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	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/11457/commercial-and-public-next-gen-seq-ngs-software</guid>
	<pubDate>Tue, 03 Jun 2014 20:45:11 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/11457/commercial-and-public-next-gen-seq-ngs-software</link>
	<title><![CDATA[Commercial and public next-gen-seq (NGS) software]]></title>
	<description><![CDATA[<p><strong>Integrated solutions</strong><br /> <a href="http://www.clcbio.com/index.php?id=1240" target="_blank">CLCbio Genomics Workbench</a> - <em>de novo</em> and reference assembly of Sanger, Roche FLX, Illumina, Helicos, and SOLiD data. Commercial next-gen-seq software that extends the CLCbio Main Workbench software. Includes SNP detection, CHiP-seq, browser and other features. Commercial. Windows, Mac OS X and Linux.<br /><a href="http://g2.trac.bx.psu.edu/" target="_blank">Galaxy</a> - Galaxy = interactive and reproducible genomics. A job webportal.<br /> <a href="http://www.genomatix.de/products/index.html" target="_blank">Genomatix</a> - Integrated Solutions for Next Generation Sequencing data analysis.<br /> <a href="http://www.jmp.com/software/genomics/" target="_blank">JMP Genomics</a> - Next gen visualization and statistics tool from SAS. They are <a href="http://www.marketwatch.com/news/story/JMPR-Genomics-NCGR-Partnership-Foster/story.aspx?guid=%7B7AC9DE36-B6AA-4EDE-9CD5-633B29FE6154%7D" target="_blank">working with NCGR</a> to refine this tool and produce others.<br /> <a href="http://softgenetics.com/NextGENe.html" target="_blank">NextGENe</a> - <em>de novo</em> and reference assembly of Illumina, SOLiD and Roche FLX data. Uses a novel Condensation Assembly Tool approach where reads are joined via "anchors" into mini-contigs before assembly. Includes SNP detection, CHiP-seq, browser and other features. Commercial. Win or MacOS.<br /><a href="http://www.partek.com" target="_blank" title="Partek Incorporated">Partek</a>&nbsp;<span>- Commercial software for NGS, microarray, and qPCR data analysis. Streamlined analysis workflows for: ChIP-Seq, RNA-Seq, DNA-Seq, DNA Methylation, Gene Expression, Exon, miRNA Expression, Copy Number, Allele-Specific Copy Number, LOH, Association, Trio Analysis, and Tiling. Supports all commercial sequencing and microarray technologies.&nbsp;</span><br /> <a href="http://www.dnastar.com/products/SMGA.php" target="_blank">SeqMan Genome Analyser</a> - Software for Next Generation sequence assembly of Illumina, Roche FLX and Sanger data integrating with Lasergene Sequence Analysis software for additional analysis and visualization capabilities. Can use a hybrid templated/de novo approach. Commercial. Win or Mac OS X.<br /><a href="http://1001genomes.org/downloads/shore.html" target="_blank">SHORE</a> - SHORE, for Short Read, is a mapping and analysis pipeline for short DNA sequences produced on a Illumina Genome Analyzer. A suite created by the 1001 Genomes project. Source for POSIX.<br /> <a href="http://www.realtimegenomics.com/" target="_blank">SlimSearch</a> - Fledgling commercial product.<br />Synamatix has SXOligoSearch (<a href="http://synasite.mgrc.com.my:8080/sxog/NewSXOligoSearch.php" target="_blank">http://synasite.mgrc.com.my:8080/sxo...ligoSearch.php</a>)<br />The SWIFT suit is a software collection for fast index-based sequence comparison. It contains the following programs: SWIFT &mdash; fast local alignment search, guaranteeing to find epsilon-matches between two sequences; SWIFT BALSAM &mdash; a very fast program to find semiglobal non-gapped alignments based on k-mer seeds. <a href="http://bibiserv.techfak.uni-bielefeld.de/swift/" target="_blank">http://bibiserv.techfak.uni-bielefeld.de/swift/</a><br /><a href="http://http//bioinf.comav.upv.es/svn/biolib/biolib/src/" target="_blank">biolib</a>.is library and a set of script targeted to NGS. There are modules to: clean sequences (sanger, 454, ilumina), parse caf, ace and bowtie map files, clean and filter contigs, look for snps and indels., filter snps, do statistics for: reads, contigs and snps.</p><p><br /> <strong>Align/Assemble to a reference</strong><br /> <a href="https://secure.genome.ucla.edu/index.php/BFAST" target="_blank">BFAST</a> - Blat-like Fast Accurate Search Tool. Written by Nils Homer, Stanley F. Nelson and Barry Merriman at UCLA.<br /><a href="http://bowtie-bio.sourceforge.net/" target="_blank">Bowtie</a> - Ultrafast, memory-efficient short read aligner. It aligns short DNA sequences (reads) to the human genome at a rate of 25 million reads per hour on a typical workstation with 2 gigabytes of memory. Uses a Burrows-Wheeler-Transformed (BWT) index. <a href="http://seqanswers.com/forums/showthread.php?t=706" target="_blank">Link to discussion thread here</a>. Written by Ben Langmead and Cole Trapnell. Linux, Windows, and Mac OS X.<br /> <a href="http://maq.sourceforge.net/" target="_blank">BWA</a> - Heng Lee's BWT Alignment program - a progression from Maq. BWA is a fast light-weighted tool that aligns short sequences to a sequence database, such as the human reference genome. By default, BWA finds an alignment within edit distance 2 to the query sequence. C++ source.<br /> <a href="http://bioinfo.cgrb.oregonstate.edu/docs/solexa/" target="_blank">ELAND</a> - Efficient Large-Scale Alignment of Nucleotide Databases. Whole genome alignments to a reference genome. Written by Illumina author Anthony J. Cox for the Solexa 1G machine.<br /> <a href="http://www.ebi.ac.uk/%7Eguy/exonerate/" target="_blank">Exonerate</a> - Various forms of pairwise alignment (including Smith-Waterman-Gotoh) of DNA/protein against a reference. Authors are Guy St C Slater and Ewan Birney from EMBL. C for POSIX.<br /> <a href="http://1001genomes.org/downloads/genomemapper.html" target="_blank">GenomeMapper</a> - GenomeMapper is a short read mapping tool designed for accurate read alignments. It quickly aligns millions of reads either with ungapped or gapped alignments. A tool created by the 1001 Genomes project. Source for POSIX.<br /> <a href="http://www.gene.com/share/gmap/" target="_blank">GMAP</a> - GMAP (Genomic Mapping and Alignment Program) for mRNA and EST Sequences. Developed by Thomas Wu and Colin Watanabe at Genentec. C/Perl for Unix.<br /> <a href="http://dna.cs.byu.edu/gnumap/" target="_blank">gnumap</a> - The Genomic Next-generation Universal MAPper (gnumap) is a program designed to accurately map sequence data obtained from next-generation sequencing machines (specifically that of Solexa/Illumina) back to a genome of any size. It seeks to align reads from nonunique repeats using statistics. From authors at Brigham Young University. C source/Unix.