https://bioinformaticsonline.com/snippets/view/44493/python-script-to-convert-fastq-to-fasta? https://bioinformaticsonline.com/snippets/view/44493/python-script-to-convert-fastq-to-fasta Wed, 27 Mar 2024 20:30:32 -0500 https://bioinformaticsonline.com/snippets/view/44493/python-script-to-convert-fastq-to-fasta def fastq_to_fasta(fastq_file, fasta_file): with open(fastq_file, 'r') as fq: with open(fasta_file, 'w') as fa: while True: # Read four lines from the FASTQ file header = fq.readline().strip() sequence = fq.readline().strip() fq.readline() # Skip the '+' line quality = fq.readline().strip() # Check for EOF if not header: break # Write to the FASTA file fa.write('>' + header[1:] + '\n') fa.write(sequence + '\n') # Usage example fastq_to_fasta('input.fastq', 'output.fasta')]]> LEGE