BBTools for bioinformatician !
...flag "-Xmx8g" and increase "max=" to something much higher.Alternatively, if these are assemblies and you're interested in continuity information (L50, N50, etc), you can run stats...2276 days ago
Whar are N50 and L50 of a contig assembly file? How to calculate it?
I am currently dealing with new contig assembly dataset, and bit confused about N50 and L50 stuff ....Tags: NGS, N50, L50, Contig, Assembly, Genome
2990 days ago
What is L50 in genome assembly?
N50 describes a sequence length whereas L50 describes a number of sequences. What am really confused ...Tags: L50, NGS, Genome, Assembly
2467 days ago
Comment on "LAMSA: fast split read alignment with long approximate matches"
...unless overriden. [null] (Illumina Moleculo) pacbio (PacBio SMRT): -i25 -l50 -m1 -M1 -O1,1,2,2 -E1,1,1,1 -w200 -b0 -e0.30 -d0.30 ont2d (Oxford Nanopore): -i25 -l50 -m1 -M1 -O1,1,1,1 -E1,1,1,1 -...2128 days ago