fragScaff: Genome Assembly with Contiguity Preserving Transposition
Contiguity preserving transposition and sequencing (CPT-seq) is an entirely in vitro means of generating libraries comprised of 9216 indexed pools, each of which contains thousands of sparsely sequenced long fragments ranging from 5 kilobases to >1 megabase. This software, fragScaff, leverages...Tags: fragScaff, Genome, Assembly, Contiguity, Preserving, Transposition, Genome, Assembly
2176 days ago
auN: a new metric to measure assembly contiguity
Given a de novo assembly, we often measure the “average” contig length by N50. N50 is neither the real average nor median. It is the length of the contig such that this and longer contigs cover at least 50% of the assembly. A longer N50 indicates better contiguity. We can si...Tags: auN, new, metric, measure, assembly, contiguity
635 days ago