<br /> <a href="http://sourceforge.net/projects/maq/" target="_blank">MAQ</a> - Mapping and Assembly with Qualities (renamed from MAPASS2). Particularly designed for Illumina with preliminary functions to handle ABI SOLiD data. Written by Heng Li from the Sanger Centre. Features extensive supporting tools for DIP/SNP detection, etc. C++ source<br /> <a href="http://bioinformatics.bc.edu/marthlab/Mosaik" target="_blank">MOSAIK</a> - MOSAIK produces gapped alignments using the Smith-Waterman algorithm. Features a number of support tools. Support for Roche FLX, Illumina, SOLiD, and Helicos. Written by Michael Str&ouml;mberg at Boston College. Win/Linux/MacOSX<br /> <a href="http://mrfast.sourceforge.net/" target="_blank">MrFAST and MrsFAST</a> - mrFAST &amp; mrsFAST are designed to map short reads generated with the Illumina platform to reference genome assemblies; in a fast and memory-efficient manner. Robust to INDELs and MrsFAST has a bisulphite mode. Authors are from the University of Washington. C as source.<br /> <a href="http://mummer.sourceforge.net/" target="_blank">MUMmer</a> - MUMmer is a modular system for the rapid whole genome alignment of finished or draft sequence. Released as a package providing an efficient suffix tree library, seed-and-extend alignment, SNP detection, repeat detection, and visualization tools. Version 3.0 was developed by Stefan Kurtz, Adam Phillippy, Arthur L Delcher, Michael Smoot, Martin Shumway, Corina Antonescu and Steven L Salzberg - most of whom are at The Institute for Genomic Research in Maryland, USA. POSIX OS required.<br /> <a href="http://www.novocraft.com/index.html" target="_blank">Novocraft</a> - Tools for reference alignment of paired-end and single-end Illumina reads. Uses a Needleman-Wunsch algorithm. Can support Bis-Seq. Commercial. Available free for evaluation, educational use and for use on open not-for-profit projects. Requires Linux or Mac OS X.<br /> <a href="http://pass.cribi.unipd.it/cgi-bin/pass.pl" target="_blank">PASS</a> - It supports Illumina, SOLiD and Roche-FLX data formats and allows the user to modulate very finely the sensitivity of the alignments. Spaced seed intial filter, then NW dynamic algorithm to a SW(like) local alignment. Authors are from CRIBI in Italy. Win/Linux.<br /> <a href="http://rulai.cshl.edu/rmap/" target="_blank">RMAP</a> - Assembles 20 - 64 bp Illumina reads to a FASTA reference genome. By Andrew D. Smith and Zhenyu Xuan at CSHL. (published in BMC Bioinformatics). POSIX OS required.<br /> <a href="http://biogibbs.stanford.edu/%7Ejiangh/SeqMap/" target="_blank">SeqMap</a> - Supports up to 5 or more bp mismatches/INDELs. Highly tunable. Written by Hui Jiang from the Wong lab at Stanford. Builds available for most OS's.<br /> <a href="http://compbio.cs.toronto.edu/shrimp/" target="_blank">SHRiMP</a> - Assembles to a reference sequence. Developed with Applied Biosystem's colourspace genomic representation in mind. Authors are Michael Brudno and Stephen Rumble at the University of Toronto. POSIX.<br /> <a href="http://www.bcgsc.ca/platform/bioinfo/software/slider" target="_blank"><span style="text-decoration: underline;">Slider</span></a>- An application for the Illumina Sequence Analyzer output that uses the probability files instead of the sequence files as an input for alignment to a reference sequence or a set of reference sequences. Authors are from BCGSC. Paper is <a href="http://seqanswers.com/forums/showthread.php?t=740" target="_blank">here</a>.<br /> <a href="http://soap.genomics.org.cn/" target="_blank">SOAP</a> - SOAP (Short Oligonucleotide Alignment Program). A program for efficient gapped and ungapped alignment of short oligonucleotides onto reference sequences. The updated version uses a BWT. Can call SNPs and INDELs. Author is Ruiqiang Li at the Beijing Genomics Institute. C++, POSIX.<br /> <a href="http://www.sanger.ac.uk/Software/analysis/SSAHA/" target="_blank">SSAHA</a> - SSAHA (Sequence Search and Alignment by Hashing Algorithm) is a tool for rapidly finding near exact matches in DNA or protein databases using a hash table. Developed at the Sanger Centre by Zemin Ning, Anthony Cox and James Mullikin. C++ for Linux/Alpha.<br /> <a href="http://socs.biology.gatech.edu/" target="_blank">SOCS</a> - Aligns SOLiD data. SOCS is built on an iterative variation of the Rabin-Karp string search algorithm, which uses hashing to reduce the set of possible matches, drastically increasing search speed. Authors are Ondov B, Varadarajan A, Passalacqua KD and Bergman NH.<br /> <a href="http://bibiserv.techfak.uni-bielefeld.de/swift/welcome.html" target="_blank">SWIFT</a> - The SWIFT suit is a software collection for fast index-based sequence comparison. It contains: SWIFT &mdash; fast local alignment search, guaranteeing to find epsilon-matches between two sequences. SWIFT BALSAM &mdash; a very fast program to find semiglobal non-gapped alignments based on k-mer seeds. Authors are Kim Rasmussen (SWIFT) and Wolfgang Gerlach (SWIFT BALSAM)<br /> <a href="http://synasite.mgrc.com.my:8080/sxog/NewSXOligoSearch.php" target="_blank">SXOligoSearch</a> - SXOligoSearch is a commercial platform offered by the Malaysian based <a href="http://www.synamatix.com/" target="_blank">Synamatix</a>. Will align Illumina reads against a range of Refseq RNA or NCBI genome builds for a number of organisms. Web Portal. OS independent.<br /> <a href="http://www.vmatch.de/" target="_blank">Vmatch</a> - A versatile software tool for efficiently solving large scale sequence matching tasks. Vmatch subsumes the software tool REPuter, but is much more general, with a very flexible user interface, and improved space and time requirements. Essentially a large string matching toolbox. POSIX.<br /> <a href="http://www.bioinformaticssolutions.com/products/zoom/index.php" target="_blank">Zoom</a> - ZOOM (Zillions Of Oligos Mapped) is designed to map millions of short reads, emerged by next-generation sequencing technology, back to the reference genomes, and carry out post-analysis. ZOOM is developed to be highly accurate, flexible, and user-friendly with speed being a critical priority. Commercial. Supports Illumina and SOLiD data.<br />NCGR uses GMAP (<a href="http://www.gene.com/share/gmap/" target="_blank">http://www.gene.com/share/gmap/</a>) to alignment Solexa reads. GMAP is free, though.<br />Exonerate (<a href="http://www.ebi.ac.uk/%7Eguy/exonerate/" target="_blank">http://www.ebi.ac.uk/~guy/exonerate/</a>)<br /> MUMmer (<a href="http://mummer.sourceforge.net/" target="_blank">http://mummer.sourceforge.net/</a>)<br /> The mapping short reads called gnumap (<a href="http://dna.cs.byu.edu/gnumap/" target="_blank">http://dna.cs.byu.edu/gnumap/</a>) made to increase the accuracy with duplicate matches. Open source, creates viewable output (with Affy's Integrated Genome Browser), and produces results very similar to novocraft's.