Results for "Reads"
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Bioinformatics tools for telomere to telomere assembly !
● Merfin – k-mer-based assembly and variant calling evaluation for improved consensus accuracy (Arang Rhie)● PanGenie – algorithm that leverages a pangenome reference built from haplotype-resolved genome assemblies in conjunction with k-mer count information from r...Tags: optimize, HiFi, reads, correct, tools
983 days ago
Steps to find palindrome in genomes !
Palindromes are sequences of nucleotides that read the same backward as forward. They can be present in genomes and have various biological functions. Here are some methods for discovering palindromes in genomes: Direct sequence search: One of the simplest ways to discove...Tags: Palindromes, sequences, nucleotides, reads, backward, forward, methods, steps
415 days ago
Software and Tools to detect structure variation with long reads !!
Uncovering the connection between genetics and heritable diseases requires an approach that looks at all the variant bases and types in a genome. While a PacBio de novo assembly resolves the most novel SV variants. 8-10X PacBio coverage of single genomes or trios reveals triple the SVs ...Tags: Bioinformatics, LR, Long, Reads, Tools, Software, Pacbio, Genome, DNA, Sequence, NGS, SV, Variation, Structure
2599 days ago
BBTools for bioinformatician !
BBMap.sh Mapping Nanopore reads BBMap.sh has a length cap of 6kbp. Reads longer than this will be broken into 6kbp pieces and mapped independently. Code: $ mapPacBio.sh -Xmx20g k=7 in=reads.fastq ref=reference.fa maxlen=1000 minlen=200 idtag ow int=f qin=33 out=mapped1.sam minratio=0.15...Tags: BBMap, Map, NGS, Reads, Sam, Bam, Contamination, Filtration
2262 days ago
Protocol for De novo Genome Assembly using Illumina Reads
In this protocol, we address and describe the de novo assembly method for small to medium-sized genomes. What is de novo genome assembly?The method of taking a large number of short DNA sequences and placing them back together to create a reflection of the original chromosomes from which the DNA...Tags: Protocol, De novo, Genome, Assembly, Illumina, Reads
1196 days ago
Common steps for reads mapping !
Mapping reads to a reference genome is an essential step in many types of genomic analysis, such as variant calling and gene expression analysis. Here are some general steps to follow for mapping reads to a genome: Choose a read mapper: There are many read mappers availab...Tags: Reads, Mapping, Steps, Methods, NGS
415 days ago
