BOL: Related items

BUSCO datasets !

Jit — Wed, 13 Jan 2021 19:44:33 -0600

The BUSCO software now directly downloads the necessary datasets, specified by the user or automatically selected.

To display all available datasets

busco --list-datasets

You can also download them manually and see a list with the number of marker genes.

Earlier versions: v3, v2, v1

../
acidobacteria_odb10.2020-03-06.tar.gz              16-Oct-2020 13:19     14M
aconoidasida_odb10.2020-08-05.tar.gz               16-Oct-2020 13:19     51M
actinobacteria_class_odb10.2020-03-06.tar.gz       16-Oct-2020 13:19      8M
actinobacteria_phylum_odb10.2020-03-06.tar.gz      16-Oct-2020 13:20      9M
actinopterygii_odb10.2020-08-05.tar.gz             16-Oct-2020 13:20    173M
agaricales_odb10.2020-08-05.tar.gz                 16-Oct-2020 13:20    186M
agaricomycetes_odb10.2020-08-05.tar.gz             16-Oct-2020 13:19    152M
alphaproteobacteria_odb10.2020-03-06.tar.gz        16-Oct-2020 13:20     11M
alteromonadales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:20     18M
alveolata_odb10.2020-09-10.tar.gz                  16-Oct-2020 13:20     13M
apicomplexa_odb10.2020-09-10.tar.gz                16-Oct-2020 13:19     27M
aquificae_odb10.2020-03-06.tar.gz                  16-Oct-2020 13:19     12M
arachnida_odb10.2020-08-05.tar.gz                  16-Oct-2020 13:19    136M
archaea_odb10.2020-03-06.tar.gz                    16-Oct-2020 13:20      4M
arthropoda_odb10.2020-09-10.tar.gz                 16-Oct-2020 13:20     95M
ascomycota_odb10.2020-09-10.tar.gz                 16-Oct-2020 13:20    295M
aves_odb10.2020-09-10.tar.gz                       16-Oct-2020 13:20    465M
bacillales_odb10.2020-03-06.tar.gz                 16-Oct-2020 13:20     10M
bacilli_odb10.2020-03-06.tar.gz                    16-Oct-2020 13:19      7M
bacteria_odb10.2020-03-06.tar.gz                   16-Oct-2020 13:20      6M
bacteroidales_odb10.2020-03-06.tar.gz              16-Oct-2020 13:20     13M
bacteroidetes-chlorobi_group_odb10.2020-03-06.t..> 16-Oct-2020 13:19      9M
bacteroidetes_odb10.2020-03-06.tar.gz              16-Oct-2020 13:20     10M
bacteroidia_odb10.2020-03-06.tar.gz                16-Oct-2020 13:20     13M
basidiomycota_odb10.2020-09-10.tar.gz              16-Oct-2020 13:19    165M
betaproteobacteria_odb10.2020-03-06.tar.gz         16-Oct-2020 13:19     13M
boletales_odb10.2020-08-05.tar.gz                  16-Oct-2020 13:19    197M
brassicales_odb10.2020-08-05.tar.gz                16-Oct-2020 13:20    158M
burkholderiales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     16M
campylobacterales_odb10.2020-03-06.tar.gz          16-Oct-2020 13:20     14M
capnodiales_odb10.2020-08-05.tar.gz                16-Oct-2020 13:19    178M
carnivora_odb10.2020-08-05.tar.gz                  16-Oct-2020 13:19    376M
cellvibrionales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     19M
cetartiodactyla_odb10.2020-08-05.tar.gz            16-Oct-2020 13:19    407M
chaetothyriales_odb10.2020-08-05.tar.gz            16-Oct-2020 13:20    288M
chlamydiae_odb10.2020-03-06.tar.gz                 16-Oct-2020 13:20     13M
chlorobi_odb10.2020-03-06.tar.gz                   16-Oct-2020 13:20     22M
chloroflexi_odb10.2020-03-06.tar.gz                16-Oct-2020 13:20      6M
chlorophyta_odb10.2020-08-05.tar.gz                16-Oct-2020 13:20     58M
chromatiales_odb10.2020-03-06.tar.gz               16-Oct-2020 13:19     14M
chroococcales_odb10.2020-03-06.tar.gz              16-Oct-2020 13:19     27M
clostridia_odb10.2020-03-06.tar.gz                 16-Oct-2020 13:19      6M
clostridiales_odb10.2020-03-06.tar.gz              16-Oct-2020 13:20      6M
coccidia_odb10.2020-08-05.tar.gz                   16-Oct-2020 13:20     24M
coriobacteriales_odb10.2020-03-06.tar.gz           16-Oct-2020 13:19     12M
coriobacteriia_odb10.2020-03-06.tar.gz             16-Oct-2020 13:20      9M
corynebacteriales_odb10.2020-03-06.tar.gz          16-Oct-2020 13:19     16M
cyanobacteria_odb10.2020-03-06.tar.gz              16-Oct-2020 13:19     17M
cyprinodontiformes_odb10.2020-08-05.tar.gz         16-Oct-2020 13:19    504M
cytophagales_odb10.2020-03-06.tar.gz               16-Oct-2020 13:19     18M
cytophagia_odb10.2020-03-06.tar.gz                 16-Oct-2020 13:20     18M
delta-epsilon-subdivisions_odb10.2020-03-06.tar.gz 16-Oct-2020 13:19      6M
