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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/43663?offset=10</link>
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	<description><![CDATA[]]></description>
	
	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/44320/tools-for-id-conversion</guid>
	<pubDate>Sat, 20 May 2023 21:53:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/44320/tools-for-id-conversion</link>
	<title><![CDATA[Tools for id conversion]]></title>
	<description><![CDATA[<p><strong>g:Convert</strong><span>&nbsp;enables to convert between various gene, protein, microarray probe and numerous other types of namespaces. We provide at least 40 types of IDs for more than 60 species. The 98 different namespaces supported for human include Ensembl, Refseq, Illumina, Entrezgene and Uniprot identifiers. All namespaces are obtained through matching them via Ensembl gene identifiers as a reference.</span></p><p>Address of the bookmark: <a href="https://biit.cs.ut.ee/gprofiler/convert" rel="nofollow">https://biit.cs.ut.ee/gprofiler/convert</a></p>]]></description>
	<dc:creator>LEGE</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32420/fastq-format</guid>
	<pubDate>Wed, 03 May 2017 04:23:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32420/fastq-format</link>
	<title><![CDATA[Fastq format]]></title>
	<description><![CDATA[<p><strong>FASTQ format</strong>&nbsp;is a text-based&nbsp;<a href="https://en.wikipedia.org/wiki/File_format" title="File format">format</a>&nbsp;for storing both a biological sequence (usually&nbsp;<a href="https://en.wikipedia.org/wiki/Nucleotide_sequence" title="Nucleotide sequence">nucleotide sequence</a>) and its corresponding quality scores. Both the sequence letter and quality score are each encoded with a single&nbsp;<a href="https://en.wikipedia.org/wiki/ASCII" title="ASCII">ASCII</a>&nbsp;character for brevity.</p>
<p>It was originally developed at the&nbsp;<a href="https://en.wikipedia.org/wiki/Wellcome_Trust_Sanger_Institute" title="Wellcome Trust Sanger Institute">Wellcome Trust Sanger Institute</a>&nbsp;to bundle a&nbsp;<a href="https://en.wikipedia.org/wiki/FASTA_format" title="FASTA format">FASTA</a>&nbsp;sequence and its quality data, but has recently become the&nbsp;<em>de facto</em>&nbsp;standard for storing the output of high-throughput sequencing instruments such as the&nbsp;<a href="https://en.wikipedia.org/wiki/Illumina_(company)" title="Illumina (company)">Illumina</a>&nbsp;Genome Analyzer.<sup id="cite_ref-Cock2009_1-0"><a href="https://en.wikipedia.org/wiki/FASTQ_format#cite_note-Cock2009-1">[1]</a></sup></p><p>Address of the bookmark: <a href="https://en.wikipedia.org/wiki/FASTQ_format" rel="nofollow">https://en.wikipedia.org/wiki/FASTQ_format</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/38004/vcfr-a-package-to-manipulate-and-visualize-vcf-data-in-r</guid>
	<pubDate>Thu, 25 Oct 2018 09:05:59 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/38004/vcfr-a-package-to-manipulate-and-visualize-vcf-data-in-r</link>
	<title><![CDATA[vcfR:  a package to manipulate and visualize VCF data in R]]></title>
	<description><![CDATA[<p><span>VcfR is an R package intended to allow easy manipulation and visualization of variant call format (VCF) data. Functions are provided to rapidly read from and write to VCF files. Once VCF data is read into R a parser function extracts matrices from the VCF data for use with typical R functions. This information can then be used for quality control or other purposes. Additional functions provide visualization of genomic data. Once processing is complete data may be written to a VCF file or converted into other popular R objects (e.g., genlight, DNAbin). VcfR provides a link between VCF data and the R environment connecting familiar software with genomic data.</span></p><p>Address of the bookmark: <a href="https://github.com/knausb/vcfR" rel="nofollow">https://github.com/knausb/vcfR</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/37396/converting-a-vcf-into-a-fasta-given-some-reference</guid>
