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Question: Question: What are the difference between 3C, 4C and Hi-C methods ?

Rahul Nayak
2253 days ago

Question: What are the difference between 3C, 4C and Hi-C methods ?

3C: chromosome conformation capture

4C:chromosome conformation capture-on-chip

5C : chromosome conformation capture carbon copy

Answers
0

In general intereration point of view, the 3C probe for an interaction between two specific regions that you identify a priori. In 4C, one fragment of interest is known, and you probe for all the potential fragments interacting with your fragment of interest.

We are using Hi-C in genome assembly and scaffolding. The Hi-C is an unbiased method that can identify all potential fragment interactions without any a priori knowledge of the interacting fragments.

I will recommend you to read this http://genesdev.cshlp.org/content/26/1/11.full.pdf+html for better understanding of the terms.