<br /><a href="http://socs.biology.gatech.edu/" target="_blank">SOCS</a> (short oligonucleotides in color space)<br />BFAST <a href="https://secure.genome.ucla.edu/index.php/BFAST" target="_blank">https://secure.genome.ucla.edu/index.php/BFAST</a></p><p><br /> <strong><em>De novo</em> Align/Assemble</strong><br /> <a href="http://www.bcgsc.ca/platform/bioinfo/software/abyss" target="_blank">ABySS</a> - Assembly By Short Sequences. ABySS is a de novo sequence assembler that is designed for very short reads. The single-processor version is useful for assembling genomes up to 40-50 Mbases in size. The parallel version is implemented using MPI and is capable of assembling larger genomes. By Simpson JT and others at the Canada's Michael Smith Genome Sciences Centre. C++ as source. <br /> <a href="http://www.broad.mit.edu/science/programs/genome-biology/computational-rd/computational-research-and-development" target="_blank">ALLPATHS</a> - ALLPATHS: De novo assembly of whole-genome shotgun microreads. ALLPATHS is a whole genome shotgun assembler that can generate high quality assemblies from short reads. Assemblies are presented in a graph form that retains ambiguities, such as those arising from polymorphism, thereby providing information that has been absent from previous genome assemblies. Broad Institute.<br /> <a href="http://www.genomic.ch/edena.php" target="_blank">Edena</a> - Edena (Exact DE Novo Assembler) is an assembler dedicated to process the millions of very short reads produced by the Illumina Genome Analyzer. Edena is based on the traditional overlap layout paradigm. By D. Hernandez, P. Fran&ccedil;ois, L. Farinelli, M. Osteras, and J. Schrenzel. Linux/Win.<br /> <a href="http://euler-assembler.ucsd.edu/portal/" target="_blank">EULER-SR</a> - Short read <em>de novo</em> assembly. By Mark J. Chaisson and Pavel A. Pevzner from UCSD (published in Genome Research). Uses a de Bruijn graph approach.<br /> <a href="http://chevreux.org/projects_mira.html" target="_blank">MIRA2</a> - MIRA (Mimicking Intelligent Read Assembly) is able to perform true hybrid de-novo assemblies using reads gathered through 454 sequencing technology (GS20 or GS FLX). Compatible with 454, Solexa and Sanger data. Linux OS required.<br /> <a href="http://www.seqan.de/projects/consensus.html" target="_blank">SEQAN</a> - A Consistency-based Consensus Algorithm for De Novo and Reference-guided Sequence Assembly of Short Reads. By Tobias Rausch and others. C++, Linux/Win.<br /> <a href="http://sharcgs.molgen.mpg.de/" target="_blank">SHARCGS</a> - De novo assembly of short reads. Authors are Dohm JC, Lottaz C, Borodina T and Himmelbauer H. from the Max-Planck-Institute for Molecular Genetics.<br /> <a href="http://www.bcgsc.ca/platform/bioinfo/software/ssake" target="_blank">SSAKE</a> - The Short Sequence Assembly by K-mer search and 3' read Extension (SSAKE) is a genomics application for aggressively assembling millions of short nucleotide sequences by progressively searching for perfect 3'-most k-mers using a DNA prefix tree. Authors are Ren&eacute; Warren, Granger Sutton, Steven Jones and Robert Holt from the Canada's Michael Smith Genome Sciences Centre. Perl/Linux.<br /> <a href="http://soap.genomics.org.cn/" target="_blank">SOAPdenovo</a> - Part of the SOAP suite. See above. <br /> <a href="https://sourceforge.net/projects/vcake" target="_blank">VCAKE</a> - De novo assembly of short reads with robust error correction. An improvement on early versions of SSAKE.<br /> <a href="http://www.ebi.ac.uk/%7Ezerbino/velvet/" target="_blank">Velvet</a> - Velvet is a de novo genomic assembler specially designed for short read sequencing technologies, such as Solexa or 454. Need about 20-25X coverage and paired reads. Developed by Daniel Zerbino and Ewan Birney at the European Bioinformatics Institute (EMBL-EBI).<br />SOAP (<a href="http://soap.genomics.org.cn" target="_blank">http://soap.genomics.org.cn</a>) by Ruiqiang Li, as has been pointed by ECO.<br />Euler-SR (Euler-Short Reads Assembly, <a href="http://euler-assembler.ucsd.edu/portal/" target="_blank">http://euler-assembler.ucsd.edu/portal/</a>) by Mark J. Chaisson and Pavel A. Pevzner from UCSD. (published in Genome Research)<br />RMAP (A program for mapping Solexa reads, <a href="http://rulai.cshl.edu/rmap/" target="_blank">http://rulai.cshl.edu/rmap/</a>) by Andrew D. Smith and Zhenyu Xuan at CSHL. (published in BMC Bioinformatics)<br />Short read aligner called Bowtie (<a href="http://bowtie-bio.sourceforge.net/" target="_blank">http://bowtie-bio.sourceforge.net/</a>) designed for fast mapping of Illumina reads<br /> <br /> <strong>SNP/Indel Discovery</strong><br /> <a href="http://www.sanger.ac.uk/Software/analysis/ssahaSNP/" target="_blank">ssahaSNP</a> - ssahaSNP is a polymorphism detection tool. It detects homozygous SNPs and indels by aligning shotgun reads to the finished genome sequence. Highly repetitive elements are filtered out by ignoring those kmer words with high occurrence numbers. More tuned for ABI Sanger reads. Developers are Adam Spargo and Zemin Ning from the Sanger Centre. Compaq Alpha, Linux-64, Linux-32, Solaris and Mac<br /> <a href="http://bioinformatics.bc.edu/marthlab/PbShort" target="_blank">PolyBayesShort</a> - A re-incarnation of the PolyBayes SNP discovery tool developed by Gabor Marth at Washington University. This version is specifically optimized for the analysis of large numbers (millions) of high-throughput next-generation sequencer reads, aligned to whole chromosomes of model organism or mammalian genomes. Developers at Boston College. Linux-64 and Linux-32.<br /> <a href="http://bioinformatics.bc.edu/marthlab/PyroBayes" target="_blank">PyroBayes</a> - PyroBayes is a novel base caller for pyrosequences from the 454 Life Sciences sequencing machines. It was designed to assign more accurate base quality estimates to the 454 pyrosequences. Developers at Boston College.<br />Maq is also able to find SNPs with its own alignment. It has a graphical viewer, but again for its own alignment format.<br />SSAHA has been optimized for short-reads, too. But yes, SSAHASNP appears in your "SNP/INDEL discovery" category.<br /> <br /> <strong>Genome Annotation/Genome Browser/Alignment Viewer/Assembly Database</strong><br /> <a href="http://bioinformatics.bc.edu/marthlab/EagleView" target="_blank">EagleView</a> - An information-rich genome assembler viewer. EagleView can display a dozen different types of information including base quality and flowgram signal. Developers at Boston College.<br /> <a href="http://www.sanger.ac.uk/Software/analysis/lookseq/" target="_blank">LookSeq</a> - LookSeq is a web-based application for alignment visualization, browsing and analysis of genome sequence data. LookSeq supports multiple sequencing technologies, alignment sources, and viewing modes; low or high-depth read pileups; and easy visualization of putative single nucleotide and structural variation. From the Sanger Centre.<br /> <a href="http://evolution.sysu.edu.cn/mapview/" target="_blank">MapView</a> - MapView: visualization of short reads alignment on desktop computer. From the Evolutionary Genomics Lab at Sun-Yat Sen University, China. Linux.