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<channel>
	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/42166?offset=340</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37984/baum-%E2%80%93-improving-genome-assembly-by-adaptive-unique-mapping-and-local-overlap-layout-consensus</guid>
	<pubDate>Wed, 24 Oct 2018 23:35:09 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37984/baum-%E2%80%93-improving-genome-assembly-by-adaptive-unique-mapping-and-local-overlap-layout-consensus</link>
	<title><![CDATA[BAUM – Improving Genome Assembly by Adaptive Unique Mapping and Local Overlap-Layout-Consensus]]></title>
	<description><![CDATA[<p><span>BAUM, breaks the whole genome into regions by adaptive unique mapping; then the local OLC is used to assemble each region in parallel. BAUM can: (1) perform reference-assisted assembly based on the genome of a close species; (2) or improve the results of existing assemblies that are obtained based on short or long sequencing reads.&nbsp;</span></p><p>Address of the bookmark: <a href="http://www.zhanyuwang.xin/wordpress/index.php/2017/07/21/baum-improving-genome-assembly-by-adaptive-unique-mapping-and-local-overlap-layout-consensus/" rel="nofollow">http://www.zhanyuwang.xin/wordpress/index.php/2017/07/21/baum-improving-genome-assembly-by-adaptive-unique-mapping-and-local-overlap-layout-consensus/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40208/ragoo-fast-reference-guided-scaffolding-of-genome-assembly-contigs</guid>
	<pubDate>Sun, 27 Oct 2019 00:57:23 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40208/ragoo-fast-reference-guided-scaffolding-of-genome-assembly-contigs</link>
	<title><![CDATA[RaGOO: Fast Reference-Guided Scaffolding of Genome Assembly Contigs]]></title>
	<description><![CDATA[<p>Alonge M, Soyk S, Ramakrishnan S, Wang X, Goodwin S, Sedlazeck FJ, Lippman ZB, Schatz MC:&nbsp;<a href="https://www.biorxiv.org/content/early/2019/01/13/519637">Fast and accurate reference-guided scaffolding of draft genomes</a>.&nbsp;<em>bioRxiv</em>&nbsp;2019.</p>
<p>RaGOO is a tool for coalescing genome assembly contigs into pseudochromosomes via minimap2 alignments to a closely related reference genome. The focus of this tool is on practicality and therefore has the following features:</p>
<ol>
<li>Good performance. On a MacBook Pro using Arabidopsis data, pseudochromosome construction takes less than a minute and the whole pipeline with SV calling takes ~2 minutes.</li>
<li>Intact ordering and orienting of contigs.</li>
<li><a href="https://github.com/malonge/RaGOO/wiki/Misassembly-Correction">Misassembly correction</a></li>
<li><a href="https://github.com/malonge/RaGOO/wiki/GFF-File-Lift-Over">GFF lift-over</a></li>
<li><a href="https://github.com/malonge/RaGOO/wiki/Calling-Structural-Variants">Structural variant calling with and integrated version of Assemblytics</a></li>
<li>Confidence scores associated with the grouping, localization, and orientation for each contig.</li>
</ol><p>Address of the bookmark: <a href="https://github.com/malonge/RaGOO" rel="nofollow">https://github.com/malonge/RaGOO</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41691/genobuntu-package-for-next-generation-sequencing-and-genome-assembly</guid>
	<pubDate>Mon, 18 May 2020 16:47:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41691/genobuntu-package-for-next-generation-sequencing-and-genome-assembly</link>
	<title><![CDATA[Genobuntu: Package for Next Generation Sequencing and Genome Assembly]]></title>
	<description><![CDATA[<div>
<p>Genobuntu is a software package containing more than 70 software and packages oriented towards NGS. In its current version, Genobuntu supports pre assembly tools, genome assemblers as well as post assembly tools.<br><br>Commonly used biological software and example script files for different assembly pipelines have also been provided, where the example script files can be updated to suit one&rsquo;s experimental needs. Genobuntu attempts to reduce the amount of time and energy needed to build software workstations and it can also act as a good teaching source for a class room setting.<br><br>Therefore, Genobuntu offers a well-tailored environment for both novices and experts working in the field of genome assembly.</p>
