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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/39370?offset=380</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44585/dram-distilled-and-refined-annotation-of-metabolism</guid>
	<pubDate>Sat, 06 Jul 2024 04:19:45 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44585/dram-distilled-and-refined-annotation-of-metabolism</link>
	<title><![CDATA[DRAM: Distilled and Refined Annotation of Metabolism]]></title>
	<description><![CDATA[<p><span>DRAM (Distilled and Refined Annotation of Metabolism) is a tool for annotating metagenomic assembled genomes and&nbsp;</span><a href="https://github.com/simroux/VirSorter">VirSorter</a><span>&nbsp;identified viral contigs. DRAM annotates MAGs and viral contigs using&nbsp;</span><a href="https://www.kegg.jp/">KEGG</a><span>&nbsp;(if provided by the user),&nbsp;</span><a href="https://www.uniprot.org/">UniRef90</a><span>,&nbsp;</span><a href="https://pfam.xfam.org/">PFAM</a><span>,&nbsp;</span><a href="http://bcb.unl.edu/dbCAN2/">dbCAN</a><span>,&nbsp;</span><a href="https://www.ncbi.nlm.nih.gov/genome/viruses/">RefSeq viral</a><span>,&nbsp;</span><a href="http://vogdb.org/">VOGDB</a><span>&nbsp;and the&nbsp;</span><a href="https://www.ebi.ac.uk/merops/">MEROPS</a><span>&nbsp;peptidase database as well as custom user databases. DRAM is run in two stages. First an annotation step to assign database identifiers to gene, and then a distill step to curate these annotations into useful functional categories. Additionally, viral contigs are further analyzed during to identify potential AMGs. This is done via assigning an auxiliary score and flags representing the confidence that a gene is both metabolic and viral.</span></p>
<p><img src="https://genomicsaotearoa.github.io/metagenomics_summer_school/figures/ex14_DRAM_annotation_rank.png" alt="image" style="border: 0px;"></p>
<p>Ref&nbsp;https://genomicsaotearoa.github.io/metagenomics_summer_school/day4/ex15_gene_annotation_part3/#overview-of-drampy-annotate-output&nbsp;</p><p>Address of the bookmark: <a href="https://github.com/WrightonLabCSU/DRAM" rel="nofollow">https://github.com/WrightonLabCSU/DRAM</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31156/splitbam-splits-a-bam-by-chromosomes</guid>
	<pubDate>Tue, 28 Feb 2017 09:01:28 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31156/splitbam-splits-a-bam-by-chromosomes</link>
	<title><![CDATA[splitbam: splits a BAM by chromosomes]]></title>
	<description><![CDATA[<p><strong>splitbam</strong>&nbsp;splits a BAM by chromosomes.</p>
<p>Using the reference sequence dictionary (<code>*.dict</code>), it also creates some empty BAM files if no sam record was found for a chromosome. A pair of 'mock' SAM-Records can also be added to those empty BAMs to avoid some tools (like samtools) to crash.</p>
<h1>Usage</h1>
<p><code>java -jar splitbam.jar -p OUT/__CHROM__/__CHROM__.bam -R ref.fasta (bam|sam|stdin)</code></p>
<h1>Options</h1>
<ul>
<li>-h help; This screen.</li>
<li>-R (indexed reference file) REQUIRED.</li>
<li>-u (unmapped chromosome name): default:Unmapped</li>
<li>-e | --empty : generate EMPTY bams for chromosome having no read mapped</li>
<li>-m | --mock : if option '-e', add a mock pair of sam records to the empty bam</li>
<li>-p (output file/bam pattern) REQUIRED. MUST contain&nbsp;<strong><code>__CHROM__</code></strong>&nbsp;and end with .bam</li>
<li>-s assume input is sorted.</li>
<li>-x | --index create index.</li>
<li>-t | --tmp (dir) tmp file directory</li>
<li>-G (file) chrom-group file (see below)</li>
</ul><p>Address of the bookmark: <a href="https://code.google.com/archive/p/jvarkit/wikis/SplitBam.wiki" rel="nofollow">https://code.google.com/archive/p/jvarkit/wikis/SplitBam.wiki</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31300/clgenomics</guid>
	<pubDate>Fri, 03 Mar 2017 09:57:28 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31300/clgenomics</link>
	<title><![CDATA[CLgenomics]]></title>
	<description><![CDATA[<p>CLgenomics is a standalone desktop software specifically designed for bacterial genome analysis. This program has a powerful multi-genome browser, which enables rapid and responsive exploration of bacterial genomes.</p>
<p>To use CLgenomics, individual genome data (genome sequences + annotation details) are compiled and saved in a specially formatted file called CLG (ChunLab Genomics).&nbsp;Each CLG file corresponds with one bacterial genome. If multiple genomes are being considered and compared, multiple CLG files are needed. ChunLab offers &gt;40,000 CLG files of publicly available Bacterial and Archaeal genomes.</p><p>Address of the bookmark: <a href="https://chunlab.wordpress.com/clgenomics-software/" rel="nofollow">https://chunlab.wordpress.com/clgenomics-software/</a></p>]]></description>