<br /> <a href="http://www.bcgsc.ca/platform/bioinfo/software/sam" target="_blank">SAM</a> - Sequence Assembly Manager. Whole Genome Assembly (WGA) Management and Visualization Tool. It provides a generic platform for manipulating, analyzing and viewing WGA data, regardless of input type. Developers are Rene Warren, Yaron Butterfield, Asim Siddiqui and Steven Jones at Canada's Michael Smith Genome Sciences Centre. MySQL backend and Perl-CGI web-based frontend/Linux. <br /> <a href="http://staden.sourceforge.net/" target="_blank">STADEN</a> - Includes GAP4. GAP5 once completed will handle next-gen sequencing data. A partially implemented test version is available <a href="https://sourceforge.net/project/show...kage_id=256957" target="_blank">here</a><br /> <a href="http://www.bcgsc.ca/platform/bioinfo/software/xmatchview" target="_blank">XMatchView</a> - A visual tool for analyzing cross_match alignments. Developed by Rene Warren and Steven Jones at Canada's Michael Smith Genome Sciences Centre. Python/Win or Linux.<br /> <br /> <strong>Counting e.g. CHiP-Seq, Bis-Seq, CNV-Seq</strong><br /> <a href="http://epigenomics.mcdb.ucla.edu/BS-Seq/download.html" target="_blank">BS-Seq</a> - The source code and data for the "Shotgun Bisulphite Sequencing of the Arabidopsis Genome Reveals DNA Methylation Patterning" Nature paper by <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?holding=&amp;db=pubmed&amp;cmd=search&amp;term=Shotgun%20Bisulphite%20Sequencing" target="_blank">Cokus et al.</a> (Steve Jacobsen's lab at UCLA). POSIX.<br /> <a href="http://woldlab.caltech.edu/chipseq/" target="_blank">CHiPSeq</a> - Program used by Johnson et al. (2007) in their Science publication<br /> <a href="http://tiger.dbs.nus.edu.sg/cnv-seq/" target="_blank">CNV-Seq</a> - CNV-seq, a new method to detect copy number variation using high-throughput sequencing. Chao Xie and Martti T Tammi at the National University of Singapore. Perl/R.<br /> <a href="http://www.bcgsc.ca/platform/bioinfo/software/findpeaks" target="_blank">FindPeaks</a> - perform analysis of ChIP-Seq experiments. It uses a naive algorithm for identifying regions of high coverage, which represent Chromatin Immunoprecipitation enrichment of sequence fragments, indicating the location of a bound protein of interest. Original algorithm by Matthew Bainbridge, in collaboration with Gordon Robertson. Current code and implementation by Anthony Fejes. Authors are from the Canada's Michael Smith Genome Sciences Centre. JAVA/OS independent. Latest versions available as part of the <a href="http://vancouvershortr.sourceforge.net/" target="_blank">Vancouver Short Read Analysis Package</a><br /> <a href="http://liulab.dfci.harvard.edu/MACS/" target="_blank">MACS</a> - Model-based Analysis for ChIP-Seq. MACS empirically models the length of the sequenced ChIP fragments, which tends to be shorter than sonication or library construction size estimates, and uses it to improve the spatial resolution of predicted binding sites. MACS also uses a dynamic Poisson distribution to effectively capture local biases in the genome sequence, allowing for more sensitive and robust prediction. Written by Yong Zhang and Tao Liu from Xiaole Shirley Liu's Lab. <br /> <a href="http://www.gersteinlab.org/proj/PeakSeq/" target="_blank">PeakSeq</a> - PeakSeq: Systematic Scoring of ChIP-Seq Experiments Relative to Controls. a two-pass approach for scoring ChIP-Seq data relative to controls. The first pass identifies putative binding sites and compensates for variation in the mappability of sequences across the genome. The second pass filters out sites that are not significantly enriched compared to the normalized input DNA and computes a precise enrichment and significance. By Rozowsky J et al. C/Perl.<br /> <a href="http://mendel.stanford.edu/sidowlab/downloads/quest/" target="_blank">QuEST</a> - Quantitative Enrichment of Sequence Tags. Sidow and Myers Labs at Stanford. From the 2008 publication <a href="http://www.ncbi.nlm.nih.gov/pubmed/18711362" target="_blank">Genome-wide analysis of transcription factor binding sites based on ChIP-Seq data</a>. (C++)<br /> <a href="http://dir.nhlbi.nih.gov/papers/lmi/epigenomes/sissrs/" target="_blank">SISSRs</a> - Site Identification from Short Sequence Reads. BED file input. Raja Jothi @ NIH. Perl.<br />SeqMap (<a href="http://biogibbs.stanford.edu/%7Ejiangh/SeqMap/" target="_blank">http://biogibbs.stanford.edu/~jiangh/SeqMap/</a>) - work like ELand, can do 3 or more bp mismatches and also insdel<br />ChIPSeq analysis is:&nbsp; <a href="http://dir.nhlbi.nih.gov/papers/lmi/epigenomes/sissrs/" target="_blank">http://dir.nhlbi.nih.gov/papers/lmi/epigenomes/sissrs/</a></p><p>See also <a href="http://seqanswers.com/forums/showthread.php?t=742" target="_blank">this thread</a> for ChIP-Seq, until I get time to update this list.<br /> <br /> <strong>Alternate Base Calling</strong><br /> <a href="http://svitsrv25.epfl.ch/R-doc/library/Rolexa/html/00Index.html" target="_blank">Rolexa</a> - R-based framework for base calling of Solexa data. Project <a href="http://www.biomedcentral.com/1471-2105/9/431" target="_blank">publication</a><br /> <a href="http://hannonlab.cshl.edu/Alta-Cyclic/main.html" target="_blank">Alta-cyclic</a> - "a novel Illumina Genome-Analyzer (Solexa) base caller"<br /> <br /> <strong>Transcriptomics</strong><br /> <a href="http://woldlab.caltech.edu/rnaseq/" target="_blank">ERANGE</a> - Mapping and Quantifying Mammalian Transcriptomes by RNA-Seq. Supports Bowtie, BLAT and ELAND. From the Wold lab.<br /> <a href="http://www.genoscope.cns.fr/externe/gmorse/" target="_blank">G-Mo.R-Se</a> - G-Mo.R-Se is a method aimed at using RNA-Seq short reads to build de novo gene models. First, candidate exons are built directly from the positions of the reads mapped on the genome (without any ab initio assembly of the reads), and all the possible splice junctions between those exons are tested against unmapped reads. From CNS in France.<br /> <a href="http://evolution.sysu.edu.cn/english/software/mapnext.htm" target="_blank">MapNext</a> - MapNext: A software tool for spliced and unspliced alignments and SNP detection of short sequence reads. From the Evolutionary Genomics Lab at Sun-Yat Sen University, China.<br /> <a href="http://www.fml.tuebingen.mpg.de/raetsch/suppl/qpalma" target="_blank">QPalma</a> - Optimal Spliced Alignments of Short Sequence Reads. Authors are Fabio De Bona, Stephan Ossowski, Korbinian Schneeberger, and Gunnar R&auml;tsch. A paper is <a href="http://www.fml.tuebingen.mpg.de/raetsch/suppl/qpalma/qpalma-final.pdf" target="_blank">available</a>.<br /> <a href="http://biogibbs.stanford.edu/%7Ejiangh/rsat/" target="_blank">RSAT</a> - RSAT: RNA-Seq Analysis Tools. RNASAT is developed and maintained by Hui Jiang at Stanford University.<br /> <a href="http://tophat.cbcb.umd.edu/" target="_blank">TopHat</a> - TopHat is a fast splice junction mapper for RNA-Seq reads. It aligns RNA-Seq reads to mammalian-sized genomes using the ultra high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between exons. TopHat is a collaborative effort between the University of Maryland and the University of California, Berkeley<br />NGS-Trex: Next Generation Sequencing Transcriptome profile explorer http://www.biomedcentral.com/1471-2105/14/S7/S10</p><p>Reference</p><p>Illumina has a software list: <a href="http://www.illumina.com/pagesnrn.ilmn?ID=245" target="_blank">http://www.illumina.com/pagesnrn.ilmn?ID=245</a>.</p><p>Some softwares in his blog (<a href="http://www.fejes.ca/labels/DNA.html" target="_blank">http://www.fejes.ca/labels/DNA.html</a>)</p><p><a href="http://seqanswers.com/wiki/Software" target="_blank">http://seqanswers.com/wiki/Software</a></p>]]></description>
