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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/40208?offset=180</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32190/dbg2olcefficient-assembly-of-large-genomes-using-long-erroneous-reads-of-the-third-generation-sequencing-technologies</guid>
	<pubDate>Wed, 19 Apr 2017 10:09:51 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32190/dbg2olcefficient-assembly-of-large-genomes-using-long-erroneous-reads-of-the-third-generation-sequencing-technologies</link>
	<title><![CDATA[DBG2OLC:Efficient Assembly of Large Genomes Using Long Erroneous Reads of the Third Generation Sequencing Technologies]]></title>
	<description><![CDATA[<p>DBG2OLC:Efficient Assembly of Large Genomes Using Long Erroneous Reads of the Third Generation Sequencing Technologies</p>
<p>Our work is published in Scientific Reports:</p>
<p>Ye, C. et al. DBG2OLC: Efficient Assembly of Large Genomes Using Long Erroneous Reads of the Third Generation Sequencing Technologies. Sci. Rep. 6, 31900; doi: 10.1038/srep31900 (2016).</p>
<p><a href="http://www.nature.com/articles/srep31900">http://www.nature.com/articles/srep31900</a></p>
<p>The manual can be downloaded from:</p>
<p><a href="https://github.com/yechengxi/DBG2OLC/raw/master/Manual.docx">https://github.com/yechengxi/DBG2OLC/raw/master/Manual.docx</a></p>
<p>To use precompiled versions,please go to:</p>
<p><a href="https://github.com/yechengxi/DBG2OLC/tree/master/compiled">https://github.com/yechengxi/DBG2OLC/tree/master/compiled</a></p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="https://github.com/yechengxi/DBG2OLC" rel="nofollow">https://github.com/yechengxi/DBG2OLC</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32948/simba-a-web-tool-for-managing-bacterial-genome-assembly-generated-by-ion-pgm-sequencing-technology</guid>
	<pubDate>Tue, 23 May 2017 05:28:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32948/simba-a-web-tool-for-managing-bacterial-genome-assembly-generated-by-ion-pgm-sequencing-technology</link>
	<title><![CDATA[SIMBA: a web tool for managing bacterial genome assembly generated by Ion PGM sequencing technology]]></title>
	<description><![CDATA[<p><span>SIMBA</span><span>, SImple Manager for Bacterial Assemblies, is a Web interface for managing assembly projects of bacterial genomes. SIMBA was created to assist bioinformaticians to assemble bacterial genomes sequenced with NextGeneration Sequencing (NGS) platforms quickly, easily and effectively. SIMBA also is open source tool, i.e., can be freely downloaded, shared and modified.</span></p>
<p>https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-016-1344-7</p><p>Address of the bookmark: <a href="http://ufmg-simba.sourceforge.net/" rel="nofollow">http://ufmg-simba.sourceforge.net/</a></p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/36837/ranbow-a-haplotype-assembler-for-polyploid-genomes</guid>
	<pubDate>Fri, 01 Jun 2018 07:21:54 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/36837/ranbow-a-haplotype-assembler-for-polyploid-genomes</link>
	<title><![CDATA[Ranbow: a haplotype assembler for polyploid genomes]]></title>
	<description><![CDATA[Ranbow is a haplotype assembler for polyploid genomes. It has been developed for the haplotype assembly of the hexaploid sweet potato genome, which is highly heterozygous. Ranbow can also be applied to other polyploid genomes. After a first phasing, Ranbow utilizes the assembled haplotypes to improve the accuracy of variant calling results and to infer the evolutionary history of the organism´s genome. Ranbow has three main modes of function:

ranbow hap: for haplotyping
ranbow eval: for evaluating of the assemble haplotypes by gold standard (long) reads 
ranbow phylo: for the phylogenetic analysis<p>Address of the bookmark: <a href="https://www.molgen.mpg.de/ranbow" rel="nofollow">https://www.molgen.mpg.de/ranbow</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41691/genobuntu-package-for-next-generation-sequencing-and-genome-assembly</guid>
	<pubDate>Mon, 18 May 2020 16:47:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41691/genobuntu-package-for-next-generation-sequencing-and-genome-assembly</link>
	<title><![CDATA[Genobuntu: Package for Next Generation Sequencing and Genome Assembly]]></title>
	<description><![CDATA[<div>