	<pubDate>Fri, 20 Jul 2018 10:03:53 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/37396/converting-a-vcf-into-a-fasta-given-some-reference</link>
	<title><![CDATA[Converting a VCF into a FASTA given some reference !]]></title>
	<description><![CDATA[<p>Samtools/BCFtools (Heng Li) provides a Perl script&nbsp;<a href="https://github.com/lh3/samtools/blob/master/bcftools/vcfutils.pl"><code>vcfutils.pl</code></a>&nbsp;which does this, the function&nbsp;<code>vcf2fq</code>&nbsp;(lines 469-528)</p><p>This script has been modified by others to convert InDels as well, e.g.&nbsp;<a href="https://github.com/gringer/bioinfscripts/blob/master/vcf2fq.pl">this</a>&nbsp;by David Eccles</p><pre><code><span>./</span><span>vcf2fq</span><span>.</span><span>pl </span><span>-</span><span>f </span><span>&lt;</span><span>input</span><span>.</span><span>fasta</span><span>&gt;</span><span> </span><span>&lt;</span><span>all</span><span>-</span><span>site</span><span>.</span><span>vcf</span><span>&gt;</span><span> </span><span>&gt;</span><span> </span><span>&lt;</span><span>output</span><span>.</span><span>fastq</span><span>&gt;</span></code></pre><p>https://github.com/gringer/bioinfscripts/blob/master/vcf2fq.pl</p><p>https://github.com/lh3/samtools/blob/master/bcftools/vcfutils.pl</p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/28915/useful-bioinformatics-tools</guid>
	<pubDate>Mon, 29 Aug 2016 04:08:12 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/28915/useful-bioinformatics-tools</link>
	<title><![CDATA[Useful Bioinformatics Tools]]></title>
	<description><![CDATA[<p>Collections of few handy tools for bioinformatician</p>
<p>http://molbiol-tools.ca/Convert.htm</p><p>Address of the bookmark: <a href="http://molbiol-tools.ca/Convert.htm" rel="nofollow">http://molbiol-tools.ca/Convert.htm</a></p>]]></description>
	<dc:creator>Poonam Mahapatra</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/35144/converting-fastq-to-fasta</guid>
	<pubDate>Fri, 12 Jan 2018 03:49:09 -0600</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/35144/converting-fastq-to-fasta</link>
	<title><![CDATA[Converting FASTQ to FASTA]]></title>
	<description><![CDATA[<div id="block-system-main"><div><div><div><div><div><div><p>There are several ways you can convert fastq to fasta sequences. Some methods are listed below.</p><h3>Using SED</h3><p><span><code><span>sed</span></code></span>&nbsp;can be used to selectively print the desired lines from a file, so if you print the first and 2rd line of every 4 lines, you get the sequence header and sequence needed for fasta format.</p><pre>sed -n '1~4s/^@/&gt;/p;2~4p' INFILE.fastq &gt; OUTFILE.fasta
</pre><h3>Using PASTE</h3><p>You can linerize every 4 lines in a tabular format and print first and second field using&nbsp;<span><code>paste</code></span></p><pre>cat INFILE.fastq | paste - - - - |cut -f 1, 2| sed 's/@/&gt;/'g | tr -s "/t" "/n" &gt; OUTFILE.fasta
</pre><h3>EMBOSS:seqret</h3><p>Standard script that can be used for many purposes. One such use is fastq-fasta conversion</p><pre>seqret -sequence reads.fastq -outseq reads.fasta
</pre><p><span><code><span>awk</span></code></span>&nbsp;can be used for conversion as follows:</p><h3>Using AWK</h3><pre>cat infile.fq | awk '{if(NR%4==1) {printf("&gt;%s\n",substr($0,2));} else if(NR%4==2) print;}' &gt; file.fa
</pre><h3>FASTX-toolkit</h3><p><span><code>fastq_to_fasta</code></span>&nbsp;is available in the FASTX-toolkit that scales really well with the huge datasets</p><pre>fastq_to_fasta -h
usage: fastq_to_fasta [-h] [-r] [-n] [-v] [-z] [-i INFILE] [-o OUTFILE]
# Remember to use -Q33 for illumina reads!
version 0.0.6
       [-h]         = This helpful help screen.
       [-r]         = Rename sequence identifiers to numbers.
       [-n]         = keep sequences with unknown (N) nucleotides.