	<dc:creator>Surabhi Chaudhary</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/26356/spines</guid>
	<pubDate>Tue, 09 Feb 2016 05:07:15 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/26356/spines</link>
	<title><![CDATA[Spines]]></title>
	<description><![CDATA[<div id="content-header">
<h1>Spines</h1>
</div>
<div id="node-1301">
<div>
<div>
<p><a href="http://www.broadinstitute.org/ftp/distribution/software/spines/"><em>Spines</em></a> is a collection of software tools, developed and used by the Vertebrate Genome Biology Group at the Broad Institute. It provides basic data structures for efficient data manipulation (mostly genomic sequences, alignments, variation etc.), as well as specialized tool sets for various analyses. It also features three sequence alignment packages: <em>Satsuma,</em> a highly parallelized program for high-sensitivity, genome-wide synteny; <em>Papaya,</em> an all-purpose alignment tool for less diverged sequences; and <em>SLAP,</em> a context-sensitive local aligner for diverged sequences with large gaps.</p>
<p>Access <em>Spines</em> <a href="http://www.broadinstitute.org/ftp/distribution/software/spines/">here</a>.</p>
</div>
</div>
</div>
<p>http://www.broadinstitute.org/science/programs/genome-biology/spines</p><p>Address of the bookmark: <a href="http://www.broadinstitute.org/science/programs/genome-biology/spines" rel="nofollow">http://www.broadinstitute.org/science/programs/genome-biology/spines</a></p>]]></description>
	<dc:creator>Jitendra Narayan</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/26852/awesome-bioinformatics-pipelines</guid>
	<pubDate>Wed, 30 Mar 2016 21:50:41 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/26852/awesome-bioinformatics-pipelines</link>
	<title><![CDATA[Awesome bioinformatics pipelines !]]></title>
	<description><![CDATA[<p><span>A curated list of awesome pipeline toolkits ...</span></p>
<p><span>https://github.com/pditommaso/awesome-pipeline</span></p><p>Address of the bookmark: <a href="https://github.com/pditommaso/awesome-pipeline" rel="nofollow">https://github.com/pditommaso/awesome-pipeline</a></p>]]></description>
	<dc:creator>Jitendra Prajapati</dc:creator>
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<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/blog/view/42188/tools-and-method-for-haplotype-phasing</guid>
	<pubDate>Fri, 04 Sep 2020 20:41:40 -0500</pubDate>
	<link>https://bioinformaticsonline.com/blog/view/42188/tools-and-method-for-haplotype-phasing</link>
	<title><![CDATA[Tools and Method for Haplotype phasing !]]></title>
	<description><![CDATA[<div>Huge amounts of genotype data are being produced with recent technological advances, both from increasingly&nbsp; comprehensive and inexpensive genome-wide SNP microarrays and from ever more accessible whole-genome and whole-exome sequencing methods. The vast amount of knowledge contained in these results, however, is best&nbsp; exploited through phased haplotypes, which classify the alleles co-located on the same chromosome. Since sequence and SNP array data normally take the form of unphased genotypes, one does not specifically observe which of the two parental chromosomes, or haplotypes, falls on a specific allele. Fortunately, new advances in both computational and laboratory methods promise improved determination of haplotype phase. Following are useful tools :</div><div>&nbsp;</div><p><strong>Arlequin:</strong>&nbsp;<a href="http://cmpg.unibe.ch/software/arlequin3/" target="_blank">http://cmpg.unibe.ch/software/arlequin3/</a></p><p><strong>BEAGLE:</strong>&nbsp;<a href="http://faculty.washington.edu/browning/beagle/beagle.html" target="_blank">http://faculty.washington.edu/browning/beagle/beagle.html</a></p><p><strong>fastPHASE:</strong>&nbsp;<a href="http://stephenslab.uchicago.edu/software.html" target="_blank">http://stephenslab.uchicago.edu/software.html</a></p><p><strong>GENEHUNTER:</strong>&nbsp;<a href="http://linkage.rockefeller.edu/soft/gh/" target="_blank">http://linkage.rockefeller.edu/soft/gh/</a></p><p><strong>The Genome Analysis Toolkit:</strong></p><p><a href="http://www.broadinstitute.org/gsa/wiki/index.php/The_Genome_Analysis_Toolkit" target="_blank">http://www.broadinstitute.org/gsa/wiki/index.php/The_Genome_Analysis_Toolkit</a></p><p><strong>IMPUTE2:</strong>&nbsp;<a href="https://mathgen.stats.ox.ac.uk/impute/impute_v2.html" target="_blank">https://mathgen.stats.ox.ac.uk/impute/impute_v2.html</a></p><p><strong>MACH:</strong>&nbsp;<a href="http://www.sph.umich.edu/csg/abecasis/MACH/" target="_blank">http://www.sph.umich.edu/csg/abecasis/MACH/</a></p><p><strong>MERLIN:</strong>&nbsp;<a href="http://www.sph.umich.edu/csg/abecasis/Merlin/" target="_blank">http://www.sph.umich.edu/csg/abecasis/Merlin/</a></p><p><strong>PHASE:</strong>&nbsp;<a href="http://stephenslab.uchicago.edu/software.html" target="_blank">http://stephenslab.uchicago.edu/software.html</a></p><p><strong>PL-EM:</strong>&nbsp;<a href="http://www.people.fas.harvard.edu/~junliu/plem/" target="_blank">http://www.people.fas.harvard.edu/~junliu/plem/</a></p><p><strong>&ldquo;Read-backed phasing&rdquo; algorithm</strong>:&nbsp;<a href="http://www.broadinstitute.org/gsa/wiki/index.php/Read-backed_phasing_algorithm" target="_blank">http://www.broadinstitute.org/gsa/wiki/index.php/Read-backed_phasing_algorithm</a></p><p><strong>SHAPE-IT:</strong>&nbsp;<a href="http://www.griv.org/shapeit/" target="_blank">http://www.griv.org/shapeit/</a></p>]]></description>
	<dc:creator>Manisha Mishra</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/30336/finding-patterns-in-biological-sequences</guid>
	<pubDate>Thu, 22 Dec 2016 10:30:49 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/30336/finding-patterns-in-biological-sequences</link>
	<title><![CDATA[Finding Patterns in Biological Sequences]]></title>
