If you are looking for any online tool then try Alternative Splice Site Predictor (ASSP) which predicts putative alternative exon isoform, cryptic, and constitutive splice sites of internal (coding) exons. http://wangcomputing.com/assp/index.html
There are several other tools available for such purposes, please visit http://www.phenosystems.com/www/index.php/links-to-various-tools-and-information/splice-prediction-tools
Hi Tayyiba,
Sorry, you did not explain you question well. I guess your are looking for Alternative splicing and functional prediction analysis tool/script.
You can use "AltAnalyze" which is a freely available, open-source and cross-platform program. Please find the source code at http://code.google.com/p/altanalyze/
http://code.google.com/p/altanalyze/wiki/SetPythonPATH
For visualizing alternative splicing and the effects of splicing quantitative trait loci, you can use or SplicePlot http://montgomerylab.stanford.edu/spliceplot/
Thanks