<![CDATA[BOL: Related items]]> https://bioinformaticsonline.com/related/41405?offset=190 https://bioinformaticsonline.com/bookmarks/view/41602/nucdiff-in-depth-characterization-and-annotation-of-differences-between-two-sets-of-dna-sequences Tue, 05 May 2020 10:35:48 -0500 https://bioinformaticsonline.com/bookmarks/view/41602/nucdiff-in-depth-characterization-and-annotation-of-differences-between-two-sets-of-dna-sequences <![CDATA[NucDiff: In-depth characterization and annotation of differences between two sets of DNA sequences]]> NucDiff locates and categorizes differences between two closely related nucleotide sequences. It is able to deal with very fragmented genomes, structural rearrangements and various local differences. These features make NucDiff to be perfectly suitable to compare assemblies with each other or with available reference genomes.

NucDiff provides information about the types of differences and their locations. It is possible to upload the results into genome browser for visualization and further inspection. It was written in Python and uses the NUCmer package from MUMmer[1] for sequence comparison.



Address of the bookmark: https://github.com/uio-cels/NucDiff

]]> Jit https://bioinformaticsonline.com/blog/view/44227/common-methods-to-discover-tandem-repeats Thu, 09 Mar 2023 02:40:52 -0600 https://bioinformaticsonline.com/blog/view/44227/common-methods-to-discover-tandem-repeats <![CDATA[Common methods to discover tandem repeats]]>

Tandem repeats are DNA sequences that are repeated in a contiguous manner in the genome. These sequences are often used as genetic markers and are important in many areas of genetics and genomics research. Here are some methods for discovering tandem repeats in genomes:

  1. Tandem Repeat Finder: Tandem Repeat Finder is a software tool that identifies tandem repeats in DNA sequences. It is available for free download and can be used on both nucleotide and protein sequences. The tool uses a statistical algorithm to identify repeats based on their length, copy number, and overall composition.

  2. RepeatMasker: RepeatMasker is another software tool that can identify tandem repeats in DNA sequences. It works by comparing the input sequence to a database of known repeats and then identifies any tandem repeats that match those in the database.

  3. PCR-based methods: Polymerase chain reaction (PCR) can be used to amplify and detect tandem repeats in genomic DNA. PCR primers are designed to flank the tandem repeat region, and amplification of the target DNA fragment can be visualized on a gel. This method can be useful for detecting novel tandem repeats and for genotyping.

  4. Southern blotting: Southern blotting is a classic method for detecting DNA fragments in a sample. It can be used to detect tandem repeats by digesting genomic DNA with a restriction enzyme, separating the fragments by gel electrophoresis, and then probing the blot with a tandem repeat-specific probe.

Overall, a combination of these methods can be used to comprehensively identify tandem repeats in genomes.

]]> BioStar https://bioinformaticsonline.com/bookmarks/view/44878/jaeger-an-accurate-and-fast-deep-learning-tool-to-detect-bacteriophage-sequences Thu, 14 Aug 2025 04:02:05 -0500 https://bioinformaticsonline.com/bookmarks/view/44878/jaeger-an-accurate-and-fast-deep-learning-tool-to-detect-bacteriophage-sequences <![CDATA[Jaeger : an accurate and fast deep-learning tool to detect bacteriophage sequences]]> Jaeger is a tool that utilizes homology-free machine learning to identify phage genome sequences that are hidden within metagenomes. It is capable of detecting both phages and prophages within metagenomic assemblies.

The performance of the Jaeger workflow can be significantly increased by utilizing GPUs. To enable GPU support, the CUDA Toolkit and cuDNN library must be accessible to conda.

# setup bioconda
conda config --add channels defaults
conda config --add channels bioconda
conda config --add channels conda-forge
conda config --set channel_priority strict

# create conda environment and install jaeger
mamba create -n jaeger -c nvidia -c conda-forge cuda-nvcc "python>=3.9,<3.12" pip jaeger-bio


# activate environment
conda activate jaeger

Address of the bookmark: https://github.com/MGXlab/Jaeger

]]> Neel