<p>Genobuntu is a software package containing more than 70 software and packages oriented towards NGS. In its current version, Genobuntu supports pre assembly tools, genome assemblers as well as post assembly tools.<br><br>Commonly used biological software and example script files for different assembly pipelines have also been provided, where the example script files can be updated to suit one&rsquo;s experimental needs. Genobuntu attempts to reduce the amount of time and energy needed to build software workstations and it can also act as a good teaching source for a class room setting.<br><br>Therefore, Genobuntu offers a well-tailored environment for both novices and experts working in the field of genome assembly.</p>
</div>
<div>
<h3>Features</h3>
<ul>
<li>Velvet</li>
<li>MiB</li>
<li>SSAKE</li>
<li>EULER</li>
<li>VCAKE</li>
<li>ABySS</li>
<li>ALLPATHS</li>
<li>Celera</li>
<li>SHARCGS</li>
<li>Allpaths</li>
<li>IDBA</li>
<li>TAIPAN</li>
<li>Edena</li>
<li>SOAPdenovo</li>
<li>Maq</li>
<li>IDBA-UD</li>
<li>No. of Reads present in the Ref. Seq.</li>
<li>ART NGS Reads Simulator</li>
<li>HiTEC, FASTQC</li>
<li>Minimum Description Length</li>
<li>SOAPaligner</li>
<li>Sequencing Read Archive Toolkit</li>
</ul>
</div><p>Address of the bookmark: <a href="https://sourceforge.net/projects/genobuntu/" rel="nofollow">https://sourceforge.net/projects/genobuntu/</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43110/quasimodo-quasispecies-metric-determination-on-omics</guid>
	<pubDate>Sat, 26 Jun 2021 15:22:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43110/quasimodo-quasispecies-metric-determination-on-omics</link>
	<title><![CDATA[QuasiModo - Quasispecies Metric Determination on Omics]]></title>
	<description><![CDATA[<p><span>This repository contains the scripts and pipeline that reproduces the results of the HCMV benchmarking study. In this study we evaluated genome assemblers and variant callers on 10 in vitro generated, mixed strain HCMV sequence samples, each consisting of two lab strains in different abundance ratios. This tool can also be used to evaluate assemblies and variant calling results on other similar datasets.</span></p>
<p><span>https://academic.oup.com/bib/article/22/3/bbaa123/5868070</span></p><p>Address of the bookmark: <a href="https://github.com/hzi-bifo/Quasimodo" rel="nofollow">https://github.com/hzi-bifo/Quasimodo</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44489/proksee</guid>
	<pubDate>Wed, 27 Mar 2024 11:11:54 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44489/proksee</link>
	<title><![CDATA[Proksee]]></title>
	<description><![CDATA[<p><span>Proksee is an expert system for genome assembly, annotation and visualization. To begin using Proksee, provide a complete genome sequence, sequencing reads or a CGView/Proksee map JSON file.</span></p>
<fieldset><legend>Please Cite the Following</legend>
<div>Grant JR, Enns E, Marinier E, Mandal A, Herman EK, Chen C, Graham M, Van Domselaar G, and Stothard P</div>
<div><a href="https://pubmed.ncbi.nlm.nih.gov/37140037/">Proksee: in-depth characterization and visualization of bacterial genomes</a></div>
<div>Nucleic Acids Research, 2023, gkad326, https://doi.org/10.1093/nar/gkad326</div>
</fieldset><p>Address of the bookmark: <a href="https://proksee.ca/" rel="nofollow">https://proksee.ca/</a></p>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27845/cnidaria-fast-reference-free-phylogenomic-clustering</guid>
	<pubDate>Thu, 16 Jun 2016 17:55:17 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27845/cnidaria-fast-reference-free-phylogenomic-clustering</link>
	<title><![CDATA[CNIDARIA: fast, reference-free phylogenomic clustering]]></title>
	<description><![CDATA[<p>Motivation: Identification of biological specimens is a major requirement for a range of applications. Reference-free methods analyse unprocessed sequencing data without relying on prior knowledge, but these do not scale to arbitrarily large genomes and arbitrarily large phylogenetic distances.</p>
<p>Results: We present Cnidaria, a practical tool for clustering genomic and transcriptomic data with no limitation on ge-nome size or phylogenetic distances. We successfully simultaneously clustered 169 genomic and transcriptomic datasets from 4 kingdoms, achieving 100% accuracy at supra-species level and 78% accuracy for species level.</p>
<p>Availability and Implementation: Cnidaria is written in C++ and Python and is available at http://www.ab.wur.nl/cnidaria.</p>
<p>Contact: Saulo Aflitos - sauloal@gmail.com</p>
