Results for "Bioinformatics"
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RACA: Reference-Assisted Chromosome Assembly
Rreference-Assisted Chromosome Assembly (RACA), an algorithm to reliably order and orient sequence scaffolds generated by NGS and assemblers into longer chromosomal fragments using comparative genome information and paired-end reads. http://www.ncbi.nlm.nih.gov/pubmed/23307812 http://bioen-comp...Tags: Bioinformatics, NGS, Assembly, Reference, RACA, Reads
2944 days ago
PEAR: a fast and accurate Illumina Paired-End reAd mergeR
PEAR is an ultrafast, memory-efficient and highly accurate pair-end read merger. It is fully parallelized and can run with as low as just a few kilobytes of memory. PEAR evaluates all possible paired-end read overlaps and without requiring the target fragment size as input. In addition, it ...Tags: Bioinformatics, pair-end, read, merger, PEAR
2944 days ago
QUAST: quality assessment tool for genome assemblies
QUAST evaluates genome assemblies. For metagenomes, please see MetaQUAST project.It can works both with and without a given reference genome.The tool accepts multiple assemblies, thus is suitable for comparison. More at http://bioinf.spbau.ru/quast http://bioinformatics.oxfordjou...Tags: Bioinformatics, Computational Biology, Assembly, Comparison, Accuracy
2944 days ago
REAPR: a universal tool for genome assembly evaluation
REAPR is a tool that evaluates the accuracy of a genome assembly using mapped paired end reads, without the use of a reference genome for comparison. It can be used in any stage of an assembly pipeline to automatically break incorrect scaffolds and flag other errors in an assembly for manual insp...Tags: Bioinformatics, Computational Biology, Assembly, Comparison, Accuracy, Evaluation
2944 days ago
- SCALCE (/skeɪlz/, a.k.a. boosting Sequence Compression Algorithms using Locally ConsistentEncoding) is a tool for compressing FASTQ files. It is designed specifically for the Illumina-generated FASTQ files, but supports any valid FASTQ with consistent read lengths. ...
Tags: Bioinformatics, FASTQ, Compression, Decompression, NGS
2935 days ago
- Understanding Following table and graphs Duplication level kmer profile per base GC content per base N content per base quality per base sequence content per sequence GC content per sequence quality sequence length distribution More at http://www.bioinformatics.babraham.ac.uk/projec...
Tags: Bioinformatics, NGS, Assembly, Quality, FastQC, Tutorial
2935 days ago
Trimmomatic: A flexible read trimming tool for Illumina NGS data
Paired End: java -jar trimmomatic-0.35.jar PE -phred33 input_forward.fq.gz input_reverse.fq.gz output_forward_paired.fq.gz output_forward_unpaired.fq.gz output_reverse_paired.fq.gz output_reverse_unpaired.fq.gz ILLUMINACLIP:TruSeq3-PE.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36 ...Tags: Bioinformatics, Trim, NGS, Illumina, Reads, Trimmomatic
2935 days ago
- DISCOVAR is a new variant caller and DISCOVAR de novo a new genome assembler, both designed for state-of-the-art data. Their inputs are chosen to optimize quality while keeping costs low. Currently it takes as input Illumina reads of length 250 or longer — produced on MiSeq or HiSeq 2500 &m...
Tags: Bioinformatics, NGS, Assembly, DISCOVAR, Variants, BroadInstitute
2932 days ago
- SPAdes – St. Petersburg genome assembler – is intended for both standard isolates and single-cell MDA bacteria assemblies. This manual will help you to install and run SPAdes. SPAdes version 3.7.1 was released under GPLv2 on March 8, 2016 and can be downloaded from http://bioinf.spbau...
Tags: Bioinformatics, NGS, Assembly, Assembler, SPAdes, Reads, Illumina
2931 days ago
- High-throughput genomics has revolutionized biological research, however, while the number of sequenced genomes grows by the day, quality assessment of the resulting assembled sequences remains complicated and mostly limited to technical measures like N50. BUSCO provides measures for q...
Tags: Bioinformatics, Validation, Assembly, BUSCO, Orthologs, NGS
2910 days ago
