Is reference genome necessary for gene expression study in transcriptome sequencing or for variant discovery in genome sequencing?

Rahul Agarwal — Wed, 17 Jul 2013 15:25:09 -0500

Like in case of plant genomes where nature of genome is too complex and huge in size to accomplish complete de novo assembly by current sequencing technology. What would be alternate solution? Can we live in reference free world?

BOL: Is reference genome necessary for gene expression study in transcriptome sequencing or for variant discovery in genome sequencing?

Is reference genome necessary for gene expression study in transcriptome sequencing or for variant discovery in genome sequencing?