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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/43634?offset=250</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/3868/next-generation-sequencing-ngs-tutorials</guid>
	<pubDate>Sat, 24 Aug 2013 06:01:37 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/3868/next-generation-sequencing-ngs-tutorials</link>
	<title><![CDATA[Next Generation Sequencing (NGS) Tutorials]]></title>
	<description><![CDATA[<p>Institute of computational biomedicine, Cornell University provide an NGS workshop tutorial at&nbsp;<a href="http://chagall.med.cornell.edu/NGScourse/">http://chagall.med.cornell.edu/NGScourse/</a>&nbsp;</p>
<p>You can also add your favourite NGS educational material, or workshop tutorial by commenting on this bookmarks for user benefit.&nbsp;</p>
<p>Understanding the basics of genome sequencing:</p>
<p>Tutorial by Luke Jostins.</p>
<p>http://www.genetic-inference.co.uk/blog/2009/04/basics-sequencing-dna-part-1/</p>
<p>http://www.genetic-inference.co.uk/blog/2009/08/basics-sequencing-dna-part-2/</p>
<p>A window into third-generation sequencing</p>
<p>http://hmg.oxfordjournals.org/content/19/R2/R227.full.pdf</p>
<p>==============================================</p>
<p>NGS data analysis pipelines</p>
<ul>
<li><strong>Detecting and annotating genetic variations using the HugeSeq pipeline</strong>&nbsp; DOI: <a href="http://dx.doi.org/10.1038/nbt.2134">10.1038/nbt.2134</a></li>
<li><strong> NARWHAL, a primary analysis pipeline for NGS data</strong> <a href="http://bioinformatics.oxfordjournals.org/cgi/content/abstract/28/2/284?etoc">http://bioinformatics.oxfordjournals.org/cgi/content/abstract/28/2/284?etoc</a></li>
<li><strong>RseqFlow: Workflows for RNA-Seq data analysis</strong>&nbsp; DOI: <a href="http://dx.doi.org/10.1093/bioinformatics/btr441">10.1093/bioinformatics/btr441</a></li>
<li><strong>ngs_backbone: a pipeline for read cleaning, mapping and SNP calling using Next Generation Sequence</strong>&nbsp;&nbsp;<a href="http://dx.doi.org/10.1186/1471-2164-12-285">10.1186/1471-2164-12-285</a></li>
<li><strong>A framework for variation discovery and genotyping using next-generation DNA sequencing data</strong>&nbsp; PubMed: <a href="http://www.ncbi.nlm.nih.gov/pubmed/21478889">21478889</a></li>
<li><strong>SNiPlay: a web-based tool for detection, management and analysis of SNPs. Application to grapevine diversity projects</strong>&nbsp; DOI: <a href="http://dx.doi.org/10.1186/1471-2105-12-134">10.1186/1471-2105-12-134</a> Abstract: <a href="http://www.biomedcentral.com/1471-2105/12/134/abstract">http://www.biomedcentral.com/1471-2105/12/134/abstract</a></li>
<li><strong>WEP: a high-performance analysis pipeline for whole-exome data&nbsp;</strong>http://www.biomedcentral.com/1471-2105/14/S7/S11</li>
<li><strong>DDBJ read annotation pipeline: a cloud computing-based pipeline for high-throughput analysis of next-generation sequencing data.&nbsp;</strong>http://www.ncbi.nlm.nih.gov/pubmed/23657089</li>
<li><strong>GATK: a Toolkit for Genome Analysis&nbsp;</strong>http://www.broadinstitute.org/gatk/</li>
<li><strong>Metagenomics</strong>:http://www.nbic.nl/education/nbic-phd-school/course-schedule/ngsmetagenomics/</li>
<li><strong>RNASeq</strong>:http://www.nbic.nl/education/nbic-phd-school/course-schedule/ngsrnaseq/</li>
<li><strong>Bioinformatics and Seq courses</strong>:&nbsp;http://www.isb-sib.ch/training/training-activities-schedule/archive-2013.html</li>
<li><strong>Variant Detection (Model organism) Advanced tutorial</strong> https://docs.google.com/document/pub?id=1CuKkKylVDb03tnN7RSWl5EUzleetn0ctjmvaidPKLxM</li>
<li><strong>Variant Detection Introductory tutorial</strong> https://docs.google.com/document/pub?id=1ZRzrjjOCvtAu3m-IKL-rbJ1f4On60dDL_IEwG7oejdI</li>
<li><strong>Microbial de novo Assembly for Illumina Data Introductory tutorial</strong> https://docs.google.com/document/pub?id=1N3AB9ptISUu4zULqe1kXpVF0BDyGb5f5yzxWSJd_WNM</li>
<li><strong>RNAseq Differential Gene Expression Introductory tutorial</strong> https://docs.google.com/document/pub?id=1KbTiBHtvHLfPRZ39AY3uriazrINA8TJzgjjwn1zPP7Y</li>
</ul>
<blockquote>
<p>" Please add your favourite NGS link below in comment section for the benefit of bioinformatics community ".&nbsp;</p>
