KAD is designed for evaluating the accuracy of nucleotide base quality of genome assemblies. Briefly, abundance of k-mers are quantified for both sequencing reads and assembly sequences. Comparison of the two values results in a single value per k-mer, K-mer Abundance Difference (KAD), which indi...
The abundance of a species in a dataset is considered as the fraction of reads that belong to that species. For example, if there is a dataset with 10,000,000 reads and 1,000,000 of them belong to E. coli, then the abundance of E. coli will be 0.1.