ruchira |
Question: Annotating a sequence not yet published
I have 198,991 bp long sequence. I ran it through Maker, Glimmer and GeneMarkS. All three gave me different number of predicted genes.Here I am talking about genes that I got through GeneMarkS.
>gene_id_14 with this gene, i got the following result
Now if I combine genes 13 and 14. I got
what does that suggest? Also, how to find the correct number of predicted genes when all three tools give different results? Thanks |
Hi Ruchira,
I Think There is Different software using Different Algorithm that's why something result look like different.But u can use GENESCAN. GENESCAN gives accurate result and also used world wide gene prediction tool.
GENESCAN : http://genes.mit.edu/GENSCAN.html
Thank you.
Hi Ruchira,
It looks confusing to me as well, and question remain WHY? I guess it might be due to parameter differences. I will suggest you to please read this manuscript entitled "A beginner’s guide to eukaryotic genome annotation" http://fasta.bioch.virginia.edu/cshl/pdf/12/ajm12/euk_genome_annotation_review.pdf . I hope it will be helpful for your research point of view.
Exercise at http://bix.ucsd.edu/bioalgorithms/problems/03_asmt.htm
Thanks