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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/39200?offset=130</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/33869/import-r-data</guid>
	<pubDate>Wed, 12 Jul 2017 08:30:46 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/33869/import-r-data</link>
	<title><![CDATA[Import R Data]]></title>
	<description><![CDATA[<p>It is often necessary to import sample textbook data into R before you start working on your homework.</p><div id="node-69"><div><p><strong>Excel File</strong></p><p>Quite frequently, the sample data is in&nbsp;<span>Excel&nbsp;</span>format, and needs to be imported into R prior to use. For this, we can use the function&nbsp;<span>read.xls&nbsp;</span>from the&nbsp;<span>gdata&nbsp;</span>package. It reads from an Excel spreadsheet and returns a&nbsp;<a href="http://www.r-tutor.com/r-introduction/data-frame">data frame</a>. The following shows how to load an Excel spreadsheet named&nbsp;<span>"mydata.xls"</span>. This method requires Perl runtime to be present in the system.</p><blockquote><div id="listing-68"><span><a></a></span>&gt;&nbsp;library(gdata)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;#&nbsp;load&nbsp;gdata&nbsp;package&nbsp;<br /><span><a></a></span>&gt;&nbsp;help(read.xls)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;#&nbsp;documentation&nbsp;<br /><span><a></a></span>&gt;&nbsp;mydata&nbsp;=&nbsp;read.xls("mydata.xls")&nbsp;&nbsp;#&nbsp;read&nbsp;from&nbsp;first&nbsp;sheet</div></blockquote><p>Alternatively, we can use the function&nbsp;<span>loadWorkbook&nbsp;</span>from the&nbsp;<span>XLConnect&nbsp;</span>package to read the entire workbook, and then load the worksheets with&nbsp;<span>readWorksheet</span>. The&nbsp;<span>XLConnect&nbsp;</span>package requires Java to be pre-installed.</p><blockquote><div id="listing-69"><span><a></a></span>&gt;&nbsp;library(XLConnect)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;#&nbsp;load&nbsp;XLConnect&nbsp;package&nbsp;<br /><span><a></a></span>&gt;&nbsp;wk&nbsp;=&nbsp;loadWorkbook("mydata.xls")&nbsp;<br /><span><a></a></span>&gt;&nbsp;df&nbsp;=&nbsp;readWorksheet(wk,&nbsp;sheet="Sheet1")</div></blockquote><p>&nbsp;</p><h4><a></a>Minitab File</h4><p>If the data file is in&nbsp;<span>Minitab Portable Worksheet&nbsp;</span>format, it can be opened with the function&nbsp;<span>read.mtp&nbsp;</span>from the&nbsp;<span>foreign&nbsp;</span>package. It returns a&nbsp;<a href="http://www.r-tutor.com/r-introduction/list">list</a>&nbsp;of components in the Minitab worksheet.</p><blockquote><div id="listing-70"><span><a></a></span>&gt;&nbsp;library(foreign)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;#&nbsp;load&nbsp;the&nbsp;foreign&nbsp;package&nbsp;<br /><span><a></a></span>&gt;&nbsp;help(read.mtp)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;#&nbsp;documentation&nbsp;<br /><span><a></a></span>&gt;&nbsp;mydata&nbsp;=&nbsp;read.mtp("mydata.mtp")&nbsp;&nbsp;#&nbsp;read&nbsp;from&nbsp;.mtp&nbsp;file</div></blockquote><p>&nbsp;</p><h4><a></a>SPSS File</h4><p>For the data files in&nbsp;<span>SPSS&nbsp;</span>format, it can be opened with the function&nbsp;<span>read.spss&nbsp;</span>also from the&nbsp;<span>foreign&nbsp;</span>package. There is a&nbsp;<span>"to.data.frame"&nbsp;</span>option for choosing whether a data frame is to be returned. By default, it returns a list of components instead.</p><blockquote><div id="listing-71"><span><a></a></span>&gt;&nbsp;library(foreign)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;#&nbsp;load&nbsp;the&nbsp;foreign&nbsp;package&nbsp;<br /><span><a></a></span>&gt;&nbsp;help(read.spss)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;#&nbsp;documentation&nbsp;<br /><span><a></a></span>&gt;&nbsp;mydata&nbsp;=&nbsp;read.spss("myfile",&nbsp;to.data.frame=TRUE)</div></blockquote><p>&nbsp;</p><h4><a></a>Table File</h4><p>A data table can resides in a text file. The cells inside the table are separated by blank characters. Here is an example of a table with 4 rows and 3 columns.