deltaproteobacteria_odb10.2020-03-06.tar.gz        16-Oct-2020 13:19      6M
desulfobacterales_odb10.2020-03-06.tar.gz          16-Oct-2020 13:20     13M
desulfovibrionales_odb10.2020-03-06.tar.gz         16-Oct-2020 13:19     17M
desulfurococcales_odb10.2020-03-06.tar.gz          16-Oct-2020 13:20      9M
desulfuromonadales_odb10.2020-03-06.tar.gz         16-Oct-2020 13:19     18M
diptera_odb10.2020-08-05.tar.gz                    16-Oct-2020 13:19    185M
dothideomycetes_odb10.2020-08-05.tar.gz            16-Oct-2020 13:19    194M
embryophyta_odb10.2020-09-10.tar.gz                16-Oct-2020 13:19    138M
endopterygota_odb10.2020-09-10.tar.gz              16-Oct-2020 13:19    190M
enterobacterales_odb10.2020-03-06.tar.gz           16-Oct-2020 13:19     11M
entomoplasmatales_odb10.2020-03-06.tar.gz          16-Oct-2020 13:19      7M
epsilonproteobacteria_odb10.2020-03-06.tar.gz      16-Oct-2020 13:20     13M
euarchontoglires_odb10.2020-09-10.tar.gz           16-Oct-2020 13:20    728M
eudicots_odb10.2020-09-10.tar.gz                   16-Oct-2020 13:19    168M
euglenozoa_odb10.2020-08-05.tar.gz                 16-Oct-2020 13:20     10M
eukaryota_odb10.2020-09-10.tar.gz                  16-Oct-2020 13:19    100M
eurotiales_odb10.2020-08-05.tar.gz                 16-Oct-2020 13:19    211M
eurotiomycetes_odb10.2020-08-05.tar.gz             16-Oct-2020 13:19    197M
euryarchaeota_odb10.2020-03-06.tar.gz              16-Oct-2020 13:20      5M
eutheria_odb10.2020-09-10.tar.gz                   16-Oct-2020 13:20    920M
fabales_odb10.2020-08-05.tar.gz                    16-Oct-2020 13:19    184M
firmicutes_odb10.2020-03-06.tar.gz                 16-Oct-2020 13:20      6M
flavobacteriales_odb10.2020-03-06.tar.gz           16-Oct-2020 13:19     18M
flavobacteriia_odb10.2020-03-06.tar.gz             16-Oct-2020 13:20     18M
fungi_odb10.2020-09-10.tar.gz                      16-Oct-2020 13:19    181M
fusobacteria_odb10.2020-03-06.tar.gz               16-Oct-2020 13:19     11M
fusobacteriales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     11M
gammaproteobacteria_odb10.2020-03-06.tar.gz        16-Oct-2020 13:19     10M
glires_odb10.2020-08-05.tar.gz                     16-Oct-2020 13:19    549M
glomerellales_odb10.2020-08-05.tar.gz              16-Oct-2020 13:19    259M
halobacteria_odb10.2020-03-06.tar.gz               16-Oct-2020 13:19     18M
halobacteriales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:20     18M
haloferacales_odb10.2020-03-06.tar.gz              16-Oct-2020 13:19     20M
helotiales_odb10.2020-08-05.tar.gz                 16-Oct-2020 13:19    224M
hemiptera_odb10.2020-08-05.tar.gz                  16-Oct-2020 13:19    115M
hymenoptera_odb10.2020-08-05.tar.gz                16-Oct-2020 13:19    321M
hypocreales_odb10.2020-08-05.tar.gz                16-Oct-2020 13:19    224M
insecta_odb10.2020-09-10.tar.gz                    16-Oct-2020 13:20    119M
lactobacillales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19      9M
laurasiatheria_odb10.2020-09-10.tar.gz             16-Oct-2020 13:20    688M
legionellales_odb10.2020-03-06.tar.gz              16-Oct-2020 13:19     16M
leotiomycetes_odb10.2020-08-05.tar.gz              16-Oct-2020 13:19    164M
lepidoptera_odb10.2020-08-05.tar.gz                16-Oct-2020 13:20    241M
liliopsida_odb10.2020-09-10.tar.gz                 16-Oct-2020 13:20    163M
mammalia_odb10.2020-09-10.tar.gz                   16-Oct-2020 13:19    677M
metazoa_odb10.2020-09-10.tar.gz                    16-Oct-2020 13:19    127M
methanobacteria_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     15M
methanococcales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:20     17M
methanomicrobia_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     12M
methanomicrobiales_odb10.2020-03-06.tar.gz         16-Oct-2020 13:20     16M
micrococcales_odb10.2020-03-06.tar.gz              16-Oct-2020 13:19     12M
microsporidia_odb10.2020-08-05.tar.gz              16-Oct-2020 13:20     26M
mollicutes_odb10.2020-03-06.tar.gz                 16-Oct-2020 13:20      3M
mollusca_odb10.2020-08-05.tar.gz                   16-Oct-2020 13:20    266M
mucorales_odb10.2020-08-05.tar.gz                  16-Oct-2020 13:20    105M
mucoromycota_odb10.2020-08-05.tar.gz               16-Oct-2020 13:19     75M
mycoplasmatales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19      4M
