BOL: Related items

PeGAS: A Comprehensive Bioinformatic Solution for Pathogenic Bacterial Genomic Analysis

LEGE — Mon, 01 Sep 2025 01:18:10 -0500

This is PeGAS, a powerful bioinformatic tool designed for the seamless quality control, assembly, and annotation of Illumina paired-end reads specific to pathogenic bacteria. This tool integrates state-of-the-art open-source software to provide a streamlined and efficient workflow, ensuring accurate insights into the genomic makeup of pathogenic microbial strains.

Address of the bookmark: https://github.com/liviurotiul/PeGAS

Bioinformatics Training Courses At RASA LSI

RASA Life Sciences — Wed, 06 Nov 2019 00:30:51 -0600

RASA conducts comprehensive Life Science skill development training courses in Pune, India for working professionals, researchers, students and job-seeker. The trainings are crafted meticulously, covering different modules of courses such as Bioinformatics course, In silico Drug Discovery course, Next Generation Sequence data analysis course, Molecular Biology & Life science software development course wherein you learn from industry leaders how to apply these skills in life science & have a command over software developing process by using various methodologies. We conduct in-class training and instructor-led live online classes worldwide, along with corporate and skill development training worldwide.

Workshops are conducted in regular intervals on Drug Designing, Protein Modeling and Simulation, Chemoinformatics, Bioinformatics etc.The workshops are highly beneficial for working professionals, students, researcher for enhancements of the skills in short duration.

Single Cell RNAseq data analysis tutorial !!

Robert M Willioms — Mon, 27 Nov 2017 16:24:29 -0600

A major breakthrough (replaced microarrays) in the late 00’s and has been widely used since
Measures the average expression level for each gene across a large population of input cells
Useful for comparative transcriptomics, e.g. samples of the same tissue from different species
Useful for quantifying expression signatures from ensembles, e.g. in disease studies
Insufficient for studying heterogeneous systems, e.g. early development studies, complex tissues (brain)
Does not provide insights into the stochastic nature of gene expression

Following are the useful links:

Single Cell RNAseq data analysis Tutorial

A step-by-step workflow for low-level analysis of single-cell RNA-seq data

A step-by-step workflow for low-level analysis of single-cell RNA-seq data with Bioconductor

SCell: single-cell RNA-seq analysis software

https://github.com/diazlab/SCell

Beta-Poisson model for single-cell RNA-seq data analyses

https://github.com/nghiavtr/BPSC

Sincera: A Computational Pipeline for Single Cell RNA-Seq Profiling Analysis

https://research.cchmc.org/pbge/sincera.html

SC3 – consensus clustering of single-cell RNA-Seq data

http://biorxiv.org/content/early/2016/09/02/036558

Citrus: A toolkit for single cell sequencing analysis

http://biorxiv.org/content/early/2016/09/14/045070

Single-Cell Resolution of Temporal Gene Expression during Heart Development

http://www.cell.com/developmental-cell/fulltext/S1534-5807(16)30682-7

Scalable latent-factor models applied to single-cell RNA-seq data separate biological drivers from confounding effects

http://biorxiv.org/content/early/2016/11/15/087775

Single cell transcriptomes identify human islet cell signatures and reveal cell-type-specific expression changes in type 2 diabetes

http://genome.cshlp.org/content/early/2016/11/18/gr.212720.116.abstract

SCODE: An efficient regulatory network inference algorithm from single-cell RNA-Seq during differentiation

http://biorxiv.org/content/early/2016/11/21/088856

SCOUP is a probabilistic model to analyze single-cell expression data during differentiation

https://github.com/hmatsu1226/SCOUP

scLVM is a modelling framework for single-cell RNA-seq data

https://github.com/PMBio/scLVM

Selective Locally linear Inference of Cellular Expression Relationships (SLICER) algorithm for inferring cell trajectories

https://github.com/jw156605/SLICER

SinQC: A Method and Tool to Control Single-cell RNA-seq Data Quality

http://www.morgridge.net/SinQC.html

TSCAN: Pseudo-time reconstruction and evaluation in single-cell RNA-seq analysis

https://github.com/zji90/TSCAN

Visualization and cellular hierarchy inference of single-cell data using SPADE

http://www.nature.com/nprot/journal/v11/n7/full/nprot.2016.066.html

OEFinder: Identify ordering effect genes in single cell RNA-seq data

https://github.com/lengning/OEFinder

Machine learning training and courses in bioinformatics !