</p><blockquote><div id="listing-72"><span><a></a></span>100&nbsp;&nbsp;&nbsp;a1&nbsp;&nbsp;&nbsp;b1&nbsp;<br /><span><a></a></span>200&nbsp;&nbsp;&nbsp;a2&nbsp;&nbsp;&nbsp;b2&nbsp;<br /><span><a></a></span>300&nbsp;&nbsp;&nbsp;a3&nbsp;&nbsp;&nbsp;b3&nbsp;<br /><span><a></a></span>400&nbsp;&nbsp;&nbsp;a4&nbsp;&nbsp;&nbsp;b4</div></blockquote><p>Now copy and paste the table above in a file named&nbsp;<span>"mydata.txt"&nbsp;</span>with a text editor. Then load the data into the workspace with the function&nbsp;<span>read.table</span>.</p><blockquote><div id="listing-73"><span><a></a></span>&gt;&nbsp;mydata&nbsp;=&nbsp;read.table("mydata.txt")&nbsp;&nbsp;#&nbsp;read&nbsp;text&nbsp;file&nbsp;<br /><span><a></a></span>&gt;&nbsp;mydata&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;#&nbsp;print&nbsp;data&nbsp;frame&nbsp;<br /><span><a></a></span>&nbsp;&nbsp;&nbsp;V1&nbsp;V2&nbsp;V3&nbsp;<br /><span><a></a></span>1&nbsp;100&nbsp;a1&nbsp;b1&nbsp;<br /><span><a></a></span>2&nbsp;200&nbsp;a2&nbsp;b2&nbsp;<br /><span><a></a></span>3&nbsp;300&nbsp;a3&nbsp;b3&nbsp;<br /><span><a></a></span>4&nbsp;400&nbsp;a4&nbsp;b4</div></blockquote><p>For further detail of the function&nbsp;<span>read.table</span>, please consult the R documentation.</p><blockquote><div id="listing-74"><span><a></a></span>&gt;&nbsp;help(read.table)</div></blockquote><p>&nbsp;</p><h4><a></a>CSV File</h4><p>The sample data can also be in&nbsp;<span>comma separated values&nbsp;</span>(CSV) format. Each cell inside such data file is separated by a special character, which usually is a comma, although other characters can be used as well.</p><p>The first row of the data file should contain the column names instead of the actual data. Here is a sample of the expected format.</p><blockquote><div id="listing-75"><span><a></a></span>Col1,Col2,Col3&nbsp;<br /><span><a></a></span>100,a1,b1&nbsp;<br /><span><a></a></span>200,a2,b2&nbsp;<br /><span><a></a></span>300,a3,b3</div></blockquote><p>After we copy and paste the data above in a file named&nbsp;<span>"mydata.csv"&nbsp;</span>with a text editor, we can read the data with the function&nbsp;<span>read.csv</span>.</p><blockquote><div id="listing-76"><span><a></a></span>&gt;&nbsp;mydata&nbsp;=&nbsp;read.csv("mydata.csv")&nbsp;&nbsp;#&nbsp;read&nbsp;csv&nbsp;file&nbsp;<br /><span><a></a></span>&gt;&nbsp;mydata&nbsp;<br /><span><a></a></span>&nbsp;&nbsp;Col1&nbsp;Col2&nbsp;Col3&nbsp;<br /><span><a></a></span>1&nbsp;&nbsp;100&nbsp;&nbsp;&nbsp;a1&nbsp;&nbsp;&nbsp;b1&nbsp;<br /><span><a></a></span>2&nbsp;&nbsp;200&nbsp;&nbsp;&nbsp;a2&nbsp;&nbsp;&nbsp;b2&nbsp;<br /><span><a></a></span>3&nbsp;&nbsp;300&nbsp;&nbsp;&nbsp;a3&nbsp;&nbsp;&nbsp;b3</div></blockquote><p>In various European locales, as the comma character serves as the decimal point, the function&nbsp;<span>read.csv2&nbsp;</span>should be used instead. For further detail of the&nbsp;<span>read.csv&nbsp;</span>and&nbsp;<span>read.csv2&nbsp;</span>functions, please consult the R documentation.</p><blockquote><div id="listing-77"><span><a></a></span>&gt;&nbsp;help(read.csv)</div></blockquote><p>&nbsp;</p><h4><a></a>Working Directory</h4><p>Finally, the code samples above assume the data files are located in the R&nbsp;<span>working</span>&nbsp;<span>directory</span>, which can be found with the function&nbsp;<span>getwd</span>.</p><blockquote><div id="listing-78"><span><a></a></span>&gt;&nbsp;getwd()&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;#&nbsp;get&nbsp;current&nbsp;working&nbsp;directory</div></blockquote><p>You can select a different working directory with the function&nbsp;<span>setwd()</span>, and thus avoid entering the full path of the data files.</p><blockquote><div id="listing-79"><span><a></a></span>&gt;&nbsp;setwd("")&nbsp;&nbsp;&nbsp;#&nbsp;set&nbsp;working&nbsp;directory</div></blockquote><p>Note that the forward slash should be used as the path separator even on Windows platform.</p><blockquote><div id="listing-80"><span><a></a></span>&gt;&nbsp;setwd("C:/MyDoc")</div></blockquote></div></div>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/38226/ncbi-to-assist-in-virus-hunting-data-science-hackathon</guid>
	<pubDate>Thu, 15 Nov 2018 12:55:01 -0600</pubDate>
	<link>https://bioinformaticsonline.com/news/view/38226/ncbi-to-assist-in-virus-hunting-data-science-hackathon</link>
	<title><![CDATA[NCBI to assist in Virus Hunting Data Science Hackathon]]></title>
