgar/urmap</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/9639/find-certain-filesdocuments-in-linux-os</guid>
	<pubDate>Sun, 06 Apr 2014 23:56:18 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/9639/find-certain-filesdocuments-in-linux-os</link>
	<title><![CDATA[Find certain files/documents in Linux OS]]></title>
	<description><![CDATA[<p>As bioinformatician I know the fact that we usually handle the large dataset and lost in the huge numbers of files and folders. In order to search the missing file a strong search command is required. The Linux Find Command is one of the most important and much used command in Linux sytems. Find command used to search and locate list of files and directories based on conditions you specify for files that match the arguments. Find can be used in variety of conditions like you can find files by permissions, users, groups, file type, date, size and other possible criteria.<br /><br />Through this article we are sharing our day-to-day Linux find command experience and its usage in the form of examples. In this article we will show you the most used 35 Find Commands examples in Linux. We have divided the section into Five parts from basic to advance usage of find command.</p><p><strong>Part I &ndash; Basic Find Commands for Finding Files with Names</strong><br />1. Find Files Using Name in Current Directory<br /><br />Find all the files whose name is gene.txt in a current working directory.<br /><br /># find . -name gene.txt<br /><br />./gene.txt<br /><br />2. Find Files Under Home Directory<br /><br />Find all the files under /home directory with name gene.txt.<br /><br /># find /home -name gene.txt<br /><br />/home/gene.txt<br /><br />3. Find Files Using Name and Ignoring Case<br /><br />Find all the files whose name is gene.txt and contains both capital and small letters in /home directory.<br /><br /># find /home -iname gene.txt<br /><br />./gene.txt<br />./Gene.txt<br /><br />4. Find Directories Using Name<br /><br />Find all directories whose name is Gene in / directory.<br /><br /># find / -type d -name Gene<br /><br />/Gene<br /><br />5. Find fasta Files Using Name<br /><br />Find all php files whose name is gene.fasta in a current working directory.<br /><br /># find . -type f -name gene.fasta<br /><br />./gene.fasta<br /><br />6. Find all PHP Files in Directory<br /><br />Find all fasta files in a directory.<br /><br /># find . -type f -name "*.fasta"<br /><br />./gene.fasta<br />./cancer.fasta<br />./allgene.fasta<br /><br /><strong>Part II &ndash; Find Files Based on their Permissions</strong><br />7. Find Files With 777 Permissions<br /><br />Find all the files whose permissions are 777.<br /><br /># find . -type f -perm 0777 -print<br /><br />8. Find Files Without 777 Permissions<br /><br />Find all the files without permission 777.<br /><br /># find / -type f ! -perm 777<br /><br />9. Find SGID Files with 644 Permissions<br /><br />Find all the SGID bit files whose permissions set to 644.<br /><br /># find / -perm 2644<br /><br />10. Find Sticky Bit Files with 551 Permissions<br /><br />Find all the Sticky Bit set files whose permission are 551.<br /><br /># find / -perm 1551<br /><br />11. Find SUID Files<br /><br />Find all SUID set files.<br /><br /># find / -perm /u=s<br /><br />12. Find SGID Files<br /><br />Find all SGID set files.<br /><br /># find / -perm /g+s<br /><br />13. Find Read Only Files<br /><br />Find all Read Only files.<br /><br /># find / -perm /u=r<br /><br />14. Find Executable Files<br /><br />Find all Executable files.<br /><br /># find / -perm /a=x<br /><br />15. Find Files with 777 Permissions and Chmod to 644<br /><br />Find all 777 permission files and use chmod command to set permissions to 644.<br /><br /># find / -type f -perm 0777 -print -exec chmod 644 {} \;<br /><br />16. Find Directories with 777 Permissions and Chmod to 755<br /><br />Find all 777 permission directories and use chmod command to set permissions to 755.<br /><br /># find / -type d -perm 777 -print -exec chmod 755 {} \;<br /><br />17. Find and remove single File<br /><br />To find a single file called gene.txt and remove it.<br /><br /># find . -type f -name "gene.txt" -exec rm -f {} \;<br /><br />18. Find and remove Multiple File<br /><br />To find and remove multiple files such as .fa or .gb, then use.<br /><br /># find . -type f -name "*.fa" -exec rm -f {} \;<br /><br />OR<br /><br /># find . -type f -name "*.gb" -exec rm -f {} \;<br /><br />19. Find all Empty Files<br /><br />To file all empty files under certain path.<br /><br /># find /tmp -type f -empty<br /><br />20. Find all Empty Directories<br /><br />To file all empty directories under certain path.<br /><br /># find /tmp -type d -empty<br /><br />21. File all Hidden Files<br /><br />To find all hidden files, use below command.<br /><br /># find /tmp -type f -name ".*"<br /><br /><strong>Part III &ndash; Search Files Based On Owners and Groups</strong><br />22. Find Single File Based on User<br /><br />To find all or single file called gene.txt under / root directory of owner root.<br /><br /># find / -user root -name gene.txt<br /><br />23. Find all Files Based on User<br /><br />To find all files that belongs to user Rahul under /home directory.<br /><br /># find /home -user rahul<br /><br />24. Find all Files Based on Group<br /><br />To find all files that belongs to group Developer under /home directory.<br /><br /># find /home -group developer<br /><br />25. Find Particular Files of User<br /><br />To find all .txt files of user Rahul under /home directory.<br /><br /># find /home -user rahul -iname "*.txt"<br /><br /><strong>Part IV &ndash; Find Files and Directories Based on Date and Time</strong><br />26. Find Last 50 Days Modified Files<br /><br />To find all the files which are modified 50 days back.<br /><br /># find / -mtime 50<br /><br />27. Find Last 50 Days Accessed Files<br /><br />To find all the files which are accessed 50 days back.<br /><br /># find / -atime 50<br /><br />28. Find Last 50-100 Days Modified Files<br /><br />To find all the files which are modified more than 50 days back and less than 100 days.<br /><br /># find / -mtime +50 &ndash;mtime -100<br /><br />29. Find Changed Files in Last 1 Hour<br /><br />To find all the files which are changed in last 1 hour.<br /><br /># find / -cmin -60<br /><br />30. Find Modified Files in Last 1 Hour<br /><br />To find all the files which are modified in last 1 hour.<br /><br /># find / -mmin -60<br /><br />31. Find Accessed Files in Last 1 Hour<br /><br />To find all the files which are accessed in last 1 hour.<br /><br /># find / -amin -60<br /><br /><strong>Part V &ndash; Find Files and Directories Based on Size</strong><br />32. Find 50MB Files<br /><br />To find all 50MB files, use.<br /><br /># find / -size 50M<br /><br />33. Find Size between 50MB &ndash; 100MB<br /><br />To find all the files which are greater than 50MB and less than 100MB.<br /><br /># find / -size +50M -size -100M<br /><br />34. Find and Delete 100MB Files<br /><br />To find all 100MB files and delete them using one single command.<br /><br /># find / -size +100M -exec rm -rf {} \;<br /><br />35. Find Specific Files and Delete<br /><br />Find all .gb files with more than 10MB and delete them using one single command.<br /><br /># find / -type f -name *.gb -size +10M -exec rm {} \;</p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>

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