                   Default is to discard such sequences.
       [-v]         = Verbose - report number of sequences.
                   If [-o] is specified,  report will be printed to STDOUT.
                   If [-o] is not specified (and output goes to STDOUT),
                   report will be printed to STDERR.
       [-z]         = Compress output with GZIP.
       [-i INFILE]  = FASTA/Q input file. default is STDIN.
       [-o OUTFILE] = FASTA output file. default is STDOUT.
</pre><h3>Bioawk</h3><p>Another option to convert fastq to fasta format using&nbsp;<span><code>bioawk</code></span></p><pre>bioawk -c fastx '{print "&gt;"$name"\n"$seq}' input.fastq &gt; output.fasta
</pre><h3>Seqtk</h3><p>From the same developer, there is another option using a tool called&nbsp;<span><code>seqtk</code></span></p><pre>seqtk seq -a input.fastq &gt; output.fasta
</pre><p>Note that you can use either compressed or uncompressed files for this tool</p></div></div></div></div></div></div></div>]]></description>
	<dc:creator>Neel</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/29410/entrez-direct-e-utilities-on-the-unix-command-line</guid>
	<pubDate>Wed, 19 Oct 2016 08:06:24 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/29410/entrez-direct-e-utilities-on-the-unix-command-line</link>
	<title><![CDATA[Entrez Direct: E-utilities on the UNIX Command Line]]></title>
	<description><![CDATA[<p>Entrez Direct (EDirect) is an advanced method for accessing the NCBI's suite of interconnected databases (publication, sequence, structure, gene, variation, expression, etc.) from a UNIX terminal window. Functions take search terms from command-line arguments. Individual operations are combined to build multi-step queries. Record retrieval and formatting normally complete the process.</p>
<p>EDirect also provides an argument-driven function that simplifies the extraction of data from document summaries or other results that are returned in structured XML format. This can eliminate the need for writing custom software to answer ad hoc questions. Queries can move seamlessly between EDirect commands and UNIX utilities or scripts to perform actions that cannot be accomplished entirely within Entrez.</p><p>Address of the bookmark: <a href="https://www.ncbi.nlm.nih.gov/books/NBK179288/" rel="nofollow">https://www.ncbi.nlm.nih.gov/books/NBK179288/</a></p>]]></description>
	<dc:creator>Anjana</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/34699/biological-file-format-tutorial</guid>
	<pubDate>Sun, 17 Dec 2017 18:13:03 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/34699/biological-file-format-tutorial</link>
	<title><![CDATA[Biological file format tutorial]]></title>
	<description><![CDATA[<p>This section explains some of the commonly used file formats in bioinformatics. The information provided here is basic and designed to help users to distinguish the difference between different formats. Please refer user manual or other information resources on web for more details.</p>
<ol>
<li><a href="https://bioinformatics.uconn.edu/resources-and-events/tutorials/file-formats-tutorial/#fileformats_fasta">FASTA</a></li>
<li><a href="https://bioinformatics.uconn.edu/resources-and-events/tutorials/file-formats-tutorial/#fileformats_fastq">FASTQ</a></li>
<li><a href="https://bioinformatics.uconn.edu/resources-and-events/tutorials/file-formats-tutorial/#fileformats_sam">SAM</a></li>
<li><a href="https://bioinformatics.uconn.edu/resources-and-events/tutorials/file-formats-tutorial/#fileformats_bam">BAM</a></li>
<li><a href="https://bioinformatics.uconn.edu/resources-and-events/tutorials/file-formats-tutorial/#fileformats_vcf">VCF</a></li>
<li><a href="https://bioinformatics.uconn.edu/resources-and-events/tutorials/file-formats-tutorial/#fileformats_gff">GFF</a></li>
<li><a href="https://bioinformatics.uconn.edu/resources-and-events/tutorials/file-formats-tutorial/#fileformats_gtf">GTF</a></li>
</ol><p>Address of the bookmark: <a href="https://bioinformatics.uconn.edu/resources-and-events/tutorials/file-formats-tutorial/" rel="nofollow">https://bioinformatics.uconn.edu/resources-and-events/tutorials/file-formats-tutorial/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

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