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Modular, efficient and constant-memory single-cell RNA-seq preprocessing
By Jit 1887 days ago Comments (1)pachterlab.github.io - With kallisto | bustools you can Generate a cell x gene or cell x transcript equivalence class count matrix Perform RNA velocity and single-nuclei RNA-seq analsis Quantify data from numerous technologies such as...StringTie Transcript assembly and quantification for RNA-Seq
By Jit 2187 days agoccb.jhu.edu - StringTie is a fast and highly efficient assembler of RNA-Seq alignments into potential transcripts. It uses a novel network flow algorithm as well as an optional de novo assembly step to assemble and quantitate full-length...Understanding RNA-Seq Normalization Methods: TPM vs. FPKM vs. CPM
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By Jit 3043 days agobioconductor.org - The dupRadar package gives an insight into the duplication problem by graphically relating the gene expression level and the duplication rate present on it. Thus, failed experiments can be easily identified at a glance
Is reference genome necessary for gene expression study in transcriptome sequencing or for variant discovery in genome sequencing?
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