<![CDATA[BOL: Related items]]> https://bioinformaticsonline.com/related/38668?offset=470 https://bioinformaticsonline.com/bookmarks/view/39872/miropeats-discovers-regions-of-sequence-similarity-amongst-any-set-of-dna-sequences Mon, 26 Aug 2019 17:55:24 -0500 https://bioinformaticsonline.com/bookmarks/view/39872/miropeats-discovers-regions-of-sequence-similarity-amongst-any-set-of-dna-sequences <![CDATA[Miropeats: discovers regions of sequence similarity amongst any set of DNA sequences]]> Miropeats discovers regions of sequence similarity amongst any set of DNA sequences and then presents this similarity information graphically. Sequence similarity searching is a very general tool that forms the basis of many different biological sequence analyses but it is limited by the verbosity of traditional alignment presentation styles. Miropeats enhances the utility of conventional DNA sequence comparisons when looking at long lengths of sequence similarity by summarizing extensive large scale sequence similarities on a single page of graphics. The latest version of Miropeats can be used as a general pairwise alignment program or in its traditional role sorting out a big mess of overlapping or similar regions.

Address of the bookmark: http://www.littlest.co.uk/software/bioinf/old_packages/miropeats/

]]> Poonam Mahapatra https://bioinformaticsonline.com/bookmarks/view/41602/nucdiff-in-depth-characterization-and-annotation-of-differences-between-two-sets-of-dna-sequences Tue, 05 May 2020 10:35:48 -0500 https://bioinformaticsonline.com/bookmarks/view/41602/nucdiff-in-depth-characterization-and-annotation-of-differences-between-two-sets-of-dna-sequences <![CDATA[NucDiff: In-depth characterization and annotation of differences between two sets of DNA sequences]]> NucDiff locates and categorizes differences between two closely related nucleotide sequences. It is able to deal with very fragmented genomes, structural rearrangements and various local differences. These features make NucDiff to be perfectly suitable to compare assemblies with each other or with available reference genomes.

NucDiff provides information about the types of differences and their locations. It is possible to upload the results into genome browser for visualization and further inspection. It was written in Python and uses the NUCmer package from MUMmer[1] for sequence comparison.



Address of the bookmark: https://github.com/uio-cels/NucDiff

]]> Jit https://bioinformaticsonline.com/blog/view/44227/common-methods-to-discover-tandem-repeats Thu, 09 Mar 2023 02:40:52 -0600 https://bioinformaticsonline.com/blog/view/44227/common-methods-to-discover-tandem-repeats <![CDATA[Common methods to discover tandem repeats]]>

Tandem repeats are DNA sequences that are repeated in a contiguous manner in the genome. These sequences are often used as genetic markers and are important in many areas of genetics and genomics research. Here are some methods for discovering tandem repeats in genomes:

  1. Tandem Repeat Finder: Tandem Repeat Finder is a software tool that identifies tandem repeats in DNA sequences. It is available for free download and can be used on both nucleotide and protein sequences. The tool uses a statistical algorithm to identify repeats based on their length, copy number, and overall composition.

  2. RepeatMasker: RepeatMasker is another software tool that can identify tandem repeats in DNA sequences. It works by comparing the input sequence to a database of known repeats and then identifies any tandem repeats that match those in the database.

  3. PCR-based methods: Polymerase chain reaction (PCR) can be used to amplify and detect tandem repeats in genomic DNA. PCR primers are designed to flank the tandem repeat region, and amplification of the target DNA fragment can be visualized on a gel. This method can be useful for detecting novel tandem repeats and for genotyping.

  4. Southern blotting: Southern blotting is a classic method for detecting DNA fragments in a sample. It can be used to detect tandem repeats by digesting genomic DNA with a restriction enzyme, separating the fragments by gel electrophoresis, and then probing the blot with a tandem repeat-specific probe.

Overall, a combination of these methods can be used to comprehensively identify tandem repeats in genomes.

]]> BioStar https://bioinformaticsonline.com/bookmarks/view/44663/svbyeye-r-package-to-visualize-alignments-between-two-or-multiple-dna-sequences Tue, 17 Sep 2024 02:34:57 -0500 https://bioinformaticsonline.com/bookmarks/view/44663/svbyeye-r-package-to-visualize-alignments-between-two-or-multiple-dna-sequences <![CDATA[SVbyEye: R Package to visualize alignments between two or multiple DNA sequences]]> R Package to visualize alignments between two or multiple DNA sequences including
a number of functionalities to facilitate processing of alignments in PAF format.

SVbyEye, an open-source R package to visualize and annotate sequence-to-sequence alignments along with various functionalities to process alignments in PAF format. The tool facilitates the characterization of complex SVs in the context of sequence homology helping resolve the mechanisms underlying their formation. Availability and implementation SVbyEye is available at https://github.com/daewoooo/SVbyEye.

Author: David Porubsky

Address of the bookmark: https://github.com/daewoooo/SVbyEye

]]> LEGE https://bioinformaticsonline.com/blog/view/23584/integrated-mrna-and-microrna-transcriptome-analysis-in-strand-ngs Tue, 04 Aug 2015 05:04:46 -0500 https://bioinformaticsonline.com/blog/view/23584/integrated-mrna-and-microrna-transcriptome-analysis-in-strand-ngs <![CDATA[Integrated mRNA and microRNA transcriptome analysis in Strand NGS]]> Using a nasopharyngeal carcinoma case study, this paper highlights the integrated transcriptome analysis capabilities of Strand NGS demonstrating the identification of miRNA – mRNA interactions in regulatory networks.
Read the application note on Integrated mRNA and microRNA transcriptome analysis in Strand NGS by Veena Hedatale and Rohit Gupta. For more information, please contact us

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