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	<title><![CDATA[BOL: Related items]]></title>
	<link>https://bioinformaticsonline.com/related/44773?offset=490</link>
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	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31156/splitbam-splits-a-bam-by-chromosomes</guid>
	<pubDate>Tue, 28 Feb 2017 09:01:28 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31156/splitbam-splits-a-bam-by-chromosomes</link>
	<title><![CDATA[splitbam: splits a BAM by chromosomes]]></title>
	<description><![CDATA[<p><strong>splitbam</strong>&nbsp;splits a BAM by chromosomes.</p>
<p>Using the reference sequence dictionary (<code>*.dict</code>), it also creates some empty BAM files if no sam record was found for a chromosome. A pair of 'mock' SAM-Records can also be added to those empty BAMs to avoid some tools (like samtools) to crash.</p>
<h1>Usage</h1>
<p><code>java -jar splitbam.jar -p OUT/__CHROM__/__CHROM__.bam -R ref.fasta (bam|sam|stdin)</code></p>
<h1>Options</h1>
<ul>
<li>-h help; This screen.</li>
<li>-R (indexed reference file) REQUIRED.</li>
<li>-u (unmapped chromosome name): default:Unmapped</li>
<li>-e | --empty : generate EMPTY bams for chromosome having no read mapped</li>
<li>-m | --mock : if option '-e', add a mock pair of sam records to the empty bam</li>
<li>-p (output file/bam pattern) REQUIRED. MUST contain&nbsp;<strong><code>__CHROM__</code></strong>&nbsp;and end with .bam</li>
<li>-s assume input is sorted.</li>
<li>-x | --index create index.</li>
<li>-t | --tmp (dir) tmp file directory</li>
<li>-G (file) chrom-group file (see below)</li>
</ul><p>Address of the bookmark: <a href="https://code.google.com/archive/p/jvarkit/wikis/SplitBam.wiki" rel="nofollow">https://code.google.com/archive/p/jvarkit/wikis/SplitBam.wiki</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>
<item>
	<guid isPermaLink="true">https://bioinformaticsonline.com/bookmarks/view/31345/prokka-tool-for-the-rapid-annotation-of-prokaryotic-genomes</guid>
	<pubDate>Mon, 06 Mar 2017 03:49:57 -0600</pubDate>
	<link>https://bioinformaticsonline.com/bookmarks/view/31345/prokka-tool-for-the-rapid-annotation-of-prokaryotic-genomes</link>
	<title><![CDATA[Prokka: tool for the rapid annotation of prokaryotic genomes]]></title>
	<description><![CDATA[<p>Prokka is a software tool for the rapid annotation of prokaryotic genomes. A typical 4 Mbp genome can be fully annotated in less than 10 minutes on a quad-core computer, and scales well to 32 core SMP systems. It produces GFF3, GBK and SQN files that are ready for editing in Sequin and ultimately submitted to Genbank/DDJB/ENA.</p>
<p>&nbsp;</p><p>Address of the bookmark: <a href="http://www.vicbioinformatics.com/software.prokka.shtml" rel="nofollow">http://www.vicbioinformatics.com/software.prokka.shtml</a></p>]]></description>
	<dc:creator>Jit</dc:creator>
</item>

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