	<description><![CDATA[<p>NCBI Hackathon are pleased to announce the second installment of the&nbsp;<a href="https://ncbiinsights.ncbi.nlm.nih.gov/2017/11/30/ncbi-southern-california-genomics-hackathon-january/" target="_blank">SoCal Bioinformatics Hackathon</a>. From January 9-11, 2019, the&nbsp;<a href="https://www.ncbi.nlm.nih.gov/" target="_blank">NCBI</a>&nbsp;will help run a bioinformatics hackathon in Southern California hosted by the&nbsp;<a href="http://www.csrc.sdsu.edu/" target="_blank">Computational Sciences Research Center</a>&nbsp;at&nbsp;<a href="http://www.sdsu.edu/" target="_blank">San Diego State University</a>!</p><p><span>NCBI Hackathon</span>&nbsp;specifically looking for folks who have experience in computational virus hunting or adjacent fields to identify known, taxonomically-definable and novel viruses from a few hundred thousand metagenomic datasets that we&rsquo;ll put on cloud infrastructure. This event is for researchers, including students and postdocs, who are already engaged in the use of bioinformatics data or in the development of pipelines for virological analyses from high-throughput experiments. If this describes you, please&nbsp;<a href="https://goo.gl/forms/kDnSG0IAZD62XQRe2" target="_blank">apply</a>! The event is open to anyone selected for the hackathon and willing to travel to SDSU (see below).</p><p>https://ncbiinsights.ncbi.nlm.nih.gov/2018/11/09/ncbi-sdsu-virus-hunting-data-science-hackathon-january-2019/</p>]]></description>
	<dc:creator>BioStar</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40573/de-novo-genome-assembly-for-illumina-data</guid>
	<pubDate>Mon, 20 Jan 2020 05:13:29 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40573/de-novo-genome-assembly-for-illumina-data</link>
	<title><![CDATA[De novo Genome Assembly for Illumina Data]]></title>
	<description><![CDATA[<p>Written and maintained by <a href="mailto:simon.gladman@unimelb.edu.au">Simon Gladman</a> - Melbourne Bioinformatics (formerly VLSCI)</p>
<p>Protocol Overview / Introduction</p>
<p>In this protocol we discuss and outline the process of de novo assembly for small to medium sized genomes.</p>
<p>https://www.melbournebioinformatics.org.au/tutorials/tutorials/assembly/assembly-protocol/</p><p>Address of the bookmark: <a href="https://www.melbournebioinformatics.org.au/tutorials/tutorials/assembly/assembly-protocol/" rel="nofollow">https://www.melbournebioinformatics.org.au/tutorials/tutorials/assembly/assembly-protocol/</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/42419/biojupies-automatically-generates-rna-seq-data-analysis-notebooks</guid>
	<pubDate>Sun, 20 Dec 2020 11:43:45 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/42419/biojupies-automatically-generates-rna-seq-data-analysis-notebooks</link>
	<title><![CDATA[BioJupies: Automatically Generates RNA-seq Data Analysis Notebooks]]></title>
	<description><![CDATA[<p>With BioJupies you can produce in seconds a customized, reusable, and interactive report from your own raw or processed RNA-seq data through a simple user interface</p>
<p>BioJupies now supports user accounts! Sign in from the top right corner of the page for access to unlimited private notebooks, RNA-seq datasets and alignment jobs.</p><p>Address of the bookmark: <a href="https://amp.pharm.mssm.edu/biojupies/" rel="nofollow">https://amp.pharm.mssm.edu/biojupies/</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44557/fundamentals-of-data-visualization-by-claus-o-wilke</guid>
	<pubDate>Sat, 08 Jun 2024 16:07:19 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44557/fundamentals-of-data-visualization-by-claus-o-wilke</link>
	<title><![CDATA[Fundamentals of Data Visualization by Claus O. Wilke]]></title>
	<description><![CDATA[<p><span><span>The book is meant as a guide to making visualizations that accurately reflect the data, tell a story, and look professional. It has grown out of my experience of working with students and postdocs in my laboratory on thousands of data visualizations. Over the years, I have noticed that the same issues arise over and over. I have attempted to collect my accumulated knowledge from these interactions in the form of this book.</span></span></p>