	<dc:creator>Radha Agarkar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38659/detail-annotation-of-genes</guid>
	<pubDate>Fri, 11 Jan 2019 05:23:33 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38659/detail-annotation-of-genes</link>
	<title><![CDATA[Detail annotation of genes !]]></title>
	<description><![CDATA[<p>gene_info recalculated daily<br>---------------------------------------------------------------------------<br> tab-delimited<br> one line per GeneID<br> Column header line is the first line in the file.<br> Note: subsets of gene_info are available in the DATA/GENE_INFO<br> directory (described later)<br>---------------------------------------------------------------------------</p>
<p>tax_id:<br> the unique identifier provided by NCBI Taxonomy<br> for the species or strain/isolate</p>
<p>GeneID:<br> the unique identifier for a gene<br> ASN1: geneid</p>
<p>Symbol:<br> the default symbol for the gene<br> ASN1: gene-&gt;locus</p>
<p>LocusTag:<br> the LocusTag value<br> ASN1: gene-&gt;locus-tag</p>
<p>Synonyms:<br> bar-delimited set of unofficial symbols for the gene</p>
<p>dbXrefs:<br> bar-delimited set of identifiers in other databases<br> for this gene. The unit of the set is database:value.<br> Note that HGNC and MGI include 'HGNC' and 'MGI', respectively,<br> in the value part of their identifier. Consequently,<br> dbXrefs for these databases will appear like:<br> HGNC:HGNC:1100<br> This would be interpreted as database='HGNC', value='HGNC:1100'<br> Example for MGI:<br> MGI:MGI:104537<br> This would be interpreted as database='MGI', value='MGI:104537'</p>
<p>chromosome:<br> the chromosome on which this gene is placed.<br> for mitochondrial genomes, the value 'MT' is used.</p>
<p>map location:<br> the map location for this gene</p>
<p>description:<br> a descriptive name for this gene</p>
<p>type of gene:<br> the type assigned to the gene according to the list of options<br> provided in https://www.ncbi.nlm.nih.gov/IEB/ToolBox/CPP_DOC/lxr/source/src/objects/entrezgene/entrezgene.asn</p>
<p><br>Symbol from nomenclature authority:<br> when not '-', indicates that this symbol is from a<br> a nomenclature authority</p>
<p>Full name from nomenclature authority:<br> when not '-', indicates that this full name is from a<br> a nomenclature authority</p>
<p>Nomenclature status:<br> when not '-', indicates the status of the name from the <br> nomenclature authority (O for official, I for interim)</p>
<p>Other designations:<br> pipe-delimited set of some alternate descriptions that<br> have been assigned to a GeneID<br> '-' indicates none is being reported.</p>
<p>Modification date:<br> the last date a gene record was updated, in YYYYMMDD format</p>
<p>Feature type:<br> pipe-delimited set of annotated features and their classes or <br> controlled vocabularies, displayed as feature_type:feature_class <br> or feature_type:controlled_vocabulary, when appropriate; derived <br> from select feature annotations on RefSeq(s) associated with the <br> GeneID</p><p>Address of the bookmark: <a href="ftp://ftp.ncbi.nih.gov/gene/DATA/GENE_INFO/" rel="nofollow">ftp://ftp.ncbi.nih.gov/gene/DATA/GENE_INFO/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31353/concoct-clustering-contigs-with-coverage-and-composition</guid>
	<pubDate>Mon, 06 Mar 2017 04:08:16 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31353/concoct-clustering-contigs-with-coverage-and-composition</link>
	<title><![CDATA[CONCOCT: Clustering cONtigs with COverage and ComposiTion]]></title>
	<description><![CDATA[<p>A program for unsupervised binning of metagenomic contigs by using nucleotide composition, coverage data in multiple samples and linkage data from paired end reads.</p>
<p>Warning! This software is to be considered under development. Functionality and the user interface may still change significantly from one version to another. If you want to use this software, please stay up to date with the list of known issues:<a href="https://github.com/BinPro/CONCOCT/issues">https://github.com/BinPro/CONCOCT/issues</a></p><p>Address of the bookmark: <a href="https://github.com/BinPro/CONCOCT" rel="nofollow">https://github.com/BinPro/CONCOCT</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40598/mitoz-a-toolkit-for-animal-mitochondrial-genome-assembly-annotation-and-visualization</guid>
	<pubDate>Fri, 24 Jan 2020 04:09:15 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40598/mitoz-a-toolkit-for-animal-mitochondrial-genome-assembly-annotation-and-visualization</link>
	<title><![CDATA[MitoZ: a toolkit for animal mitochondrial genome assembly, annotation and visualization]]></title>