Rahul Nayak — Tue, 31 Dec 2019 19:33:07 -0600

Machine learning techniques have been successful in analyzing biological data because of their capabilities in handling randomness and uncertainty of data noise and in generalization. In this class, we will learn basics about probabilistic models and machine learning techniques. We will focus on probabilistic models (Markov models, Hidden Markov models, and Bayesian networks) for biological sequence analysis and systems biology. Other machine learning techniques, such as Naive bayes, neural networks and SVMs will only be covered briefly.

More at http://homes.sice.indiana.edu/yye/lab/teaching/spring2017-I529/

Delta: a new Web-based 3D genome visualization and analysis platform

Jit — Wed, 20 Dec 2017 08:49:55 -0600

Delta is an integrative visualization and analysis platform to facilitate visually annotating and exploring the 3D physical architecture of genomes. Delta takes Hi-C or ChIA-PET contact matrix as input and predicts the topologically associating domains and chromatin loops in the genome. It then generates a physical 3D model which represents the plausible consensus 3D structure of the genome. Deltafeatures a highly interactive visualization tool which enhances the integration of genome topology/physical structure with extensive genome annotation by juxtaposing the 3D model with diverse genomic assay outputs.

https://github.com/zhangzhwlab/delta

Address of the bookmark: https://github.com/zhangzhwlab/delta

Bioinformatician on Valentine's Day

BioQueen — Sun, 14 Feb 2021 11:36:32 -0600

Once asked to a bioinformatician "How is ur Valentine's Day?"

Bioinformatician replied:
if ($date == "Valentine's Day" && $me =! Bioinformatician) {
rose_day(); promise_day(); kiss_day();
}
else {
hack_genome(); ko-fi(); youtube(); do_scripting(); sleep();
)

MGcV: the microbial genomic context viewer for comparative genome analysis

Jit — Mon, 29 Jan 2018 04:55:46 -0600

MGcV is an interactive web-based visalization tool tailored to facilitate small scale genome analysis. To start using MGcV:

Supply your genes/genomic segments/phylogenetic tree of interest in the input-box by
- selecting the type of identifier and pasting identifiers (one per line)
- or by using the gene ID search tool
- or with the BLAST search tool
Click "Visualize context".

Consult the documentation to learn more about MGcV.

Address of the bookmark: http://mgcv.cmbi.ru.nl/

DBT BIOTECHNOLOGY ELIGIBILITY TEST (BET) 2020

Shruti Paniwala — Fri, 21 Aug 2020 09:17:24 -0500

Ministry of Science &Technology, Govt. of India

DBT-Junior Research Fellowship (DBT-JRF) in Biotechnology (2020)

BIOTECHNOLOGY ELIGIBILITY TEST (BET) 2020

Applications are invited from bonafide Indian citizens, residing in India for award of “DBT-Junior Research Fellowship” (DBT-JRF) for pursuing research in frontier areas of Biotechnology and Life Sciences. The candidates will be selected through “Biotechnology Eligibility Test (BET)”. Based on the performance in BET, two categories of merit list will be prepared (Category-I and Category-II). Government of India norms for reservation will be followed for selection. Candidates selected under category-I will be eligible to avail fellowship under the programme. These will be tenable at any University/Institute in India where the selected candidate registers for PhD Programme. Candidates selected under Category-II will be eligible to be appointed in any DBT sponsored project and avail fellowship from the project equivalent to NET/GATE, subject to selection through institutional selection process. There will be no binding on Principal Investigators of DBT sponsored projects to select JRF for their project from category-II list. Selection in category-II will not entitle student for any fellowship from DBT-JRF programme.

ELIGIBILITY

Qualification: M.Sc./ M.Tech./ M.V.Sc. or equivalent degree/ Integrated BS-MS/ B.E./ B.Tech. in any discipline of Biotechnology, M.Sc./ M.Tech. Bioinformatics/ Computational Biology, students admitted under DBT supported Postgraduate Teaching Programs. M.Sc. Life Science/ Bioscience/ Zoology/ Botany/ Microbiology/ Biochemistry/ Biophysics and Masters in Allied areas of Biology/Life Sciences. Candidates appearing in the final year examination are also eligible to apply.

Marks: Minimum 60% marks for General, EWS & OBC category and 55% for SC/ ST/ Differently abled in aggregate (or equivalent grade).

Age Limit: Upto 28 years as on the last date of application for General & EWS category. Age relaxation of up to 5 years (33 years) for SC/ ST/ Differently Abled/ women candidates and upto 3 years (31 years) for OBC (Non-Creamy Layer) candidates.

