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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/43870?offset=20</link>
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	<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27076/ale-a-generic-assembly-likelihood-evaluation-framework-for-assessing-the-accuracy-of-genome-and-metagenome-assemblies</guid>
	<pubDate>Tue, 26 Apr 2016 03:38:43 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27076/ale-a-generic-assembly-likelihood-evaluation-framework-for-assessing-the-accuracy-of-genome-and-metagenome-assemblies</link>
	<title><![CDATA[ALE: a Generic Assembly Likelihood Evaluation Framework for Assessing the Accuracy of Genome and Metagenome Assemblies]]></title>
	<description><![CDATA[<p>Assembly Likelihood Evaluation (ALE) framework that overcomes these limitations, systematically evaluating the accuracy of an assembly in a reference-independent manner using rigorous statistical methods. This framework is comprehensive, and integrates read quality, mate pair orientation and insert length (for paired-end reads), sequencing coverage, read alignment and k-mer frequency. ALE pinpoints synthetic errors in both single and metagenomic assemblies, including single-base errors, insertions/deletions, genome rearrangements and chimeric assemblies presented in metagenomes. At the genome level with real-world data, ALE identifies three large misassemblies from the Spirochaeta smaragdinae finished genome, which were all independently validated by Pacific Biosciences sequencing. At the single-base level with Illumina data, ALE recovers 215 of 222 (97%) single nucleotide variants in a training set from a GC-rich Rhodobacter sphaeroides genome. Using real Pacific Biosciences data, ALE identifies 12 of 12 synthetic errors in a Lambda Phage genome, surpassing even Pacific Biosciences' own variant caller, EviCons. In summary, the ALE framework provides a comprehensive, reference-independent and statistically rigorous measure of single genome and metagenome assembly accuracy, which can be used to identify misassemblies or to optimize the assembly process.</p>
<p>More at&nbsp;http://www.ncbi.nlm.nih.gov/pubmed/23303509</p><p>Address of the bookmark: <a href="http://sc932.github.io/ALE/about.html" rel="nofollow">http://sc932.github.io/ALE/about.html</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/42303/fqc-dashboard-integrates-fastqc-results-into-a-web-based-interactive-and-extensible-fastq-quality-control-tool</guid>
	<pubDate>Tue, 10 Nov 2020 01:30:22 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/42303/fqc-dashboard-integrates-fastqc-results-into-a-web-based-interactive-and-extensible-fastq-quality-control-tool</link>
	<title><![CDATA[FQC Dashboard: Integrates FastQC results into a web-based, interactive, and extensible FASTQ quality control tool]]></title>
	<description><![CDATA[<p>FQC is software that facilitates quality control of FASTQ files by carrying out a QC protocol using FastQC, parsing results, and aggregating quality metrics into an interactive dashboard designed to richly summarize individual sequencing runs. The dashboard groups samples in dropdowns for navigation among the data sets, utilizes human-readable configuration files to manipulate the pages and tabs, and is extensible with CSV data.</p><p>Address of the bookmark: <a href="https://github.com/pnnl/fqc" rel="nofollow">https://github.com/pnnl/fqc</a></p>]]></description>
	<dc:creator>Shruti Paniwala</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32420/fastq-format</guid>
	<pubDate>Wed, 03 May 2017 04:23:32 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32420/fastq-format</link>
	<title><![CDATA[Fastq format]]></title>
	<description><![CDATA[<p><strong>FASTQ format</strong>&nbsp;is a text-based&nbsp;<a href="https://en.wikipedia.org/wiki/File_format" title="File format">format</a>&nbsp;for storing both a biological sequence (usually&nbsp;<a href="https://en.wikipedia.org/wiki/Nucleotide_sequence" title="Nucleotide sequence">nucleotide sequence</a>) and its corresponding quality scores. Both the sequence letter and quality score are each encoded with a single&nbsp;<a href="https://en.wikipedia.org/wiki/ASCII" title="ASCII">ASCII</a>&nbsp;character for brevity.</p>
<p>It was originally developed at the&nbsp;<a href="https://en.wikipedia.org/wiki/Wellcome_Trust_Sanger_Institute" title="Wellcome Trust Sanger Institute">Wellcome Trust Sanger Institute</a>&nbsp;to bundle a&nbsp;<a href="https://en.wikipedia.org/wiki/FASTA_format" title="FASTA format">FASTA</a>&nbsp;sequence and its quality data, but has recently become the&nbsp;<em>de facto</em>&nbsp;standard for storing the output of high-throughput sequencing instruments such as the&nbsp;<a href="https://en.wikipedia.org/wiki/Illumina_(company)" title="Illumina (company)">Illumina</a>&nbsp;Genome Analyzer.<sup id="cite_ref-Cock2009_1-0"><a href="https://en.wikipedia.org/wiki/FASTQ_format#cite_note-Cock2009-1">[1]</a></sup></p><p>Address of the bookmark: <a href="https://en.wikipedia.org/wiki/FASTQ_format" rel="nofollow">https://en.wikipedia.org/wiki/FASTQ_format</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/35144/converting-fastq-to-fasta</guid>