natrialbales_odb10.2020-03-06.tar.gz               16-Oct-2020 13:19     24M
neisseriales_odb10.2020-03-06.tar.gz               16-Oct-2020 13:19     16M
nematoda_odb10.2020-08-05.tar.gz                   16-Oct-2020 13:20    138M
nitrosomonadales_odb10.2020-03-06.tar.gz           16-Oct-2020 13:19     15M
nostocales_odb10.2020-03-06.tar.gz                 16-Oct-2020 13:19     35M
oceanospirillales_odb10.2020-03-06.tar.gz          16-Oct-2020 13:19     13M
onygenales_odb10.2020-08-05.tar.gz                 16-Oct-2020 13:19    233M
oscillatoriales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     29M
passeriformes_odb10.2020-08-05.tar.gz              16-Oct-2020 13:20    298M
pasteurellales_odb10.2020-03-06.tar.gz             16-Oct-2020 13:19     19M
planctomycetes_odb10.2020-03-06.tar.gz             16-Oct-2020 13:19     13M
plasmodium_odb10.2020-08-05.tar.gz                 16-Oct-2020 13:20    199M
pleosporales_odb10.2020-08-05.tar.gz               16-Oct-2020 13:19    288M
poales_odb10.2020-08-05.tar.gz                     16-Oct-2020 13:19    186M
polyporales_odb10.2020-08-05.tar.gz                16-Oct-2020 13:20    206M
primates_odb10.2020-08-05.tar.gz                   16-Oct-2020 13:19    451M
propionibacteriales_odb10.2020-03-06.tar.gz        16-Oct-2020 13:19     14M
proteobacteria_odb10.2020-03-06.tar.gz             16-Oct-2020 13:19      8M
pseudomonadales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     17M
rhizobiales_odb10.2020-03-06.tar.gz                16-Oct-2020 13:19     15M
rhizobium-agrobacterium_group_odb10.2020-03-06...> 16-Oct-2020 13:19     34M
rhodobacterales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     19M
rhodospirillales_odb10.2020-03-06.tar.gz           16-Oct-2020 13:19     14M
rickettsiales_odb10.2020-03-06.tar.gz              16-Oct-2020 13:19      8M
saccharomycetes_odb10.2020-08-05.tar.gz            16-Oct-2020 13:20    109M
sauropsida_odb10.2020-09-10.tar.gz                 16-Oct-2020 13:19    499M
selenomonadales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     16M
solanales_odb10.2020-08-05.tar.gz                  16-Oct-2020 13:19    196M
sordariomycetes_odb10.2020-08-05.tar.gz            16-Oct-2020 13:19    206M
sphingobacteriia_odb10.2020-03-06.tar.gz           16-Oct-2020 13:20     21M
sphingomonadales_odb10.2020-03-06.tar.gz           16-Oct-2020 13:19     22M
spirochaetales_odb10.2020-03-06.tar.gz             16-Oct-2020 13:20      8M
spirochaetes_odb10.2020-03-06.tar.gz               16-Oct-2020 13:20      6M
spirochaetia_odb10.2020-03-06.tar.gz               16-Oct-2020 13:19      6M
stramenopiles_odb10.2020-08-05.tar.gz              16-Oct-2020 13:19      5M
streptomycetales_odb10.2020-03-06.tar.gz           16-Oct-2020 13:20     31M
streptosporangiales_odb10.2020-03-06.tar.gz        16-Oct-2020 13:19     22M
sulfolobales_odb10.2020-03-06.tar.gz               16-Oct-2020 13:19     22M
synechococcales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     16M
synergistetes_odb10.2020-03-06.tar.gz              16-Oct-2020 13:19     14M
tenericutes_odb10.2020-03-06.tar.gz                16-Oct-2020 13:20      4M
tetrapoda_odb10.2020-09-10.tar.gz                  16-Oct-2020 13:19    561M
thaumarchaeota_odb10.2020-03-06.tar.gz             16-Oct-2020 13:20     13M
thermoanaerobacterales_odb10.2020-03-06.tar.gz     16-Oct-2020 13:19     10M
thermoplasmata_odb10.2020-03-06.tar.gz             16-Oct-2020 13:20      7M
thermoproteales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19      8M
thermoprotei_odb10.2020-03-06.tar.gz               16-Oct-2020 13:20      6M
thermotogae_odb10.2020-03-06.tar.gz                16-Oct-2020 13:19     16M
thiotrichales_odb10.2020-03-06.tar.gz              16-Oct-2020 13:20     11M
tissierellales_odb10.2020-03-06.tar.gz             16-Oct-2020 13:19      9M
tissierellia_odb10.2020-03-06.tar.gz               16-Oct-2020 13:19      8M
tremellomycetes_odb10.2020-08-05.tar.gz            16-Oct-2020 13:19    210M
verrucomicrobia_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     11M
vertebrata_odb10.2020-09-10.tar.gz                 16-Oct-2020 13:19    409M
vibrionales_odb10.2020-03-06.tar.gz                16-Oct-2020 13:20     26M
viridiplantae_odb10.2020-09-10.tar.gz              16-Oct-2020 13:20     36M
xanthomonadales_odb10.2020-03-06.tar.gz            16-Oct-2020 13:19     24M