For detailed procedure for filling the application form, payment of application fee and uploading of required documents/ certificates in the prescribed format, please visit: http://rcb.res.in/BET2020. A non-refundable and non-transferable application fee of Rs. 1000/-is payable online by General/ OBC/ EWS candidates and Rs 250/- by SC/ ST/ Differently abled candidates.

IMPORTANT DATES

Online Registration Start	April 20, 2020
Online Registration Close	May 18, 2020
BET 2020	June 30, 2020 (Tuesday)* Tentative
Display of question paper and answer key on website	June 30, 2020
Last date of accepting representation of any discrepancy in Question paper & Answer key	July 03, 2020
Declaration of BET 2020 Result	July 20, 2020

KAT: a K-mer analysis toolkit to quality control NGS datasets and genome assemblies

Jit — Fri, 06 Jul 2018 03:36:45 -0500

KAT is a suite of tools that analyse jellyfish hashes or sequence files (fasta or fastq) using kmer counts. The following tools are currently available in KAT:

hist: Create an histogram of k-mer occurrences from a sequence file. Adds metadata in output for easy plotting.
gcp: K-mer GC Processor. Creates a matrix of the number of K-mers found given a GC count and a K-mer count.
comp: K-mer comparison tool. Creates a matrix of shared K-mers between two (or three) sequence files or hashes.
sect: SEquence Coverage estimator Tool. Estimates the coverage of each sequence in a file using K-mers from another sequence file.
blob: Given, reads and an assembly, calculates both the read and assembly K-mer coverage along with GC% for each sequence in the assembly.SEquence Coverage estimator Tool.
filter: Filtering tools. Contains tools for filtering k-mer hashes and FastQ/A files:
- kmer: Produces a k-mer hash containing only k-mers within specified coverage and GC tolerances.
- seq: Filters a sequence file based on whether or not the sequences contain k-mers within a provided hash.
plot: Plotting tools. Contains several plotting tools to visualise K-mer and compare distributions. The following plot tools are available:
- density: Creates a density plot from a matrix created with the "comp" tool. Typically this is used to compare two K-mer hashes produced by different NGS reads.
- profile: Creates a K-mer coverage plot for a single sequence. Takes in fasta coverage output coverage from the "sect" tool
- spectra-cn: Creates a stacked histogram using a matrix created with the "comp" tool. Typically this is used to compare a jellyfish hash produced from a read set to a jellyfish hash produced from an assembly. The plot shows the amount of distinct K-mers absent, as well as the copy number variation present within the assembly.
- spectra-hist: Creates a K-mer spectra plot for a set of K-mer histograms produced either by jellyfish-histo or kat-histo.
- spectra-mx: Creates a K-mer spectra plot for a set of K-mer histograms that are derived from selected rows or columns in a matrix produced by the "comp".

In addition, KAT contains a python script for analysing the mathematical distributions present in the K-mer spectra in order to determine how much content is present in each peak.

This README only contains some brief details of how to install and use KAT. For more extensive documentation please visit: https://kat.readthedocs.org/en/latest/

https://academic.oup.com/bioinformatics/article/33/4/574/2664339

Address of the bookmark: https://github.com/TGAC/KAT

Scientist B at AIIMS, New Delhi, Delhi

Thu, 03 Sep 2020 07:04:11 -0500

Scientist B at AIIMS, New Delhi, Delhi

Overview
Applications are invited from eligible candidates for the following position under Meity funded research project entitled: Artifical Intelligence in Oncology, Harnsessing big data and advanced computing to provide personalized diganosis and treatment for cancer patients purely on contractual basis

Scientist B

Salary: Rs.80,000/-

Qualification: 1st Class Masters Degree in Bioinformatics/ Computer Science/ Statistics with Ph.D in relevant subject from a recognized University with experience in Machine learning/ AI project plus two years research experience

Age: Upto 40 years

Details
Experience:2 Years
Location:New Delhi
Education:1st Class Masters Degree
SALARY: Rs.80,000/-
Key Skills: Research Fellowship
Desired Profile
Two years research experience

Company: AIIMS
All India Institute of Medical Sciences, New Delhi is a medical school, hospital and public medical research university

More at https://www.aiims.edu/en/notices/recruitment/aiims-recruitment.html?id=10844
PDF https://www.aiims.edu/images/pdf/recruitment/advertisement/Post_BioChem_22_08_20.PDF