	<pubDate>Fri, 12 Jan 2018 03:49:09 -0600</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/35144/converting-fastq-to-fasta</link>
	<title><![CDATA[Converting FASTQ to FASTA]]></title>
	<description><![CDATA[<div id="block-system-main"><div><div><div><div><div><div><p>There are several ways you can convert fastq to fasta sequences. Some methods are listed below.</p><h3>Using SED</h3><p><span><code><span>sed</span></code></span>&nbsp;can be used to selectively print the desired lines from a file, so if you print the first and 2rd line of every 4 lines, you get the sequence header and sequence needed for fasta format.</p><pre>sed -n '1~4s/^@/&gt;/p;2~4p' INFILE.fastq &gt; OUTFILE.fasta
</pre><h3>Using PASTE</h3><p>You can linerize every 4 lines in a tabular format and print first and second field using&nbsp;<span><code>paste</code></span></p><pre>cat INFILE.fastq | paste - - - - |cut -f 1, 2| sed 's/@/&gt;/'g | tr -s "/t" "/n" &gt; OUTFILE.fasta
</pre><h3>EMBOSS:seqret</h3><p>Standard script that can be used for many purposes. One such use is fastq-fasta conversion</p><pre>seqret -sequence reads.fastq -outseq reads.fasta
</pre><p><span><code><span>awk</span></code></span>&nbsp;can be used for conversion as follows:</p><h3>Using AWK</h3><pre>cat infile.fq | awk '{if(NR%4==1) {printf("&gt;%s\n",substr($0,2));} else if(NR%4==2) print;}' &gt; file.fa
</pre><h3>FASTX-toolkit</h3><p><span><code>fastq_to_fasta</code></span>&nbsp;is available in the FASTX-toolkit that scales really well with the huge datasets</p><pre>fastq_to_fasta -h
usage: fastq_to_fasta [-h] [-r] [-n] [-v] [-z] [-i INFILE] [-o OUTFILE]
# Remember to use -Q33 for illumina reads!
version 0.0.6
       [-h]         = This helpful help screen.
       [-r]         = Rename sequence identifiers to numbers.
       [-n]         = keep sequences with unknown (N) nucleotides.
                   Default is to discard such sequences.
       [-v]         = Verbose - report number of sequences.
                   If [-o] is specified,  report will be printed to STDOUT.
                   If [-o] is not specified (and output goes to STDOUT),
                   report will be printed to STDERR.
       [-z]         = Compress output with GZIP.
       [-i INFILE]  = FASTA/Q input file. default is STDIN.
       [-o OUTFILE] = FASTA output file. default is STDOUT.
</pre><h3>Bioawk</h3><p>Another option to convert fastq to fasta format using&nbsp;<span><code>bioawk</code></span></p><pre>bioawk -c fastx '{print "&gt;"$name"\n"$seq}' input.fastq &gt; output.fasta
</pre><h3>Seqtk</h3><p>From the same developer, there is another option using a tool called&nbsp;<span><code>seqtk</code></span></p><pre>seqtk seq -a input.fastq &gt; output.fasta
</pre><p>Note that you can use either compressed or uncompressed files for this tool</p></div></div></div></div></div></div></div>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/43376/hisat2-index-files-download</guid>
	<pubDate>Wed, 15 Sep 2021 22:17:49 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/43376/hisat2-index-files-download</link>
	<title><![CDATA[HISAT2 Index Files Download !]]></title>
	<description><![CDATA[<p>Resource for downloading all the HISAT2 related files&nbsp;</p>
<p>Please cite:</p>
<blockquote>
<p>Kim, D., Paggi, J.M., Park, C.&nbsp;<em>et al.</em>&nbsp;Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype.&nbsp;<em>Nat Biotechnol</em>&nbsp;<strong>37</strong>, 907&ndash;915 (2019).&nbsp;<a href="https://doi.org/10.1038/s41587-019-0201-4" target="_blank">https://doi.org/10.1038/s41587-019-0201-4</a></p>
</blockquote><p>Address of the bookmark: <a href="http://daehwankimlab.github.io/hisat2/download/#h-sapiens" rel="nofollow">http://daehwankimlab.github.io/hisat2/download/#h-sapiens</a></p>]]></description>
	<dc:creator>LEGE</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/40994/biological-databases</guid>
	<pubDate>Wed, 12 Feb 2020 01:16:29 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/40994/biological-databases</link>
	<title><![CDATA[Biological databases !]]></title>
	<description><![CDATA[<p>Now a days there are a lots of genomics databases available around the world. This bookmark is created to provide all links in one place ...</p>
<p>ftp://ftp.ncbi.nih.gov/genomes/</p>