Address of the bookmark: https://busco-data.ezlab.org/v4/data/lineages/

ncbi-datasets-cli -- Quickstart: command line tools !

Jit — Tue, 07 Dec 2021 02:51:26 -0600

Install and use the NCBI Datasets command line tools

The NCBI Datasets datasets command line tools are datasets and dataformat .

Use datasets to download biological sequence data across all domains of life from NCBI.

Use dataformat to convert metadata from JSON Lines format to other formats.

Conda download:

https://anaconda.org/conda-forge/ncbi-datasets-cli

Buld Download

https://www.ncbi.nlm.nih.gov/datasets/builder/?tax_id=29979

Address of the bookmark: https://www.ncbi.nlm.nih.gov/datasets/docs/v1/quickstarts/command-line-tools/

dbCAN: a web server and DataBase for automated Carbohydrate-active enzyme ANnotation

Jit — Mon, 29 May 2017 05:39:29 -0500

dbCAN is a web server and DataBase for automated Carbohydrate-active enzyme ANnotation, funded by the BioEnergy Science Center of the DOE. Similar resources on the web include CAZy database and CAT. All data in dbCAN are generated based on the family classification from CAZy database while it has the following unique features compared with CAZy database and CAT:

dbCAN provides the capability of automated and comprehensive CAZyme annotation of a given genome submitted by the user;
dbCAN provides an explicitly defined signature domain for each and every CAZyme family along with its location in all the relevant full-length CAZyme proteins in all sequenced genomes;
dbCAN provides the most complete set of metagenomic CAZyme genes published so far and represents the first step towards discovering novel CAZyme catalysts in metagenomes;
dbCAN provides a subfamily classification of the existing CAZyme families based on sequence similarities;
dbCAN make all pre-computed data freely available to the public, including sequence alignments, hidden markov models (HMMs) and phylogenies of the signature domain regions in each and every CAZyme family and subfamily.

dbCAN is updated regularly when CAZy database created new families based on latest literature.