	<description><![CDATA[<p>In this report we provide an overview of known techniques for discovery of patterns of biological sequences (DNA and proteins). We also provide biological motivation, and methods of biological verification of such patterns. Finally we list publicly available tools and databases for pattern discovery. On-line supplement is available through http://genetics.uwaterloo.ca/&sim;tvinar/cs798g/motif.</p><p>Address of the bookmark: <a href="http://engr.case.edu/li_jing/papers/00798gpattern.pdf" rel="nofollow">http://engr.case.edu/li_jing/papers/00798gpattern.pdf</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/32131/wgs-celera-assembler-version-83rc2</guid>
	<pubDate>Mon, 10 Apr 2017 04:45:40 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/32131/wgs-celera-assembler-version-83rc2</link>
	<title><![CDATA[WGS Celera Assembler version 8.3rc2]]></title>
	<description><![CDATA[<p>These are release notes for Celera Assembler version 8.3rc2, which was released on May 24, 2015.<br><br>This distribution package provides a stable, tested, documented version of the software.&nbsp; The distribution is usable on most Unix-like platforms, and some platforms have pre-compiled binary distributions ready for installation.<br><br>The source code package includes full source code (revision 4627), Makefiles, and scripts.&nbsp; A subset of the kmer package (http://kmer.sourceforge.net/, version r1994), used by some modules of Celera Assembler, is included.&nbsp; This distribution includes [http://samtools.sourceforge.net/ SAMtools], [http://www.cbcb.umd.edu/software/jellyfish/ Jellyfish 2.0], [https://github.com/pbjd/pbutgcns PBUTGCNS], [https://github.com/PacificBiosciences/pbdagcon PBDAGCON], [https://github.com/PacificBiosciences/BLASR BLASR], and parts of the [https://github.com/PacificBiosciences/FALCON/tree/v0.1.3 Falcon assembler].<br><br>Full documentation can be found online at http://wgs-assembler.sourceforge.net/.</p>
<p>Interesting scripts within it</p>
<p>urbe@urbo214b[bin] ls&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; []<br>-rwxrwxr-x 1 urbe urbe&nbsp; 11K Apr 10 11:41 addCNSToStore<br>-rwxrwxr-x 1 urbe urbe 575K Apr 10 11:41 addReadsToUnitigs<br>-rwxrwxr-x 1 urbe urbe 128K Apr 10 11:41 analyzeBest<br>-rwxrwxr-x 1 urbe urbe 257K Apr 10 11:41 analyzePosMap<br>-rwxrwxr-x 1 urbe urbe 1,5M Apr 10 11:41 analyzeScaffolds<br>-rwxrwxr-x 1 urbe urbe 224K Apr 10 11:41 asmOutputFasta<br>-rwxrwxr-x 1 urbe urbe 448K Apr 10 11:41 asmOutputStatistics<br>-rwxrwxr-x 1 urbe urbe 2,4K Apr 10 11:41 asmToAGP.pl<br>-rwxrwxr-x 1 urbe urbe 7,6M Apr 10 11:41 blasr<br>-rwxrwxr-x 1 urbe urbe 1,6M Apr 10 11:41 bogart<br>-rwxrwxr-x 1 urbe urbe 183K Apr 10 11:41 bogus<br>-rwxrwxr-x 1 urbe urbe 272K Apr 10 11:41 bogusness<br>-rwxrwxr-x 1 urbe urbe 247K Apr 10 11:41 buildPosMap<br>-rwxrwxr-x 1 urbe urbe 213K Apr 10 11:41 buildRefContigs<br>-rwxrwxr-x 1 urbe urbe 990K Apr 10 11:41 buildUnitigs<br>-rwxrwxr-x 1 urbe urbe&nbsp; 18K Apr 10 11:41 ca2ace.pl<br>-rwxrwxr-x 1 urbe urbe&nbsp; 12K Apr 10 11:41 caqc_help.ini<br>-rwxrwxr-x 1 urbe urbe&nbsp; 61K Apr 10 11:41 caqc.pl<br>-rwxrwxr-x 1 urbe urbe&nbsp; 23K Apr 10 11:41 cat-corrects<br>-rwxrwxr-x 1 urbe urbe&nbsp; 24K Apr 10 11:41 cat-erates<br>-rwxrwxr-x 1 urbe urbe 1,9M Apr 10 11:41 cgw<br>-rwxrwxr-x 1 urbe urbe 1,4M Apr 10 11:41 cgwDump<br>-rwxrwxr-x 1 urbe urbe 204K Apr 10 11:41 chimChe<br>-rwxrwxr-x 1 urbe urbe 201K Apr 10 11:40 chimera<br>-rwxrwxr-x 1 urbe urbe 220K Apr 10 11:41 classifyMates<br>-rwxrwxr-x 1 urbe urbe 201K Apr 10 11:41 classifyMatesApply<br>-rwxrwxr-x 1 urbe urbe 215K Apr 10 11:41 classifyMatesPairwise<br>-rwxrwxr-x 1 urbe urbe 366K Apr 10 11:41 computeCoverageStat<br>-rwxrwxr-x 1 urbe urbe 9,8K Apr 10 11:41 convert-fasta-to-v2.pl<br>-rwxrwxr-x 1 urbe urbe&nbsp; 48K Apr 10 11:41 convertOverlap<br>-rwxrwxr-x 1 urbe urbe 119K Apr 10 11:41 convertSamToCA<br>-rwxrwxr-x 1 urbe urbe&nbsp; 20K Apr 10 11:41 convertToPBCNS<br>-rwxrwxr-x 1 urbe urbe 197K Apr 10 11:41 correct-frags<br>-rwxrwxr-x 1 urbe urbe 259K Apr 10 11:41 correct-olaps<br>-rwxrwxr-x 1 urbe urbe 520K Apr 10 11:41 correctPacBio<br>-rwxrwxr-x 1 urbe urbe 540K Apr 10 11:41 ctgcns<br>-rwxrwxr-x 1 urbe urbe 162K Apr 10 11:40 deduplicate<br>-rwxrwxr-x 1 urbe urbe&nbsp; 37K Apr 10 11:41 demotePosMap<br>-rwxrwxr-x 1 urbe urbe 1,5M Apr 10 11:41 dumpCloneMiddles<br>-rwxrwxr-x 1 urbe urbe 124K Apr 10 11:41 dumpPBRLayoutStore<br>-rwxrwxr-x 1 urbe urbe 1,3M Apr 10 11:41 dumpSingletons<br>-rwxrwxr-x 1 urbe urbe 171K Apr 10 11:41 erate-estimate<br>-rwxrwxr-x 1 urbe urbe 221K Apr 10 11:40 estimate-mer-threshold<br>-rwxrwxr-x 1 urbe urbe 1,5M Apr 10 11:41 extendClearRanges<br>-rwxrwxr-x 1 urbe urbe 1,3M Apr 10 11:41 extendClearRangesPartition<br>-rwxrwxr-x 1 urbe urbe 205K Apr 10 11:40 extractmessages<br>-rwxrwxr-x 1 urbe urbe 7,2M Apr 10 11:41 falcon_sense<br>-rwxrwxr-x 1 urbe urbe 9,8K Apr 10 11:41 fastaToCA<br>-rwxrwxr-x 1 urbe urbe 124K Apr 10 11:40 fastqAnalyze<br>-rwxrwxr-x 1 urbe urbe 137K Apr 10 11:40 fastqSample<br>-rwxrwxr-x 1 urbe urbe&nbsp; 62K Apr 10 11:40 fastqSimulate<br>-rwxrwxr-x 1 urbe urbe 121K Apr 10 11:40 fastqSimulate-sort<br>-rwxrwxr-x 1 urbe urbe 246K Apr 10 11:40 fastqToCA<br>-rwxrwxr-x 1 urbe urbe 140K Apr 10 11:41 filterOverlap<br>-rwxrwxr-x 1 urbe urbe 341K Apr 10 11:40 finalTrim<br>-rwxrwxr-x 1 urbe urbe 228K Apr 10 11:41 fixUnitigs<br>-rwxrwxr-x 1 urbe urbe 147K Apr 10 11:40 fragmentDepth<br>-rwxrwxr-x 1 urbe urbe&nbsp; 29K Apr 10 11:41 fragsInVars<br>-rwxrwxr-x 1 urbe urbe 545K Apr 10 11:41 frgs2clones<br>-rwxrwxr-x 1 urbe urbe 398K Apr 10 11:40 gatekeeper<br>-rwxrwxr-x 1 urbe urbe 139K Apr 10 11:40 gatekeeperbench<br>-rwxrwxr-x 1 urbe urbe 167K Apr 10 11:40 gkpStoreCreate<br>-rwxrwxr-x 1 urbe urbe 147K Apr 10 11:40 gkpStoreDumpFASTQ<br>-rwxrwxr-x 1 urbe urbe 184K Apr 10 11:41 greedyFragmentTiling<br>-rwxrwxr-x 1 urbe urbe 1,6K Apr 10 11:41 greedy_layout_to_IUM<br>-rwxrwxr-x 1 urbe urbe 142K Apr 10 11:40 initialTrim<br>-rwxrwxr-x 1 urbe urbe 967K Apr 10 11:41 jellyfish<br>-rwxrwxr-x 1 urbe urbe 219K Apr 10 11:41 markRepeatUnique<br>-rwxrwxr-x 1 urbe urbe 273K Apr 10 11:40 markUniqueUnique<br>-rwxrwxr-x 1 urbe urbe 114K Apr 10 11:40 mercy<br>-rwxrwxr-x 1 urbe urbe 3,8K Apr 10 11:41 mergeqc.pl<br>-rwxrwxr-x 1 urbe urbe 422K Apr 10 11:40 merTrim<br>-rwxrwxr-x 1 urbe urbe 125K Apr 10 11:40 merTrimApply<br>-rwxrwxr-x 1 urbe urbe 376K Apr 10 11:40 meryl<br>-rwxrwxr-x 1 urbe urbe 176K Apr 10 11:41 metagenomics_ovl_analyses<br>-rwxrwxr-x 1 urbe urbe 297K Apr 10 11:41 olap-from-seeds<br>-rwxrwxr-x 1 urbe urbe 275K Apr 10 11:41 outputLayout<br>-rwxrwxr-x 1 urbe urbe 229K Apr 10 11:41 overlapInCore<br>-rwxrwxr-x 1 urbe urbe 144K Apr 10 11:40 overlap_partition<br>-rwxrwxr-x 1 urbe urbe 179K Apr 10 11:41 overlapStats<br>-rwxrwxr-x 1 urbe urbe 179K Apr 10 11:41 overlapStore<br>-rwxrwxr-x 1 urbe urbe 153K Apr 10 11:41 overlapStoreBucketizer<br>-rwxrwxr-x 1 urbe urbe 175K Apr 10 11:41 overlapStoreBuild<br>-rwxrwxr-x 1 urbe urbe&nbsp; 33K Apr 10 11:41 overlapStoreIndexer<br>-rwxrwxr-x 1 urbe urbe&nbsp; 48K Apr 10 11:41 