Question: Question: How to predict miRNA targets?

pradyumna Jayaram
2879 days ago

Question: How to predict miRNA targets?

If i have a set of miRNA sequences, what are the tools that are available to predict the target genes? I tried miRanda and mirdb, are there any  tools or databases that do the same, as the rna is not already known i cant use targetscan etc. The tool must predict the target from the fasta sequence of miRNA.

Thank you

Answers
1

miRSearch is an online tool designed to quickly identify microRNAs (miRs) that target specific gene transcripts. miRSearch uses an advanced cross-referencing system to identify validated and predicted miRs for any target. miRSearch version 3.0 contains several new features: disease and tissue information, detailed microRNA information and updated target predictions.

Try miRSearch 3.0 now

Below is a list of other tools available for identifying or predicting links between microRNAs and their targets.

Table 1. MicroRNA target prediction tools. 
Method
Type of Method
Ref
Method Availability
Data availability
Resource
 
Stark et. al
Complementary
(Stark et. al., 2003)
Online search
Yes
 
miRanda
Complementary
(John et al., 2004)
Download
Yes
 
miRanda 
MiRBase
Complementary
(Enright et al., 2003)
Online search
Yes
 
Target Scan
Seed Complementary
(Lewis et al., 2005)
Online search
Yes
 
DIANA 
microT
Thermodynamics
(Kirakidou et al., 2004)
Download
Yes
 
PicTar
Thermodynamics
(Krek et al., 2005)
N/A
Yes
 
RNAHybrid
Thermodynamics & Statistical model
(Rehmsmeier et al., 2004)
Download
Yes
 
miRGen++
Baynesian Inference
(Huang et al., 2007b)
Mathlab Code
Yes
 
MiTarget
Support Vector Machine
(Kim et al. 2006)
Online search
Yes
 
MiRtaget2
Support Vector Machine
(Wang and El Naqa, 2008)
Online search
Yes
 
TarBase
Experimentally Validated Targets
(Sethupathy et al., 2006)
N/A
Yes