<p>Supplementary information: Supplementary data are available at Bioinformatics online.</p><p>Address of the bookmark: <a href="https://github.com/sauloal/cnidaria/wiki" rel="nofollow">https://github.com/sauloal/cnidaria/wiki</a></p>]]></description>
	<dc:creator>Shruti Paniwala</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34493/plast-a-fast-accurate-and-ngs-scalable-bank-to-bank-sequence-similarity-search-tool</guid>
	<pubDate>Fri, 01 Dec 2017 04:10:54 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34493/plast-a-fast-accurate-and-ngs-scalable-bank-to-bank-sequence-similarity-search-tool</link>
	<title><![CDATA[PLAST: A fast, accurate and NGS scalable bank-to-bank sequence similarity search tool]]></title>
	<description><![CDATA[<p><strong>PLAST is a fast, accurate and NGS scalable bank-to-bank sequence similarity search tool providing significant accelerations of seeds-based heuristic comparison methods, such as the Blast suite of algorithms.</strong></p>
<p><strong>Relying on unique software architecture, PLAST takes full advantage of recent multi-core personal computers without requiring any additional hardware devices.</strong></p>
<p>PLAST stands for&nbsp;<em>Parallel Local Sequence Alignment Search Tool&nbsp;</em>and is was&nbsp;<a href="http://www.biomedcentral.com/1471-2105/10/329" target="_blank">published in BMC Bioinformatics.</a></p>
<p>PLAST is a general purpose sequence comparison tool providing the following benefits:</p>
<ul>
<li>PLAST is a high-performance sequence comparison tool designed to compare two sets of sequences (query vs. reference),</li>
<li>Reduces the processing time of sequences comparisons while providing highest quality results,</li>
<li>Contains a fully integrated data filtering engine capable of selecting relevant hits with user-defined criteria (E-Value, identity, coverage, alignment length, etc.),</li>
<li>Does not require any additional hardware, since it is a software solution. It is easy to install, cost-effective, takes full advantage of multi-core processors and uses a small RAM footprint,</li>
<li>Ready to be used on desktop computer, cluster, cloud as well as within distributed system running Hadoop.</li>
</ul>
<p>https://plast.inria.fr/</p><p>Address of the bookmark: <a href="https://plast.inria.fr/" rel="nofollow">https://plast.inria.fr/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/35883/arcs-scaffolding-genome-drafts-with-linked-reads</guid>
	<pubDate>Tue, 06 Mar 2018 16:35:26 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/35883/arcs-scaffolding-genome-drafts-with-linked-reads</link>
	<title><![CDATA[ARCS: scaffolding genome drafts with linked reads]]></title>
	<description><![CDATA[<p><span>ARCS, an application that utilizes the barcoding information contained in linked reads to further organize draft genomes into highly contiguous assemblies. We show how the contiguity of an ABySS&nbsp;</span><em>H.sapiens</em><span>genome assembly can be increased over six-fold, using moderate coverage (25-fold) Chromium data. We expect ARCS to have broad utility in harnessing the barcoding information contained in linked read data for connecting high-quality sequences in genome assembly drafts.</span></p><p>Address of the bookmark: <a href="https://github.com/bcgsc/ARCS/" rel="nofollow">https://github.com/bcgsc/ARCS/</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/37411/my-commonly-used-commands-in-bioinformatics</guid>
	<pubDate>Thu, 26 Jul 2018 04:58:45 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/37411/my-commonly-used-commands-in-bioinformatics</link>
	<title><![CDATA[My commonly used commands in Bioinformatics]]></title>
	<description><![CDATA[<p>FYI, I've found it useful to use MUMmer to extract the specific changes that Racon makes, so I can evaluate them individually:</p><pre><code>minimap -t 24 assembly.fasta long_reads.fastq.gz | racon -t 24 long_reads.fastq.gz - assembly.fasta racon_assembly.fasta
nucmer -p nucmer assembly.fasta racon_assembly.fasta
show-snps -C -T -r nucmer.delta
</code></pre><p>This reports Racon's changes in a table. You can exclude indels with the&nbsp;<code>-I</code>&nbsp;option in&nbsp;<code>show-snps</code>.&nbsp;</p><p>This process (Racon -&gt; MUMmer -&gt; SNP table) solves the problem I originally raised in this issue. So as far as I'm concerned, you can close this issue (or keep it open if you still want to implement some kind of variant table).</p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>

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