</div>
<div>
<h3>Features</h3>
<ul>
<li>Velvet</li>
<li>MiB</li>
<li>SSAKE</li>
<li>EULER</li>
<li>VCAKE</li>
<li>ABySS</li>
<li>ALLPATHS</li>
<li>Celera</li>
<li>SHARCGS</li>
<li>Allpaths</li>
<li>IDBA</li>
<li>TAIPAN</li>
<li>Edena</li>
<li>SOAPdenovo</li>
<li>Maq</li>
<li>IDBA-UD</li>
<li>No. of Reads present in the Ref. Seq.</li>
<li>ART NGS Reads Simulator</li>
<li>HiTEC, FASTQC</li>
<li>Minimum Description Length</li>
<li>SOAPaligner</li>
<li>Sequencing Read Archive Toolkit</li>
</ul>
</div><p>Address of the bookmark: <a href="https://sourceforge.net/projects/genobuntu/" rel="nofollow">https://sourceforge.net/projects/genobuntu/</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43110/quasimodo-quasispecies-metric-determination-on-omics</guid>
	<pubDate>Sat, 26 Jun 2021 15:22:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43110/quasimodo-quasispecies-metric-determination-on-omics</link>
	<title><![CDATA[QuasiModo - Quasispecies Metric Determination on Omics]]></title>
	<description><![CDATA[<p><span>This repository contains the scripts and pipeline that reproduces the results of the HCMV benchmarking study. In this study we evaluated genome assemblers and variant callers on 10 in vitro generated, mixed strain HCMV sequence samples, each consisting of two lab strains in different abundance ratios. This tool can also be used to evaluate assemblies and variant calling results on other similar datasets.</span></p>
<p><span>https://academic.oup.com/bib/article/22/3/bbaa123/5868070</span></p><p>Address of the bookmark: <a href="https://github.com/hzi-bifo/Quasimodo" rel="nofollow">https://github.com/hzi-bifo/Quasimodo</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44223/ale-assembly-likelihood-estimator</guid>
	<pubDate>Wed, 08 Mar 2023 01:39:33 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44223/ale-assembly-likelihood-estimator</link>
	<title><![CDATA[ALE: Assembly Likelihood Estimator]]></title>
	<description><![CDATA[<p>Just import the assembly, bam and ALE scores. You can convert the .ale file to a set of .wig files with ale2wiggle.py and IGV can read those directly.&nbsp; Depending on your genome size you may want to convert the .wig files to the BigWig format.</p><p>Address of the bookmark: <a href="https://github.com/sc932/ALE" rel="nofollow">https://github.com/sc932/ALE</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/opportunity/view/4004/33rd-annual-convention-of-indian-association-for-cancer-research-from-13th-to-15th-february-2014</guid>
  <pubDate>Tue, 27 Aug 2013 10:37:08 -0500</pubDate>
  <link></link>
  <title><![CDATA[33rd Annual Convention of Indian Association for Cancer Research from 13th to 15th February 2014]]></title>
  <description><![CDATA[
<p>RGCB is organizing the 33rd Annual Convention of Indian Association for Cancer Research from 13th to 15th February 2014 with the theme "Discovery, Innovation and Translation in Cancer Research"</p>

<p>Kindly log on to conference website http://rgcb.res.in/IACR2014 for further details and timely updates and registration. We shall truly appreciate if the same be circulated among your friends, scholars and students encouraging them to participate in the meet.</p>

<p>http://210.212.237.38/iacrconference/</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/44002/interesting-bioinformatics-resources</guid>
	<pubDate>Fri, 11 Nov 2022 06:30:46 -0600</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/44002/interesting-bioinformatics-resources</link>
	<title><![CDATA[Interesting Bioinformatics Resources !]]></title>
