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  • Frequent Paired-end reads (PE 2x100) mapping command lines

    bowtie2 -x hs37m -X 650 -q -1 r1.fq -2 r2.fq -S r12.bowtie2.sam bwa aln hs37m.fa r1.fq > r1.sai && bwa aln hs37m...ai \ && bwa sampe hs37m r1.sai r2.sai r1.fq r2.fq > r...gsnap -A sam -d hs37m r1.fq r2.fq > r12.gsnap.sam novoalign -r...

    2193 days ago

  • Ancestral sequence reconstruction steps !

    ...idea of protein 'resurrection' was suggested in 1963 by Pauling and Zuckerkandl...ucture. but, I suggest following steps?   1- Alignments "Mafft - htt...des, I perform with ---recombination   4.1- Phi-test, implemented in Spl...

    2190 days ago

  • Gap filling or Contigs extensions tools !

    ...-a-wide-range-of-bioinformatics-solutions/sspacev12/), ATLAS tools (http://www.h...ok at the IMAGE tool: http://genomebiology.com/2010/11/4/R41 Also SOAPdenovo...(b). d. Trim C' so it does not extend more than 100 kb to either end of C0. Set...

    2176 days ago

  • LINKS scaffolder bloomfilter setting !

    ...xrwxr-x 3 urbe urbe 4096 Jun 15 12:15 lib -rwxrwxrwx 1 urbe ur...har -O3 BloomFilter_wrap.cxx:1892:30: fatal error: ../BloomF...(FILE*)’: ../BloomFilter.hpp:141:59: warning: ignoring retur...eparated by comma. eg. -d 500,1000,2000,3000 -k k-mer value...

    2162 days ago

  • Understanding BLASTn output format 6 !

    ...t length mismatch gapopen qstart qend sstart send evalue bitscore  1.  qseqid  query (e.g., gene...lignment in query  9.  sstart  start of alignment in subject  10.  send  end of alignment i...

    2150 days ago

  • Interview Puzzles for Bioinformatician !

    ...been asked in top Tech Interview. 2 Eggs and 100 Floor Classic Puzzle Five...Puzzle Will you accept the bet? The Puzzle of 100 Hats Man fell in Well Puzz...r Microsoft Interview Puzzles, you may refer,Top 15 Microsoft Interview PuzzlesM...

    2130 days ago

  • List of non-commercial NGS genotype-calling software

    ...nt database (for example, dbSNP) Package for NGS data analysis, which includes a single individual genotype caller (SOAPsnp) realSFS http://128.32.118.212/thorfinn/realSFS/ Sing...

    2107 days ago

  • Parallel Processing with Perl !

    ...eal with huge datasets which  are more than 100GB size. The traditional way...ting them. Putting it all together, If you have 100GB data you can do this s...generates, since it does take some time(remember 100GB). I think now you can use...

    2091 days ago

  • Benchmarking Perl Module !

    ...by our perl program. This is the simple way to use the module Example1: use Benchmark; $first_time...icated way: use Benchmark;sub first { my(arguments) = @_; } timethese(100, { first => ‘first...

    2091 days ago

  • Industrial Training in Computer Aided Drug Designing (CADD)

    ...ter Aided Drug Designing (CADD)!!! #rasalsi #rasa In our Industrial program you will get Knowledge on RNA Seq, CHIP Seq, Batch Starts From 18th November 2019 #hurryup #registernow #enqu...

    1646 days ago