Address of the bookmark: http://csbl.bmb.uga.edu/dbCAN/index.php

Phased Human Genome Assembly !

Rahul Nayak — Mon, 08 Oct 2018 09:10:54 -0500

The new publicly available assembly (PacBio HG00733) has the fewest gaps of any human genome assembly, with more than half of the genome contained in gapless sequence at least 27 Mb long. The primary contig assembly is 2.89 Gb long and consists of 865 contigs that were assembled with PacBio data generated with the company’s Sequel® System. Using the FALCON-Unzip assembler, maternal and paternal haplotypes were resolved over more than 80% of the genome. Maternal and paternal haplotype blocks were then further phased using Hi-C technology and the FALCON-Phase methoddeveloped in collaboration with Phase Genomics. The genome was then de novo scaffolded using Phase Genomics’ Proximo Hi-C platform, resulting in the first chromosome-scale diploid assembly of a single individual accomplished with only two technologies. More specific details about the assembly are included on the PacBio blog.

The data are available using NCBI accession IDs: BioProject: (PRJNA483067), assembly: [RBJD00000000] and sequence data (SRP155659).

Additional Resources

Interactive map showcasing global initiatives underway to generate reference-quality human genome assemblies for diverse populations
BioReport Podcast on the value of ethnic-specific reference genomes
Nature Reviews Genetics paper from NHGRI: Prioritizing diversity in human genomics research
Article in The Journal of Precision Medicine: “Minority Report – Ethnic Diversity and the Real Promise for Precision Medicine”
Article in Bio-IT World: “Genomic Data Standards Are a Necessity”
NHGRI Project Award: High Quality Human and Non-Human Primate Genome Assemblies

More details are available on the PacBio website:

Blog post: Data Release: Highest-Quality, Most Contiguous Individual Human Genome Assembly to Date
Blog post: For Reference-Grade Human Genome Assemblies, SMRT Sequencing Yields Optimal Results
Webinar: Assembling High-Quality Human Reference Genomes for Global Populations
FALCON-Phase press release and article preprint
PacBio research focus webpage about Human Population Genetics

Ref: https://stockguru.com/2018/10/08/pacific-biosciences-releases-highest-quality-most-contiguous-individual-human-genome-assembly-to-date/

Entire Human Genome Sequencing !

LEGE — Tue, 02 Apr 2024 01:19:29 -0500

Cost-effective whole human genome sequencing has revolutionized the landscape of genetic research and personalized medicine by making comprehensive genetic analysis accessible to a wider population. Through advancements in sequencing technologies, such as next-generation sequencing (NGS), costs have significantly decreased, enabling researchers and healthcare providers to analyze an individual's complete genetic makeup with greater efficiency and affordability. This has profound implications for disease diagnosis, prognosis, and treatment, as it allows for the identification of genetic predispositions and the customization of healthcare interventions based on an individual's unique genetic profile. Moreover, as the cost continues to decline, the potential for population-scale genomic studies and large-scale screening programs becomes increasingly feasible, promising to further enhance our understanding of human genetics and improve healthcare outcomes on a global scale.

Here are few companies:

https://mynucleus.com/

https://myome.com/

https://nebula.org/whole-genome-sequencing-dna-test/

Carefully opt for human reference genome

biogeek — Tue, 18 Feb 2020 07:43:32 -0600

Heng Li posted several issues with the human reference genomes given in these resources and suggests the following compressed FASTA file to be used as hg38/GRCh38 human reference genome.

if you map reads to GRCh38 or hg38, use the following:

ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/000/001/405/GCA_000001405.15_GRCh38/seqs_for_alignment_pipelines.ucsc_ids/GCA_000001405.15_GRCh38_no_alt_analysis_set.fna.gz

There are several other versions of GRCh37/GRCh38. What’s wrong with them? Here are a collection of potential issues:

More at http://lh3.github.io/2017/11/13/which-human-reference-genome-to-use

Address of the bookmark: http://lh3.github.io/2017/11/13/which-human-reference-genome-to-use

The complete sequence of a human genome

Neel — Thu, 31 Mar 2022 23:58:18 -0500

The completed regions include all centromeric satellite arrays, recent segmental duplications, and the short arms of all five acrocentric chromosomes, unlocking these complex regions of the genome to variational and functional studies.

Address of the bookmark: https://www.science.org/doi/10.1126/science.abj6987