<p><span>The entire book is written in R Markdown, using RStudio as my text editor and the&nbsp;</span><span>bookdown</span><span>&nbsp;package to turn a collection of markdown documents into a coherent whole. The book&rsquo;s source code is hosted on GitHub, at&nbsp;</span><a href="https://github.com/clauswilke/dataviz">https://github.com/clauswilke/dataviz</a><span>.&nbsp;</span></p><p>Address of the bookmark: <a href="https://clauswilke.com/dataviz/" rel="nofollow">https://clauswilke.com/dataviz/</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/4590/tigers-genome-sequenced</guid>
	<pubDate>Tue, 17 Sep 2013 16:48:24 -0500</pubDate>
	<link>https://bioinformaticsonline.com/news/view/4590/tigers-genome-sequenced</link>
	<title><![CDATA[Tigers genome sequenced]]></title>
	<description><![CDATA[<p>Fifteen scientists led by Dr Jong Bhak of Genome Research Foundation, South Korea, decoded as many as 3 billion nucleotides (organic molecules that form the basic building blocks of nucleic acids, such as DNA). They identified 20,000 genes related to various functions of the tiger.&nbsp;</p><p>The biggest and perhaps most fearsome of the world's big cats, the tiger, shares 95.6 percent of its DNA with humans' cute and furry companions, domestic cats.</p><p>The new research showed that big cats have genetic mutations that enabled them to be carnivores. The team also identified mutations that allow snow leopards to thrive at high altitudes.</p><p>Reference:</p><p><a href="http://www.nbcnews.com/science/your-cat-ferocious-tigers-share-lot-95-6-percent-their-4B11182690">http://www.nbcnews.com/science/your-cat-ferocious-tigers-share-lot-95-6-percent-their-4B11182690</a></p><p><a href="http://timesofindia.indiatimes.com/home/environment/flora-fauna/Gene-mapping-of-tiger-completed/articleshow/22671681.cms">http://timesofindia.indiatimes.com/home/environment/flora-fauna/Gene-mapping-of-tiger-completed/articleshow/22671681.cms</a></p><p>Paper:</p><p><a href="http://www.nature.com/ncomms/2013/130917/ncomms3433/full/ncomms3433.html">http://www.nature.com/ncomms/2013/130917/ncomms3433/full/ncomms3433.html</a></p>]]></description>
	<dc:creator>Rahul Agarwal</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/36512/hisat2-a-fast-and-sensitive-alignment-program-for-mapping-next-generation-sequencing-reads</guid>
	<pubDate>Tue, 08 May 2018 04:27:22 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/36512/hisat2-a-fast-and-sensitive-alignment-program-for-mapping-next-generation-sequencing-reads</link>
	<title><![CDATA[HISAT2: a fast and sensitive alignment program for mapping next-generation sequencing reads]]></title>
	<description><![CDATA[<p><strong>HISAT2</strong><span>&nbsp;is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes (as well as to a single reference genome). Based on an extension of BWT for graphs&nbsp;</span><a href="http://dl.acm.org/citation.cfm?id=2674828">[Sir&eacute;n et al. 2014]</a><span>, we designed and implemented a graph FM index (GFM), an original approach and its first implementation to the best of our knowledge. In addition to using one global GFM index that represents a population of human genomes, HISAT2 uses a large set of small GFM indexes that collectively cover the whole genome (each index representing a genomic region of 56 Kbp, with 55,000 indexes needed to cover the human population). These small indexes (called local indexes), combined with several alignment strategies, enable rapid and accurate alignment of sequencing reads. This new indexing scheme is called a Hierarchical Graph FM index (HGFM).&nbsp;</span></p>
<p><span>more at&nbsp;https://ccb.jhu.edu/software/hisat2/index.shtml</span></p><p>Address of the bookmark: <a href="https://github.com/infphilo/hisat2" rel="nofollow">https://github.com/infphilo/hisat2</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37800/heatmapper-web-enabled-heat-mapping-for-all</guid>
	<pubDate>Mon, 01 Oct 2018 08:34:41 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37800/heatmapper-web-enabled-heat-mapping-for-all</link>
	<title><![CDATA[Heatmapper: web-enabled heat mapping for all]]></title>