</blockquote><p>Address of the bookmark: <a href="http://chagall.med.cornell.edu/NGScourse/" rel="nofollow">http://chagall.med.cornell.edu/NGScourse/</a></p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34394/tulip-the-uncorrected-long-read-itegration-pipeline</guid>
	<pubDate>Thu, 23 Nov 2017 09:30:01 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34394/tulip-the-uncorrected-long-read-itegration-pipeline</link>
	<title><![CDATA[TULIP - The Uncorrected Long read Itegration Pipeline]]></title>
	<description><![CDATA[<p>#Running TULIP (The Uncorrected Long-read Integration Process), version 0.4 late 2016 (European eel)</p>
<p>TULIP currently consists of to Perl scripts, tulipseed.perl and tulipbulb.perl. These are very much intended as prototypes, and additional components and/or implementations are likely to follow.&nbsp;<br>Tulipseed takes as input alignments files of long reads to sparse short seeds, and outputs a graph and scaffold structures. Tulipbulb adds long read sequencing data to these.</p>
<p>&nbsp;</p>
<p>https://github.com/Generade-nl/TULIP</p><p>Address of the bookmark: <a href="https://github.com/Generade-nl/TULIP" rel="nofollow">https://github.com/Generade-nl/TULIP</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44470/phyloherb-phylogenomic-analysis-pipeline-for-herbarium-specimens</guid>
	<pubDate>Wed, 21 Feb 2024 06:15:13 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44470/phyloherb-phylogenomic-analysis-pipeline-for-herbarium-specimens</link>
	<title><![CDATA[PhyloHerb: Phylogenomic Analysis Pipeline for Herbarium Specimens]]></title>
	<description><![CDATA[<p><span>What is PhyloHerb</span><span>: PhyloHerb is a wrapper program to process&nbsp;</span><span>genome skimming</span><span>&nbsp;data collected from plant materials. The outcomes include the plastid genome (plastome) assemblies, mitochondrial genome assemblies, nuclear ribosomal DNAs (NTS+ETS+18S+ITS1+5.8S+ITS2+28S), alignments of gene and intergenic regions, and a species tree. It is designed to be a high throughput program dealing with lower quality data. Examples include&nbsp;</span><span>low-coverage (5x cpDNA) plastome phylogeny, recycling plastid genes from target enrichment data, retrieving low-copy nuclear genes from medium coverage (5x nucDNA) genome skimming</span><span>.</span></p><p>Address of the bookmark: <a href="https://github.com/lmcai/PhyloHerb/" rel="nofollow">https://github.com/lmcai/PhyloHerb/</a></p>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/news/view/26290/webinar-on-streamlining-large-scale-analysis-using-the-strand-ngs-pipeline-manager-on-24-feb-2016</guid>
	<pubDate>Fri, 05 Feb 2016 06:43:28 -0600</pubDate>
	<link>https://bioinformaticsonline.com/news/view/26290/webinar-on-streamlining-large-scale-analysis-using-the-strand-ngs-pipeline-manager-on-24-feb-2016</link>
	<title><![CDATA[Webinar on Streamlining large scale analysis using the Strand NGS Pipeline Manager on 24 Feb 2016]]></title>
	<description><![CDATA[<p><a href="http://www.strand-ngs.com/webinar_registration" title="webinar"><strong>Live Webinar on Streamlining large scale NGS data analysis using the Strand NGS Pipeline Manager on 24 Feb 2016</strong></a></p><p><strong>Abstract:</strong> Strand NGS includes comprehensive workflows for DNA-Seq, RNA-Seq, Small RNA-Seq, ChIP-Seq, MeDIP-Seq, and Methyl-Seq analysis. Each workflow includes a quality assessment and filter section, followed by a workflow-specific analysis section. The pipeline functionality in Strand NGS allows users to execute a sequence of analysis steps with specific parameters - all without any manual intervention. This simplifies the analysis in large scale sequencing projects where every sample needs to be processed identically.</p><p>In this webinar we will discuss the pre-packaged pipelines present in Strand NGS. The packaged pipelines have well-chosen default parameters and are suitable for users analyzing data for the first time in the tool. We will also show how advanced users can customize pipelines and share them with other Strand NGS users. Finally, we will show a brief glimpse of an elaborate pipeline that aligns reads, filters poor-quality matches, computes coverage metrics, identifies variants, checks for sample cross-contamination, and emails quality reports - all from within Strand NGS.</p><p><strong>Speaker:</strong> Dr. Vamsi Veeramachaneni, Vice President - Bioinformatics, Strand Life Sciences</p><p><strong>Details:</strong> Session 1: 2:30 PM IST, Session 2 : 10:30 PM IST<br /><strong>Register here:</strong> http://www.strand-ngs.com/webinar_registration</p><h3>&nbsp;</h3>]]></description>