	<description><![CDATA[<p>1. a reproducible workflow.&nbsp;<a href="https://www.youtube.com/watch?v=s3JldKoA0zw">https://www.youtube.com/watch?v=s3JldKoA0zw</a>&nbsp;This two minute video will change your mind on reproducible research&nbsp;</p><p>2. Parallel sequencing lives, or what makes large sequencing projects successful&nbsp;<a href="https://academic.oup.com/gigascience/article/6/11/gix100/4557140?login=false">https://academic.oup.com/gigascience/article/6/11/gix100/4557140?login=false</a></p><p>3. Common-sense approaches to sharing tabular data alongside publication&nbsp;<a href="https://www.sciencedirect.com/science/article/pii/S2666389921002300">https://www.sciencedirect.com/science/article/pii/S2666389921002300</a></p><p>4. A Reproducible Data Analysis Workflow with R Markdown, Git, Make, and Docker&nbsp;<a href="https://psyarxiv.com/8xzqy/">https://psyarxiv.com/8xzqy/</a></p><p>5. Practical Computational Reproducibility in the Life Sciences&nbsp;<a href="https://www.cell.com/cell-systems/fulltext/S2405-4712(18)30140-6">https://www.cell.com/cell-systems/fulltext/S2405-4712(18)30140-6</a></p><p>6. A video by Dr.Keith A. Baggerly from MD Anderson [The Importance of Reproducible Research in High-Throughput Biology](<a href="https://www.youtube.com/watch?v=7gYIs7uYbMo">https://www.youtube.com/watch?v=7gYIs7uYbMo</a>) highly recommended.</p><p>7. Ten Simple Rules for Reproducible Computational Research&nbsp;<a href="http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1003285">http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1003285</a>)</p><p>8. Good Enough Practices in Scientific Computing&nbsp;<a href="http://arxiv.org/abs/1609.00037">http://arxiv.org/abs/1609.00037</a>&nbsp;</p><p>9. Best Practices for Scientific Computing&nbsp;<a href="https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001745">https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001745</a></p><p>10. A Quick Guide to Organizing Computational Biology Projects&nbsp;<a href="http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.100042">http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.100042</a>&nbsp; A must read for computational biologists!</p><p>11. Reproducibility of computational workflows is automated using continuous analysis&nbsp;<a href="https://www.nature.com/articles/nbt.3780">https://www.nature.com/articles/nbt.3780</a></p><p>12. Five selfish reasons to work reproducibly&nbsp;<a href="https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0850-7">https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0850-7</a></p>]]></description>
	<dc:creator>Abhi</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/fun/view/4196/chemical-elements-of-bioinformatics</guid>
	<pubDate>Tue, 03 Sep 2013 16:35:39 -0500</pubDate>
	<link>https://bioinformaticsonline.com/fun/view/4196/chemical-elements-of-bioinformatics</link>
	<title><![CDATA[Chemical Elements of Bioinformatics]]></title>
	<description><![CDATA[<p>You must be familiar with periodic table and colour pattern, but this time you are going to amaze by new elements table by Eagle genomics. Just check it out and have fun :)</p><p><a href="http://elements.eaglegenomics.com/">http://elements.eaglegenomics.com/</a></p>]]></description>
	<dc:creator>Rahul Agarwal</dc:creator>
</item>

<item>
  <guid isPermaLink='true'>https://bioinformaticsonline.com/researchlabs/view/40881/liu-lab</guid>
  <pubDate>Tue, 04 Feb 2020 06:27:02 -0600</pubDate>
  <link></link>
  <title><![CDATA[Liu Lab]]></title>
  <description><![CDATA[
<p>Shirley is a computational biologist with expertise in cancer epigenetics. Her research focuses on algorithm development and integrative mining from big data generated on microarrays, massively parallel sequencing, and other high throughput techniques to model the specificity and function of transcription factors, chromatin regulators and lncRNAs in tumor development, progression, drug response and resistance.</p>

<p>https://liulab-dfci.github.io/software/</p>