	<description><![CDATA[<p><span>MitoZ is a Python3-based toolkit which aims to automatically filter pair-end raw data (fastq files), assemble genome, search for mitogenome sequences from the genome assembly result, annotate mitogenome (genbank file as result), and mitogenome visualization. MitoZ is available from&nbsp;</span><code>https://github.com/linzhi2013/MitoZ</code><span>.</span></p>
<p><span><a href="https://academic.oup.com/nar/article/47/11/e63/5377471">https://academic.oup.com/nar/article/47/11/e63/5377471</a></span></p><p>Address of the bookmark: <a href="https://github.com/linzhi2013/MitoZ" rel="nofollow">https://github.com/linzhi2013/MitoZ</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31382/seqmule-automated-human-exomegenome-variants-detection</guid>
	<pubDate>Tue, 07 Mar 2017 10:12:36 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31382/seqmule-automated-human-exomegenome-variants-detection</link>
	<title><![CDATA[SeqMule: Automated human exome/genome variants detection]]></title>
	<description><![CDATA[<p><span>SeqMule takes single-end or paird-end FASTQ or BAM files, generates a script consisting of more than 10 popular alignment, analysis tools and runs the script line by line. Users can change the pipeline or fine-tune the parameters by modifying its configuration file. SeqMule also has some built-in functions, such as pooling consensus calls from various callers, plotting a Venn diagram showing intersection among different callers, and downloading databases. SeqMule can be used for both Mendelian disease study and cancer genome study.</span></p><p>Address of the bookmark: <a href="http://seqmule.openbioinformatics.org/en/latest/" rel="nofollow">http://seqmule.openbioinformatics.org/en/latest/</a></p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/42491/eukulele-taxonomic-annotation-of-the-unsung-eukaryotic-microbes</guid>
	<pubDate>Sat, 26 Dec 2020 12:10:17 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/42491/eukulele-taxonomic-annotation-of-the-unsung-eukaryotic-microbes</link>
	<title><![CDATA[EUKulele: Taxonomic annotation of the unsung eukaryotic microbes]]></title>
	<description><![CDATA[<p><span><span>&nbsp;</span>EUKulele, an open-source software tool designed to assign taxonomy to microeukaryotes detected in meta-omic samples, and complement analysis approaches in other domains by accommodating assembly output and providing concrete metrics reporting the taxonomic completeness of each sample.</span></p><p>Address of the bookmark: <a href="https://github.com/AlexanderLabWHOI/EUKulele" rel="nofollow">https://github.com/AlexanderLabWHOI/EUKulele</a></p>]]></description>
	<dc:creator>Shruti Paniwala</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31564/htslib</guid>
	<pubDate>Wed, 15 Mar 2017 11:38:05 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31564/htslib</link>
	<title><![CDATA[HTSlib]]></title>
	<description><![CDATA[<p>Samtools is a suite of programs for interacting with high-throughput sequencing data. It consists of three separate repositories:</p>
<dl><dt>Samtools</dt><dd>Reading/writing/editing/indexing/viewing SAM/BAM/CRAM format</dd><dt>BCFtools</dt><dd>Reading/writing BCF2/VCF/gVCF files and calling/filtering/summarising SNP and short indel sequence variants</dd><dt>HTSlib</dt><dd>A C library for reading/writing high-throughput sequencing data</dd></dl>
<p>Samtools and BCFtools both use HTSlib internally, but these source packages contain their own copies of htslib so they can be built independently.</p><p>Address of the bookmark: <a href="http://www.htslib.org/" rel="nofollow">http://www.htslib.org/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43614/mitoz-a-toolkit-for-animal-mitochondrial-genome-assembly-annotation-and-visualization</guid>
	<pubDate>Tue, 30 Nov 2021 23:23:57 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43614/mitoz-a-toolkit-for-animal-mitochondrial-genome-assembly-annotation-and-visualization</link>
	<title><![CDATA[MitoZ: a toolkit for animal mitochondrial genome assembly, annotation and visualization]]></title>
	<description><![CDATA[<p>MitoZ, consisting of independent modules of <em>de novo</em> assembly, findMitoScaf (find Mitochondrial Scaffolds), annotation and visualization, that can generate mitogenome assembly together with annotation and visualization results from HTS raw reads.</p>
<p>https://academic.oup.com/nar/article/47/11/e63/5377471</p><p>Address of the bookmark: <a href="https://github.com/linzhi2013/MitoZ" rel="nofollow">https://github.com/linzhi2013/MitoZ</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
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