overlapStoreSorter<br>-rwxrwxr-x 1 urbe urbe 604K Apr 10 11:40 overmerry<br>lrwxrwxrwx 1 urbe urbe&nbsp;&nbsp;&nbsp; 4 Apr 10 11:41 pacBioToCA -&gt; PBcR<br>-rwxrwxr-x 1 urbe urbe 131K Apr 10 11:41 PBcR<br>-rwxrwxr-x 1 urbe urbe 2,9M Apr 10 11:41 pbdagcon<br>-rwxrwxr-x 1 urbe urbe 1,9M Apr 10 11:41 pbutgcns<br>-rwxrwxr-x 1 urbe urbe 201K Apr 10 11:40 remove_fragment<br>-rwxrwxr-x 1 urbe urbe 153K Apr 10 11:40 removeMateOverlap<br>-rwxrwxr-x 1 urbe urbe 2,5K Apr 10 11:41 replaceUIDwithName-fastq<br>-rwxrwxr-x 1 urbe urbe 1,2K Apr 10 11:41 replaceUIDwithName-posmap<br>-rwxrwxr-x 1 urbe urbe 1,3M Apr 10 11:41 resolveSurrogates<br>-rwxrwxr-x 1 urbe urbe 139K Apr 10 11:41 rewriteCache<br>-rwxrwxr-x 1 urbe urbe 232K Apr 10 11:41 runCA<br>-rwxrwxr-x 1 urbe urbe&nbsp; 88K Apr 10 11:41 runCA-dedupe<br>-rwxrwxr-x 1 urbe urbe&nbsp; 14K Apr 10 11:41 runCA-overlapStoreBuild<br>-rwxrwxr-x 1 urbe urbe 3,6K Apr 10 11:41 run_greedy.csh<br>-rwxrwxr-x 1 urbe urbe 297K Apr 10 11:40 sffToCA<br>-rwxrwxr-x 1 urbe urbe&nbsp; 13K Apr 10 11:40 show-corrects<br>-rwxrwxr-x 1 urbe urbe 557K Apr 10 11:41 splitUnitigs<br>-rwxrwxr-x 1 urbe urbe 1,4M Apr 10 11:41 terminator<br>drwxrwxr-x 2 urbe urbe 4,0K Apr 10 11:41 TIGR<br>-rwxrwxr-x 1 urbe urbe 526K Apr 10 11:41 tigStore<br>-rwxrwxr-x 1 urbe urbe&nbsp; 35K Apr 10 11:41 tracearchiveToCA<br>-rwxrwxr-x 1 urbe urbe&nbsp; 35K Apr 10 11:41 tracedb-to-frg.pl<br>-rwxrwxr-x 1 urbe urbe&nbsp; 44K Apr 10 11:41 trimFastqByQVWindow<br>-rwxrwxr-x 1 urbe urbe&nbsp; 18K Apr 10 11:40 uidclient<br>-rwxrwxr-x 1 urbe urbe 589K Apr 10 11:41 unitigger<br>-rwxrwxr-x 1 urbe urbe&nbsp; 42K Apr 10 11:40 upgrade-v8-to-v9<br>-rwxrwxr-x 1 urbe urbe&nbsp; 42K Apr 10 11:40 upgrade-v9-to-v10<br>-rwxrwxr-x 1 urbe urbe&nbsp; 854 Apr 10 11:41 utg2fasta<br>-rwxrwxr-x 1 urbe urbe 731K Apr 10 11:41 utgcns<br>-rwxrwxr-x 1 urbe urbe 561K Apr 10 11:41 utgcnsfix<br><br><br></p><p>Address of the bookmark: <a href="http://wgs-assembler.sourceforge.net/wiki/index.php/Main_Page" rel="nofollow">http://wgs-assembler.sourceforge.net/wiki/index.php/Main_Page</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/36373/tools-to-predict-the-impact-of-missense-variants</guid>
	<pubDate>Mon, 23 Apr 2018 12:57:33 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/36373/tools-to-predict-the-impact-of-missense-variants</link>
	<title><![CDATA[Tools to Predict the Impact of Missense Variants !]]></title>
	<description><![CDATA[<p><span>Prioritizing missense variants for further experimental investigation is a key challenge in current sequencing studies for exploring complex and Mendelian diseases. A large number of&nbsp;</span><em>in silico</em><span>&nbsp;tools have been employed for the task of pathogenicity prediction, including PolyPhen‐2, SIFT, FatHMM, MutationTaster‐2, MutationAssessor, Combined Annotation Dependent Depletion, LRT, phyloP, and GERP++, as well as optimized methods of combining tool scores, such as Condel and Logit. Due to the wealth of these methods, an important practical question to answer is which of these tools generalize best, that is, correctly predict the pathogenic character of new variants. </span></p><p><span>Study of 10 tools on five datasets that such a comparative evaluation of these tools is hindered by two types of circularity: they arise due to (1) the same variants or (2) different variants from the same protein occurring both in the datasets used for training and for evaluation of these tools, which may lead to overly optimistic results. Comparative evaluations of predictors that do not address these types of circularity may erroneously conclude that circularity confounded tools are most accurate among all tools, and may even outperform optimized combinations of tools.</span></p><p><span>Following tools are useful for mis sense muation detection ...&nbsp;</span></p><p>PolyPhen‐2 (PP2)<br />&ldquo;Predicts possible impact of an amino acid substitution on the structure and function of a human protein using straightforward physical and comparative considerations&rdquo;</p><p>MutationTaster‐2 (MT2)<br />&ldquo;Evaluation of the disease‐causing potential of DNA sequence alterations&rdquo;</p><p>MutationAssessor (MASS)<br />&ldquo;Predicts the functional impact of amino acid substitutions in proteins, such as mutations discovered in cancer or missense polymorphisms&rdquo;</p><p>LRT<br />&ldquo;Identify a subset of deleterious mutations that disrupt highly conserved amino acids within protein‐coding sequences, which are likely to be unconditionally deleterious&rdquo;</p><p>SIFT<br />&ldquo;Predicts whether an amino acid substitution affects protein function&rdquo;</p><p>GERP++<br />&ldquo;Identifies constrained elements in multiple alignments by quantifying substitution deficits. These deficits represent substitutions that would have occurred if the element were neutral DNA, but did not occur because the element has been under functional constraint. We refer to these deficits as &ldquo;rejected substitutions.&rdquo; Rejected substitutions are a natural measure of constraint that reflects the strength of past purifying selection on the element&rdquo;</p><p>phyloP<br />&ldquo;Compute conservation or acceleration P values based on an alignment and a model of neutral evolution&rdquo;</p><p>FatHMM unweighted (FatHMM‐U)<br />Predicts &ldquo;functional consequences of both coding variants, that is, nonsynonymous single‐nucleotide variants, and noncoding variants&rdquo;</p><p>FatHMM weighted (FatHMM‐W)<br />Predicts &ldquo;functional consequences of both coding variants, that is, nonsynonymous single‐nucleotide variants, and noncoding variants&rdquo; and its weighting scheme attributes higher tolerance scores to SNVs in proteins, related proteins, or domains that already include a high fraction of pathogenic variantsh</p><p>Combined Annotation Dependent Depletion (CADD)<br />&ldquo;CADD is a tool for scoring the deleteriousness of single‐nucleotide variants as well as insertion/deletions variants in the human genome&rdquo;</p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/pages/view/36398/tools-for-protein-protein-docking</guid>
	<pubDate>Wed, 25 Apr 2018 05:15:53 -0500</pubDate>
	<link>https://bioinformaticsonline.com/pages/view/36398/tools-for-protein-protein-docking</link>
	<title><![CDATA[Tools for Protein-Protein Docking !]]></title>