	<description><![CDATA[<p><span>Heatmapper is a freely available web server that allows users to interactively visualize their data in the form of heat maps through an easy-to-use graphical interface. Heatmapper is a versatile tool that allows users to easily create a wide variety of heat maps for many different data types and applications. Heatmapper allows users to generate, cluster and visualize: </span></p>
<p><span>1)&nbsp;</span><span>expression-based heat maps</span><span>&nbsp;from transcriptomic, proteomic and metabolomic experiments; 2)&nbsp;</span><span>pairwise distance maps</span><span>; </span></p>
<p><span>3)&nbsp;</span><span>correlation maps</span><span>; </span></p>
<p><span>4)&nbsp;</span><span>image overlay heat maps</span><span>; </span></p>
<p><span>5)&nbsp;</span><span>latitude and longitude heat maps</span><span>&nbsp;and </span></p>
<p><span>6)&nbsp;</span><span>geopolitical (choropleth) heat maps</span><span>. </span></p>
<p><span>Heatmapper offers a number of simple and intuitive customization options for easy adjustments to each heat map&rsquo;s appearance and plotting parameters. Heatmapper also allows users to interactively explore their numeric data values by hovering their cursor over each heat map, or by using a searchable/sortable data table view.</span></p>
<p><span>Ref&nbsp;https://www.ncbi.nlm.nih.gov/pubmed/27190236</span></p><p>Address of the bookmark: <a href="http://www2.heatmapper.ca/" rel="nofollow">http://www2.heatmapper.ca/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/videolist/watch/11249/how-to-sequence-the-human-genome-mark-j-kiel</guid>
	<pubDate>Fri, 30 May 2014 13:24:11 -0500</pubDate>
	<link>https://bioinformaticsonline.com/videolist/watch/11249/how-to-sequence-the-human-genome-mark-j-kiel</link>
	<title><![CDATA[How to sequence the human genome - Mark J. Kiel]]></title>
	<description><![CDATA[<iframe width="" height="" src="https://www.youtube-nocookie.com/embed/MvuYATh7Y74" frameborder="0" allowfullscreen></iframe>View full lesson: http://ed.ted.com/lessons/how-to-sequence-the-human-genome-mark-j-kiel

Your genome, every human's genome, consists of a unique DNA sequence of A's, T's, C's and G's that tell your cells how to operate. Thanks to technological advances, scientists are now able to know the sequence of letters that makes up an individual genome relatively quickly and inexpensively. Mark J. Kiel takes an in-depth look at the science behind the sequence.

Lesson by Mark J. Kiel, animation by Marc Christoforidis.]]></description>
	
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27430/mosaik-a-hash-based-algorithm-for-accurate-next-generation-sequencing-short-read-mapping</guid>
	<pubDate>Fri, 20 May 2016 18:53:49 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27430/mosaik-a-hash-based-algorithm-for-accurate-next-generation-sequencing-short-read-mapping</link>
	<title><![CDATA[MOSAIK: A Hash-Based Algorithm for Accurate Next-Generation Sequencing Short-Read Mapping]]></title>
	<description><![CDATA[<p><span>MOSAIK is a stable, sensitive and open-source program for mapping second and third-generation sequencing reads to a reference genome. Uniquely among current mapping tools, MOSAIK can align reads generated by all the major sequencing technologies, including Illumina, Applied Biosystems SOLiD, Roche 454, Ion Torrent and Pacific BioSciences SMRT. Indeed, MOSAIK was the only aligner to provide consistent mappings for all the generated data (sequencing technologies, low-coverage and exome) in the 1000 Genomes Project. To provide highly accurate alignments, MOSAIK employs a hash clustering strategy coupled with the Smith-Waterman algorithm. This method is well-suited to capture mismatches as well as short insertions and deletions. To support the growing interest in larger structural variant (SV) discovery, MOSAIK provides explicit support for handling known-sequence SVs, e.g. mobile element insertions (MEIs) as well as generating outputs tailored to aid in SV discovery.</span></p><p>Address of the bookmark: <a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090581" rel="nofollow">http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090581</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
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