Address of the bookmark: https://github.com/vgl-hub/gfastats

What is Magic Wormhole?
Magic Wormhole is an open-source command-line tool that allows you to securely send files or text from one computer to another. Developed by Brian Warner, it aims to eliminate the usual hassle of file transfers: setting up SSH servers, dealing with firewall rules, cloud storage uploads, or even worrying about man-in-the-middle attacks.

Using a combination of PAKE (Password-Authenticated Key Exchange) protocols and end-to-end encryption, Magic Wormhole ensures that the only parties who can see your data are you and your recipient.

“It uses PAKE to establish a secure channel between two computers that use the same one-time code.”

How Does It Work?

One user runs a command like wormhole send file.txt.

The tool generates a human-readable, one-time code (like 7-horse-staple).

The other user types wormhole receive and enters the code.

The file is encrypted, transferred directly (or relayed if needed), and decrypted only on the recipient's side.

All of this happens over a secure channel, with no manual key exchange, configuration, or trust in a central authority.

Example Usage
# Sender
wormhole send myfile.pdf
Sending 1.4 MB file named 'myfile.pdf'
Wormhole code is: 7-horse-staple

# Receiver
wormhole receive
Please enter code: 7-horse-staple
Receiving file (1.4 MB) into: myfile.pdf

That’s it! No email attachments, no cloud storage, no FTP setups.

Why Use Magic Wormhole?
End-to-end encrypted transfers using modern cryptography.

Easy to use even for non-technical users.

Cross-platform: Works on Linux, macOS, and Windows.

No servers needed (except for a lightweight transit relay).

Works even behind NAT/firewalls.

It’s particularly ideal for:

Quickly sharing secrets or passwords.

Distributing software packages securely.

Moving files between servers or VMs.

Under the Hood
Magic Wormhole is written in Python and uses:

SPAKE2 for key exchange.

Transit relay and Mailbox server for message delivery.

Twisted framework for asynchronous networking.

The communication process is decentralized and designed to minimize the trust placed in the relay infrastructure. Even if an attacker intercepts the transit server, they cannot decrypt your data.

Installation

You can install it easily with pip:

pip install magic-wormhole

There’s also a Homebrew package for macOS users:

brew install magic-wormhole
Community and Ecosystem
Magic Wormhole is more than just a file transfer tool. It's part of a growing ecosystem that values user-centric cryptography. There are community-maintained libraries for other languages (e.g., Go, Rust), GUI frontends like wormhole-gui, and integration projects for mobile and web use.

Limitations

While Magic Wormhole is elegant and secure, it’s primarily a command-line utility and not designed for high-volume or persistent file sharing. Transfers require both sender and receiver to be online at the same time. And since it’s peer-to-peer, very large files may suffer performance issues.

Conclusion
Magic Wormhole is a breath of fresh air in the complex world of secure communication. It proves that cryptographic security doesn’t need to come with a heavy user experience cost. If you’re looking for a simple, secure, and delightful way to send files or messages, give Magic Wormhole a try.

Explore the documentation: https://magic-wormhole.readthedocs.io

This utility makes it easy to identify what are the properties of a read based on its SAM flag value, or conversely, to find what the SAM Flag value would be for a given combination of properties.

To decode a given SAM flag value, just enter the number in the field below. The encoded properties will be listed under Summary below, to the right.

Address of the bookmark: https://broadinstitute.github.io/picard/explain-flags.html

http://www.i-programmer.info/news/181-algorithms/4537-a-new-dna-sequence-search-compressive-genomics.html

http://en.wikipedia.org/wiki/Compression_of_Genomic_Re-Sequencing_Data

http://www.nature.com/nbt/journal/v30/n7/full/nbt.2241.html

http://bioinformatics.oxfordjournals.org/content/29/13/i283.full

http://groups.csail.mit.edu/cb/cast/

Canu is a hierachical assembly pipeline which runs in four steps:

• Detect overlaps in high-noise sequences using MHAP
• Generate corrected sequence consensus
• Trim corrected sequences
• Assemble trimmed corrected sequences

Read the documentation

New release https://github.com/marbl/canu/releases

Address of the bookmark: https://github.com/marbl/canu

Cleaning your data in this way is often required: Reads from small-RNA sequencing contain the 3’ sequencing adapter because the read is longer than the molecule that is sequenced. Amplicon reads start with a primer sequence. Poly-A tails are useful for pulling out RNA from your sample, but often you don’t want them to be in your reads.

Cutadapt helps with these trimming tasks by finding the adapter or primer sequences in an error-tolerant way. It can also modify and filter reads in various ways. Adapter sequences can contain IUPAC wildcard characters. Also, paired-end reads and even colorspace data is supported. If you want, you can also just demultiplex your input data, without removing adapter sequences at all.

Cutadapt comes with an extensive suite of automated tests and is available under the terms of the MIT license.

If you use cutadapt, please cite DOI:10.14806/ej.17.1.200 .

Address of the bookmark: https://cutadapt.readthedocs.io/en/stable/installation.html#quickstart

Address of the bookmark: https://github.com/OpenGene/AfterQC

Address of the bookmark: https://www.bioconductor.org/packages/release/bioc/vignettes/maftools/inst/doc/maftools.html