]]></description>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/42275/frequent-parameters-for-bioinformatics-tools</guid>
	<pubDate>Tue, 27 Oct 2020 19:42:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/42275/frequent-parameters-for-bioinformatics-tools</link>
	<title><![CDATA[Frequent parameters for bioinformatics tools !]]></title>
	<description><![CDATA[<div><div>Third party executable parameters and options.</div><div>&nbsp;</div><div>Trimmomatic</div><div>&nbsp;</div><div>&ldquo;ILLUMINACLIP:...:2:30:10&rdquo;</div><div>&ldquo;LEADING:15&rdquo;</div><div>&ldquo;TRAILING:15&rdquo;</div><div>&ldquo;SLIDINGWINDOW:4:20&rdquo;</div><div>&ldquo;MINLEN:20&rdquo;</div><div>&ldquo;TOPHRED33&rdquo;</div><div>&nbsp;</div><div>Filtlong</div><div>--min_length 500</div><div>--min_mean_q 85</div><div>--min_window_q 65</div><div>&nbsp;</div><div>FastQ Screen</div><div>--aligner bowtie2' (bwa for PacBio)</div><div>--subset 1000 (for PacBio)</div><div>&nbsp;</div><div>SPAdes</div><div>--careful</div><div>--disable-gzip-output</div><div>--cov-cutoff auto</div><div>--phred-offset 33</div><div>&nbsp;</div><div>HGAP</div><div>Pbalign.task_options.min_accuracy: 70</div><div>Pbalign.task_options.no_split_subreads: false</div><div>Genomic_consensus.task_options.min_confidence: 40</div><div>falcon_ns.task_options.HGAP_GenomeLength_str:</div><div>6000000</div><div>Pbcoretools.task_options.read_length: 0</div><div>Genomic_consensus.task_options.use_score: 0</div><div>Pbalign.task_options.min_length: 50</div><div>Pbalign.task_options.algorithm_options: --minMatch 12</div><div>--bestn 10 --minPctSimilarity 70.0</div><div>Pbalign.task_options.hit_policy: randombest</div><div>Pbcoretools.task_options.other_filters: rq &gt;= 0.7</div><div>Pbalign.task_options.concordant: false</div><div>Genomic_consensus.task_options.min_coverage: 5</div><div>falcon_ns.task_options.HGAP_SeedCoverage_str: 30</div><div>falcon_ns.task_options.HGAP_AggressiveAsm_bool: false</div><div>Genomic_consensus.task_options.algorithm: best</div><div>falcon_ns.task_options.HGAP_SeedLengthCutoff_str: -1</div><div>Genomic_consensus.task_options.diploid: false</div><div>&nbsp;</div><div>MeDuSa</div><div>-random 100</div><div>&nbsp;</div><div>Prokka</div><div>--usegenus</div><div>--force</div><div>--addgenes</div><div>--rfam</div><div>--rawproduct</div><div>&nbsp;</div><div>cmsearch (taxonomy, 16S)</div><div>--rfam</div><div>--noali</div><div>&nbsp;</div><div>blastn (taxonomy, 16S)</div><div>-evalue 1E-10</div><div>&nbsp;</div><div>blastn (MLST)</div><div>-ungapped</div></div><div><div>-dust no</div><div>-evalue 1E-20</div><div>-word_size 32</div><div>-culling_limit 2</div><div>-perc_identity 95</div><div>&nbsp;</div><div>blastp (VF)</div><div>-culling_limit 2</div><div>&nbsp;</div><div>RGI (ABR)</div><div>--input_type contig</div><div>&nbsp;</div><div>bowtie2 (mapping)</div><div>--sensitive</div><div>&nbsp;</div><div>minimap2 (mapping)</div><div>-a</div><div>-x map-ont</div><div>&nbsp;</div><div>samtools mpileup (SNP&nbsp;detection)</div><div>-uRI</div><div>&nbsp;</div><div>bcftools call (SNP detection)</div><div>--variants-only</div><div>--skip-variants indels</div><div>--output-type v</div><div>--ploidy 1</div><div>-c</div><div>&nbsp;</div><div>SNPsift filter (SNP detection)</div><div>"( QUAL &gt;= 30 ) &amp; (( na FILTER ) | (FILTER = 'PASS')) &amp;</div><div>( DP &gt;= 20 ) &amp; ( MQ &gt;= 20 )"</div><div>&nbsp;</div><div>SNPeff ann (SNP detection)</div><div>-nodownload</div><div>-no-intron</div><div>-no-downstream</div><div>-no SPLICE_SITE_REGION</div><div>-upDownStreamLen 250</div><div>&nbsp;</div><div>bcftools consensus</div><div>(phylogenetic tree)</div><div>--haplotype 1</div><div>&nbsp;</div><div>fasttreemp</div><div>-nt</div><div>-boot 100</div><div>&nbsp;</div><div>roary</div><div>-e</div><div>-n</div><div>-cd 100</div><div>-g 100000</div></div>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>

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