<p>https://hgdownload.soe.ucsc.edu/downloads.html</p><p>Address of the bookmark: <a href="ftp://ftp.ncbi.nih.gov/genomes/" rel="nofollow">ftp://ftp.ncbi.nih.gov/genomes/</a></p>]]></description>
	<dc:creator>BioStar</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/41487/tinycov-standalone-command-line-utility-written-in-python-to-plot-coverage-from-a-bam-file</guid>
	<pubDate>Mon, 23 Mar 2020 06:22:08 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/41487/tinycov-standalone-command-line-utility-written-in-python-to-plot-coverage-from-a-bam-file</link>
	<title><![CDATA[tinycov: standalone command line utility written in python to plot coverage from a BAM file]]></title>
	<description><![CDATA[<p>Tinycov is a small standalone command line utility written in python to plot the coverage of a BAM file quickly. This software was inspired by&nbsp;<a href="https://github.com/matted/genome_coverage_plotter">Matt Edwards' genome coverage plotter</a>.</p>
<p>To install the stable version:&nbsp;<code>pip3 install --user tinycov</code></p>
<p>To install the development version:</p>
<pre><code>git clone https://github.com/cmdoret/tinycov.git
cd tinycov
pip install .</code></pre><p>Address of the bookmark: <a href="https://github.com/cmdoret/tinycov" rel="nofollow">https://github.com/cmdoret/tinycov</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37674/qualimap2-evaluating-next-generation-sequencing-alignment-data</guid>
	<pubDate>Tue, 11 Sep 2018 04:44:29 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37674/qualimap2-evaluating-next-generation-sequencing-alignment-data</link>
	<title><![CDATA[Qualimap2: Evaluating next generation sequencing alignment data]]></title>
	<description><![CDATA[<p><strong>Qualimap 2</strong><span>&nbsp;is a platform-independent application written in Java and R that provides both a Graphical User Inteface (GUI) and a command-line interface to facilitate the quality control of alignment sequencing data and its derivatives like feature counts.&nbsp;</span><br><br><span>Supported types of experiments include:</span></p>
<ul>
<li>Whole-genome sequencing</li>
<li>Whole-exome sequencing</li>
<li>RNA-seq (speical mode available)</li>
<li>ChIP-seq</li>
</ul><p>Address of the bookmark: <a href="http://qualimap.bioinfo.cipf.es/" rel="nofollow">http://qualimap.bioinfo.cipf.es/</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/27080/mrfast-micro-read-fast-alignment-search-tool</guid>
	<pubDate>Tue, 26 Apr 2016 03:50:06 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/27080/mrfast-micro-read-fast-alignment-search-tool</link>
	<title><![CDATA[mrFAST:  Micro Read Fast Alignment Search Tool]]></title>
	<description><![CDATA[<p><span>mrFAST is a read mapper that is designed to map short reads to reference genome with a special emphasis on the discovery of structural variation and segmental duplications. mrFAST maps short reads with respect to user defined error threshold, including indels up to 4+4 bp. This manual, describes how to choose the parameters and tune mrFAST with respect to the library settings. mrFAST is designed to find&nbsp;</span><strong><span style="text-decoration: underline;">'all'</span></strong><span>&nbsp; mappings for a given set of reads, however it can return one "best" map location if the relevant parameter is invoked.</span></p>
<p><span>More at&nbsp;http://mrfast.sourceforge.net/manual.html</span></p><p>Address of the bookmark: <a href="http://mrfast.sourceforge.net/manual.html" rel="nofollow">http://mrfast.sourceforge.net/manual.html</a></p>]]></description>
	<dc:creator>Neel</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/30085/fqtools</guid>
	<pubDate>Thu, 08 Dec 2016 09:31:12 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/30085/fqtools</link>
	<title><![CDATA[fqtools]]></title>
	<description><![CDATA[<p><code>fqtools</code><span>&nbsp;is a software suite for fast processing of&nbsp;</span><code>FASTQ</code><span>&nbsp;files. Various file manipulations are supported. See below for a full list of the subcommands available and a brief description of their purpose. Most of the individual subcommands will take either a single file or a pair of files as input. If no input file is specified, fqtools will attempt to read data from&nbsp;</span><code>stdin</code><span>. In this case, it is advisabe to specify the format of the data provided. For subcommands that generate FASTQ data, either a single file or a pair of files will be generated. If no&nbsp;</span><code>-o</code><span>&nbsp;argument is provided, single files will be writted to&nbsp;</span><code>stdout</code><span>.</span></p><p>Address of the bookmark: <a href="https://github.com/alastair-droop/fqtools" rel="nofollow">https://github.com/alastair-droop/fqtools</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

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