Tools to Predict the Impact of Missense Variants !
...tion” GERP++“Identifies constrained elements in multiple alignments by quantifying substitution...hyloP“Compute conservation or acceleration P values based on an alignment and a model of neutral evolut...2211 days ago
Ancestral sequence reconstruction steps !
...ion. BEAST is the best way to predict the ancestral structure. but, I suggest following steps? 1- Alignments "Mafft - http://mafft.cbrc.jp/alignment/software/source.html" mafft...2187 days ago
Understanding BLASTn output format 6 !
...age of identical matches 4. length alignment length 5. mismatch ...of gap openings 7. qstart start of alignment in query 8. qend en... Subject sequence lengthqstart Start of alignment in queryqend End of alig...2146 days ago