	<dc:creator>Yeshodari</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37414/arc-pipeline-which-facilitates-iterative-reference-guided-de-novo-assemblies</guid>
	<pubDate>Thu, 26 Jul 2018 09:20:26 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37414/arc-pipeline-which-facilitates-iterative-reference-guided-de-novo-assemblies</link>
	<title><![CDATA[ARC: pipeline which facilitates iterative, reference guided de novo assemblies]]></title>
	<description><![CDATA[<p>ARC is a pipeline which facilitates iterative, reference guided&nbsp;<em>de novo</em>&nbsp;assemblies with the intent of:</p>
<ol>
<li>Reducing time in analysis and increasing accuracy of results by only considering those reads which should assemble together.</li>
<li>Reducing/removing reference bias as compared to mapping based approaches.</li>
</ol>
<p><span>The software is designed to work in situations where a whole-genome assembly is not the objective, but rather when the researcher wishes to assemble discreet 'targets' contained within next-generation shotgun sequence data. ARC decomplexifies the traditionally difficult problem of assembly by breaking the reads into small, manageable subsets which can then be assembled quickly and efficiently in parallel. Applications include those in which the researcher wishes to&nbsp;</span><em>de novo</em><span>&nbsp;assemble specific content and a set of semi-similar reference targets is available to initialize the assembly process.</span></p>
<p>https://ibest.github.io/ARC/</p><p>Address of the bookmark: <a href="https://ibest.github.io/ARC/" rel="nofollow">https://ibest.github.io/ARC/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/39370/multiphate-bioinformatics-pipeline-for-functional-annotation-of-phage-isolates</guid>
	<pubDate>Thu, 16 May 2019 00:17:39 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/39370/multiphate-bioinformatics-pipeline-for-functional-annotation-of-phage-isolates</link>
	<title><![CDATA[multiPhATE: bioinformatics pipeline for functional annotation of phage isolates]]></title>
	<description><![CDATA[<p><span>multiple-genome Phage Annotation Toolkit and Evaluator (multiPhATE). multiPhATE is a throughput pipeline driver that invokes an annotation pipeline (PhATE) across a user-specified set of phage genomes. This tool incorporates a&nbsp;</span><em>de novo</em><span>&nbsp;phage gene-calling algorithm and assigns putative functions to gene calls using protein-, virus-, and phage-centric databases.&nbsp;</span></p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="https://github.com/carolzhou/multiPhATE" rel="nofollow">https://github.com/carolzhou/multiPhATE</a></p>]]></description>
	<dc:creator>Abhimanyu Singh</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40611/deepvariant-an-analysis-pipeline-that-uses-a-deep-neural-network-to-call-genetic-variants-from-next-generation-dna-sequencing-data</guid>
	<pubDate>Sat, 25 Jan 2020 13:28:09 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40611/deepvariant-an-analysis-pipeline-that-uses-a-deep-neural-network-to-call-genetic-variants-from-next-generation-dna-sequencing-data</link>
	<title><![CDATA[DeepVariant : an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data.]]></title>
	<description><![CDATA[<p><span>DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data.</span></p>
<p><span><span>DeepVariant is an analysis pipeline that uses a deep neural network to call genetic variants from next-generation DNA sequencing data. DeepVariant relies on&nbsp;</span><a href="https://github.com/google/nucleus">Nucleus</a><span>, a library of Python and C++ code for reading and writing data in common genomics file formats (like SAM and VCF) designed for painless integration with the&nbsp;</span><a href="https://www.tensorflow.org/">TensorFlow</a><span>&nbsp;machine learning framework.</span></span></p>
<p><span><a href="https://ai.googleblog.com/2017/12/deepvariant-highly-accurate-genomes.html">https://ai.googleblog.com/2017/12/deepvariant-highly-accurate-genomes.html</a></span></p>