	<description><![CDATA[<p>Predicting the structure of protein&ndash;protein complexes using docking approaches is a difficult problem whose major challenges include identifying correct solutions, and properly dealing with molecular flexibility and conformational changes. Following are the tools to predict&nbsp;<span>the structure of protein&ndash;protein complexes:</span></p><p><a href="http://www.sbg.bio.ic.ac.uk/docking/index.html" target="_blank">3D-Dock Suite</a></p><p>Global rigid search: FFTShape complementarity and electrostatics</p><p>Re-scoring and clustering. Refinement of interface side-chains</p><p><a href="http://www.sbg.bio.ic.ac.uk/~3dgarden/" target="_blank">3D-Garden</a></p><p>Global rigid search in ensamble</p><p>Shape complementarity and Lennard&ndash;Jones potential</p><p>Side chain and backbone dihedral refinement</p><p><a href="http://www.sdsc.edu/CCMS/DOT/" target="_blank">DOT</a></p><p>Global rigid search: FFTShape complementarity, electrostatics and VDWNone</p><p><a href="http://users.unimi.it/~ddl/escherng/index.htm" target="_blank">Escher NG</a></p><p>Global rigid searchShape complementarity, hydrogen bonds and electrostatic</p><p>Integrated in&nbsp;<a href="http://users.unimi.it/~ddl/vega/download.htm" target="_blank">VEGA</a></p><p><a href="http://vakser.bioinformatics.ku.edu/resources/gramm/gramm1" target="_blank">GRAMM</a>&nbsp;</p><p>Global rigid search: FFT. smooth protein surface representation for soft docking</p><p>Shape complementarity and Lennard-Jones potential</p><p>Clustering of conformations</p><p><a href="http://vakser.bioinformatics.ku.edu/resources/gramm/grammx/" target="_blank">GRAMM-X</a>&nbsp;</p><p>Global rigid search: FFT. smooth protein surface representation for soft docking</p><p>Shape complementarity and Lennard-Jones potentialminimization and re-scoring with multiple filters</p><p><a href="http://www.loria.fr/~ritchied/hex_server/" target="_blank">HEX</a></p><p>Global rigid search: Fourier correlation of spherical harmonics</p><p>Shape complementarity</p><p><a href="http://www.csd.abdn.ac.uk/hex/" target="_blank"></a><a href="http://haddock.chem.uu.nl/Haddock/haddock.php" target="_blank">HADDOCK</a></p><p>Global rigid searchElectrostatic ,VDW and desolvation energy termsMD simulated annealing refinement . Filtering based on external data.&nbsp;</p><p><a href="http://www.molsoft.com/docking.html">ICM</a></p><p>Global rigid search: Monte CarloEmpirical scoring function</p><p>Clustering and selection of conformations. Refinement of interface side-chains and re-scoring</p><p><a href="http://www.weizmann.ac.il/Chemical_Research_Support/molfit/" target="_blank">MolFit&nbsp;</a></p><p>Global rigid search: FFTShape complementarity</p><p>Clustering of good solutions, filtering using&nbsp;<em>a priori&nbsp;</em>information and small, local rigid rotations around selected conformations</p><p><a href="http://bioinfo3d.cs.tau.ac.il/PatchDock/" target="_blank">PatchDock</a></p><p>Global rigid searchShape complementarity and atomic desolvation energy</p><p>Clustering of conformations</p><p><a href="http://inb.bsc.es/gn6/PyDock" target="_blank">PyDock</a></p><p>Global rigid search:FFTShape complementarity</p><p>rescoring by binding electrostatics and desolvation energy</p><p><a href="http://bioinfo3d.cs.tau.ac.il/PatchDock/" target="_blank"></a><a href="http://rosettadock.graylab.jhu.edu/" target="_blank">RosettaDock</a></p><p>Local rigid search: Monte Carlo with low and high resolution structure representation levels</p><p>Different scoring parameters for the different resolutions&nbsp;</p><p><a href="http://zlab.bu.edu/zdock/" target="_blank">ZDOCK</a></p><p>Global rigid search: FFTShape complementarity, desolvation energy, and electrostatics.</p><p>Energy minimization and re-scoringFree for academics</p><p>&nbsp;</p><p>Point to note:</p><p>The proper treatment of flexibility in protein&ndash;protein docking is still an active field of research. You first should analyzed your proteins in order to define their conformational space and then choose the most suitable method for your docking problem.</p>]]></description>
	<dc:creator>Poonam Mahapatra</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/36514/evidentialgene-tr2aacds-mrna-transcript-assembly-software</guid>
	<pubDate>Tue, 08 May 2018 04:39:39 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/36514/evidentialgene-tr2aacds-mrna-transcript-assembly-software</link>
	<title><![CDATA[EvidentialGene: tr2aacds, mRNA Transcript Assembly Software]]></title>
	<description><![CDATA[<p><span>EvidentialGene is a genome informatics project, "Evidence Directed Gene Construction for Eukaryotes", to construct high quality, accurate gene sets for animals and plants, developed by Don Gilbert at Indiana University, see</span><br><a href="http://arthropods.eugenes.org/EvidentialGene/" target="_blank">http://arthropods.eugenes.org/EvidentialGene/<span></span></a><br><br><span>Construction refers to the combination of classical gene prediction, and more recent gene assembly (de-novo and genome-assisted) methods. The basic Evigene methods involve using available best-of-breed gene prediction and assembly software, combining all evidence for genes, from expressed sequences, genome assembly sequences, related species protein sequences, and any other, to annotate and score gene constructions. Over-produced constructions are classified by gene evidence for best qualities per "locus", including genome-aligned and gene-transcript aligned (genome-free) locus identification. All software developed for EvidentialGene is publicly available. See project wiki/blog for notes.</span></p>
<p><span>Download&nbsp;</span></p>
<p>http://arthropods.eugenes.org/EvidentialGene/trassembly.html</p>
<p>https://sourceforge.net/p/evidentialgene/blog/</p><p>Address of the bookmark: <a href="http://arthropods.eugenes.org/EvidentialGene/trassembly.html" rel="nofollow">http://arthropods.eugenes.org/EvidentialGene/trassembly.html</a></p>]]></description>
	<dc:creator>Rahul Nayak</dc:creator>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/37520/mmgenome-tools-for-extracting-individual-genomes-from-metagneomes</guid>
	<pubDate>Thu, 09 Aug 2018 17:41:17 -0500</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/37520/mmgenome-tools-for-extracting-individual-genomes-from-metagneomes</link>
	<title><![CDATA[mmgenome: Tools for extracting individual genomes from metagneomes]]></title>
	<description><![CDATA[<p>The mmgenome toolbox enables reproducible extraction of individual genomes from metagenomes. It builds on the&nbsp;<a href="http://madsalbertsen.github.io/multi-metagenome/">multi-metagenome</a>&nbsp;concept, but wraps most of the process of extracting genomes in simple R functions. Thereby making the whole process of binning easy and at the same time reproducible through the Rmarkdown format.</p>
<p>The mmgenome R package also facilitates effortless integration with additional data sources and hence should not be seen as "yet another binning method", but rather a package to integrate different binning strategies.</p>
<p>All functions in the mmgenome R package has associated documentation, check it out in R by e.g.&nbsp;<code>?mmplot</code>.</p><p>Address of the bookmark: <a href="https://github.com/MadsAlbertsen/mmgenome" rel="nofollow">https://github.com/MadsAlbertsen/mmgenome</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
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