<p><span><a href="https://www.biorxiv.org/content/10.1101/092890v6">https://www.biorxiv.org/content/10.1101/092890v6</a></span></p>
<p><span><img src="https://4.bp.blogspot.com/-2KlXZO60sWE/WiGc8qlZfxI/AAAAAAAACOs/s1pNiKI8jsAvJLr1E_po5udDO8eObm_awCLcBGAs/s640/image3.png" width="640" height="427" alt="image" style="border: 0px;"></span></p><p>Address of the bookmark: <a href="https://github.com/google/deepvariant" rel="nofollow">https://github.com/google/deepvariant</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41998/wgddetector-a-pipeline-for-detecting-whole-genome-duplication-events-using-the-genome-or-transcriptome-annotations</guid>
	<pubDate>Thu, 23 Jul 2020 05:52:56 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41998/wgddetector-a-pipeline-for-detecting-whole-genome-duplication-events-using-the-genome-or-transcriptome-annotations</link>
	<title><![CDATA[WGDdetector: a pipeline for detecting whole genome duplication events using the genome or transcriptome annotations]]></title>
	<description><![CDATA[<p><span>WGDdetector pipeline that integrates all analyses including gene family constructing, dS estimating and phasing, and outputting the dS values of each paralogs pairs processed with only one command. We further chose four species (</span><em>Arabidopsis thaliana</em><span>,<span>&nbsp;</span></span><em>Juglans regia</em><span>,<span>&nbsp;</span></span><em>Populus trichocarpa</em><span><span>&nbsp;</span>and<span>&nbsp;</span></span><em>Xenopus laevis</em><span>) representing herb, wood and animal, to test its practicability. Our final results showed a high degree of accuracy with the previous studies using both genome and transcriptome data.</span></p>
<p><span>More at <a href="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-019-2670-3">https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-019-2670-3</a></span></p><p>Address of the bookmark: <a href="https://github.com/yongzhiyang2012/wgddetector" rel="nofollow">https://github.com/yongzhiyang2012/wgddetector</a></p>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/42946/aligngraph2-similar-genome-assisted-reassembly-pipeline-for-pacbio-long-reads</guid>
	<pubDate>Sun, 14 Mar 2021 09:42:47 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/42946/aligngraph2-similar-genome-assisted-reassembly-pipeline-for-pacbio-long-reads</link>
	<title><![CDATA[AlignGraph2: similar genome-assisted reassembly pipeline for PacBio long reads]]></title>
	<description><![CDATA[<p><span>AlignGraph2 is the second version of&nbsp;</span><a href="https://github.com/baoe/AlignGraph">AlignGraph</a><span>&nbsp;for PacBio long reads. It extends and refines contigs assembled from the long reads with a published genome similar to the sequencing genome.</span></p>
<p><span>More at&nbsp;https://academic.oup.com/bib/advance-article-abstract/doi/10.1093/bib/bbab022/6146772</span></p><p>Address of the bookmark: <a href="https://github.com/huangs001/AlignGraph2" rel="nofollow">https://github.com/huangs001/AlignGraph2</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44472/pipesnake-bioinformatics-best-practice-analysis-pipeline-for-phylogenomic-reconstruction</guid>
	<pubDate>Wed, 21 Feb 2024 06:19:41 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44472/pipesnake-bioinformatics-best-practice-analysis-pipeline-for-phylogenomic-reconstruction</link>
	<title><![CDATA[pipesnake: bioinformatics best-practice analysis pipeline for phylogenomic reconstruction]]></title>
	<description><![CDATA[<p dir="auto"><span>ausarg/pipesnake</span>&nbsp;is a bioinformatics best-practice analysis pipeline for phylogenomic reconstruction starting from short-read 'second-generation' sequencing data.</p>
<p dir="auto">The pipeline is built using&nbsp;<a href="https://www.nextflow.io/">Nextflow</a>, a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The&nbsp;<a href="https://www.nextflow.io/docs/latest/dsl2.html">Nextflow DSL2</a>&nbsp;implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies.</p><p>Address of the bookmark: <a href="https://github.com/AusARG/pipesnake" rel="nofollow">https://github.com/AusARG/pipesnake</a></p>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>

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